lila moradi; raouf seyed sharifi
Abstract
In order to study the effects of plant growth promoting rhizobacteria (PGPR) and salinity stress on seed germination parameters, K and Na content of rye (Secale cereal L.) seedling , two factorial experiments based on CRD and RCB design were conducted with three replications at laboratory and greenhouse ...
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In order to study the effects of plant growth promoting rhizobacteria (PGPR) and salinity stress on seed germination parameters, K and Na content of rye (Secale cereal L.) seedling , two factorial experiments based on CRD and RCB design were conducted with three replications at laboratory and greenhouse respectively at faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabil in 2016. Experimental factors were included soil salinity in four levels (no-salt as control, salinity 25, 50 and 75 mM as NaCl) and seed inoculation with plant growth promoting rhizobacteria in four levels (no inoculation as control, inoculation with Pseudomonas, Azosprilium, both application of Pseudomonas and Azosprilium). The results showed that maximum germination components (such as ridicule and plumule length, radicule and plumule dry weight, germination percentage, uniformity of germination) were obtained at co-application of Pseudomonas and Azosprilium under non-saline condition and minimum of these parameters were obtained at 75 Mm NaCl and no inoculation. K/Na ratio in root and shoot were decreased with increasing salinity level. It was vise versa in seed inoculation with plant growth promoting rhizobacteria. Maximum ratio of Na/K was obtained at the highest level salinity and no inoculation seed with PGPR and minimum of it was obtained in both inoculation with Pseudomonas and Azosprilium and the least level of salinity. It seems that application of PGPR can be used as a proper method for increasing K /Na ratio, germination components and seedling growth of rye under salinity stress.
S.R. Khatami; M. Sedghi; R. Seyed Sharifi
Abstract
In this research effect of priming types was investigated on the activity of antioxidant enzymes including Catalase (CAT), Ascorbate peroxidase (APX), Superoxide dismutase (SOD), Malone dialdehyde (MDA) and Gluthation reductase (Gred) in hybrid maize single cross 704 under drought stress. Treatments ...
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In this research effect of priming types was investigated on the activity of antioxidant enzymes including Catalase (CAT), Ascorbate peroxidase (APX), Superoxide dismutase (SOD), Malone dialdehyde (MDA) and Gluthation reductase (Gred) in hybrid maize single cross 704 under drought stress. Treatments were drought at 4 levels (0, -3, -6 and -9 bar) and priming (Hormone, hydro, osmo, ascorbic and control). Germination percentage, seedling vigor and the activity of antioxidant enzymes were measured. Results showed that interaction of drought and priming was significant an all traits except of germination percentage. The highest seedling vigor (15.996) obtained from control condition. The highest CAT activity (35 μmol min-1 mg-1) observed in ascorbic acid priming at -9 bar drought stress. Also, the highest APX activity was related to hormone priming and -9 bar. While the lowest activity observed in control drought and osmo-priming. The highest amount of MDA achieved in -9 bar and control priming. The highest SOD activity observed in -9 bar. The highest Gred activity obtained by ascorbic acid priming at -9 bar.
L. Yari; M. Sedghi; A. Hamidi; R. Seyed Sharifi
Abstract
This study was conducted to investigate the role of water and polyamines (PAs) as priming agents on improving seed vigour and chilling tolerance with different moisture content (Mc)(14.0,16.0,18.0%) under chilling conditions in barley (Hordeum vulgare L.) c.v ...
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This study was conducted to investigate the role of water and polyamines (PAs) as priming agents on improving seed vigour and chilling tolerance with different moisture content (Mc)(14.0,16.0,18.0%) under chilling conditions in barley (Hordeum vulgare L.) c.v Usef. Experiments were conducted at the seed and Plant certification and Registration Research Institute in 2013 -2014. The seeds were hand harvested at three initial moisture contents including 18, 16, and 14% wet weight basis .The seed samples then were sealed in polythene bags and stored in conditioned storage and equilibrium for 8 months. After that for seed priming, seeds were soaked in 20mg/L-1 (w/v) aerated solution of spermine (Spm), Puterscine (Put), and Spermidine (Spd) and distilled water (W) for 16h at 20±2C°. After each treatment, Seeds dried to original moisture level in forced air and sealed in polythene bags, stored in a conditioned storage again until one month. Experimental units were arranged as factorial in a completely randomized design with four replications after treat. The results indicated that Mc18% improved the germination percentage (GP) and seedling vigour compared to Mc 16 and 14%. The electrolyte leakage decreased in seeds harvested by Mc 18% when compared with Mc16 and Mc 14.0%. Also seed treatments significantly increased the seed viability. Likewise, in chilling stress condition maximum proline content and areal dry weight were obtained from Put treat, whereas the lowest electrolyte leakage was recorded for Put treat. Meanwhile, interaction between seed moisture content ×PAs treatment in cold condition significantly affected on plumule length and radicle dry weight, statistically maximum plumule length and radicle dry weight were recorded from Put ×18.0% mc. Also seed priming with water had positive effect on seedling growth after chilling stress. Generally, the effectiveness of PAs on improving chilling tolerance and increasing seedling vigor was more pronounced in Put treatment along with sample with 18.0% mc.
L. Yari; M. Sedghi; A. Hamidi; R. Seyed Sharifi
Volume 5, Issue 2 , January 2016, , Pages 37-50
Abstract
This study was conducted to investigate the role of polyamines (PAs) as priming agents on improving seed vigour with different moisture content (Mc)(14,16,18%) under storage conditions in barley (Hordeum vulgare L.) c.v Usef. Experiments were conducted at the Seed and Plant Certification ...
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This study was conducted to investigate the role of polyamines (PAs) as priming agents on improving seed vigour with different moisture content (Mc)(14,16,18%) under storage conditions in barley (Hordeum vulgare L.) c.v Usef. Experiments were conducted at the Seed and Plant Certification and Registration Research Institute 1n 2012 -2013. The seeds were hand harvested at three initial moisture contents including 18, 16, 14% using wet weight basis. The seed samples then were sealed in polythene bags and stored in conditioned storages and for 8 months. After that for seed priming, seeds were soaked in 20mg/L-1 (w/v) aerated solution of Spermine (Spm), Puterscine (Put), and Spermidine (Spd) and distilled water (W) for 16h at 20±2C°. After each treatment, seeds were dried to original moisture level in forced air and sealed in polythene bags, stored in a conditioned storage again for one month. Experimental units were arranged as a completely randomized design with four replications after harvesting time and one month conditioned storage after treatment. The result indicated that 18% MC improved the germination percentage and seedling vigour compared to 16 and 14 % MC. Also after 8 month storage, 18% Mc had the highest germination percentage and seedling vigour. The electrolyte leakage decreased in seeds with 18% MC compared to 16 and 14% MC. Also seed treatments significantly increased the seed viability and germination percentage, whereas, mean germination time and electrical conductivity were reduced in all seed treatments. Meanwhile, maximum germination percentage was detected for seed Spm seed treatment and 18% MC. Generally by decreasing moisture content of seed and delay in harvesting time, seedling vigour decreased and the optimum moisture content and seed treatment were 18% and Spm, respectively.
R. Seyed Sharifi; F. Lotfollah; M. Sedghi; R. Seyed Sharifi
Volume 5, Issue 1 , March 2015, , Pages 15-26
Abstract
In order to investigate the effects of free living nitrogen fixing bacteria application and time of nitrogen spraying on contribution of stem reserves in grain yield, rate and effective grain filling period of Triticale, a factorial experiment was conducted based on a randomized complete block design ...
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In order to investigate the effects of free living nitrogen fixing bacteria application and time of nitrogen spraying on contribution of stem reserves in grain yield, rate and effective grain filling period of Triticale, a factorial experiment was conducted based on a randomized complete block design with three replications in Research farm of the Faculty of Agriculture, University of Mohaghegh Ardabili in 2012. Treatments consisted of different nitrogen spraying times in four levels (no spraying as control, spraying in boot stage, ear emergence, grain filling period) and seed inoculation with plant growth promoting rhizobacteria in four levels containing (without inoculation as control, seed inoculation with Azotobacter chroococcum strain 5, Azospirillum lipoferum strain OF, Psedomunas putida strain 9). Results indicated that spraying time of nitrogen fertilizer and seed inoculation with free living nitrogen fixing bacteria had significant effects on yield, yield components, rate and effective grain filling period of Triticale. Maximum of grain weight (0/054 gr) and effective grain filling period (34.17 days) were obtained in seed inoculation with Azotobacter× nitrogen spraying in boot stage. Maximum of contribution of stem reserves in grain yield (30.63 %) was obtained in no nitrogen spraying × no seed inoculation with PGPR and minimum of it (8.12 and 8.13 %) were obtained in nitrogen spraying in boot stage × seed inoculation with Azotobacter and spraying in ear emergence × seed inoculation with Azotobacter. It seems that in order to increase the grain yield,rate and effective grain filling period of Triticale, it can be suggested that nitrogen spraying is applied in boot stage and seed inoculation with Azotobacter
