Haniyeh Saadat; Mohammad Sedghi
Abstract
In order to investigate The effect of priming and aging on the growth indicators and activity of antioxidant enzymes in hybrid maize single cross 704 a factorial experiment was conducted based on a completely randomized design at the University of Mohaghegh Ardabili in 2021 with 3 replications. Treatments ...
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In order to investigate The effect of priming and aging on the growth indicators and activity of antioxidant enzymes in hybrid maize single cross 704 a factorial experiment was conducted based on a completely randomized design at the University of Mohaghegh Ardabili in 2021 with 3 replications. Treatments were aging (control, 7 and 14 days) and priming (control, hydro-priming, priming by gibberellin (20 mg / l) and salicylic acid (100 mg / l)). The results showed that aging decreased growth indicators including Radicle Length (RL), Plumule Length (PL), Seedling Length (SL), Radicle Dry Weight (RDW), Plumule Dry Weight (PDW), Radicle Fresh Weight (RFW), Plumule Fresh Weight (PFW) Seed Length Vigor Index (SLVI) and Seed Weight Vigor Index (SLWI), but types of priming, especially priming with gibberellin improved these traits. As compared to the control, the catalase, superoxide dismutase, and glutathione reductase activities increased respectively by 73%, 63%, and 21% after gibberellin treatment and 14 days of aging. The highest amount of peroxidase enzyme activity (1.797 units mg-1 protein) was related to gibberellin priming. In general, gibberellin pretreatment increased fragile corn seeds' growth indicators and antioxidant enzymes activities, and it can be used to enhance.aging compared to the control showed an increase respectively about 73%, 63% and 21%. The highest amount of peroxidase enzyme activity (1.797 units mg-1 protein) was related to gibberellin priming. In general, using gibberellin pretreatment strengthened weak corn seeds growth indicators and antioxidant enzymes and the treatment can be used to increase weak seed vigor.
Tayyebeh Saadat; Mohammad Sedghi; Abdolghayyoum Gholipouri; Raouf Seyed Sharifi; Roghayyeh Sheikhbaglou
Abstract
To examine the effect of priming and deterioration on the activity of antioxidant enzymes, mobility of seed reserves in French bean, a factorial experiment was performed in a completely randomized design with three replications. Treatments included deterioration (control without deterioration, 88% and ...
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To examine the effect of priming and deterioration on the activity of antioxidant enzymes, mobility of seed reserves in French bean, a factorial experiment was performed in a completely randomized design with three replications. Treatments included deterioration (control without deterioration, 88% and 78% of germination of control) and priming (control, hydro-priming, priming by gibberellin and salicylic acid). The results showed that deterioration reduced germination percentage. Priming reduced the effect of deterioration and improved germination percentage. By increasing deterioration, seed reserves use efficiency (SRUE), seed reserves remobilization efficiency (SRRE) and fraction of used seed reserves (FUSR) were reduced, but respiration index (SR) and residual seed dry weight (RSDW) were increased. SRUE reduction was about 30% compared to the control treatment of deterioration and seed respiration index in gibberellin pre-treatment was higher about 24% compared to the control treatment without priming. The total seed protein content in gibberellin pretreatment and without deterioration was increased about 32 percent. The peroxidase activity in gibberellin treatment and deterioration 88% compared to the control showed an increase about 57%. The most superoxide dismutase activity (SOD, 175.2 unit mg-1 protein) was observed in priming with salicylic acid and deterioration 88%. The maximum amount of malondialdehyde (MDA, 1.46 mmol g-1 FW) was related to the treatment with no priming and deterioration 78%. In general, using gibberellin pretreatment strengthened weak bean seeds physiologically and the treatment can be used to increase weak seed vigor.
Iman Nemati; Mohammad Sedghi; Ghasem Hoseini Salekdeh; Reza Tavakkol afshari; Mohammadreza Naghavi
Abstract
Common cocklebur (Xanthium strumarium L.) is a widespread weed with high medical value and interested for researchers because of deep dormancy in one of its two seeds in one bur. However, lack of genomic data has led to low information about it. Transcriptome large and small seeds were sequenced using ...
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Common cocklebur (Xanthium strumarium L.) is a widespread weed with high medical value and interested for researchers because of deep dormancy in one of its two seeds in one bur. However, lack of genomic data has led to low information about it. Transcriptome large and small seeds were sequenced using Illumina platform to identify and functional analysis of differentially expressed transcripts in two seeds. Identified sequences in each seeds were compared and differentially expressed genes were functionally annotated. In this research 191192 sequence with a mean of 989.69 bp were detected. Sequence similarity analysis and functional analysis was carried out aginst nr, GO and KEGG databases. Differentially expressed genes had the most similarity with sunflower (83.41 per cent) in terms of top hits. GO analysis led to identify 615 functional annotation distributed in 36 categories. The most abomdant GO in biological process was biosynthesis. Results of our research shows the higher biosynthetic and metabolic processes in large seed of certain bur rather than the small one and also key regulatory role of transcription during seed development.
S.R. Khatami; M. Sedghi; R. Seyed Sharifi
Abstract
In this research effect of priming types was investigated on the activity of antioxidant enzymes including Catalase (CAT), Ascorbate peroxidase (APX), Superoxide dismutase (SOD), Malone dialdehyde (MDA) and Gluthation reductase (Gred) in hybrid maize single cross 704 under drought stress. Treatments ...
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In this research effect of priming types was investigated on the activity of antioxidant enzymes including Catalase (CAT), Ascorbate peroxidase (APX), Superoxide dismutase (SOD), Malone dialdehyde (MDA) and Gluthation reductase (Gred) in hybrid maize single cross 704 under drought stress. Treatments were drought at 4 levels (0, -3, -6 and -9 bar) and priming (Hormone, hydro, osmo, ascorbic and control). Germination percentage, seedling vigor and the activity of antioxidant enzymes were measured. Results showed that interaction of drought and priming was significant an all traits except of germination percentage. The highest seedling vigor (15.996) obtained from control condition. The highest CAT activity (35 μmol min-1 mg-1) observed in ascorbic acid priming at -9 bar drought stress. Also, the highest APX activity was related to hormone priming and -9 bar. While the lowest activity observed in control drought and osmo-priming. The highest amount of MDA achieved in -9 bar and control priming. The highest SOD activity observed in -9 bar. The highest Gred activity obtained by ascorbic acid priming at -9 bar.
L. Yari; M. Sedghi; A. Hamidi; R. Seyed Sharifi
Abstract
This study was conducted to investigate the role of water and polyamines (PAs) as priming agents on improving seed vigour and chilling tolerance with different moisture content (Mc)(14.0,16.0,18.0%) under chilling conditions in barley (Hordeum vulgare L.) c.v ...
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This study was conducted to investigate the role of water and polyamines (PAs) as priming agents on improving seed vigour and chilling tolerance with different moisture content (Mc)(14.0,16.0,18.0%) under chilling conditions in barley (Hordeum vulgare L.) c.v Usef. Experiments were conducted at the seed and Plant certification and Registration Research Institute in 2013 -2014. The seeds were hand harvested at three initial moisture contents including 18, 16, and 14% wet weight basis .The seed samples then were sealed in polythene bags and stored in conditioned storage and equilibrium for 8 months. After that for seed priming, seeds were soaked in 20mg/L-1 (w/v) aerated solution of spermine (Spm), Puterscine (Put), and Spermidine (Spd) and distilled water (W) for 16h at 20±2C°. After each treatment, Seeds dried to original moisture level in forced air and sealed in polythene bags, stored in a conditioned storage again until one month. Experimental units were arranged as factorial in a completely randomized design with four replications after treat. The results indicated that Mc18% improved the germination percentage (GP) and seedling vigour compared to Mc 16 and 14%. The electrolyte leakage decreased in seeds harvested by Mc 18% when compared with Mc16 and Mc 14.0%. Also seed treatments significantly increased the seed viability. Likewise, in chilling stress condition maximum proline content and areal dry weight were obtained from Put treat, whereas the lowest electrolyte leakage was recorded for Put treat. Meanwhile, interaction between seed moisture content ×PAs treatment in cold condition significantly affected on plumule length and radicle dry weight, statistically maximum plumule length and radicle dry weight were recorded from Put ×18.0% mc. Also seed priming with water had positive effect on seedling growth after chilling stress. Generally, the effectiveness of PAs on improving chilling tolerance and increasing seedling vigor was more pronounced in Put treatment along with sample with 18.0% mc.
S. Ghahremani; M. Sedghi; R. Seyed Sharifi
Abstract
In order to investigate the effects of seed deterioration on the activity of antioxidant enzymes and lipid peroxidation in pumpkin seedlings a factorial experiment conducted based on completely randomized design with three replications. Treatments were three deterioration levels (control, 5 and ...
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In order to investigate the effects of seed deterioration on the activity of antioxidant enzymes and lipid peroxidation in pumpkin seedlings a factorial experiment conducted based on completely randomized design with three replications. Treatments were three deterioration levels (control, 5 and 10 days deterioration at 45◦C and 95-100% relative humidity) and three germination temperatures (15, 25 and 35°C). Results showed that activity of superoxide dismutase and catalase were the highest at 5 days aging and 25°C, and activity of peroxidase was the highest at non-aging and 25°C. Also, the highest amount of Malondialdehyde (7.1 mmol gr-1FW) observed in the seeds with 10 days deterioration and 15°C. The highest respiration index (0.018 mg) achieved in 5 days deterioration and 35°C and the lowest value (0.009 mg) was related to 10 days deterioration and 35°C. It seems that reduction in seed vigor under deterioration and high temperatures causes to oxidative stress and lipid peroxidation and leads to increase in the activity of antioxidant enzymes in order to face the related damages. Considering results obtained from germination and antioxidant enzymes activity the best temperature for germination of aged seeds of pumpkin was 15◦C.
L. Yari; M. Sedghi; A. Hamidi; R. Seyed Sharifi
Volume 5, Issue 2 , January 2016, , Pages 37-50
Abstract
This study was conducted to investigate the role of polyamines (PAs) as priming agents on improving seed vigour with different moisture content (Mc)(14,16,18%) under storage conditions in barley (Hordeum vulgare L.) c.v Usef. Experiments were conducted at the Seed and Plant Certification ...
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This study was conducted to investigate the role of polyamines (PAs) as priming agents on improving seed vigour with different moisture content (Mc)(14,16,18%) under storage conditions in barley (Hordeum vulgare L.) c.v Usef. Experiments were conducted at the Seed and Plant Certification and Registration Research Institute 1n 2012 -2013. The seeds were hand harvested at three initial moisture contents including 18, 16, 14% using wet weight basis. The seed samples then were sealed in polythene bags and stored in conditioned storages and for 8 months. After that for seed priming, seeds were soaked in 20mg/L-1 (w/v) aerated solution of Spermine (Spm), Puterscine (Put), and Spermidine (Spd) and distilled water (W) for 16h at 20±2C°. After each treatment, seeds were dried to original moisture level in forced air and sealed in polythene bags, stored in a conditioned storage again for one month. Experimental units were arranged as a completely randomized design with four replications after harvesting time and one month conditioned storage after treatment. The result indicated that 18% MC improved the germination percentage and seedling vigour compared to 16 and 14 % MC. Also after 8 month storage, 18% Mc had the highest germination percentage and seedling vigour. The electrolyte leakage decreased in seeds with 18% MC compared to 16 and 14% MC. Also seed treatments significantly increased the seed viability and germination percentage, whereas, mean germination time and electrical conductivity were reduced in all seed treatments. Meanwhile, maximum germination percentage was detected for seed Spm seed treatment and 18% MC. Generally by decreasing moisture content of seed and delay in harvesting time, seedling vigour decreased and the optimum moisture content and seed treatment were 18% and Spm, respectively.
Volume 3, Issue 1 , March 2014, , Pages 31-40
Abstract
Seed priming improves some properties of seedling. The objective of this research was to evaluate the effect of salicylic acid and gibberellins on germination characteristics and changes of biochemically of sunflower seeds under accelerated aging, experimental design was factorial with complete randomized ...
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Seed priming improves some properties of seedling. The objective of this research was to evaluate the effect of salicylic acid and gibberellins on germination characteristics and changes of biochemically of sunflower seeds under accelerated aging, experimental design was factorial with complete randomized design as base design with 3 replications. The first factor was priming by salicylic acid and gibberellin, secondary factor was combinations 4 levels of aging (0, 2, 4 and 6 days of aging at 41°C). The results showed that the priming and aging effects on germination percentage, germination index, normal seedling percentage, seedling length, vigor index, abnormal seedling percentage, catalase and ascorbate peroxidase were significant, but on mean time to germination not significant. Results showed that, the highest germination characteristics were attained from priming by salicylic acid and gibberellins under non aged conditions. Also, seed priming increases enzyme activity.
Volume 2, Issue 2 , December 2013, , Pages 229-239
Abstract
In order to evaluate the effect oftemperature and bed drying methodon vigor of two cultivars ofsoybeanseeds Katoul (DPX) and Sari (JK), an experiment was performed at Agricultural Engineering Research Institute (AERI) and Seed and Plant Certification and Registration Research Institute (SPCRI) in Karaj. ...
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In order to evaluate the effect oftemperature and bed drying methodon vigor of two cultivars ofsoybeanseeds Katoul (DPX) and Sari (JK), an experiment was performed at Agricultural Engineering Research Institute (AERI) and Seed and Plant Certification and Registration Research Institute (SPCRI) in Karaj. Seeds with primary moisture 16-17% dried at four temperatures 30˚C, 40˚C, 50˚C and 60˚C and three beds (stable,threshold fluidizingand fluidized). Then percentage of normal seedling, length of seedling, seedling dry weight,seedling vigorindex, final seedling emergenceand leaf area index were assessed. Result showed that temperature and bed affected traits. The highest germination(normal seedling) percentage wasrelated to Sari (JK) at 40°C in the fixed bed.Result showed that length and dry weight of seedling in Katoul (DPX) was better than Sari (JK). Also higher leaf area index was observed in Katoul (DPX) at 30°Cin the threshold fluidized bed.According to the results, 30-40°C are suitable temperature for dryingseeds. Also constant bed in fluidized-bed drying was the best bed treatment.
