Parimah Shokouhi Nasab; Hamidreza Balouchi; َAli Moradi; Mohsen Movahhedi Dehnavi
Abstract
This experiment was conducted in order to investigate the effect of seed coating with biochar and activated carbon on some quinoa seed germination indicators, and it was implemented as a factorial in the form of a completely randomized design with four replications in 2019, in Yasouj University's Faculty ...
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This experiment was conducted in order to investigate the effect of seed coating with biochar and activated carbon on some quinoa seed germination indicators, and it was implemented as a factorial in the form of a completely randomized design with four replications in 2019, in Yasouj University's Faculty of Agriculture. The first factor is seed coating in four levels (no coating, coating with activated carbon, coating with biochar and coating with activated carbon + biochar) and the second factor includes salinity stress in four levels (zero, 75, 150 and 225 mM sodium chloride). The results of the interaction of salinity stress and seed coating showed that the highest content of soluble sugar (28.578 mg/g seed FW) and malondialdehyde (2.97 µmol/g seed FW) was obtained by coating with activated carbon in 150 mM salinity stress. Also, the highest amount of seed hydrogen peroxide (0.18 μmol/g seed FW) and proline (10.49 μmol/g seed FW) at the level of 225 mM sodium chloride was obtained by covering the seeds with biochar and active carbon, respectively. Covering seeds with activated carbon and biochar led to an increase in the length of root and shoot under salt stress conditions. However, under salinity stress conditions, root weight and length vigor index of seed improved more by coating seeds with activated carbon. It can be stated that seed coating can greatly reduce the harmful effects of osmotic stress on germination and biochemical traits in quinoa seedlings and improve seedling growth.
Sajad Argha; Hamidreza Balouchi; Ali Moradi; Alireza Yadavi
Abstract
This research was carried out to evaluate the storage of wheat seed which produced under different conditions of water deficit and application of chemical nitrogen and biological fertilizers as the first factor was water deficit in milky stage, drought stress at dough stage and without stress (control), ...
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This research was carried out to evaluate the storage of wheat seed which produced under different conditions of water deficit and application of chemical nitrogen and biological fertilizers as the first factor was water deficit in milky stage, drought stress at dough stage and without stress (control), and nitrogen fertilizer resources in Urea fertilizer 100%, 75%, 50% and 25% of plant requirement with nitroxin and nitragin. The second factor was storage conditions in 7 and 14% seed moisture within 20 and 4 °C storage, and the third factor was the storage period at 50, 100, 150 and 200 days. The results showed that the content of soluble protein and seed vigor index in seeds that received nitroxin and nitragin fertilizers with 25% urea, by increasing the water deficit, from 1.208 to 0.581 mg/g fresh weight and from 1.945 to 0.376 increased relative to 100% urea, respectively. The amount of catalase activity in seeds with drought stress and biofertilizer was higher and its activity increased at higher storage temperatures. In general, if the irrigation of the maternal plant is discontinued at the seed dough stage and the plant is fed with integrated of chemical and biological fertilizers, then the seed produced had more storage capability.
Ali Hoseinipoor; Alireza Yadavi; Hamidreza Balouchi; َAli Moradi
Abstract
Germination is one of the most sensitive stages of plant life, which is affected by various environmental factors such as temperature and humidity. Because seed standard germination test alone cannot accurately describe seedling emergence in field conditions, seed strain tests such as accelerated aging ...
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Germination is one of the most sensitive stages of plant life, which is affected by various environmental factors such as temperature and humidity. Because seed standard germination test alone cannot accurately describe seedling emergence in field conditions, seed strain tests such as accelerated aging test (seed deterioration) are used. In this research, germination and biochemical indices of linseed (Linum usitatissimum Var. Norman) in moisture potential of 0, -0.2, -0.4, -0.6, -0.8, -1.2 and -1.2 MPa under accelerated aging conditions at temperatures 10, 15, 20, 25, 30 and 35°c were evaluated. The results showed that the percentage and rate of germination and seedling vigor index in accelerated aging and non-accelerated aging respectively were increased by increasing temperature up to 20 and 25 ° C, , then decreasing to 35 ° C. And with decreasing potential water, in terms of accelerated aging, showed its lowest level at any temperature. The amount of soluble protein, catalase activity and proline content of seeds increased with decreasing water potential and accelerated aging effects at each temperature. Also, with increasing temperature, protein content and catalase enzyme activity of Linum usitatissimum seed are increased; proline content of the seed decreased.The results of this study showed that Linum usitatissimumis resistant to high and low temperatures in the early stages of germination under favorable temperature conditions of plants resistant to dehydration and under stress-free conditions.
atfeh hoseini; َAli Moradi; H.R. Balouchi
Abstract
In order to investigate seed bio-priming effects on some germination index of Pimpinella anisum L. under drought stress, this research was done in genetic lab at Agriculture department of Yasouj University. The experiment was factorial with two factor based on CRD design in 4 replications and in each ...
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In order to investigate seed bio-priming effects on some germination index of Pimpinella anisum L. under drought stress, this research was done in genetic lab at Agriculture department of Yasouj University. The experiment was factorial with two factor based on CRD design in 4 replications and in each Petri dishe 30 seeds were planted. First factor was seed bio-priming with nine level (on bio-priming, 4 Bactria strain (P.fluorescent), fluorescent (CHA0), 1) P.F (, 2) P.F (, 16) B (and 52 (B)) and 4 strain of T.harzianum (T29, T32, T43, T36 and T39) and second factor include osmotic potential of drought stress in 3 levels (0, -3 and -6 bar). The evaluated traits in this study included germination percentage, germination rate, seedling length, dry weight, seedling vigour index (weight and length) and alometric coefficient. Results of test showed that increasing drought stress, decreased seed germination indexes, and bio-priming couldn’t improve these indexes than non-priming seeds, therefore in each of drought stress potential, T36 has the maximum germination percentage (83.88), germination rate (4.7), seedling length vigor index (10), seedling dry weight (8.07), and seedling weight vigor index (15.25) and not primed seeds have the lowest amount of this traits. According to the results, it can be stated that the application of bio-priming treatment can moderately reduce the adverse effects of drought stress in the germination stage of pimpinella anisum L.
Hossein Reza Rouhi; َAli Moradi; Maryam Saman; Yasin Mohammadi; Alireza Shahbodaghlo
Abstract
In order to investigate the effect of sodium nitroprusside (NO donor) on improvement of aged pumpkin seeds under drought stress, a factorial experiment was carried out based on completely randomized design with three replications in Bu-Ali Sina University, Hamedan, Iran. Seed priming with different concentrations ...
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In order to investigate the effect of sodium nitroprusside (NO donor) on improvement of aged pumpkin seeds under drought stress, a factorial experiment was carried out based on completely randomized design with three replications in Bu-Ali Sina University, Hamedan, Iran. Seed priming with different concentrations of sodium nitroprusside including 0 (hydropriming), 25, 50 and 75 µM in drought stress levels (0, -0.2, -0.46 and -0.6 MPa) was performed. Germination percentage, germination rate, seed vigor, seedling length, soluble carbohydrates, soluble proteins, memberane electrical conductivity, malondialdehyde content and antioxidant enzymes activity (catalase, ascorbate peroxidase and superoxide dismutase) were evaluated. The results showed that seed priming with different concentrations of sodium nitroprusside mitigated significant decrease of germination parameters of aged seeds under drought stress. At -0.6 MPa, seed priming with 75 µM of sodium nitroprusside increased germination rate, vigor index , soluble carbohydrates and proteins 25, 214.1, 90.8, 117.1%, as well as the activity of catalase, superoxide dismutase and ascorbate peroxidase increased by 34, 14.5, 41.4% in comparison to control, respectively. Therefore, seed priming with sodium nitroprusside specialy with 75 µM is suggested for aged pumpkin seeds by decreasing oxidative stress due to drought stress.
T. Bazi; َAli Moradi
Volume 5, Issue 2 , January 2016, , Pages 143-155
Abstract
Prangos ferulacea L. is a plant from Apiaceae family which is used in medicinal and forage industries. Because of problems during germination and growth of this plant, several experiments were conducted in order to break dormancy and its germination enhancing. Seed samples were collected from their ...
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Prangos ferulacea L. is a plant from Apiaceae family which is used in medicinal and forage industries. Because of problems during germination and growth of this plant, several experiments were conducted in order to break dormancy and its germination enhancing. Seed samples were collected from their natural habitats (ridge of Vezg, Kogiluyeh and Boyerahmad province) in three dates of 7, 17 and 27 June 2012 and possibility of dormancy breaking were examined in the form of three separate experiments with three replicates. The first experiment factors consisted of three levels of collecting time (mentioned above) and GA3 in two levels (1000 and 1500 mg.l-1) which were applied along with 2 week’s moist chilling. The second experiment was consisted of collecting time at three levels along with moist chilling, that chilling was applied for four weeks. The third experiment factors were consisted of seed collection time in three levels and germination temperatures at two levels (15 and 20 ̊C). The Seeds were moist-chilled at 4°C for 8 weeks and then transferred to the incubators with the temperatures of 15°C and 20 °C. In the first experiment, germination of seeds collected in the third date and treated with GA 1500 mg.l-1 (33.6%) was higher than that of 1000 mg.l-1 (17.6%). The second study showed a significant positive effect of collecting time on seed germination of this plant, so that germination of seeds collected in the third date was almost doubled compared to the first date. The thirds experiment results showed that the temperature of 15 °C is more suitable than 20 °C for Prangos ferulacea seed germination. In all the three experiments the highest germination (33.26% in the first, 33.61 % in the second and 43.02% in the third experiments) were achieved when the seeds were collected at full maturity. Seeds germination obtained from 8 weeks (30.52%) chilling was higher than that of two (13.15%) and four weeks (27%). The overall results showed that Prangos ferulacea seeds have levels of physiological dormancy and seed maturity level at the time of collecting have significant effect on seed germination of this plant.
