S. Sheidaei; Aidin Hamidi; Hossein Sadeghi; Bita Oskouei
Abstract
A factorial experiment was conducted to evaluate the biochemical changes during the soybean seed deterioration based on a completely randomized design. The treatments consisted of germination in two levels of minimum and high quality, three initial seed moisture content (low, medium and high moisture), ...
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A factorial experiment was conducted to evaluate the biochemical changes during the soybean seed deterioration based on a completely randomized design. The treatments consisted of germination in two levels of minimum and high quality, three initial seed moisture content (low, medium and high moisture), and two storage conditions were considered including the common storage in Moghan and the controlled storage condition. Indices of germination percentage, vigor and biochemical changes including protein content, soluble sugars, malondialdehyde, catalase and peroxidase enzymes were evaluated. The study of biochemical changes of deteriorated seeds during the storage showed that, with the increase of seeds’ deterioration, the soluble sugars and protein percentage decreased, as well. Moreover, seeds’ storage in poor condition caused a significant decrease in seeds’ soluble sugar and total protein content. Also, the increase of seed moisture was followed by further seed deterioration that significantly decreased the content of soluble sugars and total protein. Significant differences were observed between seeds with different initial germinations and they had significantly higher content of soluble sugars and protein percentages. Furthermore, measuring the amount of malondialdehyde and the activity of catalase and peroxidase enzymes indicated that there exists a significant correlation between the degree of seed deterioration with the level of malondialdehyde and the activity of scavenging enzymes. Overall, along with the decrease of germination and the quality of soybean seeds after the storage, the amount of lipids peroxidation increased and the seed deterioration decreased the activity of catalase and peroxidase enzymes during the storage.
Bita Oskouei; Aidin Hamidi; Saman Sheidaei; maryam divsalar; hossein sadeghi
Abstract
This experiment was done in seed and plant certification and registration institute laboratory in 2015. The treatments were corn seed produced in Karaj and Moghan , seed storing condition in two controlled and Free weather conditions stores also Disinfection and non- Disinfection. The experiment was ...
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This experiment was done in seed and plant certification and registration institute laboratory in 2015. The treatments were corn seed produced in Karaj and Moghan , seed storing condition in two controlled and Free weather conditions stores also Disinfection and non- Disinfection. The experiment was conducted as factorial based on completely randomized design in three replications. Germination percent of Karaj seeds before accelerated aging test was 92 percent and after 144 hours aging, it reduced 3 percent in controlled storage and it dropped 6 percent in Moghan conditions storage. While similar condition led to a decrease of 18 and 28 percent in Moghan seeds produced. When seeds were stored in controlled storage, indicated 17 percent higher protein content compared to storage in Moghan conditions. Catalase activity of Karaj seeds was higher than Moghan seeds (14%). Seed treatment had significant effect on peroxidase and MDA content, as peroxidase activity in treated seeds was 11 percent higher and MDA content was 8 percent lower than untreated seeds. Karaj seeds in controlled storage had the highest peroxidase activity and the lowest MDA content. So production seed conditions in Moghan was more critical in terms of temperature and humidity than Karaj so the biochemical properties of seeds have been affected. So that Moghan seeds had less storability after storing. On the other hand, seeds in Moghan store subjected to more critical temperature and humidity than controlled condition so more deteriorated.
A. Hamidi; Hossein Sadeghi; Hamidreza Gazor; Saman Sheidaei; Bita Oskouei; Hassan Mivechi; Mohammad Nouri; Shapour Alizadeh; Saber Seifamiri; Leila Zare; Azam Dashti
Abstract
This research in order to study on post-harvest process effect on 2 soybean commercial Williams and Saba (L17) in Moghan region was conducted as a 2×5, two cultivars× 5 post-harvest process stages including after harvest, after drying, after cleaning after packing and stacking and storage, ...
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This research in order to study on post-harvest process effect on 2 soybean commercial Williams and Saba (L17) in Moghan region was conducted as a 2×5, two cultivars× 5 post-harvest process stages including after harvest, after drying, after cleaning after packing and stacking and storage, factorial experiment based on completely randomized design by 3 replications. Studied traits were normal seedlings percent in standard germination test and after accelerated ageing test, seedling weight vigour index after standard germination test, broken seeds percent, cracked coat seeds percent and electrical conductivity. Results revealed Williams cultivar had more normal seedlings percent after accelerated ageing test and seedling weight vigour index after standard germination test and less cracked coat seeds percent. Also the least normal seedlings percent in standard germination test, normal seedlings percent after accelerated ageing test and seedling weight vigour index after standard germination test and the most broken seeds percent, cracked coat seeds percent and electrical conductivity investigated. Based on this research results Williams cultivar seeds had more germination ability and vigour preservation potential during post-harvest process stages than Saba (L17) cultivar. Also germination ability and vigour decrease after drying and storage stages revealed seed drying unsuitability.
