Haniyeh Saadat; Mohammad Sedghi
Abstract
In order to investigate the effect of hydropriming and hormone priming on some physiological and biochemical characteristics in chickpea under salinity stress a factorial experiment was conducted based on completely randomized design at the University of Mohaghegh Ardabili in 2023 with 3 replications. ...
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In order to investigate the effect of hydropriming and hormone priming on some physiological and biochemical characteristics in chickpea under salinity stress a factorial experiment was conducted based on completely randomized design at the University of Mohaghegh Ardabili in 2023 with 3 replications. treatments included four salinity levels (0, 50, 75 and 100 mM) and four levels of priming (control, hydropriming, priming with gibberellin (50 ppm) and salicylic acid (100 ppm)). The results showed that salinity stress reduced germination percentage (GP), germination rate (GR), radicle and Seedling length (RL and SL) But, seed priming improved these traits. Salinity increased the allometric coefficient (AC), So that the highest was observed at (0.860) salinity of 100 Mm. The highest mean daily germination (MDG), plumule length (PL) and total seed protein content were observed in priming with gibberellin and control (without salinity). The maximum DGS (0.144) and MDA (0.0137 mmol/g FW) were related to the treatment with without priming and salinity of 100 mM. The amount of soluble sugars in gibberellin treatment and salinity of 100 mM compared to the control showed an increase about 56%. Also, the use of gibberellin increased amount of proline by about 40% compared to the control (without priming). In general, seed hydropriming, salicylic acid, especially gibberellin
Iman Nemati; Mohammad Sedghi; Ghasem Hoseini Salekdeh; Reza Tavakkol afshari; Mohammadreza Naghavi
Abstract
Common cocklebur (Xanthium strumarium L.) is a widespread weed with high medical value and interested for researchers because of deep dormancy in one of its two seeds in one bur. However, lack of genomic data has led to low information about it. Transcriptome large and small seeds were sequenced using ...
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Common cocklebur (Xanthium strumarium L.) is a widespread weed with high medical value and interested for researchers because of deep dormancy in one of its two seeds in one bur. However, lack of genomic data has led to low information about it. Transcriptome large and small seeds were sequenced using Illumina platform to identify and functional analysis of differentially expressed transcripts in two seeds. Identified sequences in each seeds were compared and differentially expressed genes were functionally annotated. In this research 191192 sequence with a mean of 989.69 bp were detected. Sequence similarity analysis and functional analysis was carried out aginst nr, GO and KEGG databases. Differentially expressed genes had the most similarity with sunflower (83.41 per cent) in terms of top hits. GO analysis led to identify 615 functional annotation distributed in 36 categories. The most abomdant GO in biological process was biosynthesis. Results of our research shows the higher biosynthetic and metabolic processes in large seed of certain bur rather than the small one and also key regulatory role of transcription during seed development.
