Nasrin Teimoori; Mohsen Saeidi; Mahmoud Khoramivafa; Shahab Khoshkhoi
Abstract
Seed priming has been introduced as an effective and practical mechanism for increasing seed germination resistance and creating more vigorous seedlings against abiotic stresses such as drought and salinity. Therefore, this research was carried out as a factorial experiment to investigate the effect ...
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Seed priming has been introduced as an effective and practical mechanism for increasing seed germination resistance and creating more vigorous seedlings against abiotic stresses such as drought and salinity. Therefore, this research was carried out as a factorial experiment to investigate the effect of priming chickpea seeds with iron oxide nanoparticle and bulk forms on the germination characteristics of chickpea cultivar Adel under salinity stress. The test factors include 1) seed priming with iron oxide bulk and nanoparticles 1-100 nm and 40-60 nm in three concentrations of 2, 4, 8 mg l-1, hydro-prime and non-prime, and 2) salinity stress (30, 60, 90 mM of sodium chloride). he results showed that the salinity of 90 mM completely stopped the germination. Levels of 30- and 60-mM salinity caused a 23 and 63% decrease in germination and 72 and 89% decrease in germination rate, respectively. Prime treatments significantly increased germination percentage and rate, mean daily germination, seed vigor, and allometric coefficient, and significantly decreased abnormal seedlings, especially at 60 mM of salinity. Salinity stress was observed to affect the germination rate more than the germination percentage. The most effective seed priming treatments were iron oxide bulk and nanoparticles 40-60 nm, 8 mg l-1. Interestingly, the iron oxide nanoparticles showed no significant advantage over the bulk iron oxide. These findings suggest that priming treatments can enhance the salt resistance of chickpeas during the germination stage.
Nasrin Teimoori; Mohsen Saeidi; Mahmood Khoramivafa; Shahab Khoshkhoi
Abstract
The utilization of nanoparticles for seed priming may enhance the germination and seedling growth compared to bulk forms. Therefore, this research aims to investigate the effect of seed priming (with bulk iron oxide, nanoparticles of 1-100 nm and 40-60 nm in concentrations of zero, 2, 4, and 8 g l-1, ...
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The utilization of nanoparticles for seed priming may enhance the germination and seedling growth compared to bulk forms. Therefore, this research aims to investigate the effect of seed priming (with bulk iron oxide, nanoparticles of 1-100 nm and 40-60 nm in concentrations of zero, 2, 4, and 8 g l-1, water, and no priming) at different levels of drought stress (including no stress, -2, -4 and -8 bar) using polyethylene glycol 6000 on the characteristics of germination and growth of Adel chickpea cultivar. This research was performed as a factorial experiment based on a completely randomized design in three replications at Razi University. The results showed that germination ceased completely under drought stress of -8 bar. Drought stress at -4 bar after -8 bar resulted in a significant decrease in the percentage, speed, and vigor of germination by 96, 93, and 40%, respectively, and 130% increase in root length/shoot length ratio compared to the non-drought stress treatment. Seed priming treatments had the most positive and significant effect on the speed and vigor of germination in non-drought stress treatment and on the germination percentage in drought stress treatment. The seed priming with iron 8 g l-1 and 40-60 nm produced the highest length and weight vigor of germination. It appears that the priming treatments have resulted in better vigor and rate of germination in non-drought stress condition while germination percentage in drought stress. The seed priming treatment using iron 40-60 nm is the most effective compared to other priming treatments.
