Parisa Ahmadi; Mohammed Hossein Gharineh
Abstract
To evaluate different priming treatments under salinity stress conditions, an experiment was conducted in a factorial design based on completely randomized design with four replications in the Seed Technology Laboratory of the Department of Plant Production and Genetics Engineering at Agricultural Sciences ...
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To evaluate different priming treatments under salinity stress conditions, an experiment was conducted in a factorial design based on completely randomized design with four replications in the Seed Technology Laboratory of the Department of Plant Production and Genetics Engineering at Agricultural Sciences and Natural Resources University of Khuzestan in 2022. The first factor included different salinity levels (0, 50, 100, 150, and 200 mM), the second factor included priming treatments (control (no priming), gibberellic acid, and potassium nitrate), and the third factor included temperature (10 and 25 C). The results showed that at 25 C, germination completely stopped at salinity levels above 100 mM in all priming treatments (gibberellic acid, potassium nitrate, and no priming). However, at 10 C and a salinity level of 200 mM, 93% of the seeds were able to germinate under gibberellic acid and potassium nitrate priming conditions. The germination percentage of black cumin seeds at 25 C and a salinity level of 50 mM was 54% and 48% under gibberellic acid and potassium nitrate priming conditions, respectively, showing a significant difference compared to the control treatment (32%). Under gibberellic acid application at 25 C, the highest mean germination time (11 days) was observed. Overall, considering salinity stress and the cultivation of medicinal plants, the application of gibberellic acid and potassium nitrate is recommended to achieve optimal germination and subsequent establishment of black cumin seedlings.
Saeed Saeedipour
Abstract
This experiment was conducted in order to evaluate the effects of osmo-priming on the characteristics of germination, seedling and some physiological characteristics of two cultivars of rapeseed (Brassica napus L.) under saline conditions. The seeds of two spring rape varieties were pretreated with 29.5 ...
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This experiment was conducted in order to evaluate the effects of osmo-priming on the characteristics of germination, seedling and some physiological characteristics of two cultivars of rapeseed (Brassica napus L.) under saline conditions. The seeds of two spring rape varieties were pretreated with 29.5 mM potassium nitrate (KNO3) for 12 hours at 30 degrees Celsius. In the first experiment after priming, 25 primed and unprimed seeds were placed in petri dishes with 4 levels of salinity (0, 50, 100 and 150 mM of NaCl) in a completely randomized design in four replications. The results showed that the germination percentage of primed seeds was higher than non-primed seeds.In the second experiment, 20 primed and unprimed seeds were planted in pots filled with perlite and peat at a ratio of 3:1. The pots were kept for three weeks in the greenhouse with a temperature of 26/16 degrees Celsius . The pots were watered every day with saline solutions (0, 50, 100 and 150 mM of NaCl). The results of multi-way interaction also showed that, primed seeds had higher antioxidant enzyme activity and higher content of soluble sugars and proline, at the same time, cell membrane damage in them was lower due to lower concentration of malondialdehyde compared to non-primed seeds. Under salinity conditions of 150 mM, the highest dry weight, antioxidant activity, and accumulation of soluble sugars and lower concentration of malondialdehyde were observed in Hyola 401 variety. This research showed that priming with KNO3 improves canola seed germination under stress conditions.
Bita Oskouei; Abbas Dehshiri; Hadis Afshar; Elahe Gheysari; Ali Shayanfar
Abstract
An experiment was implemented based on completely randomized design with four replications to determine standard Flixweed (Descurainia sophia) germination test condition. Flixweed seeds were placed in germinator for 14 days under 6 temperatures 10, 15, 20, 25, 20-30, 35ºC exposed to three treatments ...
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An experiment was implemented based on completely randomized design with four replications to determine standard Flixweed (Descurainia sophia) germination test condition. Flixweed seeds were placed in germinator for 14 days under 6 temperatures 10, 15, 20, 25, 20-30, 35ºC exposed to three treatments of potassium nitrate (2 grams per 1000 ml of distilled water), pre-chilling (7-10ºC for 7 days), pre-soaking (24h in distilled water), two cellulose substrates of top paper and between paper, and sands. Then, germination indexes were assessed. After comparison of three mentioned treatments (potassium nitrate, pre-chilling, and pre-soaking) with control it was demonstrated that the highest germination rate belonged to pre-chilling in 15ºC.The lowest amount of germination indices and the highest percentage of dead seeds were found in 25 and 35ºC. The best treatment to improve germination and seedling vigor was applying of potassium nitrate and optimal temperatures to reach maximum of germination was 10 and 15ºC. Higher germination was achieved when top and between paper substrate were used than sand. In total, it was suggested that it would be better for potassium nitrate to be under 10 and 15ºC when top and between paper and sand were applied, respectively to optimal condition for standard germination determination was achieved, because the highest percentage of final germination, normal seedlings, and seedling weight vigor, and also the lowest percentage of dead seeds were observed.
F. Sharifzadeh; Arezoo Espanany; Mohammad Reza Naghavi
Abstract
In this research, the effect of different seed priming treatments was investigated on seed germination parameters of Securigera securidaca seeds in the low temperature stress conditions. Seeds first were exposed to eight temperature levels (5, 10, 15, 20, 25, 30, 35 and 40 ° C). Experimental treatments ...
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In this research, the effect of different seed priming treatments was investigated on seed germination parameters of Securigera securidaca seeds in the low temperature stress conditions. Seeds first were exposed to eight temperature levels (5, 10, 15, 20, 25, 30, 35 and 40 ° C). Experimental treatments included different priming treatments (hydropriming, osmopriming including potassium nitrate and calcium chloride and no priming). The results showed that temperature significantly reduced germination percentage and rate, seedling length and seedling dry weight, normal seedling percentage and seed vigor. 5 mM of calcium chloride treatment at 10 ° C for 20 hours had the highest germination percentage, and 1% of potassium nitrate treatment at 10 ° C for 20 hours had the maximum seedling dry weight and 0.5% of potassium nitrate at 10 ° C for 20 hours and 10 mM of calcium chloride treatments at 15 ° C for 20 hours had the highest percentage of normal seedlings. Hydropriming treatment at 15 ° C for 20 hours had the highest germination rate, seedling length and seed vigor and was also one of the best treatments in terms of normal seedling percentage. So this treatment can be introduced as a suitable treatment for this plant because it is better in most germination traits and it is easier and cheaper to do.
Elham Harati; Seyyed Gholamreza Moosavi; Fatemeh Nakhaei; Mohamad Javad Seghatoleslami
Abstract
Seed germination is the first and most sensitive developmental stage in the plant life cycle and the key process in seedling emergence. On the other hand, one of the major barriers to optimum use of medicinal plants outside the natural habitat is limited level of seed germination and seed dormancy. Hence ...
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Seed germination is the first and most sensitive developmental stage in the plant life cycle and the key process in seedling emergence. On the other hand, one of the major barriers to optimum use of medicinal plants outside the natural habitat is limited level of seed germination and seed dormancy. Hence in order to find the best priming and temperature treatment to dormancy breaking and seed germination of medicinal plant seed Pulicaria gnaphalodes (Vent.) Boiss a factorial experiment based on the completely randomized design with four replications and two factors was conducted in Islamic Azad University, Birjand branch in 2017. The first factor of priming type is 11 levels included in potassium nitrate 2% treatment at two times of 24 and 48 hours, gibberellic acid (0, 250 and 500 mg/ l) at two times of 24 and 48 hours, and stratification (7, 14 and 21 days). The second factor was temperature at three levels (15, 20 and 25 °c). The results showed that highest germination rate and percent germination were related to stratification at 7 and 21 days at 25 °c and maximum seedling length, plumule length, radicle length, radicle/plumule length ratio, wet weight of seedling, dry weight of seedling, percent germination, germination rate, longitudinal index seed vigor, weight index seed vigor related to 21 days wet temperature and 15°c. Therefore, 21 days stratification and temperatures of 15 and 25 °c were the best priming and temperature treatments to dormancy breaking and seed germination of medicinal plant seed Pulicaria gnaphalodes.
Sayyed Saeed Moosavi; Payman Nosrati; Mohammad Reza Abdollahi
Abstract
Datura is an important medicinal plant for production of various alkaloids. Seed dormancy and the problem of germination is the most important barrier to produce this plant. In order to solve the germination problem in this important medicinal plant, the present study was carried out as a factorial experiment ...
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Datura is an important medicinal plant for production of various alkaloids. Seed dormancy and the problem of germination is the most important barrier to produce this plant. In order to solve the germination problem in this important medicinal plant, the present study was carried out as a factorial experiment with three factors based on completely randomized design with three replications. The first factor consisted of two datura ecotypes (Hamadan and Esfahan), the second factor included three levels of physical treatments of breaking dormancy (no scratching; scratch with sandpaper and wet bathing of bain marie bath for 7 days at 60 °C; scratch with sandpaper and dry chilling for 7 days at 4 °C), and the third factor included five levels of chemical treatments of breaking dormancy (no application of chemical treatments [putting the seeds on distil water from the beginning of the experiment to the end of the experiment]; NaOH 20% for 90 minutes; NaOH 20% for 90 minutes + gibberellin 500 ppm for 48 hours; NaOH 20% for 90 minutes + potassium nitrate 300 ppm [putting the seeds in potassium nitrate solution from the beginning of the experiment to the end of the experiment]; NaOH 20% for 90 minutes + gibberellin 500 ppm for 48 hours + potassium nitrate 300 ppm). Interaction of ecotypes and seed dormancy breaking treatments was significant for most traits except; number of leaves per plant, root fresh weight, shoot length and stem fresh weight. The results showed that the chemical treatment of NaOH 20% + gibberellin 500 ppm, was the best dormancy braking treatment for the fresh harvested seeds of datura. Therefore, using this treatment is recommended to break seed dormancy more than 70 percent, without significant damage on the seeds. In general, it was found that the chemical treatments were more effective than physical treatment on breaking the dormancy of datura seed and the main cause of breaking seed dormancy and stimulation of vegetative growth of seedlings obtained was the combination of NaOH with gibberellin.
M. Jamali; M.R. Jahansuz; Reza Tavakkol afshari; J. Asghari
Abstract
To study the effects of tillage systems and seed priming on yield and yield components of three soybean cultivars, a field experiment as split factorial structure based on a randomized complete block design with three replications was carried out at Research Field of Faculty of Agriculture, University ...
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To study the effects of tillage systems and seed priming on yield and yield components of three soybean cultivars, a field experiment as split factorial structure based on a randomized complete block design with three replications was carried out at Research Field of Faculty of Agriculture, University of Tehran, Karaj, Iran in 2014. The main plots were two tillage systems level (conventional and No-till) and factorial combinations of seed priming at two levels (priming with 0.4 of Potassium nitrate and No-priming) and three soybean genotypes (Williams, L17 and Nekador) were assigned in sub-plots. Results showed that tillage system × priming interaction was significant (p ≤0.01) seedling establishment. The highest value of plant density (25.3 plants per meter square) was related to non-priming in conservation tillage system. Also, seed priming reduce grain yield in both conventional and conservation tillage systems. Results showed that grain yield significantly decreased by seed priming (3.09 and 1.39 kg ha-1 in non-prime and primed seeds, respectively). In the most traits, except number of grain per pod, plant height and harvest index, Williams cultivar was better than L17 and Nekador cultivars. Since grain filling period of Nekador cultivar was occured at cold tempratures, this cultivar couldn’t produce its potential yield. The highst value of grain yield (3559 kg.ha-1) was related to Williams cultivar in conventional tillage system. Also, in conservation tillage system, the highst value of grain yield was attained in L17 cultivars that don’t showed significant difference with other cultivars. In conservation tillage systems, yield losses by soil compaction can be compensated by higher seeding density
M. Khajeh-Hosseini; M. Alinaghizadeh; S.A. Hosseini; M.H. Rashed Mohasel
Volume 5, Issue 2 , January 2016, , Pages 199-213
Abstract
In order to study the seed germination and dormancy of 3 weed species (Prosopis farcta, Launaea acanthodes and Cressa cretica) of pistachio orchards in Rafsanjan, Iran, three factorial experiments were conducted based on completely randomized design with four replications, at the faculty of agriculture, ...
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In order to study the seed germination and dormancy of 3 weed species (Prosopis farcta, Launaea acanthodes and Cressa cretica) of pistachio orchards in Rafsanjan, Iran, three factorial experiments were conducted based on completely randomized design with four replications, at the faculty of agriculture, Vali-E-Asr university of Rafsanjan, Iran, in 2014. Weed seeds were collected from five different regions of Rafsanjan, including Markazi, Anar, Koshkoiyeh and Nogh. Dormancy breaking treatments for P. farcta included scarification by scalpel and sandpaper, chemical scarification by sulfuric acid (30 and 40 min), and distilled water as control. Treatments for L. acanthodes included gibbereilic acid (250, 500 and 1000 ppm), KNO3 (500 and 1000 ppm), cold stratification (for 1, 3 and 5 weeks). Treatments for C. cretica included scarification by scalpel and sandpaper, sulfuric acid (5 and 10 minute), boiling water (for 15 and 30 min) and distilled water as control. Results showed that seed germination percentage (SGP) and mean germination time (MGT) was significantly different among different weed populations and dormancy breaking methods. Seed scarification by scalpel and sulfuric acid resulted in highest SGP in P. farcta and C. cretica. For all L. acanthodes populations, cold stratification for 5 weeks increased SGP, especially in Anar (94 %). Using KNO3 at 500 ppm, and 250 ppm gibbereilic acid, significantly increased germination percentage of L. acanthodes seeds (79 and 74.8 %, respectively), but higher concentration of KNO3 and gibbereilic acid, reduced germination percentage.
Volume 3, Issue 1 , March 2014, , Pages 97-107
Abstract
In order to evaluate the effect of different seed priming treatments on germination parameters improvement in Pisumsativum L.a factorial experiment was conducted in completely randomized design with four replications in seed science and technology laboratory of agriculture faculty, Shahrekord University ...
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In order to evaluate the effect of different seed priming treatments on germination parameters improvement in Pisumsativum L.a factorial experiment was conducted in completely randomized design with four replications in seed science and technology laboratory of agriculture faculty, Shahrekord University in 2013. The experimental factors were consisted as priming types (osmopriming, halopriming and hydropriming) and priming times (12, 24 and 36 hours). Results showedthat different priming treatments had significant effect on radicle length, shoot length, shoot dry weight, radicle dry weight, germination percentage, germination rate, relative germination, vigor indexesIn addition to,priming times had significant effect in all measured traits except germination rate Themaximum radicle length, germination percentage, relative germination and vigor index I achieved with concentration of 1.1 MPa PEG and 24 hours. The maximum shoot length and germination rate obtained with distilled water treatment, 24 hours and concentration of 0.7 MPa PEG, 24 hours, respectively. The lowest amount of all measured traits was observed in concentration 1.2 MPa KNO3 with 36 hours. In conclusion, the high doses of PEGwith 24 hoursis suitable for germination improvement of pea seeds.
