Mahmood Abadeh; Malihe Sadeghi Bahmani; hamed hasanzadeh khankahdani; Maryam yektankhodaei
Abstract
This experiment was conducted as a factorial based on a completely randomized design with four replications and the first factor was Culture medium with two levels: laboratory and Greenhouse, and the second factor was seed pre-treatment with five levels: Control, Leaching for 12 hours, Leaching for 12 ...
Read More
This experiment was conducted as a factorial based on a completely randomized design with four replications and the first factor was Culture medium with two levels: laboratory and Greenhouse, and the second factor was seed pre-treatment with five levels: Control, Leaching for 12 hours, Leaching for 12 hours and Soaking in gibberellic acid for 12 hours, Scarification with sandpaper and Scarification with sandpaper and Soaking in gibberellic acid for 12 hours. The results showed that in Greenhouse vegetative traits of plumule length (78.6 mm), radicle length (109.34 mm), seedling dry weight (102.7 mg), allometric coefficient (0.72) and seedling vigour (95) were higher than the laboratory (p<0.05). The results also showed that leaching and soaking in gibberellic acid pre-treatment increased indices and traits germination rate (5.56), mean daily germination (1.9), peak value (2.48), germination value (6.1), plumule length (61.9 mm), radicle length (86.1 mm), seedling dry weight (82.2 mg) and seedling vigour (94.3) (p<0.05). This pre-treatment also reduced day to beginning germination index (5.6) (p<0.05). The results of mean comparison of the interaction effect of Culture medium and Pre-treatment showed that in Greenhouse leaching and soaking in gibberellic acid pre-treatment reduced day to beginning germination index (4.2) and increased vegetative traits of plumule length (93 mm), radicle length (129.2mm), seedling dry weight (120.8 mg) and seedling vigour (124.9) (p<0.05).
Abstract
In order to investigate the effect of seed bio-inoculation on some germination indices of Cumin under drought stress a two factors experiment was laid out. Experimental factors included priming in nine levels (bio- inoculation with 4 strains of PF1, PF2, PF75 and CHA0 of Pseudomonas fluorescent, and ...
Read More
In order to investigate the effect of seed bio-inoculation on some germination indices of Cumin under drought stress a two factors experiment was laid out. Experimental factors included priming in nine levels (bio- inoculation with 4 strains of PF1, PF2, PF75 and CHA0 of Pseudomonas fluorescent, and 4 strains T29, T36, T39 and T40 of Trichoderma harzianum and non-primed (control)) and three levels of drought stress (0, -3 and -6 bar imposed by PEG 6000). Results showed that by increasing the osmotic potential levels germination indices decreased. At all levels of stress, inoculated seeds had higher germination percentage and root length than non-inoculated seeds. In under stress level of -3 bar, highest germination percentage (86.66%) and seedling weight vigor index (11.93) was obtained from T36 strain that; this treatment was able increase germination by more than 35% than non-inoculated seed. Under -6 bar, the highest (76.68%) and lowest (55%) germination percentage was related to T29 that has no significant difference with T36 (75.83%) and T39 (75.83%) and the lowest amount was related to non-primed treatment (55%). Also at all stress level, the highest length seedling vigor index was related to CHA0 strain. The highest weight seedling vigor index was related to T36 strain (6.4, 5.49 and 4.7 respectively in 0, -3 and -6 abr) and the lowest (4.89) was related to non-inoculated (control) seeds. According to the results, T36 can be suggested as a suitable pretreatment in moderation of the negative effects of osmotic potential on germination of cumin seeds.
Arash Mohammadzadeh; Naser Majnon hoseini; Sadegh Asadi; Hossein Moghadam; mohsen jamali
Abstract
To study the effects of artificial seed ageing on germination indices in the laboratory condition and seedling establishment and yield of two red kidney bean genotypes in the field condition, an experiment was carried out at Research Field of Faculty of Agriculture, University of Tehran, Karaj, Iran ...
Read More
To study the effects of artificial seed ageing on germination indices in the laboratory condition and seedling establishment and yield of two red kidney bean genotypes in the field condition, an experiment was carried out at Research Field of Faculty of Agriculture, University of Tehran, Karaj, Iran in 2011. The two experiment design, were as factorial with four replications arranged in randomized complete blocks design (RCBD) and complete randomized design (CRD) for field and laboratory condition, respectively. The experimental treatments consisted of four levels of seed ageing – 0 (control), 2, 4 and 6 days seed ageing – and two red bean genotypes (Akhtar and Sayyad). Results showed that germination percentage, germination rate, normal seedling and seedling vigor index significantly decreased by seed ageing treatment in standard germination test. Also, seed ageing significantly decreased germination percentage, germination rate, seedling length and dry weight, normal seedling and seedling vigor index in accelerated ageing test. Studied genotypes was different in terms of germination percentage, germination rate, normal seedling, seedling length, seedling vigor index and electrical conductivity test. In the electrical conductivity test, seed leakage increased by increment of seed ageing level and is sever in Akhtar compared to Sayyad. In the field condition, seedling emergence and grain yield significantly affected by genotype and seed ageing. Seedling emergence index, seedling establishment percentage and grain yield decreased by seed ageing treatment that was severe in Akhtar than that of Sayyad genotype.
M. Soltani Kazemi; S. Abdanan Mehdizadeh; M.H. Gharineh
Abstract
The aim of this researchwas to investigate the possibility of using image processing to determine the quality of seed germination after applying dynamic loads. For this purpose, mechanical damage at four levels (0.0404, 0.0808, 0.1212 and 0.1617) on three cultivars of chickpea seed (Azad, Hashem and ...
Read More
The aim of this researchwas to investigate the possibility of using image processing to determine the quality of seed germination after applying dynamic loads. For this purpose, mechanical damage at four levels (0.0404, 0.0808, 0.1212 and 0.1617) on three cultivars of chickpea seed (Azad, Hashem and ILC) were investigated. The study was conducted based on a completely randomized factorial design. The results showed that the ILC had the lowest electrical conductivity (12.02), the highest germination rate (10.31), the highest seedling growth (18.69), the most vigor (84.2), the maximum radicle length (13.29), radicle dry weight (0.574), hypocotyle dry weight (0.271) and the highest seedling dry weight (0.844) among other cultivars. Mean comparison of energy treatment showed that with inceasing energy level, germination quality and vigor were reduced. Then, length and area of radicle were evaluated using image processing. Based on statistical analysis, there was no significant difference between image processing and manual measurments. This shows the potential of replacement of time-consuming manual with image processing method for examination of seedling parameters
T. Bazi; َAli Moradi
Volume 5, Issue 2 , January 2016, , Pages 143-155
Abstract
Prangos ferulacea L. is a plant from Apiaceae family which is used in medicinal and forage industries. Because of problems during germination and growth of this plant, several experiments were conducted in order to break dormancy and its germination enhancing. Seed samples were collected from their ...
Read More
Prangos ferulacea L. is a plant from Apiaceae family which is used in medicinal and forage industries. Because of problems during germination and growth of this plant, several experiments were conducted in order to break dormancy and its germination enhancing. Seed samples were collected from their natural habitats (ridge of Vezg, Kogiluyeh and Boyerahmad province) in three dates of 7, 17 and 27 June 2012 and possibility of dormancy breaking were examined in the form of three separate experiments with three replicates. The first experiment factors consisted of three levels of collecting time (mentioned above) and GA3 in two levels (1000 and 1500 mg.l-1) which were applied along with 2 week’s moist chilling. The second experiment was consisted of collecting time at three levels along with moist chilling, that chilling was applied for four weeks. The third experiment factors were consisted of seed collection time in three levels and germination temperatures at two levels (15 and 20 ̊C). The Seeds were moist-chilled at 4°C for 8 weeks and then transferred to the incubators with the temperatures of 15°C and 20 °C. In the first experiment, germination of seeds collected in the third date and treated with GA 1500 mg.l-1 (33.6%) was higher than that of 1000 mg.l-1 (17.6%). The second study showed a significant positive effect of collecting time on seed germination of this plant, so that germination of seeds collected in the third date was almost doubled compared to the first date. The thirds experiment results showed that the temperature of 15 °C is more suitable than 20 °C for Prangos ferulacea seed germination. In all the three experiments the highest germination (33.26% in the first, 33.61 % in the second and 43.02% in the third experiments) were achieved when the seeds were collected at full maturity. Seeds germination obtained from 8 weeks (30.52%) chilling was higher than that of two (13.15%) and four weeks (27%). The overall results showed that Prangos ferulacea seeds have levels of physiological dormancy and seed maturity level at the time of collecting have significant effect on seed germination of this plant.
Volume 4, Issue 1 , March 2015, , Pages 83-96
Abstract
This experiment was conducted as split factorial in a Randomized Complete Block Design (RCBD) with three replications in the research farm of Shahrekord University during 2010-2011 growing season. In this experiment irrigation treatments in three levels of: non water stress (irrigation after 50 mm evaporation ...
Read More
This experiment was conducted as split factorial in a Randomized Complete Block Design (RCBD) with three replications in the research farm of Shahrekord University during 2010-2011 growing season. In this experiment irrigation treatments in three levels of: non water stress (irrigation after 50 mm evaporation from class A pan), moderate and sever water stress (irrigation after 70 and 90 mm evaporation from class A pan, respectively) were used as main factor, while factorial combination of Zn (spraying with pure water, spraying of 100 and 200 g Zn per hectare) and Mn (spraying with pure water, spraying of 150 and 300 g Mn per hectare) were used as sub-factor. The results showed that drought stress did not have any significant effect on germination rate, mean germination time, seedling length and dry weight while significant increases were obtained on germination percentage, coefficient of velocity of germination and seed vigor indices (I and II). Moreover, Mn foliar application in amount of 150 g per hectare had the highest germination percentage, germination rate, coefficient of velocity of germination,vigor indices (I and II) and the lowest mean germination time. The highest germination rate, seedling length and dry weight and vigor indices (I and II) was obtained for 100 g Zn per hectare
