Haniyeh Saadat; Mohammad Sedghi
Abstract
In order to investigate the effect of hydropriming and hormone priming on some physiological and biochemical characteristics in chickpea under salinity stress a factorial experiment was conducted based on completely randomized design at the University of Mohaghegh Ardabili in 2023 with 3 replications. ...
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In order to investigate the effect of hydropriming and hormone priming on some physiological and biochemical characteristics in chickpea under salinity stress a factorial experiment was conducted based on completely randomized design at the University of Mohaghegh Ardabili in 2023 with 3 replications. treatments included four salinity levels (0, 50, 75 and 100 mM) and four levels of priming (control, hydropriming, priming with gibberellin (50 ppm) and salicylic acid (100 ppm)). The results showed that salinity stress reduced germination percentage (GP), germination rate (GR), radicle and Seedling length (RL and SL) But, seed priming improved these traits. Salinity increased the allometric coefficient (AC), So that the highest was observed at (0.860) salinity of 100 Mm. The highest mean daily germination (MDG), plumule length (PL) and total seed protein content were observed in priming with gibberellin and control (without salinity). The maximum DGS (0.144) and MDA (0.0137 mmol/g FW) were related to the treatment with without priming and salinity of 100 mM. The amount of soluble sugars in gibberellin treatment and salinity of 100 mM compared to the control showed an increase about 56%. Also, the use of gibberellin increased amount of proline by about 40% compared to the control (without priming). In general, seed hydropriming, salicylic acid, especially gibberellin
Nesa Gharehbaghli
Abstract
With the aim of evaluating the effect of salicylic acid in improving the efficiency of the aged pumpkin seeds under drought stress conditions, a factorial experiment was carried out in the form of a completely randomized design with three replications. Pretreatment of different concentrations of salicylic ...
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With the aim of evaluating the effect of salicylic acid in improving the efficiency of the aged pumpkin seeds under drought stress conditions, a factorial experiment was carried out in the form of a completely randomized design with three replications. Pretreatment of different concentrations of salicylic acid including zero (pretreated with water), 1 and 1.5 mM at different drought levels of zero, -0.2, -0.4 and -0.6 MPa were investigated. Indicators of mean germination time, germination percentage, seedling length, seed vigor, membrane electrolyte leakage, soluble carbohydrates, soluble proteins, malondialdehyde, ascorbate peroxidase activity, superoxide dismutase and catalase enzymes were evaluated. The results showed that the pretreatment of seeds with different concentrations of salicylic acid prevented the significant decrease in the germination indices of the deteriorated pumpkin seeds under drought stress. So that at the potential of -0.6 MPa, the treatment of seeds with 1.5 mM salicylic acid increased the percentage of germination, seed germination index, soluble carbohydrates and soluble proteins respectively 80.2, 169.4, 51.6, 93.4% and for the activity of ascorbate peroxidase, superoxide dismutase and catalase enzymes, 41.2%, 12% and 11.8%, respectively, compared to the control. Based on the research findings, pretreatment of salicylic acid (1.5 mM) is recommended in order to reduce the oxidative stress caused by drought for pumpkin.
Haniyeh Saadat; Mohammad Sedghi
Abstract
In order to investigate The effect of priming and aging on the growth indicators and activity of antioxidant enzymes in hybrid maize single cross 704 a factorial experiment was conducted based on a completely randomized design at the University of Mohaghegh Ardabili in 2021 with 3 replications. Treatments ...
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In order to investigate The effect of priming and aging on the growth indicators and activity of antioxidant enzymes in hybrid maize single cross 704 a factorial experiment was conducted based on a completely randomized design at the University of Mohaghegh Ardabili in 2021 with 3 replications. Treatments were aging (control, 7 and 14 days) and priming (control, hydro-priming, priming by gibberellin (20 mg / l) and salicylic acid (100 mg / l)). The results showed that aging decreased growth indicators including Radicle Length (RL), Plumule Length (PL), Seedling Length (SL), Radicle Dry Weight (RDW), Plumule Dry Weight (PDW), Radicle Fresh Weight (RFW), Plumule Fresh Weight (PFW) Seed Length Vigor Index (SLVI) and Seed Weight Vigor Index (SLWI), but types of priming, especially priming with gibberellin improved these traits. As compared to the control, the catalase, superoxide dismutase, and glutathione reductase activities increased respectively by 73%, 63%, and 21% after gibberellin treatment and 14 days of aging. The highest amount of peroxidase enzyme activity (1.797 units mg-1 protein) was related to gibberellin priming. In general, gibberellin pretreatment increased fragile corn seeds' growth indicators and antioxidant enzymes activities, and it can be used to enhance.aging compared to the control showed an increase respectively about 73%, 63% and 21%. The highest amount of peroxidase enzyme activity (1.797 units mg-1 protein) was related to gibberellin priming. In general, using gibberellin pretreatment strengthened weak corn seeds growth indicators and antioxidant enzymes and the treatment can be used to increase weak seed vigor.
Mahboubeh Hajiabbasi; Reza Tavakkol afshari; A.R. Abbasi; Reza Kamaei
Abstract
Glycine max is an important oilseed crop in all of the world. One of the problems of the G. max production is seed deterioration during seed storage, resulting in low germination and vigor before planting. In order to investigate the effects of ethylene and salicylic acid on the improvement of deteriorated ...
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Glycine max is an important oilseed crop in all of the world. One of the problems of the G. max production is seed deterioration during seed storage, resulting in low germination and vigor before planting. In order to investigate the effects of ethylene and salicylic acid on the improvement of deteriorated soybean seed, accelerated aging tests were conducted for 0, 6 and 10 days and natural aging test for 6 months. After the aging conditions, seeds were imbibed by 50 mM salicylic acid and 10 mM ACC (precursor of ethylene) for 6 hours at 25 °C. Also, a batch of the seed was used after the natural and accelerated aging tests without any hormonal treatment as a control (called dry seeds). Germination percentage, electrical conductivity and gene expression of AMY1, BMY1 were investigated in the dry seed, imbibition of water, salicylic acid and ACC at 6, 12 hours by the qRT-PCR method. The seed germination decreased and electrical conductivity increased with the progress of the aging. Gene expression varied in different days and hours. Salicylic acid and ACC had different effects on measured traits. The highest germination rate was observed in non-aged dry seeds (95%). On the other hand, ACC and salicylic acid failed to change seed germination of 10-day aged seeds. Moreover, the highest electrical conductivity (53 µScm-1gr-1) was in 10-day aged seeds affected by SA. The expression of AMY1 and BMY1 genes increased coincide with the age levels. Salicylic acid reduced the expression of AMY1 in non-aged seed, implicated that salicylic acid works as a stress factor in non-aged seeds. The expression of AMY1 gene in the non-aged seeds was 1.8, while it was 3.3 in 10-aged seed. Overall, aging caused disturbing of seed physiology and salicylic acid and ACC were not able to improve germination of deteriorated soybean seed
Tayyebeh Saadat; Mohammad Sedghi; Abdolghayyoum Gholipouri; Raouf Seyed Sharifi; Roghayyeh Sheikhbaglou
Abstract
To examine the effect of priming and deterioration on the activity of antioxidant enzymes, mobility of seed reserves in French bean, a factorial experiment was performed in a completely randomized design with three replications. Treatments included deterioration (control without deterioration, 88% and ...
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To examine the effect of priming and deterioration on the activity of antioxidant enzymes, mobility of seed reserves in French bean, a factorial experiment was performed in a completely randomized design with three replications. Treatments included deterioration (control without deterioration, 88% and 78% of germination of control) and priming (control, hydro-priming, priming by gibberellin and salicylic acid). The results showed that deterioration reduced germination percentage. Priming reduced the effect of deterioration and improved germination percentage. By increasing deterioration, seed reserves use efficiency (SRUE), seed reserves remobilization efficiency (SRRE) and fraction of used seed reserves (FUSR) were reduced, but respiration index (SR) and residual seed dry weight (RSDW) were increased. SRUE reduction was about 30% compared to the control treatment of deterioration and seed respiration index in gibberellin pre-treatment was higher about 24% compared to the control treatment without priming. The total seed protein content in gibberellin pretreatment and without deterioration was increased about 32 percent. The peroxidase activity in gibberellin treatment and deterioration 88% compared to the control showed an increase about 57%. The most superoxide dismutase activity (SOD, 175.2 unit mg-1 protein) was observed in priming with salicylic acid and deterioration 88%. The maximum amount of malondialdehyde (MDA, 1.46 mmol g-1 FW) was related to the treatment with no priming and deterioration 78%. In general, using gibberellin pretreatment strengthened weak bean seeds physiologically and the treatment can be used to increase weak seed vigor.
razieh sadat jahanmir; Reza Tavakkol afshari; Kazem Postini
Abstract
Dehydration or Aging is one of the vigor reducing and seed germination limiting agents. Inappropriate storage places, especially the gene banks, can speed up the aging process in a variety of seeds. In this study, to improve the adverse effects of seed aging, hormone treatments with GA, Cytokinin, Auxin ...
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Dehydration or Aging is one of the vigor reducing and seed germination limiting agents. Inappropriate storage places, especially the gene banks, can speed up the aging process in a variety of seeds. In this study, to improve the adverse effects of seed aging, hormone treatments with GA, Cytokinin, Auxin and Salicylic acid in concentrations of 50, 100 and 150 ppm (and distilled water as a control) on the quality of the naturally and synthetic deteriorated seeds of wheat were studied in the seed laboratory of Agriculture and natural resources college, Tehran University in 2015. After hormonal treatment, germination under salt stress in three levels, 0, -4 and -8 bar were investigated. The results showed that the naturally aged seeds had a higher mean value for all the traits. Treatment with cytokinin hormone showed higher percentage and germination rate and WGI between others hormones and all the hormonal treatments were better than control treatment (distilled water) in all traits and increased salinity stress tolerance. Salinity stress could affect all traits except the Mean Germinations Time.
Hassan Akhgari; Behzad Kaviani
Abstract
Priming has an important role on vigority of seed and plantlet. The first factor was two rice cultivars (Khazar and Hashemi) and the second one was ten priming treatment levels (T1-T10). Examination of seed vigority and seedling assessment was carried out according to the ISTA protocol. Results showed ...
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Priming has an important role on vigority of seed and plantlet. The first factor was two rice cultivars (Khazar and Hashemi) and the second one was ten priming treatment levels (T1-T10). Examination of seed vigority and seedling assessment was carried out according to the ISTA protocol. Results showed that the root fresh weight (14.7 mg) and root number (5) was higher in Khazar than those of Hashemi. The required time (hour) for reach to 50% germination (R50) was 50 hours for Khazar and 41 hours for Hashemi, accordingly Hashemi was more uniformity in germination than Khazar. Priming treatment with KCl created the highest electrical conductivity. The minimum time duration to reach to 5, 10 and 50% of germination with 13, 16 and 32 hours, respectively was related to hydro-priming treatment. Also, the highest germination rate was related to hydro-priming. Minimum required time duration until reach to 95% germination with 66 hours was calculated in hydro-priming of Hashemi. Most plantlet establishment on 20th day after cultivation in field was related to without priming-treated seed. Hydro-priming treatment is recommended to user due to low cost and positive effectiveness on acceleration and uniformity of germination in Hashemi.
M. Sharafizadeh
Abstract
In order to study effectpretreatment salicylic acid under drought stress on germination characteristics this research was conducted in May 1393. Barley seed varieties Zahak after soaking in solution [zero (water) 0.7 1.2 and 2.7 mM ) of salicylic acid (SA) for 24 hours under conditions of drought stress ...
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In order to study effectpretreatment salicylic acid under drought stress on germination characteristics this research was conducted in May 1393. Barley seed varieties Zahak after soaking in solution [zero (water) 0.7 1.2 and 2.7 mM ) of salicylic acid (SA) for 24 hours under conditions of drought stress on the germination dishes. Containing 10 ml of a solution of polyethylene glycol (6000) with potentials [zero (control), -0.5 MPa, 1 MPa and 1.5 MPa] moved, and related traits were determined germination. The results showed that the maximum effect of salicylic acid on germination time, reduce stress levels were low. The highest germination percentage (93 %) and the effective yield of seed soaking with the minimum concentration of salicylic acid 0. 7 mM ) and low levels of stress were gained. Vigor and Seedling vigor index at high concentrations of salicylic acid and high levels of stress decreased. In this study, drought stress reduced the germination traits and salicylic acid reduces the adverse effect of drought stress on germination was related traits.
Volume 4, Issue 1 , March 2015, , Pages 61-72
Abstract
The assessment of medicinal plants tolerance is very important for cultivation of them in saline areas. Salicylic acid is a growth regulator which its role was investigated in plants tolerance to environmental stresses such as salinity.In order to evaluate the effects of salicylic acid on early seed ...
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The assessment of medicinal plants tolerance is very important for cultivation of them in saline areas. Salicylic acid is a growth regulator which its role was investigated in plants tolerance to environmental stresses such as salinity.In order to evaluate the effects of salicylic acid on early seed germination andseedling growth of black cumin (Nigella sativa) under salt stress, an experimentwas conducted in a factorial arrangement based on a completely randomized design with 15 treatments and three replications. The experimental treatments were including different concentrations of salicylic acid (0, 0.25, 0.5, 0.75 and 1 mM) and salinity (0, 50 and 100 mM). The seeds of black cumin which were primed with different concentrations of salicylic acid for 24 hour, for germination test under salt stress, they transferred into sterile petri dishes which contain 10 ml NaCl. Results revealed a significant reduction in germination percentage and rate, seedling dry weight, seed vigor index, radicle length, radicle fresh and dry weight, shoot length, shoot fresh and dry weight with increasing salinity stress. Pretreatment with salicylic acid caused an increase of plant tolerance to salinity stress. The application of salicylic acid greatly caused the increment of germination percentage and rateand enhanced fresh and dry weight of black cumin under salt stress. It seems that higher concentrations of salicylic acid (0.5 up to 1 mM) caused an increase of all mentioned traits under normal and stress conditions.
Volume 1, Issue 2 , December 2012, , Pages 185-192
Abstract
In order to study on effect of Salicylic acid (SA) and Methyl Jasmonate on germination and seedling growth of canola Hyola401 cultivar, this research in laboratory and greenhouse were conducted based on completely randomized design (CRD) by four replications. Salicylic acid (SA) and Methyl Jasmonate ...
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In order to study on effect of Salicylic acid (SA) and Methyl Jasmonate on germination and seedling growth of canola Hyola401 cultivar, this research in laboratory and greenhouse were conducted based on completely randomized design (CRD) by four replications. Salicylic acid (SA) and Methyl Jasmonate concentration levels respectively were 0, 0.05, 0.1, 0.5, 1, 1.5 mM and 1, 3, 5, 10 and 12 andmicro;m and effects of those on germination percent, days number to 50% germination (G50), primary shoot and root length in laboratory and seedlings final emergence percent, days number to seedlings 50% emergence (E50), days number between seedlings 10 to 90% emergence (E10-90), seedlings chlorophyll content and remind seedlings number at end of experiment (seedling survival) were determined. Results showed that Salicylic acid (SA) and Methyl Jasmonate significantly improves germination percent, days number to 50% germination (G50), primary shoot and root lengthin canola seed compared with control. Also Salicylic acid (SA) and Methyl Jasmonate also increase chlorophyll content and seedling survival compared with control and among variousstudied Salicylic acid (SA) concentration levels 0.1 mM and 5 andmicro;m Methyl Jasmonate caused most improved germinatin characters.
