Mehdi Razmkhah; Ali Moradi; Hojatollah Latifmanesh; Hamidreza Balouchi
Abstract
Camelina (Camelina sativa) is a one-year oil plant, one of the most important problems of its cultivation is the interaction of seed germination and seed establishment with drought stress. Use of herbal treatments such as rye plant extract can speed up them, especially in drought-stress conditions. Therefore, ...
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Camelina (Camelina sativa) is a one-year oil plant, one of the most important problems of its cultivation is the interaction of seed germination and seed establishment with drought stress. Use of herbal treatments such as rye plant extract can speed up them, especially in drought-stress conditions. Therefore, this research was conducted to investigate the effect of priming and priming time on germination and some biochemical traits of Camelina under drought stress in the Agriculture and Genetics Laboratory of Yasouj University from 2021 to 2022. The experiment was conducted as a factorial in a completely randomized design with 4 replications. The first factor was priming with Danko variety of rye plant root extract at 3 levels (zero, 40 and 60%), The second factor was priming times at three levels (zero, 6 and 12 hours) and the third factor included drought stress at three levels (zero, -3 and -6 times). Based on the obtained results, it was observed that drought stress affected the biochemical indicators and germination of Camelina and pre-treatment with rye extract at a concentration of 60% along with prime in 12 hours with the improvement of some biochemical indicators and germination were able to increase the tolerance of Camelina seedlings under drought stress by affecting the non-enzymatic antioxidant defense system of the plant. The results of the experiment show that using Danko variety of rye root extract with a concentration of 60% in 12 hours improves physical characteristics of the seed, helping germination in optimal and drought stress conditions.
Seyed Keyvan Marashi; Zahra Albaji
Abstract
This research was conducted as a factorial in a completely randomized design with four replications. The studied treatments include salinity stress in the form of irrigation with normal water (0.4) and with salinity of 4 and 8 dS/m of NaCl salt and seed priming with hydrogen peroxide, including no priming ...
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This research was conducted as a factorial in a completely randomized design with four replications. The studied treatments include salinity stress in the form of irrigation with normal water (0.4) and with salinity of 4 and 8 dS/m of NaCl salt and seed priming with hydrogen peroxide, including no priming and seed priming in 25 and 50 mM solutions. In this experiment, the traits of germination percentage, germination rate, root length, stem length, root dry matter, stem dry matter and seed vigor index were investigated. In this research, the effect of salinity stress and hydrogen peroxide was significant on all investigated traits. The interaction effect of salinity stress and hydrogen peroxide on the studied traits was significant except germination rate. The maximum percentage of germination, length and dry matter of root and stem and seed vigor index was obtained under absence of stress (normal water) and application of 50 mM hydrogen peroxide and the minimum was under salinity of 8 dS/m and no application hydrogen peroxide. The maximum germination rate was obtained in the 50 mM hydrogen peroxide and no salt stress. In general, the results showed that the use of hydrogen peroxide in the presence of salinity stress was effective through the improvement of indices, and this can be considered as a strategy for better and proper seedling establishment.
Elham Bahrabadi; R. Tavakkol Afshari; Mehdi Nassiri Mahallati
Abstract
In order to evaluate the effect of temperature, drought and priming on the percentage and rate germination of corn seeds, a factorial experiment based on a completely randomized design with four replicates was conducted. Treatments included eight temperature (5, 10, 15, 20, 25, 30, 35, 40 and 45 ºC), ...
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In order to evaluate the effect of temperature, drought and priming on the percentage and rate germination of corn seeds, a factorial experiment based on a completely randomized design with four replicates was conducted. Treatments included eight temperature (5, 10, 15, 20, 25, 30, 35, 40 and 45 ºC), four drought potential (0, -0.4, -0.8 and -1.2 MPa), and five priming (control, hydroprimin, GA priming, ABA priming and, SA ptiming). Both percentage and rate of germination were inhibited at osmotic potentials ≤−0.8 MPa PEG. At 35 ºC temperature. Germination percentage of unprimed seeds was reduced from 94 to 36% in −1.2 MPa. Under these conditions, gibberellic acid hormone treatment improved seed germination. The effect of temperature on germination can be defined in terms of cardinal temperatures. We compared three non-linear regression models (Dent-like, segmented and beta). The outcome revealed that the Dent-like function was suitable for use in describing seed germination response to temperature. The base, optimum 1, optimum 2 and ceiling temperature were estimated to be 9.51, 24.12, 35 and 44.62 °C, respectively. The response of germination rate to both temperature and water potential can be described as a non-linear function of the hydrothermal model. According to the results of this study, hydropriming, gibberellic acid, and salicylic acid increased the mean deviation of the base temperature compared to non-priming treatment, increasing the standard deviation of the base temperature indicates the ability of seed germination in different osmotic potentials.
parisa sheikhnavaz jahed; mohammad sedghi; raouf seyedsharifi; omid sofalian
Abstract
In order to investigate the effect of priming on different aspects of germination of aged pumpkin seeds under drought stress, a factorial experiment was conducted based on a completely randomized design with four replications in the laboratory. Treatments included different levels of aging (control, ...
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In order to investigate the effect of priming on different aspects of germination of aged pumpkin seeds under drought stress, a factorial experiment was conducted based on a completely randomized design with four replications in the laboratory. Treatments included different levels of aging (control, 85% and 75% of control germination), salt stress (0, 75 and 150 Mmol) and priming (control, hydro, gibberellin, GR24, benzyl aminopurine and spermidine). The seeds reached the desired vigor level by accelerated ageing test. Morphological traits were also studied using seedling growth test. Mobility of seed lipid reserves, carbohydrate, number of photosynthetic pigments, photosynthesis, α-amylase and the amount of sodium ions were measured. In the study of germination traits, motility of lipid reserves, carbohydrates, chlorophyll a, photosynthesis and sodium content, salinity stress, seed deterioration and priming led to a significant change in them compared to the control seed. While in the case of shoot and root dry weight, chlorophyll b, and chlorophyll a / b ratio under salinity, seed deterioration and priming, no statistically significant change were compared to the control seed, the greatest effect of gibberellin-related seed priming was observed on germination traits and spermidine on physiological traits.
Masoumeh Asadi Aghbolaghi; Farzaneh Razavi
Abstract
This study was performed to investigate the effect of pumpkin (Cucurbita pepo L.) seed’s pre-treatment by salicylic acid and ascorbic acid on different aspects of seed germination and biochemical features. A factorial experiment was conducted based on a completely randomized design with three replications. ...
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This study was performed to investigate the effect of pumpkin (Cucurbita pepo L.) seed’s pre-treatment by salicylic acid and ascorbic acid on different aspects of seed germination and biochemical features. A factorial experiment was conducted based on a completely randomized design with three replications. Experimental treatments included two levels of seed maturity (normal seeds and aged seeds) and five levels of priming (control, ascorbic acid 50 and 100 mg/l, and salicylic acid 50 and 100 mg/l). The seed traits were finally measured included the germination percentage, first and second seed vigor index, seedling dry weight, peroxidase and superoxide dismutase activity, electrolyte leakage, soluble protein, and soluble carbohydrate. Based on the results, the seed pre-treatment with 100 mg/l ascorbic acid and salicylic acid could perform the highest germination indices. Moreover, the germination percentage (74%) was improved in aged seeds pre-treated by ascorbic acid and salicylic acid (especially at a concentration of 100 mg/l). Also, the antioxidant enzyme activity was increased while the cell electrolyte leakage was significantly decreased. In total, results indicate, the pre-treatment with salicylic acid and ascorbic acid, not only improved the germination quantity and vigor but also improved the germination quality mainly by the development of carbohydrate and protein content in pumpkin seeds.
Hossein Reza Rouhi; mohammad hasan vafaei; Maryam Saman; Alireza Shahbodaghlo
Abstract
Germination of seeds, one of the most critical stages of plant cycle is greatly influenced by drought stress. In this way, the effects of ascorbic acid to improve germination and antioxidant enzyme activities in sheep fescue seeds under drought stress were studied. A factorial experiment based on completely ...
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Germination of seeds, one of the most critical stages of plant cycle is greatly influenced by drought stress. In this way, the effects of ascorbic acid to improve germination and antioxidant enzyme activities in sheep fescue seeds under drought stress were studied. A factorial experiment based on completely randomized design with three replications was done. None- primed seed (control group) and priming of different concentrations of ascorbic acid (0, 1 and 2 mM) under 0, -0.4, -0.8 and -1.2 MPa drought stress levels were evaluated. The results showed that seed priming with ascorbic acid in different drought levels increased some traits such as germination percentage, vigour index, plumule and radicle length, soluble carbohydrates and protein contents and antioxidant enzymes activities (catalase, superoxide dismutase and ascorbate peroxidase). While it decreased mean germination time, electrical conductivity and malondialdehyde content in comparison to nonprime seeds. So seed priming with 1 and 2 mM of ascorbic acid in -1.2 MPa level increased vigour index, soluble carbohydrates and proteins, respectively by 167.03, 22.26, 35.77, 187.91, 22.61 and 30.20% compared to nonprimed seeds. Also, it increased activity of catalase, superoxide dismutase and ascorbate peroxidase, respectively by 11.85, 22.81, 12.25, 13.83, 31.33 and 9.93% compared to non-primed seeds. Therefore, the use of 1 and 2 mM ascorbic acid concentrations to reduce the negative effects caused by drought stress is recommended.
Abstract
In order to investigate the effect of seed bio-inoculation on some germination indices of Cumin under drought stress a two factors experiment was laid out. Experimental factors included priming in nine levels (bio- inoculation with 4 strains of PF1, PF2, PF75 and CHA0 of Pseudomonas fluorescent, and ...
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In order to investigate the effect of seed bio-inoculation on some germination indices of Cumin under drought stress a two factors experiment was laid out. Experimental factors included priming in nine levels (bio- inoculation with 4 strains of PF1, PF2, PF75 and CHA0 of Pseudomonas fluorescent, and 4 strains T29, T36, T39 and T40 of Trichoderma harzianum and non-primed (control)) and three levels of drought stress (0, -3 and -6 bar imposed by PEG 6000). Results showed that by increasing the osmotic potential levels germination indices decreased. At all levels of stress, inoculated seeds had higher germination percentage and root length than non-inoculated seeds. In under stress level of -3 bar, highest germination percentage (86.66%) and seedling weight vigor index (11.93) was obtained from T36 strain that; this treatment was able increase germination by more than 35% than non-inoculated seed. Under -6 bar, the highest (76.68%) and lowest (55%) germination percentage was related to T29 that has no significant difference with T36 (75.83%) and T39 (75.83%) and the lowest amount was related to non-primed treatment (55%). Also at all stress level, the highest length seedling vigor index was related to CHA0 strain. The highest weight seedling vigor index was related to T36 strain (6.4, 5.49 and 4.7 respectively in 0, -3 and -6 abr) and the lowest (4.89) was related to non-inoculated (control) seeds. According to the results, T36 can be suggested as a suitable pretreatment in moderation of the negative effects of osmotic potential on germination of cumin seeds.
Maryam Palizdar; Reza Tavakkol Afshari; Ziba Jamzad; Mohammadreza Ardakani; Parisa Nejat Khah
Abstract
This research was carried out to evaluate the germination response of eleven Salvia species to application of Gibberellic acid (GA3) and pre-chilling treatments in the Iran Research Institute of Forests and Rangelands during 2012. Germination responses of the seeds were investigated under control treatment, ...
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This research was carried out to evaluate the germination response of eleven Salvia species to application of Gibberellic acid (GA3) and pre-chilling treatments in the Iran Research Institute of Forests and Rangelands during 2012. Germination responses of the seeds were investigated under control treatment, 2-levels of Gibberellic acid (GA3) treatments with 500 mg/L (for 24 and 48 hours) and 2-levels of pre-chilling treatments at 4°C (for 2 and 4 weeks). The germination percentage and rate, as the physiological traits of the species, were studied based on a completely randomized design with four replications. Based on the results, applying the Gibberellic acid (GA3) treatment for 24 hours increased both the germination percentages and rates as compared to control; but the treatment for 48 hours was insignificantly changed the two mentioned parameters comparing to control. On the other hand, the pre-chilling treatment of seeds for 2 weeks also caused a significant increase in the above parameters as compared to the control treatment; while they were insignificantly different from the control treatment by the use of pre-chilling treatment for 4 weeks.
Tayyebeh Saadat; Mohammad Sedghi; Abdolghayyoum Gholipouri; Raouf Seyed Sharifi; Roghayyeh Sheikhbaglou
Abstract
To examine the effect of priming and deterioration on the activity of antioxidant enzymes, mobility of seed reserves in French bean, a factorial experiment was performed in a completely randomized design with three replications. Treatments included deterioration (control without deterioration, 88% and ...
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To examine the effect of priming and deterioration on the activity of antioxidant enzymes, mobility of seed reserves in French bean, a factorial experiment was performed in a completely randomized design with three replications. Treatments included deterioration (control without deterioration, 88% and 78% of germination of control) and priming (control, hydro-priming, priming by gibberellin and salicylic acid). The results showed that deterioration reduced germination percentage. Priming reduced the effect of deterioration and improved germination percentage. By increasing deterioration, seed reserves use efficiency (SRUE), seed reserves remobilization efficiency (SRRE) and fraction of used seed reserves (FUSR) were reduced, but respiration index (SR) and residual seed dry weight (RSDW) were increased. SRUE reduction was about 30% compared to the control treatment of deterioration and seed respiration index in gibberellin pre-treatment was higher about 24% compared to the control treatment without priming. The total seed protein content in gibberellin pretreatment and without deterioration was increased about 32 percent. The peroxidase activity in gibberellin treatment and deterioration 88% compared to the control showed an increase about 57%. The most superoxide dismutase activity (SOD, 175.2 unit mg-1 protein) was observed in priming with salicylic acid and deterioration 88%. The maximum amount of malondialdehyde (MDA, 1.46 mmol g-1 FW) was related to the treatment with no priming and deterioration 78%. In general, using gibberellin pretreatment strengthened weak bean seeds physiologically and the treatment can be used to increase weak seed vigor.
zahra rezaloo; samira shahbazi; hamed askari
Abstract
The present study was conducted to investigate the effect of biopriming on a number of selected species of Trichoderma fungi (T. harzianum, T. viride, T. konigii, T. virens and T. atroviridae( on vegetative and morphological traits of seedlings from sweet corn, wheat and sugar beet. Also, the effect ...
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The present study was conducted to investigate the effect of biopriming on a number of selected species of Trichoderma fungi (T. harzianum, T. viride, T. konigii, T. virens and T. atroviridae( on vegetative and morphological traits of seedlings from sweet corn, wheat and sugar beet. Also, the effect of gamma-mutation on Trichoderma genome and its effectiveness was investigated. The experiment was conducted with three levels of inoculation; control, inoculation with Trichoderma mutant and inoculation with Trichoderma wild type in a completely randomized design with three replications at the Nuclear Agriculture Research Institute, done. Germination percentage and length, weight and seedling traits were evaluated based on the ISTA method. The results showed that maize seed biopriming with formulation of Trichoderma species (wild type and mutant) had a superiority in dry weight and Vigor index than control, and in other traits it was also observed improvement. Germination of wheat treated Trichoderma was 100%. Seedling length increased, but other traits had no significant statistical difference. Treatment with Trichoderma fungi in sugar beet had no favorable effect on measured traits. Totally, the results showed that biopriming of seeds with Trichoderma fungi in wheat and maize improved plant growth parameters, especially in the stages of its establishment and its initial growth. but, this method is not generalizable for all plants and should be studied in detail.
Ameneh Akbarzadeh Sharafi; Hamid Reza Eisvand; N. Akbari; D. Goodarzi
Abstract
In order to study effects of seed priming with Gibberellin (GA), Ascorbic acid (AsA) and Salicylic acid (SA) on mitigation of seed deterioration damage and improvement of seed and seedling qualities of three sunflower cultivars (Farrokh, Barzegar and Shamshiri), a pot experiment was done in 2016. This ...
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In order to study effects of seed priming with Gibberellin (GA), Ascorbic acid (AsA) and Salicylic acid (SA) on mitigation of seed deterioration damage and improvement of seed and seedling qualities of three sunflower cultivars (Farrokh, Barzegar and Shamshiri), a pot experiment was done in 2016. This research was carried out as a factorial experiment based on RCBD with three replications. Seeds were exposed to accelerated aging then primed. Priming treatments were included: control, hydropriming, GA (50 and 75 ppm), AsA (100 and 150 ppm), SA (138 and 207 ppm), GA 50 ppm + AsA 100ppm, GA50 ppm + SA 138 ppm, AsA 100ppm + SA 138ppm, and GA 50ppm + AsA 100ppm + SA 138 ppm. Barzegar cultivar had better seedling emergence percentage and rate than the Shamshiri and Farrokh. Seedling emergence was improved by priming treatments; however, suitable treatments were different for the cultivars. Maximum seedling emergence percentage was obtained in Barzegar by GA 50ppm + SA 138ppm; however in Shamshiri and Farrokh by hydropriming. Priming improved seedling emergence rate of the three cultivars, but the response to priming was cultivar specific. Priming decreased seedling malondialdehyde (MDA) significantly in three cultivars. The lowest MDA content was obtained in Barzegar cultivar primed by AsA + SA.
M.A. Askari Sarcheshmeh; Z. Moradi Shakoorian; M. Delshad; R. Tavakkol Afshari
Abstract
In order to study the effect of priming on onion seed germination of Zangan Cultivar in different temperature conditions, this study was carried out with 6 levels of temperature of 5 to 30 ° C and three seed pretreatment, include control, hydro-priming and halo-priming with 0.5% potassium nitrate ...
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In order to study the effect of priming on onion seed germination of Zangan Cultivar in different temperature conditions, this study was carried out with 6 levels of temperature of 5 to 30 ° C and three seed pretreatment, include control, hydro-priming and halo-priming with 0.5% potassium nitrate with three replicate. The results showed that priming treatment with potassium nitrate (0.5%) could be effective on germination components. Primed seeds with potassium nitrate (0.5%) showed more germination at all temperatures. Potassium nitrate (0.5%) treatment also affected the germination reaction to temperature. These seeds showed 33.6% more germination than the other two treatments at 5°c. The results showed that germination percentage at 20°c in all treatments reaches its maximum and then remained relatively constant. In evaluating the segmented model for predicting cardinal temperatures, seed germination at base, optimum and ceiling temperatures was 1.7, 27.38, 46.09°c(in potassium nitrate (0.5%) treatment), 1, 25, 43°c (in hydro-priming treatment) and 1.9, 27.6, 46.12°c (in control treatment). For predicting time of germination at different constant temperatures used Thermal-time that constant coefficient of Thermal-time was 1872.79(°Ch).
Saeed Moori; Hamid Reza Eisvand; Ahmad Ismaili; Shahryar Sasani
Abstract
Germination and seedling production have an important role in crop production. This stage is influenced by quality of the seed strongly. Seed priming with gibberellic (GA3 100 ppm) acid and brassinosteroid (BR 100 ppm) on deteriorated wheat (Triticum aestivum. L) seeds were studied. A factorial experiment ...
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Germination and seedling production have an important role in crop production. This stage is influenced by quality of the seed strongly. Seed priming with gibberellic (GA3 100 ppm) acid and brassinosteroid (BR 100 ppm) on deteriorated wheat (Triticum aestivum. L) seeds were studied. A factorial experiment based on a completely randomized design with three replications was conducted in 2016. Treatments were seed aging (non-aged and accelerated aged) and seed pretreatment with GA3 and BR. No priming and hydro priming also were used as controls. Analysis of variance showed that the interaction of accelerated aging and priming was significant for germination percentage, plumule length, R/P L, germination rate, MDG, and GI; and for radicle length, DGS and CVG at 5% level. However, on the seedling length and mean germination time was not significant. The results showed that accelerated aging significantly reduced the soluble protein in seedlings and in turn caused a significant increase in malondialdehyde, soluble sugar, and proline content. The results showed that, the germination characteristics were improved under GA3 treatment.
R. Afrosheh; Hamidreza Balouchi; M. Movahedi Dehnavi; M.H. Gharineh
Abstract
The seeds of oilseed plants are susceptible to deterioration and the seed power in oilseed plants decreases faster over the time. In order to evaluate the effect of salicylic acid and seed aging on germination characteristics and biochemical changes of Carthamus tinctorius L. cv. Soffeh seeds,an experiment ...
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The seeds of oilseed plants are susceptible to deterioration and the seed power in oilseed plants decreases faster over the time. In order to evaluate the effect of salicylic acid and seed aging on germination characteristics and biochemical changes of Carthamus tinctorius L. cv. Soffeh seeds,an experiment was conducted as a factorial base on completely randomized design with fourand three replications for germination and biochemical characteristics respectively. The first factor was priming by salicylic acid include seven levels in 0 (distilled water), 50 and 100 ppm for 24 and 48 h in 15°Cand without priming (control)and secondary factor was combinations four levels of aging (0, 2, 4 and 6 days). Results showed that the interaction of priming and aging effects on germination rate, normal seedling percentage, abnormal seedling percentage, seedling length, length of seed vigor index, seedling dry weight, weight of seed vigor index and EC were significant, but on germination percentage, total protein, catalase and ascorbate peroxidase only affected by the main effects.Results showed that germination characteristics with increasing in the aging duration were reduced significantly and using priming germination characteristics were increased but protein, catalase and ascorbate peroxidase decline along with increasing seed aging. In the study, using priming treatment salicylic acid 50 ppm for 48 h had higher germination characteristics and antioxidant activity and soluble proteins in comparison with untreated or control of safflower seeds.
M. Nemati Khoei; A. Abbasi Surki; S. Fallah
Abstract
Optimizing the best methods to enhancement of seed germination and early seedling growth of Hypericum perforatum L. an experiment was conducted in Shahrekord University in two stages. In the first stage, a factorial experiment was done in a randomized complete block design with four replications in seed ...
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Optimizing the best methods to enhancement of seed germination and early seedling growth of Hypericum perforatum L. an experiment was conducted in Shahrekord University in two stages. In the first stage, a factorial experiment was done in a randomized complete block design with four replications in seed science and Technology lab, Faculty of Agriculture. Treatments consisted osmopriming with PEG in five levels (0, -3, -6, -9 and -12 bar) as first factor and application of GA in four levels (0, 500, 1000 and 1500 ppm) as second factor. results showed that PEG -12 bar + GA 1000 ppm with an average of 91.5% ranked the highest percentage of germination which had no significant difference with PEG -9 bar + GA 1500 ppm and PEG -9 bar + GA 1000 ppm respectively with the germination percentage average 88 and 88.5%, while the control seed with an average (53.5%) had the lowest percentage of germination. As well as combination treatments PEG -9 bar + GA 1000 ppm increased seedling length, seedling dry weight and seed vigor and increased them respectively as by as 1.19, 1.3, 1.96 and 2.14 times than control. The second experiment was conducted in a randomized complete block design with three replications in the research greenhouses conditions, and treatments consisted of the best treatments of first experiment and control. In greenhouse conditions osmopriming PEG -12 bar and application of GA 1000 ppm with an average of 82% ranked the highest percentage of emergence which had better performance than the control with an average of 41.33 %. Finally to enhance seed performance of medicinal plant Hypericum perforatum L., it could be recommended osmopriming with PEG -12 bar and application of GA 1000 ppm or PEG -9 bar + GA 1000 ppm.
S.R. Khatami; M. Sedghi; R. Seyed Sharifi
Abstract
In this research effect of priming types was investigated on the activity of antioxidant enzymes including Catalase (CAT), Ascorbate peroxidase (APX), Superoxide dismutase (SOD), Malone dialdehyde (MDA) and Gluthation reductase (Gred) in hybrid maize single cross 704 under drought stress. Treatments ...
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In this research effect of priming types was investigated on the activity of antioxidant enzymes including Catalase (CAT), Ascorbate peroxidase (APX), Superoxide dismutase (SOD), Malone dialdehyde (MDA) and Gluthation reductase (Gred) in hybrid maize single cross 704 under drought stress. Treatments were drought at 4 levels (0, -3, -6 and -9 bar) and priming (Hormone, hydro, osmo, ascorbic and control). Germination percentage, seedling vigor and the activity of antioxidant enzymes were measured. Results showed that interaction of drought and priming was significant an all traits except of germination percentage. The highest seedling vigor (15.996) obtained from control condition. The highest CAT activity (35 μmol min-1 mg-1) observed in ascorbic acid priming at -9 bar drought stress. Also, the highest APX activity was related to hormone priming and -9 bar. While the lowest activity observed in control drought and osmo-priming. The highest amount of MDA achieved in -9 bar and control priming. The highest SOD activity observed in -9 bar. The highest Gred activity obtained by ascorbic acid priming at -9 bar.
V. Mansouri Gandomani; H. Omidi
Abstract
The experiment was conducted to examine the effects of nano-particle silicon dioxide to improve germination of deteriorated soybean seeds (Williams). The experiment was done as factorial in a completely randomized design with three replications in 2015 in Seed Technology Laboratory in the University ...
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The experiment was conducted to examine the effects of nano-particle silicon dioxide to improve germination of deteriorated soybean seeds (Williams). The experiment was done as factorial in a completely randomized design with three replications in 2015 in Seed Technology Laboratory in the University of Shahed. The first factor involves different levels of accelerated aging including control and accelerated aging for 24 and 48 hours at 41 ° C in high humidity conditions and different concentrations of nano-silica as the second factor including zero (control), 40 and 60 ppm, respectively. The traits such as germination percentage, germination rate, the average duration of germination, the number of normal seedlings, seedling fresh weight, root to shoot ratio, relative water content of root, seedling length and chlorophyll content were measured. The results showed that the effect of nano-silica pre-treated soybean seed germination was significant on all traits. But the interaction of nano-silica priming and the deteriorated seeds of soybean was significant only in some germination characteristics. Pre-treatment of 60 ppm nano-particle silica increased 20% of deteriorated seeds germination rate and reduced the average time of germination. Pre-treatment of 40 ppm increased about 7-10% of the number of normal seedlings, and also treatment of silica nanoparticles improved seed germination with compensate amount of chlorophyll in deteriorated seeds.
M.K. Souri; M.A. Arab; GH. Tohidloo; A.K. Kashi
Volume 5, Issue 2 , January 2016, , Pages 85-94
Abstract
Artichoke as a vegetable crop has difficulties regarding seed germination and uniformity of germinated seedlings. This experiment was done to evaluate the effects of some seed priming treatments on germination quality in Artichoke. The experiment carried out as a factorial based on a completely randomized ...
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Artichoke as a vegetable crop has difficulties regarding seed germination and uniformity of germinated seedlings. This experiment was done to evaluate the effects of some seed priming treatments on germination quality in Artichoke. The experiment carried out as a factorial based on a completely randomized design under laboratory conditions. Treatments were control (without any treatment), and seed priming with distilled water, potassium nitrate, calcium nitrate, calcium sulfate in the same concentrations of 10 mM, as well as PEG6000 with osmotic potential of 0.5 MP, and two priming time of 12 and 24 hour. The results showed that the highest root length was detected in KNO3 and d-water treatments, and the lowest root length control treatment. The highest stem length was in KNO3 that showed no difference with CaNO3. The highest seedling length was in 12 h KNO3 treatment which had significant difference to other treatments. The highest seedling fresh weight was in 12 h KNO3, 24 h CaNO3 and 24 KNO3 without significant difference among them. Regarding germination percentage, 12h and 24 h KNO3 treatments led to the highest germination percentage, while 24 h CaNO3 showed the highest germination rate. The highest and the lowest abnormal seedlings were in 24 h PEG and CaNO3 treatments, respectively. Therefore, priming for 12 h using potassium nitrate or 24 h using calcium nitrate for improving germination characteristics of artichoke is suggested.
F. Nabi; A. Asgharzadeh; E. Ganji; A.R. Bagheri
Volume 5, Issue 1 , March 2015, , Pages 123-134
Abstract
Regarding importance of homogenous germination and to accelerate this stage of Aquilegia chrysantha a study was conducted with purpose of identification of different morph of seeds of A. chrysantha. Therefore, first the black and brown seeds were separated visually and then divided in two groups in term ...
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Regarding importance of homogenous germination and to accelerate this stage of Aquilegia chrysantha a study was conducted with purpose of identification of different morph of seeds of A. chrysantha. Therefore, first the black and brown seeds were separated visually and then divided in two groups in term of size using machine vision method. The seeds were stored for four weeks at 4ₒC inrder to stimulate germination and after that the treatments of 4, 8, 12, 24 hours of hydropriming and control were applied on the seeds. The study was factorial and conducted in a completely randomized design. The factors include color and size of seeds and also hydropriming treatments. The results showed that large seeds in comparison with small seeds were better in traits including seed germination percentage, germination rate, and mean germination time. In addition black seeds have a better performance in mentioned traits than brown seeds. Also the best characteristics of germination were recorded in the 12 and 24 hours of hydropriming. Overall, using large black seeds in the 12 hours of hydropriming would have a good germination and is recommended for better production of A. chrysantha.
Volume 3, Issue 2 , December 2014, , Pages 137-151
Abstract
During seed storage, some seeds traits might change and seed vigor may decrease in bad storage situation, to determine the effect of some hormones in different concentrations, the study was conducted in biotechnology laboratory of Agriculture University of Tehran in 2012. In this study, seeds aging were ...
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During seed storage, some seeds traits might change and seed vigor may decrease in bad storage situation, to determine the effect of some hormones in different concentrations, the study was conducted in biotechnology laboratory of Agriculture University of Tehran in 2012. In this study, seeds aging were tested for 0, 2 and 4 days and after they were primed with distilled water, salicylic acid, IAA and kinetin. Traits measured included germination percent, germination rate, shoot length, root length, seedling dry matter and vigor. The results showed that hydro priming improved many characteristics in fresh seeds but it's not very useful for seeds under ageing conditions. For rising seed vigor in these cases, it's better to use hormones, especially salicylic acid which is called tolerant hormone. Using IAA because of broadleaf control property, it doesn’t seem logically. The best treatment in this study was salicylic acid in low concentration
Volume 2, Issue 1 , March 2013, , Pages 25-38
Abstract
This study did with the aim of investigating osmopriming effect on phonological characteristics and some germination features in hybrid Maize (Zea mays L. CV. Single cross 704 and 640). This was carried out with a factorial experiment in randomized completely block design with tree on four replications, ...
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This study did with the aim of investigating osmopriming effect on phonological characteristics and some germination features in hybrid Maize (Zea mays L. CV. Single cross 704 and 640). This was carried out with a factorial experiment in randomized completely block design with tree on four replications, in seeds field and lab research which performed in Mazandaran naturon resources and cultivation, modification plants group, 2010-2011 mordad, respectively. Treatments included seed of two kind maize (640 and 704) and seven polyethilen glycol solution with 5 and 10 percent densities, potassium nitrat with 0.5 and 1 percent densities and choloride potassium with 1 and 2 densities, water and control (with out priming). Seedling emergence percent, Seedling emergence rate , Established seedlings, Day number sowing to rise coleoptiles, Day number sowing to stage 4 and 8 leaf, Day number to emergence tasel, Day number to the end of milk stage achieved, Germination percent, Germination rate, Normal seedlings number, Mean daily germination,Germination rate indx, Mean time to germination and Vigor 2 index.However, none of characteristics from statistican point under numbers in fluence showed meaningful difference, but results indicated that more percent and maize dermination rate achieved respectively with priming by polyethilen glycol solution with 5 and 10 percent densities. Which, most established seedlings to rise coleoptile, days number sowing to stage 4 and 8 leaf, days number to emergence tasel resulted with priming by control (with out priming) and their lowest was for polyethilen glycol with 5 and 10 percent respectively. Also, most of days number to the end of milk stage achieved with priming by potassium nitrate and choloride with 0.5 1 and 2 percent respectively. Most percend and germination rate for hydropriming and control (with out priming) and most normal seedling number and mean daily germination for water and control were obtained. Maximum of germination rate index and mean daily germination for control prime (with out priming) and minimum of mean germination time with priming by potassium nitrat and choloride with 0.5 and 4 percent obtained and also maximum of vigour 2 index for hydropriming and it ,s minimum was achieved for potassium nitrate and cholorid priming solution with0.5 and 4 percent resulted. Hence, both field and lab situations statistically were not under interaction effect on variety andtimes; priming and they havenandrsquo;t meaningful difference.
Volume 2, Issue 1 , March 2013, , Pages 55-64
Abstract
In order to study effect of seed priming on seedling emergence and vigour of wheat cultivar ,experiment was conducted in laboratory seed and field ,in seed andamp; plant certification and registration Institute in 2009. Experimental design was a factorial based on completely randomized designs with four ...
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In order to study effect of seed priming on seedling emergence and vigour of wheat cultivar ,experiment was conducted in laboratory seed and field ,in seed andamp; plant certification and registration Institute in 2009. Experimental design was a factorial based on completely randomized designs with four replication. Two cultivars (Azar-2,Sardare-101)andtimes; four priming media and control (un soaked) were used in this experiment. Seeds were primed for 12 hour and 20 ċ in four priming media , PEG 10%, KCl 2%, KH2PO4 0/5%, distilled water and control. In pot experiment seedling emergence percent, seedling leugth, primary root and shoot leuth, and sedling dry weight, primary root and shoot dry weight and mean seedling emergence rate and time and in tield experimcut seedling emergence percent and mean rate and time detemined. Result vevealed . The interaction among seed priming media andtimes;cultivar showed the significant effects on stem length , root dry weight ,stem dry weight and total dry weight (andalpha;= %1), seedling length and Mean Emergence Rate (andalpha;=5%). All of the priming media caused that seeds emergence rapidly in compare with control. In compare among seed priming, Maximum root dry weight, stem dry weight and total dry weight was obtained for seeds osmoprimed with KCl 2%.The most percent emergence, mean emergence rate and seedling length was obtained for seeds osmoprimed with PEG 10%.In field condition hydro priming improved the rate of germination . In compare among seed priming, the most percent emergence in field was obtained in PEG 10%.The interaction among seed priming , seed priming media andtimes;cultivar showed the significant effects on percent emergence. The most was observed in sardari-101andtimes; KCl 2% and PEG 10%andtimes;Azar-2.
