Reza Rezvani; Behnam Kamkar; Zeinab Jabbari Badkhor
Abstract
To mitigate the detrimental effects of salinity stress on vegetable crops, various approaches, including the applying of organic inputs during different growth stages, such as germination, are employed. This study, conducted in 2023, aimed to assess the influence of humic acid pretreatment on the germination ...
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To mitigate the detrimental effects of salinity stress on vegetable crops, various approaches, including the applying of organic inputs during different growth stages, such as germination, are employed. This study, conducted in 2023, aimed to assess the influence of humic acid pretreatment on the germination characteristics and growth parameters of cucumber (Cucumis sativus) under salt stress. Two experiments, one in the laboratory and the other in a greenhouse, were carried out using a factorial design based on a completely randomized design with three replications. The experimental factors included humic acid priming at four concentrations (0, 50, 100, and 200 mg/l-1) and salinity at five levels (0, 25, 50, 100, and 150 mM NaCl). Results indicated that humic acid exerted a positive impact on various germination and growth attributes of cucumber, including germination percentage, germination rate, seedling length, dry weight, vigor index, and chlorophyll a, b, and carotenoid under salt-stressed conditions compared to the control. The treatment with 200 mmol/l humic acid and no salt exhibited the highest germination rate (87%) and seedling length (12.66 cm). The interaction between salinity and humic acid revealed that humic acid concentrations exceeding 100 mg/l-1 could alleviate the adverse effects of salinity stress on total chlorophyll. In conclusion, the cost-effective and easily applicable method of priming cucumber seeds with humic acid presents a potential strategy to mitigate the impacts of salinity stress in regions with saline water or soil.
Saeed Sadeghzadeh Hemayati; Reza Shariari; Ali Saremirad
Abstract
Suitable germination and crops establishment are one of the most significant and fundamental issues that overshadow the economic performance of the product. Improving the quality of seeds by relying on their pre-treatment is one of the issues that can be considered. Accordingly, the present study was ...
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Suitable germination and crops establishment are one of the most significant and fundamental issues that overshadow the economic performance of the product. Improving the quality of seeds by relying on their pre-treatment is one of the issues that can be considered. Accordingly, the present study was performed to investigate the effect of pretreatment of sugar beet seeds using humic in factorial experiment in 2017. The first factor was humic acid including four levels of zero, 8.33, 12.50, and 25.00 mg per 100 g of seed, and the second factor, cultivar included two levels of Ekbatan and Paya. According to the results of the analysis of variance, the main effects of humic acid and cultivar had a significant effect on all evaluated characteristics except root dry weight at one and five percent probability levels. Humic acid-cultivar interaction was significant only for the three characteristics of mean germination time, root dry weight, and shoot dry weight. Based on the mean comparison results, the seed vigor (87.87%), germination rate (6.27 bud/day), seedling vigor length index (3.52) and seedling vigor weight index (1269.96) were assigned to seed pretreatment using 12.50 mg of humic acid per 100 grams of seeds. Seed pretreatment without significant difference in the amount of coating material increased the uniformity of seed germination. Among the two studied cultivars, the Ekbatan cultivar was superior to the Paya cultivar in terms of all studied characteristics. In general, the use of humic acid improved the germination characteristics and early growth of seedlings.
Ahmad Afkari
Abstract
In order to investigate the effects of priming with salicylic acid and salinity stress on improvement of germination characteristics, activity of antioxidant enzymes and and membrane lipid peroxidation of wheat cultivar Mihan, an experiment was conducted as factorial in a completely randomized design ...
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In order to investigate the effects of priming with salicylic acid and salinity stress on improvement of germination characteristics, activity of antioxidant enzymes and and membrane lipid peroxidation of wheat cultivar Mihan, an experiment was conducted as factorial in a completely randomized design with three replications in the physiology lab at the Islamic Azad University of Kaleiber Branch in 2018.The treatments consisted of different concentrations of acid salicylic in four levels (0, 0.5, 1 and 1.5 mM) and salt stress was inducted by NaCl solution (0, 50 100, 150 mM). The results analysis of variance showed that the effects of salinity and salicylic acid on germination indices and activity of antioxidant enzymes was significant.Results comparison of data showed that NaCl priming by salicylic acid increased the germination percentage, root growth, root, seedling dry weight and decreasing activity of antioxidant enzymes and seed protein content in the salty environment. Salicylic acid increased the level of cell division of seedlings and roots which caused an increase in plant growth. Enzymes activity assay showed that enzyme activity was increased under salt stress conditions and SA reduced activity of antioxidant enzyme by decreasing the salinity effects. In brief, the SA treatment reduced the damaging action of salinity on seedling growth and accelerated a restoration of growth processes. It seems that Salicylic acid as a plant growth regulator under salinity condition, activated plant tolerance mechanisms to salinity condition and decrease damaging effect of salinity on seed germination and seedling growth of wheat.
Jalil Abaspour; Behrooz Salehi-Eskandari; Maryam Montazeri-Najafabadi
Abstract
Nickel is an essential microelement, but the increased concentration it in the medium disrupts the germination and growth of plants. In the present study, the effect of pretreatment and exogenous proline in the seedling growth of Cucumis melo under nickel stress was investigated. For this purpose, seeds ...
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Nickel is an essential microelement, but the increased concentration it in the medium disrupts the germination and growth of plants. In the present study, the effect of pretreatment and exogenous proline in the seedling growth of Cucumis melo under nickel stress was investigated. For this purpose, seeds were divided into two groups. In one group, seeds were sterilized for 24h, then they soaked in 0, 10 mM proline and finally cultured in different nickel concentrations. Another group seeds were soaked in deionized water for 24 hours and then cultured in petri dishes containing the same from one another concentrations of nickel and proline. All seeds were kept for 10 days, then measured germination, and growth parameters and membrane stability. The results showed that germination parameters were not affected by nickel. Radicle was more sensitive to Ni excess than hypocotyl. The amount of malondialdehyde increased with increasing nickel concentration. Add exogenous proline significantly reduces germination percentage and germination index but it increased mean germination time. Proline pretreatment in the presence of nickel treatments improved radical length, root to shoot ratio. But the addition of exogenous proline increased hypocotyl length while radical length and their ratio decreased in lower exposure levels. However, exogenous proline reduced lipid peroxidation and membrane permeability more than similar treatments under nickel treatments. Proline, as a pretreatment as a compatible solution and antioxidant, improves the early stages of growth and reduces the damage caused by nickel toxicity in the early stages of cucumber seedling growth.
B. Shadkam; Mohammed Hussain gharineh; Amin Lotfi Jalal Abadi; Seyed Amir Moosavi
Abstract
Salinity is one of the main limiting factors for seed germination and seedling growth. The use of nutrients in priming treatment is known as an effective way to improve seed yield In this regard, a factorial experiment was conducted in the Khuzestan University of Agricultural Sciences and Natural Resources, ...
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Salinity is one of the main limiting factors for seed germination and seedling growth. The use of nutrients in priming treatment is known as an effective way to improve seed yield In this regard, a factorial experiment was conducted in the Khuzestan University of Agricultural Sciences and Natural Resources, based on randomize complete design with three replications. Priming treatment with nano and micro magnesium was performed at five concentrations zero, 50, 100, 200, 400 mg /litr, sepcratly. The durations of 12 and 24 hour were used for seed priming and salinity stress levels were zero, 50, 100, 150, 200, 250 mM made with sodium chloride. The results showed that increasing salinity stress declined hyssop seeds resistance and reduced the germination characteristics of this plant. Priming with 400 mg/litr magnesium microclate produced the highest germination percentage of 89.3%, average germination time of 5.9, seed vigor of 27.2 and root length of 24 mm. The highest germination rate (0.461 per day) and shoot length (18 mm) were observed in control and 400 mg/litr of nano-chelate, respectively. Results showed that nutrient seed priming with micochelate effectively alleviated salinity stress effects and improved seed germination properties compared to nanochelate magnesium. The optimum seed priming treatment obtained from 400 mg/litr micro chelate magnesium for 12 hours.
