Hassan Nasiri Avanaki; Seyed MohammadReza Ehteshami; Mahmoud Bagheri
Abstract
In order to investigate the effect of planting date on germination characteristics and biochemical activity of seed in different quinoa genotypes, an experiment was conducted in the Research Laboratory of Seed Physiology and Biotechnology of the Faculty of Agricultural Sciences at University of Guilan ...
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In order to investigate the effect of planting date on germination characteristics and biochemical activity of seed in different quinoa genotypes, an experiment was conducted in the Research Laboratory of Seed Physiology and Biotechnology of the Faculty of Agricultural Sciences at University of Guilan in 2019. The experiment was performed as a split plot in the form of a Randomized Complete Block Design in three replications. The seeds of three genotypes T, R and Q29 were planted on six planting dates: 15 January, 15 February, 15 March, 15 February, 15 May and 15 June in the research farm of Karaj Seedling and Seed Breeding Research Institute. The harvested seeds from the treatments available in the field were transferred to the laboratory in order to investigate seed germination characteristics and vigour, and were measured germination percentage and rate, length and weight of the seedling, length and weight index of the seed vigour, electrical conductivity of the seed solution, and biochemical characteristics of soluble protein content, malondialdehyde content, alpha amylase, catalase and peroxidase enzyme activity. The interaction of two factors was significant on germination percentage, germination rate, number of abnormal seedlings, activity of alpha-amylase, catalase, peroxidase and superoxide dismutase enzymes, soluble protein content, malondialdehyde content and electrical conductivity of seed solution. The three planting dates of 15 January, 15 February, 15 March in Q29 genotype showed the highest percentage, germination rate, alpha-amylase enzyme activity and the highest number of abnormal seedlings.
mousavi seyyed mehdi; Heshmat Omidi; seyyed esmaeil mousavi
Abstract
To study of the effect of biological pre-treatments on germination and growth of Fenugreek (Trigonella foenum-graecum L.) seedling under natural salinity stress, a factorial experiment in a completely randomized design was conducted in three replications. Experimental treatments were biological pre-treated ...
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To study of the effect of biological pre-treatments on germination and growth of Fenugreek (Trigonella foenum-graecum L.) seedling under natural salinity stress, a factorial experiment in a completely randomized design was conducted in three replications. Experimental treatments were biological pre-treated at four levels (control, inoculation with Azoto bio-fertilizer, inoculation with Phosphate bio-fertilizer, inoculation with both bio-fertilizers) and natural salinity (salt of Qom Lake) at five levels (zero, 3, 6, 9 and 12 dS/m).The results showed that increasing of salinity levels led to increasing of mean germination time and using of bio-fertilizer treatments and combination treatment caused this index show 22 percentages reduction at highest level of salinity compared to control treatment at same level of salinity. Salinity reduced the germination coefficient and the highest amount of this index (74.3) obtained at 3 dS/m level of salinity that compared to control treatment and at the same level of salinity showed 89 percentages increasing. Salinity reduced total chlorophyll and using of biological treatments caused amount of total chlorophyll at zero level of salinity show 155 percentages increasing compared to control treatment and at the same level of salinity. With increasing of salinity level increased soluble protein and combination treatment increased this index 12 percentages at the highest level of salinity compared to control. Under stress, plants face with oxidative stress and to confront of that use antioxidant enzymes. In this experiment, the using of biological treatments by raising of the production of antioxidant enzymes caused the reduction of salinity effects.
