Forough Firouzkoohi; Fatemeh Nasibi; Batool Keramat; Hadi Noori; Effat Ahmadi Mousavi
Abstract
Effects of dielectric barrier discharge (DBD) Helium plasma treatments on guar (Cyamopsis tetragonoloba) seed germination and seedling growth were studied. Seeds were pretreated with 0, 1/44 and 0/68 W of cold plasma for 60 s. Salt stress experiment included three salinity levels of NaCl (0, 50 and 100 ...
Read More
Effects of dielectric barrier discharge (DBD) Helium plasma treatments on guar (Cyamopsis tetragonoloba) seed germination and seedling growth were studied. Seeds were pretreated with 0, 1/44 and 0/68 W of cold plasma for 60 s. Salt stress experiment included three salinity levels of NaCl (0, 50 and 100 mM). Results showed that plasma treatments had positive effects on seed germination and seedling (shoot length, root length, dry and fresh weight of shoot and root) , The treatment of 1.44 W of cold plasma had the most stimulating effect on the percentage of germination and growth of guar seedlings. Additionally, the application of Helium plasma treatments, considerably enhanced chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, and potassium(k) content in the leaves of guar plant. Thus, cold plasma treatment can be used an ameliorative way to the enhancement germination and improve seedling growth of guar against damage caused by salt stress. Key words: dielectric barrier discharge, seed germination, guar, cold plasma, salt stress
Sara Yavari Ramsheh; Fatemeh Dehghan Nayeri
Abstract
In this research, morphological and biochemical traits of 10 isolates of Pseudomonas fluorescens bacteria, salt tolerance and the effect of bacteria on germination and seedling growth indicators of sesame cultivars under salt stress were conducted. All isolates were Gram-negative and had positive motility ...
Read More
In this research, morphological and biochemical traits of 10 isolates of Pseudomonas fluorescens bacteria, salt tolerance and the effect of bacteria on germination and seedling growth indicators of sesame cultivars under salt stress were conducted. All isolates were Gram-negative and had positive motility and fluorescent properties. Among the bacteria, P2, P3 and P9 isolates were more capable of solubilizing inorganic phosphorus. The ability to produce siderophore was higher in some isolates and the isolates P1, P2, P3, P8, and P10 showed the highest in vitro salt-tolerance. A factorial experiment including two sesame cultivars, 4 salinity levels and inoculation with 3 isolates was done in the form of a completely randomized design in three replications. Based on the results, the effect of bacteria, salinity, variety and their interaction on the percentage and rate of germination, germination indices, allometric coefficient and seedling length and weight index were significant. Under salt stress, the germination and growth of seedlings significantly decreased but, the pretreatment of sesame seeds with salt-tolerant isolates increased the characteristics related to germination and growth indices of sesame cultivars. The highest effect on all parameters including germination rate belonged to P9. Therefore, P9 isolate can be used to increase the tolerance of sesame to salinity stress.
Behzad Gholipor; Afshin Mozaffari; Abas Maleki; Mohammad Mirzaei Heydari; Farzad Babaii
Abstract
Experiments were carried out in a completely randomized design with 3 replication factorial arrangement. Experimental treatments were including genotype factor (including 15 sea beet samples from Khuzestan, Elam, Kermanshah, Kurdistan and Azerbaiyjan provinces and two sensitive and susceptible cultivar ...
Read More
Experiments were carried out in a completely randomized design with 3 replication factorial arrangement. Experimental treatments were including genotype factor (including 15 sea beet samples from Khuzestan, Elam, Kermanshah, Kurdistan and Azerbaiyjan provinces and two sensitive and susceptible cultivar of sugar beet) and salinity factors (concentration of 0, 50, 100 and 400 mM) and drought factors (5 levels of 0, 3, 6, 9 and 12 bar). Results showed that the effect of salinity and drought treatments on germination percentage, radicle, plumule length, radicle, and plumule weight traits were significant and in both experiments with increasing stress intensity germination percentage, radicle and plumule length, radicle and plumule weight traits decreased. In both experiments, the germination percentage trait was less and hypocotyl weight trait more affected by stresses than other traits. Alsoو in both experiments, radicle growth was less affected by stress than the plumule. Statistical estimations showed that there was a significant difference between investigated genotypes for germination percentage, radicle length, plumule length, radicle weight and plumule weight in both experiments. In salinity and drought stress conditions, at least 5 and 7 sea beet genotypes were identified from Elam, Kermanshah, Khuzestan and Azerbaiyjan provinces, which were better in terms of germination and early growth characteristics than sugar beet cultivars. Based on this, it can be concluded that sea beet has high diversity and resistance potential against salinity and drought stresses during germination and early growth stages, and can be used to produce more resistance sugar beet against salinity and drought stresses.
