Reza Rezvani; Behnam Kamkar; Zeinab Jabbari Badkhor
Abstract
To mitigate the detrimental effects of salinity stress on vegetable crops, various approaches, including the applying of organic inputs during different growth stages, such as germination, are employed. This study, conducted in 2023, aimed to assess the influence of humic acid pretreatment on the germination ...
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To mitigate the detrimental effects of salinity stress on vegetable crops, various approaches, including the applying of organic inputs during different growth stages, such as germination, are employed. This study, conducted in 2023, aimed to assess the influence of humic acid pretreatment on the germination characteristics and growth parameters of cucumber (Cucumis sativus) under salt stress. Two experiments, one in the laboratory and the other in a greenhouse, were carried out using a factorial design based on a completely randomized design with three replications. The experimental factors included humic acid priming at four concentrations (0, 50, 100, and 200 mg/l-1) and salinity at five levels (0, 25, 50, 100, and 150 mM NaCl). Results indicated that humic acid exerted a positive impact on various germination and growth attributes of cucumber, including germination percentage, germination rate, seedling length, dry weight, vigor index, and chlorophyll a, b, and carotenoid under salt-stressed conditions compared to the control. The treatment with 200 mmol/l humic acid and no salt exhibited the highest germination rate (87%) and seedling length (12.66 cm). The interaction between salinity and humic acid revealed that humic acid concentrations exceeding 100 mg/l-1 could alleviate the adverse effects of salinity stress on total chlorophyll. In conclusion, the cost-effective and easily applicable method of priming cucumber seeds with humic acid presents a potential strategy to mitigate the impacts of salinity stress in regions with saline water or soil.
Azam Khaleghi; Alireza Dadkhah; Reza Rezvani
Abstract
This research was conducted in order to investigate the effect of aqueous extract of different organs of Artemisia aucheri on the growth and germination traits of Brassica napus and Goldbachia laevigata in two separate experiments including the laboratory and the greenhouse conditions, respectively. ...
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This research was conducted in order to investigate the effect of aqueous extract of different organs of Artemisia aucheri on the growth and germination traits of Brassica napus and Goldbachia laevigata in two separate experiments including the laboratory and the greenhouse conditions, respectively. The experiments were exerted based on a completely randomized design with three replications and randomized complete block design with four replications, respectively. The test treatments included aqueous extracts of Artemisia in concentrations of 5, 10 and 15% of roots, 5, 10 and 15% of aerial organs and control (distilled water). The results showed that with increasing extract concentration, the germination and growth traits and total chlorophyll of both investigated plants significantly decreased. In such a way that the highest and lowest inhibition rates were associated with 15% root extract and 5% aerial organs extract, respectively. The level of 15% Artemisia root extract in Brassica decreased the germination percentage by 37.1% and in Goldbachia by 48.5% compared to the control. Increasing of extract concentration from zero to 15% of arial parts decreased stem length decreased by 30.3% in Brassica and 56.3% in Goldbachia compared to the control. Most of the examined traits in Goldbachia weed, were more sensitive to the allelopathic effects of Artemisia extract compared to Brassica. in general, root extract of Artemisia were more capable of dealing with germination and seedling growth than the aerial organs. Therefore, 15% aqueous extract of Artemisia root can be suggested to reduce the growth and weakening of the weed Goldbachia.
mousavi seyyed mehdi; Heshmat Omidi; seyyed esmaeil mousavi
Abstract
To study of the effect of biological pre-treatments on germination and growth of Fenugreek (Trigonella foenum-graecum L.) seedling under natural salinity stress, a factorial experiment in a completely randomized design was conducted in three replications. Experimental treatments were biological pre-treated ...
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To study of the effect of biological pre-treatments on germination and growth of Fenugreek (Trigonella foenum-graecum L.) seedling under natural salinity stress, a factorial experiment in a completely randomized design was conducted in three replications. Experimental treatments were biological pre-treated at four levels (control, inoculation with Azoto bio-fertilizer, inoculation with Phosphate bio-fertilizer, inoculation with both bio-fertilizers) and natural salinity (salt of Qom Lake) at five levels (zero, 3, 6, 9 and 12 dS/m).The results showed that increasing of salinity levels led to increasing of mean germination time and using of bio-fertilizer treatments and combination treatment caused this index show 22 percentages reduction at highest level of salinity compared to control treatment at same level of salinity. Salinity reduced the germination coefficient and the highest amount of this index (74.3) obtained at 3 dS/m level of salinity that compared to control treatment and at the same level of salinity showed 89 percentages increasing. Salinity reduced total chlorophyll and using of biological treatments caused amount of total chlorophyll at zero level of salinity show 155 percentages increasing compared to control treatment and at the same level of salinity. With increasing of salinity level increased soluble protein and combination treatment increased this index 12 percentages at the highest level of salinity compared to control. Under stress, plants face with oxidative stress and to confront of that use antioxidant enzymes. In this experiment, the using of biological treatments by raising of the production of antioxidant enzymes caused the reduction of salinity effects.
seyyed esmaeil mousavi; Heshmat Omidi
Abstract
To study the effect of biological treatment on germination and growth indices and pigments of flaxseed under salinity stress, a factorial experiment in a completely randomized design was conducted in three replications at the faculty of agriculture of Shahed University in 2018. Experimental treatments ...
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To study the effect of biological treatment on germination and growth indices and pigments of flaxseed under salinity stress, a factorial experiment in a completely randomized design was conducted in three replications at the faculty of agriculture of Shahed University in 2018. Experimental treatments were salinity at four levels (zero, 2.5, 5 and 7.5 dS/m) and biological treatments at four levels (control, bacteria (Baccillus subtilis), fungi (Macrophomina phaseolina) and combination of bacteria and fungi). Effect of biological treatment was significant on germination percentage and the highest germination percentage (99 %) obtained at fungi treatment. Effect of salinity and biological treatment was significant on mean germination time and germination coefficient. The minimum mean germination time (3.79 days) obtained at combination of bacteria and fungi treatment that shows this treatment was most effective in compared with other biological treatments. With increasing of salinity, mean germination time increased. Salinity reduced radicle, plumule and seedling length. The means related to radicle length at the different levels of salinity at biological treatment of fungi was highest amount in compared with other biological treatments. Interaction effect of salinity and biological treatment was significant on chlorophyll a and total chlorophyll. With increasing of salinity, reduced pigments. Maximum amount of chlorophyll a (16.52 µm.ml1-) was obtained at the control treatment of biological treatment and zero salinity. The result of this research showed that using of microorganisms such as fungi and bacteria in salinity stress, can reduce the negative effects of salinity on germination and growth of this plant.
Milad Razaji; Azra Ataei Azimi; Babak Delnavaz Hashemloian
Abstract
The aim of this study was the evolution of effects of seed coating with starch (amylose) and agar on germination and physiological indexes in alkaline stress. This factorial study was conducted in a randomized block design. Factors included seed coating at three levels (control, agar and starch coat) ...
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The aim of this study was the evolution of effects of seed coating with starch (amylose) and agar on germination and physiological indexes in alkaline stress. This factorial study was conducted in a randomized block design. Factors included seed coating at three levels (control, agar and starch coat) and alkaline stress (the mediums with pH values of 6-10 with sodium carbonate and sodium bicarbonate with values of 0-90 mMol). By sowing seeds for 7 days in petri dishes and 20 days in pots, seed potency, amylase activity, germination rate, growth, minerals and chlorophyll a and b were evaluated. Starch and agar reduced the effect of alkali stress. They increased seed potency (84%), amylase activity (53 mg / min), germination rate (11.32 seeds per day) and growth (23 cm) in medium with pH = 10 with a significant difference.also they prevented the decomposition of chlorophyll and reduces the absorption of minerals. Starch coating was more suitable for sowing seeds under normal conditions and alkaline stress, and agar coating was more suitable for maintaining seed vigor and seedling growth in higher amounts of alkaline.
marzie soltanialikooyi; ali abbasi sourki; mohsen mobini dehkordy; shahram kiani
Abstract
In order to comparison the effects of growth promoting bacteria on germination of hamadani alfalfa seeds, a randomized complete block design was conducted with 4 replications and 8 levels of bacterial inoculation (include non-inoculation, application of Acinetrobacter calcoaceticus PTCC 1318, Bacillus ...
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In order to comparison the effects of growth promoting bacteria on germination of hamadani alfalfa seeds, a randomized complete block design was conducted with 4 replications and 8 levels of bacterial inoculation (include non-inoculation, application of Acinetrobacter calcoaceticus PTCC 1318, Bacillus megaterium PTCC 1250, Enterobacter aerogenes PTCC 1221 lonely and their pairwise and triple combination of them(. Another CRD factorial experiment was also conducted with 3 replications in greenhouse conditions. Mentioned bacterial treatments were as the first factor and injection of bacteria into the soil of pots as the second factor. The results showed that application of bacteria increased the vigor index of seedling, percentage and germination rate, but did not affect other alfalfa traits in the laboratory. Inoculation of seeds with bacteria in the pots had positive effects on alfalfa growth, percentage and rate of emergence, dry weight, root volume and length, chlorophyll a, b and total chlorophyll content. Injection of bacteria to the soil also affected some traits, which could be due to better placement and/or a larger population of bacteria in the rhizosphere. Application of bacteria lonely had better results than their combinations. It may be due to antagonistic effects of bacteria on each other, which may reduce positive effects.
Ameneh Akbarzadeh Sharafi; Hamid Reza Eisvand; N. Akbari; D. Goodarzi
Abstract
In order to study effects of seed priming with Gibberellin (GA), Ascorbic acid (AsA) and Salicylic acid (SA) on mitigation of seed deterioration damage and improvement of seed and seedling qualities of three sunflower cultivars (Farrokh, Barzegar and Shamshiri), a pot experiment was done in 2016. This ...
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In order to study effects of seed priming with Gibberellin (GA), Ascorbic acid (AsA) and Salicylic acid (SA) on mitigation of seed deterioration damage and improvement of seed and seedling qualities of three sunflower cultivars (Farrokh, Barzegar and Shamshiri), a pot experiment was done in 2016. This research was carried out as a factorial experiment based on RCBD with three replications. Seeds were exposed to accelerated aging then primed. Priming treatments were included: control, hydropriming, GA (50 and 75 ppm), AsA (100 and 150 ppm), SA (138 and 207 ppm), GA 50 ppm + AsA 100ppm, GA50 ppm + SA 138 ppm, AsA 100ppm + SA 138ppm, and GA 50ppm + AsA 100ppm + SA 138 ppm. Barzegar cultivar had better seedling emergence percentage and rate than the Shamshiri and Farrokh. Seedling emergence was improved by priming treatments; however, suitable treatments were different for the cultivars. Maximum seedling emergence percentage was obtained in Barzegar by GA 50ppm + SA 138ppm; however in Shamshiri and Farrokh by hydropriming. Priming improved seedling emergence rate of the three cultivars, but the response to priming was cultivar specific. Priming decreased seedling malondialdehyde (MDA) significantly in three cultivars. The lowest MDA content was obtained in Barzegar cultivar primed by AsA + SA.
S. Fallah
Abstract
Poor seedling establishment and total plant biomass reduction in contaminated soils, decrease the efficiency of phytoremediation techniques. In this study, the effects of different types of seed pretreatments (such as salicylic acid, and potassium nitrate) and three concentrations of lead (0, 200, 400 ...
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Poor seedling establishment and total plant biomass reduction in contaminated soils, decrease the efficiency of phytoremediation techniques. In this study, the effects of different types of seed pretreatments (such as salicylic acid, and potassium nitrate) and three concentrations of lead (0, 200, 400 and 600 mg lead/kg soil) on sunflower seedling growth under greenhouse conditions were evaluated. These factorial experiment was conducted in completely randomized design with three replications in research greenhouse of Shahrekord University, in 2015. In general, the studied traits significantly decreased with increasing concentrations of lead. In most toxic concentrations of lead, Potassium nitrate and salicylic acid prevented the decreasing of dry weight of root compared to control up to 42%. Potassium nitrate prevented the decreasing of dry weight of leaf and chlorophyll a (with 24% and 60%, respectively), Salicylic acid prevented the decreasing of chlorophyll b (55%) compared to control. Shoot dry weight didn’t affect by seed priming treatments. Increasing in concentrations of lead elevated carotenoids, proline content and electrolyte leakage. Salicylic acid treatment reduced of electrolyte leakage (by 21%). Potassium nitrate prevents the increasing of carotenoids (by 24%), however increased proline content from 104 to 111 µm/g. Generally it could be concluded that salicylic acid and potassium nitrate increase seedling tolerance to lead toxicity through reducing carotenoids content, electrolyte leakage and increasing proline content. This technique can be used to increase the efficiency of phytoremediation of soils contaminated with lead.
