Bita Oskouei; Leila Sadeghi; Kobra Moslemkhani; Hadis Afshar; Zahra Radmanesh
Abstract
Genetic purity, as one of the important characteristics of the quality of seed lots, has been considered in the national standards of certified seed producing countries to achieve desirable and stable characteristics of agricultural cultivars. According to the national standard, determining the other ...
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Genetic purity, as one of the important characteristics of the quality of seed lots, has been considered in the national standards of certified seed producing countries to achieve desirable and stable characteristics of agricultural cultivars. According to the national standard, determining the other speciese in the seed sample is considered as one of the important tests of seed quality. This feature is checked with an emphasis on recognizable characteristics in the seed analysis laboratory. The research has investigated the characteristics and differences in appearance and genetics of seeds off- type of chickpea seeds. The results showed that the seeds that were considered as off- type morphologically differed from the control in at least one of the measured traits. These seeds were different from the original seed in terms of color, shape seed surface roughness and seed groove, also the selected indices were similar in the first and second generation seeds. The results of the molecular test also confirmed the results of the morphological investigations. In Adel, Mansour, Arman cultivars, the samples that in morphological studies were considered as off-type, in molecular studies using microsatellite markers H3F09, H3C11 and H1A06 showed that the molecular profile was not uniform and allele diversity was greater than the main allele of cultivars. In the process of seed certification program, microsatellite markers are a suitable and complementary tool to the use of morphological markers to determine the genetic purity of chickpea seed samples.
Mehran Sharafizad; Shahla Hashemi Fesharaki
Abstract
This study was carried out based on complete randomized design in four replications in order to assess germination and seedling vigor of barely seeds Cv. Goharan exposed to Zn-micronutrient element and different fungicides. Evaluated treatments on Barely seeds include six fungicide treatments Dividend ...
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This study was carried out based on complete randomized design in four replications in order to assess germination and seedling vigor of barely seeds Cv. Goharan exposed to Zn-micronutrient element and different fungicides. Evaluated treatments on Barely seeds include six fungicide treatments Dividend star (2 per 1000), Carboxin Thiram (2.5 per 1000), Raxil (0.5 per 1000), Lamardor (0.2 per 1000), Rovral-TS (2.5 per 1000) and non-fungicide, and three Zn-seed treatments, Zn-Kavin, Zn-Zagorat and non-Zn seed treatment. Barely seed germination was two percent lower than control compared with Zn-seed treatments. The lengths of radicle (12%), shoot (20%), and seedling (13%) after seeds treated with Zn-seed treatments along with Rovral-TS and Carboxin Thiram were more than the ones were recorded with liquid Dividend star. Other fungicides except liquid Dividend star showed similar response to control about seedling length vigor index. The highest rate germination (R50, 1.20) was belonging to Rovral-TS, Carboxin Thiram, and Raxil fungicides in combination with Zn-seed treatments, Moreover, the lowest time to reach maximum of germination (less than 2 days) was recorded in mentioned treatments. However, two fungicides of liquid Dividend star and liquid Lamardor had lower germination rate (1.01) than control (1.06 till 1.1), so these two fungicides usage with such condition was not suggested in this study. In conclusion, Rovral-TS and Carboxin Thiram along with Kavin and Zagorat Zn-seed treatments were suggested to improve germination and seedling vigor of barely indices.
Reza Rezvani; Behnam Kamkar; Zeinab Jabbari Badkhor
Abstract
To mitigate the detrimental effects of salinity stress on vegetable crops, various approaches, including the applying of organic inputs during different growth stages, such as germination, are employed. This study, conducted in 2023, aimed to assess the influence of humic acid pretreatment on the germination ...
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To mitigate the detrimental effects of salinity stress on vegetable crops, various approaches, including the applying of organic inputs during different growth stages, such as germination, are employed. This study, conducted in 2023, aimed to assess the influence of humic acid pretreatment on the germination characteristics and growth parameters of cucumber (Cucumis sativus) under salt stress. Two experiments, one in the laboratory and the other in a greenhouse, were carried out using a factorial design based on a completely randomized design with three replications. The experimental factors included humic acid priming at four concentrations (0, 50, 100, and 200 mg/l-1) and salinity at five levels (0, 25, 50, 100, and 150 mM NaCl). Results indicated that humic acid exerted a positive impact on various germination and growth attributes of cucumber, including germination percentage, germination rate, seedling length, dry weight, vigor index, and chlorophyll a, b, and carotenoid under salt-stressed conditions compared to the control. The treatment with 200 mmol/l humic acid and no salt exhibited the highest germination rate (87%) and seedling length (12.66 cm). The interaction between salinity and humic acid revealed that humic acid concentrations exceeding 100 mg/l-1 could alleviate the adverse effects of salinity stress on total chlorophyll. In conclusion, the cost-effective and easily applicable method of priming cucumber seeds with humic acid presents a potential strategy to mitigate the impacts of salinity stress in regions with saline water or soil.
