Ali Shayanfar; Bita Oskouei; Abbas Dehshiri; Hadis Afshar; Elahe Gheysari
Abstract
Randomized factorial design was implemented in four replications to determine optimal methods of Ajwain (Carum capticum) standard seeds germination. Ajwain seeds were placed top and between paper and sand exposed to two potassium nitrate (two grams per 1000 ml distilled water) and pre-chilling (seven ...
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Randomized factorial design was implemented in four replications to determine optimal methods of Ajwain (Carum capticum) standard seeds germination. Ajwain seeds were placed top and between paper and sand exposed to two potassium nitrate (two grams per 1000 ml distilled water) and pre-chilling (seven days under 7-10ºC) pre-treatments which were under six temperatures (10, 15, 20, 25, 20-30, and 35ºC) in germinator for 25 days so as to germination indices were assessed. Ajwain seeds viability was estimated 75% after tetrazolium test. The highest final and normal seedling percentages were found under 15 and 20ºC in pre-chilling treatment and also 20ºC in potassium nitrate and control. No germination was observed under 35ºC in potassium nitrate and control, however, pre-chilling led to >26% germination. The higher germination potential was shown in top and between papers compared with sand. The lowest germination rate was recorded in control and potassium nitrate and the highest one in pre-chilling. The maximum of seedling weight vigor was suggested in top and between paper when seeds were put under 10, 15, and 20ºC. In order to evaluate optimal standard germination of Ajwain seeds, it was resulted that applying of two potassium nitrate and pre-chilling treatments placed top and between papers under 15 and 20ºC caused maximum of germination potential and seedling weight vigor.
Simin Haghanifar; Marjan Diyanat; Aidin Hamidi; Fereidon Ghasemkhan ghajar; Elias Soltani
Abstract
This research was done in order to find the most effective treatment to break the hardness of Persian camelthorn (Alhagi camelorum) seeds, which is one of the major problems of its cultivation on a large scale. treatments including 98% sulfuric acid (treatments of 25, 30 and 35 minutes) and 100°C ...
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This research was done in order to find the most effective treatment to break the hardness of Persian camelthorn (Alhagi camelorum) seeds, which is one of the major problems of its cultivation on a large scale. treatments including 98% sulfuric acid (treatments of 25, 30 and 35 minutes) and 100°C hot water (treatments of 30 seconds, 1 and 3 minutes) were performed in comparison with the control. The factorial experiment was conducted in the form of a completely randomized design. The results showed that the populations had significant differences in terms of all traits and the difference between seed hardness treatments and their interaction effects for all traits was significant at the 1% level. The results of the findings show that the presence of the hard shell is a physical barrier and it acts as factors limiting germination by preventing the expansion of the embryo or by limiting water absorption and gas exchange. Therefore, applying a series of seed scratching treatments such as sulfuric acid treatment and 100°C water will improve the germination of the camelthorn seed. Because the use of treatments such as sulfuric acid involves problems such as the risk of working with acid, and also due to the cheapness and availability of water, the lack of special facilities and materials, the harmlessness and simplicity of working with it to stimulate germination and remove hardness, 100 °C water for 30 seconds and placement of seeds in 30 °C germinator is the priority for treating Iranian seeds.
Parimah Shokouhi Nasab; Hamidreza Balouchi; َAli Moradi; Mohsen Movahhedi Dehnavi
Abstract
This experiment was conducted in order to investigate the effect of seed coating with biochar and activated carbon on some quinoa seed germination indicators, and it was implemented as a factorial in the form of a completely randomized design with four replications in 2019, in Yasouj University's Faculty ...
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This experiment was conducted in order to investigate the effect of seed coating with biochar and activated carbon on some quinoa seed germination indicators, and it was implemented as a factorial in the form of a completely randomized design with four replications in 2019, in Yasouj University's Faculty of Agriculture. The first factor is seed coating in four levels (no coating, coating with activated carbon, coating with biochar and coating with activated carbon + biochar) and the second factor includes salinity stress in four levels (zero, 75, 150 and 225 mM sodium chloride). The results of the interaction of salinity stress and seed coating showed that the highest content of soluble sugar (28.578 mg/g seed FW) and malondialdehyde (2.97 µmol/g seed FW) was obtained by coating with activated carbon in 150 mM salinity stress. Also, the highest amount of seed hydrogen peroxide (0.18 μmol/g seed FW) and proline (10.49 μmol/g seed FW) at the level of 225 mM sodium chloride was obtained by covering the seeds with biochar and active carbon, respectively. Covering seeds with activated carbon and biochar led to an increase in the length of root and shoot under salt stress conditions. However, under salinity stress conditions, root weight and length vigor index of seed improved more by coating seeds with activated carbon. It can be stated that seed coating can greatly reduce the harmful effects of osmotic stress on germination and biochemical traits in quinoa seedlings and improve seedling growth.
Ahmadreza Najafi; Asghar Estaji; Mahdi Ghasemi
Abstract
The percentage and rate of seed germination are critical factors leading to high yield in agriculture, but it has a diminishing effect on environmental stress. Seed pretreatment is one of the ways to increase the strength and velocity of seed germination to deal with salt stress. The purpose of this ...
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The percentage and rate of seed germination are critical factors leading to high yield in agriculture, but it has a diminishing effect on environmental stress. Seed pretreatment is one of the ways to increase the strength and velocity of seed germination to deal with salt stress. The purpose of this study was to investigate the effect of seed pretreatment by peppermint (0.5, 1, and 2%) and seaweed extract (0.5, 1, and 2%) with control on the growth and biochemical traits of Thymus daenensis seedling under salinity stress (0, 50, 100, and 150 mM NaCl) conditions. This experiment was conducted as a factorial as a completely randomized design in four replications at the seedling growth stage. The results showed that seed pretreatment with peppermint and seaweed extract increased the percentage, velocity, and time of germination as well as the number of germinated seeds, in the absence of salinity stress. In seedling growth characteristics, although they were better in the absence of salinity stress, the pretreatment of the seeds significantly reduced the effects of salinity stress compared to the control conditions. The highest amount of chlorophyll in this condition was obtained in seeds treated with 2% seaweed extract. The highest activity of catalase enzyme and polyphenol oxidase enzyme was obtained in 100 and 150 mM salinity stress conditions and seeds treated with 1 and 2% seaweed extract, respectively. Salinity stress had caused a decrease in growth parameters, but seaweed extract pretreatment had improved the conditions.
Leyla Cheheltanan; Ali Tehranifar; Mahmoud Shoor; Seyyed Hossein Neamati; Saeed Khosravi
Abstract
Self-pollination induces a phenomenon known as inbreeding depression in heterozygous plants, which in turn leads to a reduction in seed germination and seedling growth. On the other hand, seed priming with plant hormones such as gibberellic acid is a technical approach that potentially results in the ...
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Self-pollination induces a phenomenon known as inbreeding depression in heterozygous plants, which in turn leads to a reduction in seed germination and seedling growth. On the other hand, seed priming with plant hormones such as gibberellic acid is a technical approach that potentially results in the improves of rapid and continuous seed germination and subsequent plant growth. Therefore, this research aimed to investigate the effect of different concentrations of gibberellic acid on the improvement of seed germination resulting from self-pollination in various varieties of Petunia hybrida in a factorial experiment with three replications as a completely randomized design. The treatments consisted of four levels of gibberellic acid (0, 50, 100, and 150 mg/L) and seeds obtained from self-pollination of Iranian Petunia, Tango Blue, Tritunia Pink Morn, and Tritunia White. The results indicated that seed priming with gibberellic acid, especially at a concentration of 100 mg/L, led to a significant increase in the activity of α-amylase and β-amylase by 6.56% and 1.57%, respectively. This increase resulted in 8.54% rise in soluble sugars content, providing energy that significantly enhanced germination percentage, germination speed, average time required for germination, seed vigor index, germination energy, and the fresh and dry weight of plumule and radicle. Moreover, among different varieties, seeds obtained from self-pollination of Iranian petunia demonstrated a higher germination percentage and germination speed compared to seeds from other varieties, indicating a superior capability in maintaining vigor potential, germination percentage, and germination speed.
Hamid Reza Darafshi; Seyyed Mohammad Javad Arvin; Fatemeh Nejad-Alimoradi
Abstract
Salicylic acid (SA) plays a crucial role in regulating various physiological mechanisms, including; germination, growth, flowering and fruit ripening. Here, the impact of seed priming of tomato (Tina) with SA on indicators of seed germination, seedling growth and quantitative and qualitative characteristics ...
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Salicylic acid (SA) plays a crucial role in regulating various physiological mechanisms, including; germination, growth, flowering and fruit ripening. Here, the impact of seed priming of tomato (Tina) with SA on indicators of seed germination, seedling growth and quantitative and qualitative characteristics of the fruit was examined under field conditions. The treatments included seed priming in SA solution with zero, 0.10, 0.25 and 0.50 mM for a duration of 24 h. Then, the early seedlings obtained from SA priming (0.25 and 0.50 mM) were grown in the greenhouse and transferred to the field. The results showed that SA led to the significant increase in seed germination properties, also an increase in all the traits of the seedlings, the effect of 0.50 mM was more significant, so that compared to the control, this concentration increased the seed germination percentage and speed (16% and 28 % respectively) and the dry weight of seedling shoot (124%). Moreover, SA contributed to the enhancement of measured field traits and the qualitative of fruit extract, such as carotenoid, ascorbic acid levels, acidity and total soluble solids, and for all traits, except for the number and weight of fruit per plant, no difference was observed between the two concentrations of SA. The highest number and weight of fruit was obtained from the 0.50 mM treatment and the fruit yield increased by 75% compared to the control. Overall, due to the small consumption of chemicals and the ease of used, SA priming will help to reduce production costs.
Volume 1, Issue 2 , December 2012, , Pages 117-127
Abstract
Increased salt tolerance is essential need for sustainable barley production in areas with risk of salinisation. This requires new genetic sources of salt tolerance, and more efficient techniques for identifying salt-tolerant germplasms. This study, conduct to access salt tolerance in primary growth ...
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Increased salt tolerance is essential need for sustainable barley production in areas with risk of salinisation. This requires new genetic sources of salt tolerance, and more efficient techniques for identifying salt-tolerant germplasms. This study, conduct to access salt tolerance in primary growth stage of eight barley cultivars and find the most beneficial salt tolerance traits in this stage. The cultivars were studied at Non-Stress (Normal) and salinity stress (24 dS/m) condition with four replication. Results of ANOVA showed significant differences among cultivars and condition for most traits, which demonstrates high diversity among combinations that enabled us to screen salinity tolerant cultivar. Estimation of coefficient of genotypic variation (GCV) showed less than estimate of coefficient phenotypic variation (PCV) under salinity stress condition for most traits. Also germination prcentage under salinity stress condition had high GCV and h2and suggested that there is high importance of additive gene action for seed vigority. So using these traits as selection index can be effective. Cluster analysis with Wardandrsquo;s method for both condition classified hybrids into three different groups. According to the results of this research, genotypes Salt Toleranc,EB-81-15,NomarandNimrozidentified as tolerant lines cultivars to salinity at germination stage and early seedling growth.
Volume 2, Issue 2 , December 2013, , Pages 127-134
Abstract
The aim of the this study was to investigate the allelopathic effect of aerial and underground tissues of mountain sagebrush (Artemisia aucheri) on seed germination properties, seedling length, number of leaves, seedling dry weight and seed vigor of Festuca arundinaseae species. For this purpose, ...
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The aim of the this study was to investigate the allelopathic effect of aerial and underground tissues of mountain sagebrush (Artemisia aucheri) on seed germination properties, seedling length, number of leaves, seedling dry weight and seed vigor of Festuca arundinaseae species. For this purpose, species of Ar. aucheri selected from Taleghan rangelands in Tehran Province. Then for 72 hours aerial and underground issues were exposed to direct sunlight. After air drying, obtained powder was used to do the experiment. The provided powder of 3, 6 and 9 grams from aerial and underground tissues of sagebrush mountion (Ar. aucheri) was mixed with 900 grams of sand in pots and arranged in a completely randomized with four replications. 10 seeds from Festucaarundinaceae were planted in each pot to 2-3 cm depth and germination percentage, germination rate, mean germination time, seeding length, leaf number, seedling weight and seed vigor was measured. Result showed that above ground and underground parts of Ar. Aucheri reducted germination and vigor characteristics of Festuca arundinaseae. Comparing the influence of aboveground and underground tissues revealed that root effectiveness was more than shoot.Regardless of the type of Artemisia effective compounds, characteristics of this plant on seed germination and early growth of seedlings Festucaarundinaceae have adverse effects that can be considered seriously in developing culture program of these two plants.
Volume 3, Issue 2 , December 2014, , Pages 137-151
Abstract
During seed storage, some seeds traits might change and seed vigor may decrease in bad storage situation, to determine the effect of some hormones in different concentrations, the study was conducted in biotechnology laboratory of Agriculture University of Tehran in 2012. In this study, seeds aging were ...
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During seed storage, some seeds traits might change and seed vigor may decrease in bad storage situation, to determine the effect of some hormones in different concentrations, the study was conducted in biotechnology laboratory of Agriculture University of Tehran in 2012. In this study, seeds aging were tested for 0, 2 and 4 days and after they were primed with distilled water, salicylic acid, IAA and kinetin. Traits measured included germination percent, germination rate, shoot length, root length, seedling dry matter and vigor. The results showed that hydro priming improved many characteristics in fresh seeds but it's not very useful for seeds under ageing conditions. For rising seed vigor in these cases, it's better to use hormones, especially salicylic acid which is called tolerant hormone. Using IAA because of broadleaf control property, it doesn’t seem logically. The best treatment in this study was salicylic acid in low concentration
Abstract
Editorial, Tenth anniversary of Iranian Journal of Seed Science and Technology
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Editorial, Tenth anniversary of Iranian Journal of Seed Science and Technology
Mehrnoosh Jadidi; Asa Ebrahimi
Abstract
Replacing biological herbicides instead of chemical herbicides has not only had adverse environmental effects but also has the advantage of biodegradability. In this study, the effect of rye extract in biocompatible polymer chitosan / alginate nanocapsule with non nonocapsulated extract on dodder and ...
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Replacing biological herbicides instead of chemical herbicides has not only had adverse environmental effects but also has the advantage of biodegradability. In this study, the effect of rye extract in biocompatible polymer chitosan / alginate nanocapsule with non nonocapsulated extract on dodder and purslane were investigated. The experiment was conducted in a completely randomized design with factorial arrangement. Extracts of shoot and root were evaluated at different concentrations (0, 6.25, 12.5, 25, 50 and 100%) on germination percentage, germination rate, radicle and plumule length, radicle and plumule fresh weight. The results showed that the particles were relatively spherical with a smooth surface and the shoot and root extracts had particle sizes of 113 and 350 nm and 71.4 to 129 nm, respectively. Nano-extract of shoot and root reduced germination of sauces seed to zero at concentrations of 25, 50 and 100%. The highest germination percentage of purslane seed belonged to control treatment (78.26%) which was significantly different from other treatments. Shoot and root nano extracts caused complete inhibition of purslane seed germination at concentrations of 25, 50 and 100%, which were significantly different from other treatments. Increasing concentration of rye extract increased the inhibition percentage of studied traits and nanocapsulation increased allelopathic effect of rye extract.
Volume 2, Issue 1 , March 2013, , Pages 9-24
Abstract
In order to evaluate the effect of seed viability (germinability) on seedling field, seed cotton yielde and some related traits of commercial cotton cultivars, an experiment was conducted in 2008 and 2009 at Seed and Plant Certification and Registration seed analysis laboratory and research field at ...
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In order to evaluate the effect of seed viability (germinability) on seedling field, seed cotton yielde and some related traits of commercial cotton cultivars, an experiment was conducted in 2008 and 2009 at Seed and Plant Certification and Registration seed analysis laboratory and research field at Karaj. Seeds of Mehr, Varamin, Sahel and Bakhtegancotton cultivars produced in 2007 and 2008 with 70, 80 and 90 percent germinability tested by standard germination test in laboratory. Seeds planted in field as two factor factorial experiment by 12 treatments(4 cultivarandtimes;3 seed viability) based on complete randomized block design by four replicates. Then 19 traits including primary field emergence(PFE), final field emergence(FFE), field emergence rate(FER), cumulative emergence rate(CER), mean emergence time(MET), field emergence index(FEI), seedling primary height(SH1), seedling height in second sampling(SH2),seedling height in third sampling(SH3), seedling final height(SH4), primary seedling field vigour index(PSVIF) and final seedling field vigour index(FSVIF), vegetative and reproductive branches number, final plant height, boll number and seedcotton yield per hectare determined. The results indicatedthat over standard viability seeds had high field emergence index(FEI), seedling emergence rate(SER) and primary and final field emergence(FFE) and seedling height andprimary and final seedling vigoure index in field and therefore had better field emergence and establishment. Also seeds have over standard viability had lower plant final height and vegetative branches which stated those lower vegetative growth and had more reproductive branches, boll number and weight per plant and resultant seed cotton yield per hectare. Thereafter in order to reduce seed consumption rate to recommended amount generally based on this experiment results planting seeds have90 percent germination recommended.
F. Morsali Aghajari; R. Darvishzadeh; H. Hatami Maleki
Volume 5, Issue 2 , January 2016, , Pages 9-22
Abstract
Salt stress is one of the most serious limiting factors for crop growth and production. In order to study the effect of salinity stress on the percentage and rate of germination of sunflower seeds and localization of genes controlling the traits in sunflower genome, a factorial experiment in a completely ...
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Salt stress is one of the most serious limiting factors for crop growth and production. In order to study the effect of salinity stress on the percentage and rate of germination of sunflower seeds and localization of genes controlling the traits in sunflower genome, a factorial experiment in a completely randomized design with three replications were conducted in Department of Plant Breeding and Biotechnology, Urmia University. The first factor was 70 recombinant inbred lines (RILs) of sunflower derived from the cross PAC2 (female parent) × RHA266 (male parent) together with parents and the second factor was salinity stress levels including 0, 2, 4, 6 and 8 dS/m. The results of analysis of variance revealed statistically significant differences among RILs and salinity levels based on two studied traits in 1% probability level and also significant interaction of Line by Salt stress. Heritability for germination rate and percentage was estimated 20% and 14%, respectively. Mapping of genes was performed using constructed SSR/SNP linkage map including 221 markers with average distance of 7.44 CM between markers. In several salinity levels, 22 QTLs for germination rate and 12 QTLs for germination percentage were detected. The effects of detected QTLs were varied between 2.04% to 67.61%. In this study, QTLs including salt2.PGS.13.1, salt2.PGS.2.1, salt2.GR.1.1, salt4.GR.9.1, salt6.GR.10.1 and salt8.GR.6.1 exhibited maximum phenotypic coefficient of variation. The results of this study revealed some non-specific QTLs (co-localized) for studied charactristics which are valuable in plant breeding programs and make it possible to have simultaneous selection for several charactristics.
R. Ostadian Bidgoly; H.R. Balouchi; E. Soltani; A. Moradi
Abstract
There is a little information about the effects of temperature and water potential on safflower. In order to study the effects of temperature and water potential on seed germination characteristics of safflower (Carthamus tinctorius L.), an experiment as combined analysis was conducted base on a completely ...
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There is a little information about the effects of temperature and water potential on safflower. In order to study the effects of temperature and water potential on seed germination characteristics of safflower (Carthamus tinctorius L.), an experiment as combined analysis was conducted base on a completely randomized design with 4 replications by 25 seeds in laboratory of seed technology at Yasouj University in 2015. The factors included temperature (5, 10, 15, 20, 25, 30, 35, 40 and 45 oC) and water potential (0, -0.2, -0.4, -0.6, -0.8, -1, -1.2, -1.4 and -1.6 MPa). The percentage and rate of germination, root length, shoot length, ratio of root length to shoot length and seedling vigor index were measured in this experiment. The results of analysis of variance showed that main effects of temperature and water potential and interactions were significant on all indicators of germination at 1% probability level. Also, by decreased water potential, germination percentage and germination rate reduced. At 5 oC and at zero potential germination percentage was 98% while the potential of -1.6 MPa germination percentage dropped to zero. At 35 oC and at zero potential germination percentage was 95% while the using potential of -1.6 MPa germination percentage decreased to 1%. By increasing the temperature up to 20oC increasing germination percentage and germination rate detected and afterwards reduced.
Abstract
In order to investigate the effect of seed bio-inoculation on some germination indices of Cumin under drought stress a two factors experiment was laid out. Experimental factors included priming in nine levels (bio- inoculation with 4 strains of PF1, PF2, PF75 and CHA0 of Pseudomonas fluorescent, and ...
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In order to investigate the effect of seed bio-inoculation on some germination indices of Cumin under drought stress a two factors experiment was laid out. Experimental factors included priming in nine levels (bio- inoculation with 4 strains of PF1, PF2, PF75 and CHA0 of Pseudomonas fluorescent, and 4 strains T29, T36, T39 and T40 of Trichoderma harzianum and non-primed (control)) and three levels of drought stress (0, -3 and -6 bar imposed by PEG 6000). Results showed that by increasing the osmotic potential levels germination indices decreased. At all levels of stress, inoculated seeds had higher germination percentage and root length than non-inoculated seeds. In under stress level of -3 bar, highest germination percentage (86.66%) and seedling weight vigor index (11.93) was obtained from T36 strain that; this treatment was able increase germination by more than 35% than non-inoculated seed. Under -6 bar, the highest (76.68%) and lowest (55%) germination percentage was related to T29 that has no significant difference with T36 (75.83%) and T39 (75.83%) and the lowest amount was related to non-primed treatment (55%). Also at all stress level, the highest length seedling vigor index was related to CHA0 strain. The highest weight seedling vigor index was related to T36 strain (6.4, 5.49 and 4.7 respectively in 0, -3 and -6 abr) and the lowest (4.89) was related to non-inoculated (control) seeds. According to the results, T36 can be suggested as a suitable pretreatment in moderation of the negative effects of osmotic potential on germination of cumin seeds.
Abstract
One of the major factors for the low productivity of seeds is poor seed germination and early seedling mortality due to seed-borne fungi. Several grain-borne fungi, including species of Alternaria, Aspergillus, Fusarium and Penicillium, have been implicated as seedling pathogens of cereals. Experiments ...
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One of the major factors for the low productivity of seeds is poor seed germination and early seedling mortality due to seed-borne fungi. Several grain-borne fungi, including species of Alternaria, Aspergillus, Fusarium and Penicillium, have been implicated as seedling pathogens of cereals. Experiments were conducted at the seed and Plant certification and Registration Research Institute. Experimental units were arranged factorial in a completely randomized design with two factors (cultivar and storage temperature). The result indicated that interaction between storage temperature × cultivar had significantly affected on seed-borne fungi after three months storage. The seed-borne fungi infestation sach as Asporgilau flavus and Penicillium decreased in all seeds that stored at 20-30C° (alternative temperature), also the Fusarium species infestation was reduced in Usef, Nosrat, and Productive Cultivars seed under storage at 20-30C°, whereas Alternaria alternate infestation was increased in all seeds that stored at 20-30 C° . Meanwhile, normal seedling percentage and seedling vigor were significantly improved by storage seeds at 20-30 C° that it was more pronounced in Sahra and Productive cultivars. Final germination percentage and normal seedling percentage increased, when seed-borne fungi infestation such as Aspergillus flavus,Penicillium sppand Fusarium spp decreased in seed samples. Generally, according to cultivars that examined in this experiment, seed stored at 20-30 C°(alternative temperature) could have maintained their vigour high, it is new founding that could help to control seed-borne fungi (Asporgilau flavus, Penicilliumsppand Fusarium spp) in storage condition also prevent of seed deterioration.
maryam najafian; Ghasem tohidloo; A. Hamidi; farzad paknejad; mehdi rezaei
Abstract
In order to evaluate the genetic purity and originality of three common commercial cotton cultivars (Varamin, Khordad and Bakhtegan) using morphological characteristics and microsatellite markers, the seeds of these cultivars along with pure lines in the field in a two-year randomized complete block ...
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In order to evaluate the genetic purity and originality of three common commercial cotton cultivars (Varamin, Khordad and Bakhtegan) using morphological characteristics and microsatellite markers, the seeds of these cultivars along with pure lines in the field in a two-year randomized complete block design with four replications two methods of field growth test (GOT) and microsatellite markers were tested. The results of the GOT test showed that the pure samples of the cultivars were different from the off-type and non-pure samples in 7 morphological characteristics, and these traits, which are not influenced by the environment, can be used as a key to identify the desired cultivar. In the molecular test, out of 7 markers, 3 pairs were selected due to their resolution and the number of effective alleles (DPL431, DPL0513, and CIR246). Due to the similarity of the results of GOT methods and microsatellite markers and due to the high cost of the molecular method, this method can be used as a quick, case-by-case and complementary method to determine the genetic purity percentage of cultivars.
R. Seyed Sharifi; F. Lotfollah; M. Sedghi; R. Seyed Sharifi
Volume 5, Issue 1 , March 2015, , Pages 15-26
Abstract
In order to investigate the effects of free living nitrogen fixing bacteria application and time of nitrogen spraying on contribution of stem reserves in grain yield, rate and effective grain filling period of Triticale, a factorial experiment was conducted based on a randomized complete block design ...
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In order to investigate the effects of free living nitrogen fixing bacteria application and time of nitrogen spraying on contribution of stem reserves in grain yield, rate and effective grain filling period of Triticale, a factorial experiment was conducted based on a randomized complete block design with three replications in Research farm of the Faculty of Agriculture, University of Mohaghegh Ardabili in 2012. Treatments consisted of different nitrogen spraying times in four levels (no spraying as control, spraying in boot stage, ear emergence, grain filling period) and seed inoculation with plant growth promoting rhizobacteria in four levels containing (without inoculation as control, seed inoculation with Azotobacter chroococcum strain 5, Azospirillum lipoferum strain OF, Psedomunas putida strain 9). Results indicated that spraying time of nitrogen fertilizer and seed inoculation with free living nitrogen fixing bacteria had significant effects on yield, yield components, rate and effective grain filling period of Triticale. Maximum of grain weight (0/054 gr) and effective grain filling period (34.17 days) were obtained in seed inoculation with Azotobacter× nitrogen spraying in boot stage. Maximum of contribution of stem reserves in grain yield (30.63 %) was obtained in no nitrogen spraying × no seed inoculation with PGPR and minimum of it (8.12 and 8.13 %) were obtained in nitrogen spraying in boot stage × seed inoculation with Azotobacter and spraying in ear emergence × seed inoculation with Azotobacter. It seems that in order to increase the grain yield,rate and effective grain filling period of Triticale, it can be suggested that nitrogen spraying is applied in boot stage and seed inoculation with Azotobacter
Volume 4, Issue 1 , March 2015, , Pages 15-26
Abstract
In order to evaluate the priming effects of gibberllic acid and potassium nitrate on germination and seedling growth characteristics of three populations Satureja sahendica،, an experiment was conducted. The treatments including plant populations (Majarition, Zeninjab and Osko), gibberllic acid (500, ...
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In order to evaluate the priming effects of gibberllic acid and potassium nitrate on germination and seedling growth characteristics of three populations Satureja sahendica،, an experiment was conducted. The treatments including plant populations (Majarition, Zeninjab and Osko), gibberllic acid (500, 250 ppm) and potasium nitrate (1%, 2%) and cold treatment in three replications arranged in factorial was carried out in greenhouse and laboratory conditions in Natural Gene Bank Group of Research Institute of Rangeland and Forestry in 2011- 2012. After seedling growth, traits including: percentage and speed of germination, root and shoot length , ratio of root length by shoot length, vigor index, normal seedlings, seedling length, fresh and dry weight and ratio of dry weight to fresh weight were measured in laboratory condition. Also in greenhouse, traits including: percentage and speed of emergence, root and shoot length , ratio of root length by shoot length, normal seedlings, seedling length, fresh and dry weight and ratio of dry weight to fresh weight were measured. Analysis of variance showed that there were significanc differences between populations, treatments and interaction of populations with treatments( 5% ) for all traits in both experimental conditions except for seed emergence percentage and shoot length in greenhouse condition. Comparing seed germination characteristics of three population showed that population of Mjarishen selected from East Azarbijan had higher vigor characteristics like seed germination (97%), speed of germination (15 sprout/day) and vigor index(41) than other populations in laboratory condition. In greenhouse condition, percentage and speed of emergence of Osko population as 17% and 1 sprout/day, respectively, were higher than other two populations. Effect of treatment on the populations showed that priming of the seeds with gibberllic acid were more effective for vigor traits like(germination speed, emergence speed and vigor index) than the other treatments in both experimental conditions.
Zahra Radmanesh; Samad Mobasser
Abstract
This study was aimed to determine the effect of seed size of the first (main) ear and the second ear and drying methods on seed quality of maize hybrid Maxima. For this purpose, two different experiments, including seed production field and laboratory experiment were conducted in 2014 and 2015, in Karaj. ...
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This study was aimed to determine the effect of seed size of the first (main) ear and the second ear and drying methods on seed quality of maize hybrid Maxima. For this purpose, two different experiments, including seed production field and laboratory experiment were conducted in 2014 and 2015, in Karaj. Six rows of maternal line and two rows of paternal line were planted in seed producing field in Shahid Motahari research Station in Sugar Beet Seed research Institute. The second experiment was conducted in qualified seed testing laboratory in SPCRI. The treatments composed of two levels of seed size (7mm and 6mm), two ears position (the first (main) and the second) and two drying methods (air drying and artificial drying). Results showed that the interaction of ear position, seed size and drying methods was significant for GP, GMD, Allometric index, SL, RWW, HW and Alpha-amylase activity. Maximum and minimum of seedling length was 34.48 cm and 30.42 cm respectively and related to seed size of 7 mm of the main ear. Moreover, maximum of germination percentage was 100% that related to seed size of 7mm and 6mm of the first ear which dried by dryer, while minimum value was 83% that related to seed size of 6mm of air dried ears. According to the results of this experiment it seems that seeds of maize hybrid Maxima of 7 mm that were dried by dryer machine because of priority of germination traits are more suitable for cultivation.
Maryam Zamanian; Reza Tavakkol afshari; Jafar Nabati; Alireza Seifi
Abstract
This study was conducted as a factorial experiments based on a completely randomized design with two factors cultivars (Modena and Okapi), aging level (0, 24 and 72 hours at temperature of 40˚c and relative humidity of saturated) and four replications to evaluate germination rate and percentage (GP), ...
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This study was conducted as a factorial experiments based on a completely randomized design with two factors cultivars (Modena and Okapi), aging level (0, 24 and 72 hours at temperature of 40˚c and relative humidity of saturated) and four replications to evaluate germination rate and percentage (GP), vigor index (SV), electrical conductivity (EC), catalase (CAT), ascorbate peroxides (APX), glutathione reductase (GR) and S-nitrosothiol (SNO) at the Ferdowsi University of Mashhad, 2019-20. Accelerated Aging (AA) treatments made significant decline in germination indices and biochemical characteristics (except EC) of canola seeds. Also, Modena cultivar was stronger in germination traits while Okapi cultivar was better in biochemical activities, which can be explained by difference in the primary genetic of the cultivars. According to the results, in the treatments of 72 hours of AA compared to the control, GP has decreased by 86%, CAT 88%, APX 89% and SNOs 36%. On the top of that, there was a positive correlation between SNOs and enzymes. In conclusion, Nitric oxide (NO) is likely to indirectly affect the antioxidant enzymes by SNOs in aged canola seeds. Seed aging targets the cell physiology by reducing the activities of antioxidant enzymes and NO and therefore increasing the rate of cell mortality.
Mehdi Afrouz; Parisa Sheikhzadeh
Abstract
In order to evaluate the effect of Ag synthesized from fennel on seed germination, growth and biochemical characteristics of hybrid single cross 704 corn seedlings, an experiment was carried out in a randomized complete block design with three replications at the University of Mohaghegh Ardabili in 2021. ...
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In order to evaluate the effect of Ag synthesized from fennel on seed germination, growth and biochemical characteristics of hybrid single cross 704 corn seedlings, an experiment was carried out in a randomized complete block design with three replications at the University of Mohaghegh Ardabili in 2021. Experimental factors included synthesized Ag nanoparticles (0, 0.001, 0.1, 0.25, and 0.75 mg L-1) and the application methods of Ag nanoparticles (seed priming and no priming). The results showed that in both methods of nanoparticle application, with the use of different concentrations of Ag nanoparticles, there was a significant increase in the percentage and speed of germination, average daily germination, germination simultaneity index, strength index, length and dry weight of corn seedlings and a decrease in the average germination time, D50 of corn seeds were germinated. Among the nanoparticle application methods, priming seeds with 0.001 mg L-1 and adding 0.1 mg L-1 Ag nanoparticles had a greater effect on improving germination, seedling growth, and increasing seed vigor index. By adding 0.1 mg L-1 of Ag nanoparticles to no priming, it resulted in the highest germination%, synchronicity index, mean daily germination and the lowest mean germination time. The use of different concentrations of Ag nanoparticles in both application methods increased the activity of catalase, peroxidase, polyphenol oxidase and proline content compared to the control treatment, the use of a concentration of 0.001 mg L-1 of Ag nanoparticles as a priming can be suggested to improve seed germination, growth, and biochemical characteristics of corn seedlings.
Akram Rostamipour; Reza Tavakkol Afshari; Hamidreza Khazaie; Danial Kahrizi; Hamid Reza Eisvand
Abstract
Camelina, an oilseed plant in the Brassicaceae family, is extensively utilized in the food and pharmaceutical industries. This study aimed to investigate the effects of micronutrient foliar application on the growth and yield traits of two camelina genotypes (Soheil cultivar and Line-69) during the periods ...
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Camelina, an oilseed plant in the Brassicaceae family, is extensively utilized in the food and pharmaceutical industries. This study aimed to investigate the effects of micronutrient foliar application on the growth and yield traits of two camelina genotypes (Soheil cultivar and Line-69) during the periods 1400-1401 and 1401-1402 at the Agriculture Research Station of Ferdowsi University of Mashhad,, Iran. The experiment was conducted as a factorial design based on a completely randomized block with three replication, considering line and cultivar. The applied treatments included foliar application of iron sulfate, zinc sulfate, manganese sulfate, a mixture of micronutrients (iron sulfate, zinc sulfate, manganese sulfate), distilled water, and a control. These treatments were applied at two stages: 50% flowering and 50% pod formation. The experiment was conducted as a factorial design based on a completely randomized block with three replication, considering line and cultivar. The results of mean comparison revealed that, over two cropping years, the highest 1000-grain weight (1.20 grams) was attained in the Soheil cultivar during the 50% flowering stage in the first crop year, following the application of manganese sulfate via foliar application. The highest number of pods per plant (214 pods) was also observed in the Soheil cultivar during the 50% flowering stage, achieved through foliar application of iron sulfate in the second crop year. Under the influence of cultivar, the highest seed yield per hectare was obtained in the Soheil cultivar (1930 kg/hectare).
A. Avishi; H. Feizi; M. Dashti
Abstract
To evaluate seed vigor of marigold affected as organic, biological, nano and chemical fertilizers on mother plant, a study was conducted in Research Center and Education of Agricultural and Natural Resources of Khorasan Razavi province. Plants were under the impact of 10 fertilizer treatments contains ...
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To evaluate seed vigor of marigold affected as organic, biological, nano and chemical fertilizers on mother plant, a study was conducted in Research Center and Education of Agricultural and Natural Resources of Khorasan Razavi province. Plants were under the impact of 10 fertilizer treatments contains Compost (15 ton/ha), Vermicompost (5 ton/ha), cow manure (15 ton/ha), Compost with Pseudomonas putida, Vermi compost with Pseudomonas putida, Nanobiologic fertilizer (1.5 L/ha), Nano chalate ZFM (2 kg/1000 L), Nano composit Bioorganic (200 kg/ha), Chemical fertilizer (150N,50P,50K) and control. The experiment was conducted in a completely randomized design with 10 treatment with three replications. . Then traits such as percentage and speed of germination, mean germination time, seedling length, seed vigor index and seedling dry weight were measured. The results showed that fertilizer treatments had significant effects on germination percentage, germination rate, mean germination time, seedling length and vigor index of Calendula officinalis seeds but had not on seedling dry weight. The highest seed germination was found in compost treatment that was 94% (24.5% increment) and the lowest was seen in control treatment (76%). The highest germination rate was seen in compost treatment (6.05) and the lowest was in control (2.54). Application of compost in comparison to control showed 46.06% reduction in mean germination time. Also using compost had the greatest germination rate in compared to control (more than 138%). Overall compost treatment showed the best performance on the seed and seedling traits of marigold.
Volume 3, Issue 1 , March 2014, , Pages 17-30
Abstract
In order to identify the accelerated aging effects on enzyme and lipid peroxidation in soybean an experiment was conducted based on completely randomized design by factorial arrangement with five levels of seed aging (3, 6, 9, 12, days to aging) and without aging, and two cultivars (Katol and Sahar) ...
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In order to identify the accelerated aging effects on enzyme and lipid peroxidation in soybean an experiment was conducted based on completely randomized design by factorial arrangement with five levels of seed aging (3, 6, 9, 12, days to aging) and without aging, and two cultivars (Katol and Sahar) with three replications in seed testing Lab of Agricultural and Natural Research Center of Golestan. Accelerated aging was achieved by incubating the seed in a closed plastic box at 40 degree centigrade and close to 100% relative humidity for up to 12 days. Seed viability, electrical conductivity, lipid peroxidation, superoxidedismutase, catalase, peroxidase, polyphenol oxidase, Ascorbate peroxidase activity were measured in aged and non-aged seed. The result indicated that antioxidant enzyme activities except peroxidase were reduced and it causedhigher malondialdehyde (MDA) and electrolyte leakage (EC). All of this effect caused lower seed vigor during aging. There was significant correlation between EC with percentage of seed germination and showed EC could be considered as a rapid method for evaluation of aged seed. Results showed that Katol cultivar was more tolerant than Sahar cultivar to seed aging.
