Rozita Kabiri; Ali Hatami; Hakimeh Oloumi; Mehdi Naghizadeh; Fatemeh Nasibi; Zahra Tahmasebi
Abstract
To evaluate the effect of melatonin pretreatment on germination, early seedling growth and some physiological characteristics of Dracocephalum moldavica under osmotic stress, an experiment was conducted in a factorial arrangement based on completely randomized design with 25 treatments and three replications. ...
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To evaluate the effect of melatonin pretreatment on germination, early seedling growth and some physiological characteristics of Dracocephalum moldavica under osmotic stress, an experiment was conducted in a factorial arrangement based on completely randomized design with 25 treatments and three replications. The experimental treatments were including different concentrations of melatonin (0, 5, 10, 50 and 100 µmol) and osmotic stress (0, -0.2, -0.4, -0.6 and -0.8 MPa). The seeds of moldavian balm which were primed with different concentrations of melatonin for 24 hours, then in order to germination test under osmotic stress, they transferred into sterile petri dishes which contain 10 ml PEG. Results showed that increasing in stress caused reducing of germination percentage and rate, seedling dry weight, seed vigor index, radicle length, radicle dry weight, shoot length, shoot dry weight, photosynthetic pigments, protein content ,increasing and soluble sugar content. Seed priming with melatonin caused an increase of plant tolerance to osmotic stress. Melatonin caused the increment of germination percentage and rate of germination, seed vigor index and ultimately enhanced dry weight of moldavian balm seedlings under stress condition. The seeds which were pretreated with higher concentrations of melatonin (50 and 100 µmol) showed an increasing of physiological characteristics (photosynthetic pigments, soluble sugar content and protein) under stress condition compared to control. No germination was observed in seeds which were pretreated with distilled water at the levels of -0.6 and -0.8 MPa, while the concentrations of 50 and 100 µmol melatonin caused a seed germination at all levels of osmotic stress.
malihe sadeghizadeh; arefe movahedi; pegah pahlavani; razieh azadi; mahmod eslami; Hadi Salek Mearaji
Abstract
Seed germination is a critical stage in plant life. Salinity is one of the most important abiotic stresses in plants that makes it difficult for the germination stage of many plants. In order to investigate the effect of priming seeds of basil (Genovese cv.) with amino acid on germination characteristics ...
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Seed germination is a critical stage in plant life. Salinity is one of the most important abiotic stresses in plants that makes it difficult for the germination stage of many plants. In order to investigate the effect of priming seeds of basil (Genovese cv.) with amino acid on germination characteristics of seeds under salinity stress, an experiment was conducted a factorial based on a completely randomized design (CRD) with four replications in the physiology and medicinal plants laboratory of the Faculty of Dr. Shariati Technical and Vocational College of Tehran (TVU). The Experimental treatments included levels of salinity (0, 3, 6 and 9 ds/m) and priming of seed basil with amino acid (L-cysteine) at three concentrations of 0, 100 and 200 µM. Salinity stress had a significant and decreasing effect on all measures of traits. Priming of seeds with amino acid had a significant effect on all investigated traits except coefficient of allometry (CA) and reduced the effects of salinity stress. Germination percentage did not decrease significantly up to, salinity of 3 dS/m, but at 6 and 9 dS/m salinity reduced by 8 and 41%, respectively, compared with control treatment. Amino acid increased the seed germination rate up to 100 µM at 6 dS/m of salinity, but it was not effective at 9 dS/m. Seed priming with 200 µM amino acid had the highest length of radicle (28.2 mm), which was 19% higher than the control treatment.
P. Bayat; M. Ghobadi; M.E. Ghobadi; Gh.R. Mohammadi
Volume 5, Issue 1 , March 2015, , Pages 27-38
Abstract
ABSTRACT In order to study the ability of standard germination test to predict emergence and establishment of chickpea seedlings in field, an experiment was conducted under two conditions using thirteen seed lots of chickpea in laboratory and research field at the College of Agriculture and Natural Resources, ...
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ABSTRACT In order to study the ability of standard germination test to predict emergence and establishment of chickpea seedlings in field, an experiment was conducted under two conditions using thirteen seed lots of chickpea in laboratory and research field at the College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran, in 2012. In laboratory, thirteen seed lots of chickpea belonged to seven cultivars were planted as a randomized complete design with four replications using International Seed Testing Association methods. In this test, different indices related to seed and seedling vigor were evaluated. These seed lots were also planted under field condition in a randomized complete block design with four replications and percentage and rate of seedling emergence were evaluated. The results of analysis of variance in the laboratory test showed that seed lots differed significantly in final germination percentage, strong seedling percentage, abnormal seedling percentage, not-germinated seeds percentage, mean time germination, germination rate, germination index, seedling vigor index. Comparison of laboratory and field experiments showed that the new seed lots had higher germination characteristics than the old seed lots. Correlation between measured traits in standard germination test under laboratory condition with field condition showed that the percentage and rate of seedling emergence had higher correlation with final germination percentage, seedling strong percentage and seedling vigor index. Thus, the standard germination test had high ability for predict percentage and rate of seedling emergence in field
Volume 4, Issue 1 , March 2015, , Pages 27-38
Abstract
In order to investigate the effects of increasing soluble phosphorus (P) on seed production and quality of black seed (Nigellasativa L.) in a calcareous soil, a field experiment was conducted at Faculty of Agriculture, Ferdowsi University of Mashhad, Iran, in 2013. Field experiment was conducted as factorial ...
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In order to investigate the effects of increasing soluble phosphorus (P) on seed production and quality of black seed (Nigellasativa L.) in a calcareous soil, a field experiment was conducted at Faculty of Agriculture, Ferdowsi University of Mashhad, Iran, in 2013. Field experiment was conducted as factorial layout based on a complete randomized block design with four replications. The fertilizer resources (vermicimpost (V) + Tiobacilus (T), Sulfur (S) + T, V+S+T and control) and three levels of P (0, 30 and 60 kg.ha-1) were the first and second experimental factors, respectively. Seed vigor was also assessed by radicle emergence (RE) test for further evaluation of the effects of P on mother plant on the quality of producing seeds. A germination percentage at day 4 was used as seed vigor test. The resources of soil amendment (V+T, S+T and V+S+T) significantly decreased the P concentration in coat, P percentage of coat to seed and mean germination time. For instance, by applying V+S+T, P concentration in coat and mean germination time were decreased by 18 and 10%, as compared to control treatment.However, 1000 seed weight, seed vigor, P concentration in embryo and seed were significantly increased by applying the resources of soil amendment. There was a significant negative relationship between 1000 seed weight and P concentration in coat (R2= 0.66 **). It seems that under P-deficient conditions in soil, mother plant prefer to produce more longevity seeds, instead to increase theseed vigor, by increasing the P allocation to seed coat
Afshin Mozafari; Sajad Fathollahy
Abstract
In order to Investigation the Effect of Seed Biopriming with Plant Growth Promoting Rhizobacteria (PGPR) on antioxidant enzymes activity of seedling and Germination Indices of Two Wheat Cultivar under Salt Stress Conditions, an experiment was conducted as a factorial experiment based in a completely ...
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In order to Investigation the Effect of Seed Biopriming with Plant Growth Promoting Rhizobacteria (PGPR) on antioxidant enzymes activity of seedling and Germination Indices of Two Wheat Cultivar under Salt Stress Conditions, an experiment was conducted as a factorial experiment based in a completely randomized design with three replications in a laboratory of Islamic Azad University branch of Ilam, in 2017. Experimental factors included salt stress at four levels: zero (Control), 50, 100 and 200 mM sodium chloride, PGPR bacteria in two levels: non-inoculation with PGPR (control) and seed inoculation with PGPR and wheat cultivars include: Tajan and Ofogh. The genus and species of PGPR bacteria used in this study included Azotobacter chroococcum, Azospirillum berasilense and Pseudomonas potida, which were obtained from the Iranian soil and water research institute. Experimental traits included Activity of superoxide dismutase enzymes (SOD), catalase (CAT) and glutathione peroxidase (GPX), dry weight of radicl, gomul and seedling, germination percentage, germination rate and seed vigor index. The main effect of experimental factors on all traits was highly significant (P≤0.01). The results showed that Ofogh was superior to Tajan cultivar for all germination indices, dry weight of radicl, gomul and seedling, Activity of antioxidant enzymes. With increasing salt stress, all of traits were decreasing. Priming seeds with PGPR bacteria increased the traits studied. In general, priming of wheat seeds with PGPR in comparison with control treatment (non-inoculation) by improving germination indices and Activity of antioxidant enzymes to some extent reduced the harmful effects of salinity stress.
Maryam Palizdar; Reza Tavakkol Afshari; Ziba Jamzad; Mohammadreza Ardakani; Parisa Nejat Khah
Abstract
This research was carried out to evaluate the germination response of eleven Salvia species to application of Gibberellic acid (GA3) and pre-chilling treatments in the Iran Research Institute of Forests and Rangelands during 2012. Germination responses of the seeds were investigated under control treatment, ...
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This research was carried out to evaluate the germination response of eleven Salvia species to application of Gibberellic acid (GA3) and pre-chilling treatments in the Iran Research Institute of Forests and Rangelands during 2012. Germination responses of the seeds were investigated under control treatment, 2-levels of Gibberellic acid (GA3) treatments with 500 mg/L (for 24 and 48 hours) and 2-levels of pre-chilling treatments at 4°C (for 2 and 4 weeks). The germination percentage and rate, as the physiological traits of the species, were studied based on a completely randomized design with four replications. Based on the results, applying the Gibberellic acid (GA3) treatment for 24 hours increased both the germination percentages and rates as compared to control; but the treatment for 48 hours was insignificantly changed the two mentioned parameters comparing to control. On the other hand, the pre-chilling treatment of seeds for 2 weeks also caused a significant increase in the above parameters as compared to the control treatment; while they were insignificantly different from the control treatment by the use of pre-chilling treatment for 4 weeks.
Volume 1, Issue 1 , March 2012, , Pages 28-37
Abstract
Effects of Aspergillus f lavus on seed germination traits, germination percentage, seed germination rate and abnormality during short storage periods, of Mountain rye )Secale montanum( were evaluated. Seeds of Mountain rye contaminated with 106/ ml spore suspension of this Aspergillus flavus. The control ...
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Effects of Aspergillus f lavus on seed germination traits, germination percentage, seed germination rate and abnormality during short storage periods, of Mountain rye )Secale montanum( were evaluated. Seeds of Mountain rye contaminated with 106/ ml spore suspension of this Aspergillus flavus. The control and contaminated seeds were maintained at 65, 75 and 86% relative humidity, and the storage temperatures of 15, 25 and 35˚C. Related traits were measured at the end of storage period. Spore populations under each condition was determined. Seed germination percentages and rates of contaminated seeds decreased compared to the control seeds. The most abnormality was observed with contaminated seeds in 35˚C after one week. The least spore population was related to seeds stored under 65% relative humidity equivalent to 13% at 15anddeg;C. Significant differences were found for germination percentage between treated and control seeds.
N. Zamani; P. Roshandel
Abstract
Salt tolerance of six halophytic species (Halocnemum strobilaceum, Halostachysbelangeriana, Salsola tomentosa, Salicornia europea, Halopeplis perfoliata,Salsola crassa) was evaluated at germination stage under NaCl conditions (0, 1, 2, 3, 4 and 5%). All seeds showed full germination in 1 and 2% NaCl, ...
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Salt tolerance of six halophytic species (Halocnemum strobilaceum, Halostachysbelangeriana, Salsola tomentosa, Salicornia europea, Halopeplis perfoliata,Salsola crassa) was evaluated at germination stage under NaCl conditions (0, 1, 2, 3, 4 and 5%). All seeds showed full germination in 1 and 2% NaCl, comparable to germination in distilled water. By increasing the levels of NaCl, the differences between these species responses were evaluated. The second three species (from coastal zone of the salty lake Maharloo) were more tolerant compared to the first three species (from Gavkhooni deserts). Amongst, S. europea was the most tolerant species. At the second round of experiments and to test the effect of jasmonic acid (JA) on seed germination at high levels of salinity, the seeds were primed with JA (0, 0.01, 0.1, 1, 10 and 100µM) and then allowed to germinate in Petri-dishes containing different concentrations of NaCl: 3, 4 and 5% for Gavkhooni species and 4 and 5% for Maharloo species. Priming with 1 µM JA was the most effective to increase germination characters at high levels of salinity. By increasing the concentration of JA (from 10 to 100 µM) the inhibitory effects of JA on seed germination of the mentioned species was appeared.
Mohadeseh Shamsaddin Saied
Abstract
Environmental stresses, including drought and salinity stress can play an important role in reducing plant growth and yield especially in the seedling establishment stage in arid and semi-arid climates of Iran.Therefore, identification of range plants tolerance to drought and salt is very important.To ...
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Environmental stresses, including drought and salinity stress can play an important role in reducing plant growth and yield especially in the seedling establishment stage in arid and semi-arid climates of Iran.Therefore, identification of range plants tolerance to drought and salt is very important.To assay the effect of different concentrations of drought and salinity on germination and early growth of camelthorn(Alhagi maurorum) two experiments were performed separately in a completely randomized design with three replications in the laboratory of Bardsir Faculty of Agriculture, Shahid Bahonar University of Kerman.30 uniform seeds were sterilized with 10% sodium hypochlorite solution for 30 seconds and after washing the seeds three times with distilled water, they were placed in 9 cm Petri dishes on Whatman No. 2 filter paper.In the first experiment, polyethylene glycol solution (PEG6000) with concentrations of -1, -1.5 and -2 MPa was applied to obtain the osmotic potential, and in the second experiment, NaCl with concentrations of 100, 150 and 200 mM was used.Distilled water was also applied to make zero stress(control)in both experiments. Results showed that camelthorn seeds were able to germinate at -2 MPa of drought potential and 200 mM salinity potential.However, increasing drought and salinity stress significantly reduced germination percentage and rate, seed vigor index, seedling dry weight, shoot length, root length, shoot dry weight and root dry weight.Assessing the response of this plant to different levels of drought and salinity in the germination stage and early growth of seedling is important for forage production, soil protection and medicinal value
Volume 3, Issue 1 , March 2014, , Pages 31-40
Abstract
Seed priming improves some properties of seedling. The objective of this research was to evaluate the effect of salicylic acid and gibberellins on germination characteristics and changes of biochemically of sunflower seeds under accelerated aging, experimental design was factorial with complete randomized ...
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Seed priming improves some properties of seedling. The objective of this research was to evaluate the effect of salicylic acid and gibberellins on germination characteristics and changes of biochemically of sunflower seeds under accelerated aging, experimental design was factorial with complete randomized design as base design with 3 replications. The first factor was priming by salicylic acid and gibberellin, secondary factor was combinations 4 levels of aging (0, 2, 4 and 6 days of aging at 41°C). The results showed that the priming and aging effects on germination percentage, germination index, normal seedling percentage, seedling length, vigor index, abnormal seedling percentage, catalase and ascorbate peroxidase were significant, but on mean time to germination not significant. Results showed that, the highest germination characteristics were attained from priming by salicylic acid and gibberellins under non aged conditions. Also, seed priming increases enzyme activity.
Abstract
In order to study of acid gibberellic pretreatments on the enhancement of aged seed germination and seedling growth of Tanacetum parthenium in laboratory condition, a factorial experiment based on completely randomized design with three replications was conducted in seed technology laboratory in research ...
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In order to study of acid gibberellic pretreatments on the enhancement of aged seed germination and seedling growth of Tanacetum parthenium in laboratory condition, a factorial experiment based on completely randomized design with three replications was conducted in seed technology laboratory in research institute of forests and rangeland, Tehran, Iran in 2015. The factors A were: conservation in basic cold room (-18◦C), active cold room (+4◦C), regenerated seeds and aged seeds (48 h) and factor B were: osmopriming (PEG 0.3 and 0.6Mpa), hormon priming (500 and 1000 ppm of Acid Gibberlic), hydropriming (distilled water) and control. The usual germination characteristics were measured. Higher germination percentage and speed of germination and seedling length were obtained from regenerated seed which conserved at( 24Cº). The more germination characteristics were observed on the seeds which conserved in active room with duration of 10 to 20 years. The minimum germination characteristics were in aged seeds by artificial conditions. Highest germination characteristics were with Gibberlic Acid ( 1000 ppm) . Maximum some of germination traits were observed with osmopriming technique (Poly ethylene glycol 0.6 Mpa). The highest germination traits were obtained with Gibberlic Acid of (1000 ppm ) and Poly ethylene glycol of (0.6 Mpa), in active cold room. In base cold room seed reservoir, effect of osmopriming ((Poly ethylene glycol 0.6 Mpa) were more than other treatments while in aged seeds of artificial conditions, osmoprimin (Poly ethylene glycol 0.6, 0.3 Mpa) have more effect on aged seeds retrieval compare with other treatments
hamid reza javanmard; hossein mokhtari karchegani
Abstract
In order to investigation the effects of different concentrations of leaf aqueous extracts of walnut on seed germination and seedling growth of barley, arugula and corn, a laboratory experiment were conducted in the Faculty of Agronomy, Islamic Azad University of Isfahan, in 2013. In this experiment, ...
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In order to investigation the effects of different concentrations of leaf aqueous extracts of walnut on seed germination and seedling growth of barley, arugula and corn, a laboratory experiment were conducted in the Faculty of Agronomy, Islamic Azad University of Isfahan, in 2013. In this experiment, walnut leaf extract including control (distilled water), 25, 50, 75 and 100% were studied to determine juglone sensitivity in three species based on completely randomized design (CRD) with four replications. Chromatography analysis showed that a highest rate of juglone was observed on walnut leaves in August. Increasing concentration of leaf extract lead to continuously decrease in germination percentage and germination rate, root and shoot length and seedling dry weight. Germination percentage of corn and arugula seeds on primary growth stage showed resistant to walnut allelopathy. The lowest germination percentage was observed on barley in %100 leaf aqueous extracts of walnut. Corn and arugula were more sensitive to walnut extracts than barley seeds, however, root and shoot dry weight in barley had no significantly different between treatments. The aqueous extract of walnut did not have a significant effect on germination components of barley, corn and arugula, so that corn and arugula in the early stages of germination and barley in seedling growth showed strong resistance. The insensitivity of the studied species showed that the aqueous extract of Iranian walnut leaves is a strong substance in controlling weeds of these species towards sustainable agriculture.
sepideh nikoumaram; Reza Tavakkol Afshari
Abstract
An experiment was done in the research laboratory of Tehran university in order to evaluate the effect of various conditions of seed storage on storability of rapeseed primed seed. The experiment factors have included the seed priming using .05% lotion of KNO3 for 12 hours (primed and non primed with ...
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An experiment was done in the research laboratory of Tehran university in order to evaluate the effect of various conditions of seed storage on storability of rapeseed primed seed. The experiment factors have included the seed priming using .05% lotion of KNO3 for 12 hours (primed and non primed with a this lotion)–package (vaccum packaging, nano and aluminium bags)–seed storage condition including: moisture content (5, 9, 13 and 17 %), temperatures(15 and 30) and storage periods(1,2,3,4,5 and 6 months). Analysis of variance demonstrated that with increasing the duration of storage and also with increasing seed content humidity amount and storage temperature, the seeds vigour would be decreased and in studing indexes, this decrease occurred in the first month of storage in 17% seed water content and 30 C temperature treatment and not in other onces, which shows that storage condition in more important in storage than other factors. For all indices, germination rate and percentage in rapeseed seed, there were significant differences in main, double and triple interaction effects at 1% probability level. the nano packagings is usually suggested as a suitable packaging to preserve the seeds in these condition.
Mahboobe Mohammadi; Mohammad Hassan Rashed Mohassel; Ehsan Neamatollahi
Abstract
This experiment was designed to investigate the effect of seed coating with micronutrient elements, regulators and growth stimulants on the characteristics of germination and seedling establishment of sugar beet seeds, and in the form of a completely randomized design with four repetitions in the year ...
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This experiment was designed to investigate the effect of seed coating with micronutrient elements, regulators and growth stimulants on the characteristics of germination and seedling establishment of sugar beet seeds, and in the form of a completely randomized design with four repetitions in the year 2020, in the Razavi Seed and Plant Institute was carried out under laboratory and greenhouse conditions. The treatments of this experiment were different combinations of micronutrient elements, regulators and growth stimulants which included 29 treatments along with a control treatment (without coating). The results showed that germination percentage, daily germination rate, germination rate coefficient, root and shoot length, root and shoot dry weight, and seedling root length index were significantly affected. Seed coating treatments were applied. In general, treatment 21, including macro elements + micro elements + humic acid + Gibberellic acid, with a germination percentage of 97%, seedling length index 13.87, allometric coefficient 0.2232, as the best treatment, increasing the mean You have a witness about the treatment. Also, among the different seed coating treatments, treatment 22, including microelements + humic acid + Gibberellic acid + kaolin, had the highest percentage of seedling establishment with 95%. Seed coating with micronutrients, humic acid and Gibberellic acid had the greatest effect on improving seedling growth and quality and germination percentage.
Nasrin Teimoori; Mohsen Saeidi; Mahmood Khoramivafa; Shahab Khoshkhoi
Abstract
The utilization of nanoparticles for seed priming may enhance the germination and seedling growth compared to bulk forms. Therefore, this research aims to investigate the effect of seed priming (with bulk iron oxide, nanoparticles of 1-100 nm and 40-60 nm in concentrations of zero, 2, 4, and 8 g l-1, ...
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The utilization of nanoparticles for seed priming may enhance the germination and seedling growth compared to bulk forms. Therefore, this research aims to investigate the effect of seed priming (with bulk iron oxide, nanoparticles of 1-100 nm and 40-60 nm in concentrations of zero, 2, 4, and 8 g l-1, water, and no priming) at different levels of drought stress (including no stress, -2, -4 and -8 bar) using polyethylene glycol 6000 on the characteristics of germination and growth of Adel chickpea cultivar. This research was performed as a factorial experiment based on a completely randomized design in three replications at Razi University. The results showed that germination ceased completely under drought stress of -8 bar. Drought stress at -4 bar after -8 bar resulted in a significant decrease in the percentage, speed, and vigor of germination by 96, 93, and 40%, respectively, and 130% increase in root length/shoot length ratio compared to the non-drought stress treatment. Seed priming treatments had the most positive and significant effect on the speed and vigor of germination in non-drought stress treatment and on the germination percentage in drought stress treatment. The seed priming with iron 8 g l-1 and 40-60 nm produced the highest length and weight vigor of germination. It appears that the priming treatments have resulted in better vigor and rate of germination in non-drought stress condition while germination percentage in drought stress. The seed priming treatment using iron 40-60 nm is the most effective compared to other priming treatments.
Masoud Ahmadzadeh; Zahra Saber Baghban; Hamid reza Haddadi
Abstract
Nanotechnology is a powerful new technology, that can create a huge revolution in food supply and agriculture in global scope. In This research the effects of different concentrations of silver nano particles was studied on germination factors of two cultivars of cotton (Sayokra & Varamin) and one ...
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Nanotechnology is a powerful new technology, that can create a huge revolution in food supply and agriculture in global scope. In This research the effects of different concentrations of silver nano particles was studied on germination factors of two cultivars of cotton (Sayokra & Varamin) and one cultivar of mays (Single Cross 704) and the effects of these concentrations on MIC, MBC in (Xanthomonas smithii) as a seed-born bacteria and the agent of blight disease on cotton. The treatments used at this experiment including 20, 40, 60, 80, 100, 120, 140, 160, 320, 640 µl/l concentrations of silver nano particles. Their effect were studied on seed germination rate, root, shoot and seedling length. maximum amount of shoot and seedling length in Sayokra was ralated to 120 µl/l and in Varamin cultivar was related to 60 µl/l. About mays cultivar, root, shoot and seedling length had highest and lowest amount at 80 and 160 µl/l respectively, and about the germination rate the lowest amount was relevant to 640 µl/l. in this study the MIC of (X. smithii) was 0.5 µl/l and the 100% inhibitor concentration was 15 µl/l.
Azadeh kavandi; Amir Ghorbankhani; Fereshta shahbazi Asl
Abstract
Seed deterioration is one of the factors reducing seed viability and vigor. In order to investigate the effect of osmopriming on seed vigor enhancement and seedling enzyme activities, a factorial experiment was conducted in a completely randomized design with four replications in greenhouse of Research ...
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Seed deterioration is one of the factors reducing seed viability and vigor. In order to investigate the effect of osmopriming on seed vigor enhancement and seedling enzyme activities, a factorial experiment was conducted in a completely randomized design with four replications in greenhouse of Research Institute of Forests and Rangelands, Tehran, Iran in 2015. Experimental factors were four sainfoin species as (Onobrichis cristagalli, O. micoxchi, O. sintensii and O. vicifolia), three levels of deteriorated seeds using accelerated ageing techniques (40°C, 98% of RH for 48 and 72h and control) and three priming treatments including hydropriming (control) and osmopriming (PEG -0.6Mpa and -1.2 Mpa). Data were collected for germination percent, rate of germination, root length, shoot length, vigor index, seedling weight and the variability in the activities of three antioxidant enzymes as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD). Results showed that seed deterioration decreased germination traits in all of sainfoin species. Priming by seed aging interaction effect was significant for most of traits. The O.vicifolia had higher mean values for germination traits in deteriorated seeds. The result indicated that application of PEG (-0.6Mpa and -1.2 Mpa) had improved seedling growth as root and shoot length in all of the species. Measurement of enzymatic activity showed that the activities of antioxidant enzymes were decreasing in aged seeds of sainfoin. However, application of PEG (-0.6Mpa) had improved enzymatic activity in aged seeds. It was concluded that osmopriming may enhance seed vigor, seedling growth and enzyme activities in deteriorated seed of sainfoin
A. Hashemi; R. Tavakkol Afshari; L. Tabrizi; Sh. Barooti
Abstract
In many studies, the medicinal properties and impact of plantago ovata on mechanisms of human physical activities has been proven. So that one of the most useful, effective and harmless herbal laxative. drought is one of the limiting factors and a serious danger for the successful production of crops. ...
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In many studies, the medicinal properties and impact of plantago ovata on mechanisms of human physical activities has been proven. So that one of the most useful, effective and harmless herbal laxative. drought is one of the limiting factors and a serious danger for the successful production of crops. An experiment was conducted to study germination characteristics of plantago ovata under water and drought stress conditions at different temperatures. The experiment was conducted at the University of Tehran lab, Karaj, in 2015. Temperatures regimes included 5, 10, 15, 20, 25, 30, and 35 °C). For each temperature, the treatment for drought and water stress was four potentials (zero, -4, -8 and -12 bar) with sodium chloride. The effects of drouth stress on seed germination of plantago ovata at different temperatures showed the highest resistance to drought at 20 º C. drouth stress at different temperatures reduced germination parameters nad in the potential -12 germination stopped. Increased temperatures resulted in better conditions for seed germination and increase the drought stress tolerance. Increased severity of drought causes the reduction of temperature range in which the seed can germinate.
Forough Firouzkoohi; Fatemeh Nasibi; Batool Keramat; Hadi Noori; Effat Ahmadi Mousavi
Abstract
Effects of dielectric barrier discharge (DBD) Helium plasma treatments on guar (Cyamopsis tetragonoloba) seed germination and seedling growth were studied. Seeds were pretreated with 0, 1/44 and 0/68 W of cold plasma for 60 s. Salt stress experiment included three salinity levels of NaCl (0, 50 and 100 ...
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Effects of dielectric barrier discharge (DBD) Helium plasma treatments on guar (Cyamopsis tetragonoloba) seed germination and seedling growth were studied. Seeds were pretreated with 0, 1/44 and 0/68 W of cold plasma for 60 s. Salt stress experiment included three salinity levels of NaCl (0, 50 and 100 mM). Results showed that plasma treatments had positive effects on seed germination and seedling (shoot length, root length, dry and fresh weight of shoot and root) , The treatment of 1.44 W of cold plasma had the most stimulating effect on the percentage of germination and growth of guar seedlings. Additionally, the application of Helium plasma treatments, considerably enhanced chlorophyll a, chlorophyll b, total chlorophyll, carotenoids, and potassium(k) content in the leaves of guar plant. Thus, cold plasma treatment can be used an ameliorative way to the enhancement germination and improve seedling growth of guar against damage caused by salt stress. Key words: dielectric barrier discharge, seed germination, guar, cold plasma, salt stress
Samira Moghadam; Saeideh Maleki Farahani; Samar Khayamim
Abstract
To evaluate the effect of salinity stress on seed germination and screening of sugar beet genotypes, 20 polygerm and 15 sugar beet drought tolerant genotypes were separately tested in between paper and rapid germination test in Erlen under control and 20 dSm-1. Also they were tested in rapid germination ...
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To evaluate the effect of salinity stress on seed germination and screening of sugar beet genotypes, 20 polygerm and 15 sugar beet drought tolerant genotypes were separately tested in between paper and rapid germination test in Erlen under control and 20 dSm-1. Also they were tested in rapid germination test in Erlen and green house experiment under control and 16 dSm-1 in factorial experiments based on completely randomized designs with four replicates. Salinity stress in rapid germination test decreased germination index over drought tolerant and polygerm sugar beet genotypes about 80 and 90%, respectively in comparison to normal. Rapid germination test in Erlen was found to be better and more suitable in comparison of sugar beet genotypes under salinity in comparison to between paper tests. Genotypes which had more field emergence potential in laboratory had more total dry weights in green house under salinity condition. There was positive and significant correlation between fast germination tests in EC=20 and 16 dSm-1, so it seems that the rapid germination method in Erlen in EC=16 dSm-1 is more efficient to evaluate sugar beet genotypes under saline conditions
zhila nazari; Mohammad Sedghei; Hamed Narimani
Abstract
In order to investigate the effect of humic acid, iron and silicon nanoparticles on quantitative and qualitative yield and filling components of wheat seed under salinity conditions, a factorial layout was carried out based on a randomized complete block design with three replications in the research ...
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In order to investigate the effect of humic acid, iron and silicon nanoparticles on quantitative and qualitative yield and filling components of wheat seed under salinity conditions, a factorial layout was carried out based on a randomized complete block design with three replications in the research greenhouse of University of Mohaghegh Ardabili during 2021. Treatments included salinity levels (without salinity as a control, salinity of 35 and 70 mM by NaCl and without salinity as a control), foliar application of nanoparticles at four levels (no foliar application as a control, foliar application of 0.8 per liter of nano iron, 40 mg / l nanosilicon spraying, combined application of iron nanoparticles and silicon nanoparticles and no foliar application as a control) and humic acid spraying (spraying with water as a control and spraying 3 g/l. The results showed that combined application of humic acid and foliar application of 40 mg of iron and nanosilicon nanoparticles in the absence of salinity, increased maximum seed weight, seed filling rate, length and effective seed filling period, Spike length, 100-seed weight, plant height, number of seeds per spike, chlorophyll index (56.60, 1.183, 39, 58.45, 58.5, 39.53, 2.51, 70.6, and 57.57 respectively) and increased the yield of single plant (46.97%) compared to the conditions of non-application of humic acid and nano-oxides under the salinity of 70 mm of soil. Nanooxides (iron and silicon) can increase wheat seed yield of wheat under salinity stress conditions by improving seed filling components.
Tayebeh Alsadat Cheraghi Takht Choobi; Seyed Amir Moosavi; Ahmad zare; Ahmad KoochekZade; ghasem parmoon
Abstract
In order to determine the cardinal temperature of decayed sugarcane seeds, this study was carried out factorially in a completely randomized design with three replications in Khuzestan University of Agriculture and Natural Resources. The first factor was aging at 5 levels (0, 24, 48, 72 and 96 hours ...
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In order to determine the cardinal temperature of decayed sugarcane seeds, this study was carried out factorially in a completely randomized design with three replications in Khuzestan University of Agriculture and Natural Resources. The first factor was aging at 5 levels (0, 24, 48, 72 and 96 hours of decline) and germination temperatures at 7 levels (5, 10, 15, 20, 25, 30, 35 and 40 ° C). The results showed that the percentage and speed of germination were affected by the interaction of temperature in the decay so that their highest value was in the temperature range of 20 to 25 ° C and the decay in addition to reducing their amount increased the range of changes of this plant at different temperatures. The highest germination percentage was observed at 20 ° C in undeveloped seeds and 15 to 20 ° C at 24 and 48 h. Estimation of different non-linear models on germination rate also showed that among the evaluated models, the better toothed model was the model for germination of sugarcane seeds at different levels of decay. The germination basal temperature of sugarcane was estimated to be 1 ° C in non-degradable conditions, which increased to 1.8 ° C in 24 hours of decline and decreased again in higher degradations. The optimum germination temperature of this plant was between 20 to 32 at no aging conditions.
Nesa Gharehbaghli
Abstract
With the aim of evaluating the effect of salicylic acid in improving the efficiency of the aged pumpkin seeds under drought stress conditions, a factorial experiment was carried out in the form of a completely randomized design with three replications. Pretreatment of different concentrations of salicylic ...
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With the aim of evaluating the effect of salicylic acid in improving the efficiency of the aged pumpkin seeds under drought stress conditions, a factorial experiment was carried out in the form of a completely randomized design with three replications. Pretreatment of different concentrations of salicylic acid including zero (pretreated with water), 1 and 1.5 mM at different drought levels of zero, -0.2, -0.4 and -0.6 MPa were investigated. Indicators of mean germination time, germination percentage, seedling length, seed vigor, membrane electrolyte leakage, soluble carbohydrates, soluble proteins, malondialdehyde, ascorbate peroxidase activity, superoxide dismutase and catalase enzymes were evaluated. The results showed that the pretreatment of seeds with different concentrations of salicylic acid prevented the significant decrease in the germination indices of the deteriorated pumpkin seeds under drought stress. So that at the potential of -0.6 MPa, the treatment of seeds with 1.5 mM salicylic acid increased the percentage of germination, seed germination index, soluble carbohydrates and soluble proteins respectively 80.2, 169.4, 51.6, 93.4% and for the activity of ascorbate peroxidase, superoxide dismutase and catalase enzymes, 41.2%, 12% and 11.8%, respectively, compared to the control. Based on the research findings, pretreatment of salicylic acid (1.5 mM) is recommended in order to reduce the oxidative stress caused by drought for pumpkin.
Sara Yavari Ramsheh; Fatemeh Dehghan Nayeri
Abstract
In this research, morphological and biochemical traits of 10 isolates of Pseudomonas fluorescens bacteria, salt tolerance and the effect of bacteria on germination and seedling growth indicators of sesame cultivars under salt stress were conducted. All isolates were Gram-negative and had positive motility ...
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In this research, morphological and biochemical traits of 10 isolates of Pseudomonas fluorescens bacteria, salt tolerance and the effect of bacteria on germination and seedling growth indicators of sesame cultivars under salt stress were conducted. All isolates were Gram-negative and had positive motility and fluorescent properties. Among the bacteria, P2, P3 and P9 isolates were more capable of solubilizing inorganic phosphorus. The ability to produce siderophore was higher in some isolates and the isolates P1, P2, P3, P8, and P10 showed the highest in vitro salt-tolerance. A factorial experiment including two sesame cultivars, 4 salinity levels and inoculation with 3 isolates was done in the form of a completely randomized design in three replications. Based on the results, the effect of bacteria, salinity, variety and their interaction on the percentage and rate of germination, germination indices, allometric coefficient and seedling length and weight index were significant. Under salt stress, the germination and growth of seedlings significantly decreased but, the pretreatment of sesame seeds with salt-tolerant isolates increased the characteristics related to germination and growth indices of sesame cultivars. The highest effect on all parameters including germination rate belonged to P9. Therefore, P9 isolate can be used to increase the tolerance of sesame to salinity stress.
Abolghasem Hamidi Moghaddam
Abstract
Henna (Lawsonia inermis L.) is an ornamental and medicinal plant containing Lawson’s active substance, which is cultivated in some regions of the south and southeast of Iran. Salinity is one of the major abiotic environmental stress that affects almost all stages of plant development. Polyamines ...
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Henna (Lawsonia inermis L.) is an ornamental and medicinal plant containing Lawson’s active substance, which is cultivated in some regions of the south and southeast of Iran. Salinity is one of the major abiotic environmental stress that affects almost all stages of plant development. Polyamines such as putrescine can play important roles in plant growth and stress responses. In order to investigate the effect of putrescine and NaCl salinity stress on some germination indices, a factorial experiment was conducted with two levels of putrescine (0 and 0.75 mM) and four salinity levels (0, 50, 100 and 150 mM) based on completely randomized design with four replications in germinator for 11 days. The results showed that at control salinity level, there was no significant difference between putrescine treatments on Henna seed germination indices except the seed vigor index. The interaction between putrescine and salinity stress showed that pre-treatment with putrescine alleviated the negative effects of 50 and 100 mM salinity stress on the germination rate. However, it increased the negative effects of 150 mM salinity stress on germination percentage, radicle length, plumule length, seedling fresh weight, seed vigor index and α-amylase activity in comparison to control. The results showed that germination of Henna seeds is sensitive to salinity stress and they are only able to tolerate low levels of salinity (50 mM). According to a significant correlation between α-amylase activity with germination percentage (r= 0/98), it can be concluded that probably the change in α-amylase activity decreased the germination percentage.
