Maryam Zamanian; Reza Tavakkol afshari; Jafar Nabati; Alireza Seifi
Abstract
This study was conducted as a factorial experiments based on a completely randomized design with two factors cultivars (Modena and Okapi), aging level (0, 24 and 72 hours at temperature of 40˚c and relative humidity of saturated) and four replications to evaluate germination rate and percentage (GP), ...
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This study was conducted as a factorial experiments based on a completely randomized design with two factors cultivars (Modena and Okapi), aging level (0, 24 and 72 hours at temperature of 40˚c and relative humidity of saturated) and four replications to evaluate germination rate and percentage (GP), vigor index (SV), electrical conductivity (EC), catalase (CAT), ascorbate peroxides (APX), glutathione reductase (GR) and S-nitrosothiol (SNO) at the Ferdowsi University of Mashhad, 2019-20. Accelerated Aging (AA) treatments made significant decline in germination indices and biochemical characteristics (except EC) of canola seeds. Also, Modena cultivar was stronger in germination traits while Okapi cultivar was better in biochemical activities, which can be explained by difference in the primary genetic of the cultivars. According to the results, in the treatments of 72 hours of AA compared to the control, GP has decreased by 86%, CAT 88%, APX 89% and SNOs 36%. On the top of that, there was a positive correlation between SNOs and enzymes. In conclusion, Nitric oxide (NO) is likely to indirectly affect the antioxidant enzymes by SNOs in aged canola seeds. Seed aging targets the cell physiology by reducing the activities of antioxidant enzymes and NO and therefore increasing the rate of cell mortality.
Miad Haji Mahmoodi; Abdolmahdi Bakhshande; Seyed Amir Moosavi; seyed ata Siadat
Abstract
Aging and salinity stress are main challenges towards of producing a healthy seedling. This research was conducted to investigate the effects of seed aging on the seed germination properties of Wild mustard under salinity stress condition. Experimental treatments were five levels of seed aging 0, 24, ...
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Aging and salinity stress are main challenges towards of producing a healthy seedling. This research was conducted to investigate the effects of seed aging on the seed germination properties of Wild mustard under salinity stress condition. Experimental treatments were five levels of seed aging 0, 24, 48, 72 and 96 h and different levels of salinity stress 0, 100, 200, 300, 400 and 500 mmol. Seed germination, germination rate and seed vigor were declined at high salinity concentrations. Catalase activity and total seed protein content were decrease due to the aging. Seed protein content (1.4 mg/g fw) declined about 92% and reach to 0.1 mg/g fw after 96h of aging. Catalase activity was decrease due to aging while in contrast, peroxidase activity was higher from 0.04 to 0.11 u/ mg protein after 96 h of aging. It was observed that all fatty acids of wild mustard seed oil were changed at higher aging durations. Behenic acid (C22:0) and Erusic acid (C22:1) were 0.656 and 38.543% in noaged seeds and they increased to 0.751 and 40.186%, respectively.
