Mahboubeh Hajiabbasi; Reza Tavakkol afshari; A.R. Abbasi; Reza Kamaei
Abstract
Glycine max is an important oilseed crop in all of the world. One of the problems of the G. max production is seed deterioration during seed storage, resulting in low germination and vigor before planting. In order to investigate the effects of ethylene and salicylic acid on the improvement of deteriorated ...
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Glycine max is an important oilseed crop in all of the world. One of the problems of the G. max production is seed deterioration during seed storage, resulting in low germination and vigor before planting. In order to investigate the effects of ethylene and salicylic acid on the improvement of deteriorated soybean seed, accelerated aging tests were conducted for 0, 6 and 10 days and natural aging test for 6 months. After the aging conditions, seeds were imbibed by 50 mM salicylic acid and 10 mM ACC (precursor of ethylene) for 6 hours at 25 °C. Also, a batch of the seed was used after the natural and accelerated aging tests without any hormonal treatment as a control (called dry seeds). Germination percentage, electrical conductivity and gene expression of AMY1, BMY1 were investigated in the dry seed, imbibition of water, salicylic acid and ACC at 6, 12 hours by the qRT-PCR method. The seed germination decreased and electrical conductivity increased with the progress of the aging. Gene expression varied in different days and hours. Salicylic acid and ACC had different effects on measured traits. The highest germination rate was observed in non-aged dry seeds (95%). On the other hand, ACC and salicylic acid failed to change seed germination of 10-day aged seeds. Moreover, the highest electrical conductivity (53 µScm-1gr-1) was in 10-day aged seeds affected by SA. The expression of AMY1 and BMY1 genes increased coincide with the age levels. Salicylic acid reduced the expression of AMY1 in non-aged seed, implicated that salicylic acid works as a stress factor in non-aged seeds. The expression of AMY1 gene in the non-aged seeds was 1.8, while it was 3.3 in 10-aged seed. Overall, aging caused disturbing of seed physiology and salicylic acid and ACC were not able to improve germination of deteriorated soybean seed
