Leyla Cheheltanan; Ali Tehranifar; Mahmoud Shoor; Seyyed Hossein Neamati; Saeed Khosravi
Abstract
Self-pollination induces a phenomenon known as inbreeding depression in heterozygous plants, which in turn leads to a reduction in seed germination and seedling growth. On the other hand, seed priming with plant hormones such as gibberellic acid is a technical approach that potentially results in the ...
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Self-pollination induces a phenomenon known as inbreeding depression in heterozygous plants, which in turn leads to a reduction in seed germination and seedling growth. On the other hand, seed priming with plant hormones such as gibberellic acid is a technical approach that potentially results in the improves of rapid and continuous seed germination and subsequent plant growth. Therefore, this research aimed to investigate the effect of different concentrations of gibberellic acid on the improvement of seed germination resulting from self-pollination in various varieties of Petunia hybrida in a factorial experiment with three replications as a completely randomized design. The treatments consisted of four levels of gibberellic acid (0, 50, 100, and 150 mg/L) and seeds obtained from self-pollination of Iranian Petunia, Tango Blue, Tritunia Pink Morn, and Tritunia White. The results indicated that seed priming with gibberellic acid, especially at a concentration of 100 mg/L, led to a significant increase in the activity of α-amylase and β-amylase by 6.56% and 1.57%, respectively. This increase resulted in 8.54% rise in soluble sugars content, providing energy that significantly enhanced germination percentage, germination speed, average time required for germination, seed vigor index, germination energy, and the fresh and dry weight of plumule and radicle. Moreover, among different varieties, seeds obtained from self-pollination of Iranian petunia demonstrated a higher germination percentage and germination speed compared to seeds from other varieties, indicating a superior capability in maintaining vigor potential, germination percentage, and germination speed.
Sajad Alipour; Ali Tehranifar; Mahmoud Shoor; Leila Samiei; Homayoun Farahmand
Abstract
Fritillaria raddeana Regel is one of the Iranian-native bulbous plants well known for its medicinal and ornamental values. As regards germination and establishment are two key points in order to protect the natural populations of this species, it is important to study about propagation and domestication ...
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Fritillaria raddeana Regel is one of the Iranian-native bulbous plants well known for its medicinal and ornamental values. As regards germination and establishment are two key points in order to protect the natural populations of this species, it is important to study about propagation and domestication of F.raddeana. An experiment was conducted to study the status of seed dormancy to break it in a randomized completely design including 5 priming treatment with 7 replicate in 2015- 2016. Priming treatments were arginine; in concentrations of 5 and 10 mM), gibberellic acid; concentration of 250 and 500 mg per liter, and control (without priming). All events throughout germination process seed was recorded graphically for an image. The results showed that seed priming with arginine and gibberellic acid improved germination properties. Among all treatments, arginine 5 mM has greatest impact on the rate and percentage of germination as far as it increased percentage and rate germination by 1.5 and 2.5 fold in companion with control, respectively. However, there is no significant different between arginine 10 mM and two concentration of gibberellic acid in germination results. The study on the growth of the embryo showed that F.raddeana seeds have after-ripening, so that embryo size is third of its actual size in natural habitat that gradually developed with receiving cold treatment.
Fatemeh Nematollahi; Ali Tehranifar; Seyyed Hossein Nemati; fatemeh kazemi; Gholamali Gazanchian
Abstract
Due to high expenses of imported lawn seeds and low adaptability of them to climatic conditions of Iran, paying attention to native ecotypes of turf grasses is important. One of the reasons for paying less attention to the seeds of native turf grasses is their lack of rapid and uniform germination rate ...
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Due to high expenses of imported lawn seeds and low adaptability of them to climatic conditions of Iran, paying attention to native ecotypes of turf grasses is important. One of the reasons for paying less attention to the seeds of native turf grasses is their lack of rapid and uniform germination rate especially under drought stress conditions. The aim of this study was to investigate the effect of seed priming using salicylic acid (1/0, 5/0, 1 and 5/1 mM), sodium chloride (15, 30 and 45ds /m) and distilled water compared to the control treatment on tall fescue native to GazBorkhar (Isfahan province) under mild, moderate and severe drought stress conditions. For this purpose, seed emergence, mean emergence time, shoot height, plant biomass, relative water content, electrolyte leakage, total chlorophyll content and carotenoid content were measured. The results showed seed priming using salicylic acid 0.5 mM significantly increased the seed emergence rate (96.10%) in moderate stress conditions and reduced the time to emerge (3.70 day) in mild stress conditions. Although the plants treated with 0.5 mM salicylic acid did not show significant differences with the control treatment in other characteristics, considering the significance of the amounts and rates of seed emergence in native turf grasses, this prime treatment can be recommended to improve emergence percentage of the seeds of Festuca arundinacea ecotype Gazborkhar.
