Document Type : Original Article

Authors

1 Department of Horticultural Science and landscape, Ferdowsi University of Mashhad, Iran

2 Department of Horticultural Science and landscape, Ferdowsi University of Mashhad, Iran.

3 . Department of Ornamental Plants, Research Center for Plant Sciences, Ferdowsi University Mashhad. Mashhad, Islamic Republic of Iran.

4 Department of Horticultural Science, Faculty of Agriculture, Shahid Bahonar University of Kerman. Kerman, Islamic Republic of Iran

Abstract

Fritillaria raddeana Regel is one of the Iranian-native bulbous plants well known for its medicinal and ornamental values. As regards germination and establishment are two key points in order to protect the natural populations of this species, it is important to study about propagation and domestication of F.raddeana. An experiment was conducted to study the status of seed dormancy to break it in a randomized completely design including 5 priming treatment with 7 replicate in 2015- 2016. Priming treatments were arginine; in concentrations of 5 and 10 mM), gibberellic acid; concentration of 250 and 500 mg per liter, and control (without priming). All events throughout germination process seed was recorded graphically for an image. The results showed that seed priming with arginine and gibberellic acid improved germination properties. Among all treatments, arginine 5 mM has greatest impact on the rate and percentage of germination as far as it increased percentage and rate germination by 1.5 and 2.5 fold in companion with control, respectively. However, there is no significant different between arginine 10 mM and two concentration of gibberellic acid in germination results. The study on the growth of the embryo showed that F.raddeana seeds have after-ripening, so that embryo size is third of its actual size in natural habitat that gradually developed with receiving cold treatment.

Keywords

Agha Baabazadeh, Z. 2015. Optimization of treatments for improving efficiency, germination and seedling establishment of Fritillaria imperialis (In Persian). Msc.Thesis. Univ. of Faculty of Agriculture and Natural Resources, Shahrekord, Iran. (In Persian, with English Abstract)
Agha Baqbanjad, Z., A.S. Abbas Soraki, and P. Tahmasebi. 2015. Effect of gibberellic acid on seed germination of Fritillaria imperialis (In Persian). The 13th Iranian Conference on Plant Breeding and the 3rd Iranian Seminar on Science and Technology. Karaj.Iran.
Alhadi, F., A. S. Al-Asbahi Adnan, S. A. Alhammadi Arif, and A. A. Abdullah Qais. 2012. The effects of free amino acids profiles on seeds germination/dormancy and seedlings development of two genetically different cultivars of Yemeni Pomegranates. Stress Physiol. Biochem. 8: 1-7.
Arif, M., M.T. Jan, K.B. Marwat, and M.A. Khan. 2008. Seed priming improves emergence and yield of soybean. Pakistan J. Bot. 40: 1169-1177.
Baker, A. 2005. An endangered alpine species of Central Himalaya, India. Plant Biosystems. 148: 1075-1084.
Baskin, C. C. and J. M. Baskin.  (ed.). 2014. Seeds: ecology, biogeography, and, evolution of dormancy and germination. Elsevier.
Canton, F. R., M. F. Suarez and F. M. Canovas. 2005. Molecular aspect of nitrogen mobilization and recycling in trees. Photosyn. Res. 83: 264-278.

Chen, H., B. C. Mc Caig, M. Melotto, H. Yang, S. He and G. A. Howo. 2004. Regulation of plant arginase by wounding, jasmonate and the phytotoxin coronation.  Biol. Chem. 279: 45998-46007.

Dashti, F., H. Ghahremani-Majd, and M. Esna-Ashari (In Persian). 2012. Overcoming seed dormancy of mooseer (Allium hirtifolium) through cold stratification, gibberellic acid, and acid scarification. Forestry. 23:707-710.
De Hertogh, A. and M. Le Nard (Ed). 1993. The Physiology of Flower Bulbs. Elsevier Science Publishing. The Netherlands.
Eslamzadeh, N., S. M. Hosseini, H. R. Moradi and F. Azeri Dehkordi. 2009. Introduction of new habitats for Fritillaria imperialis with using GIS. . (In Persian, with English Abstract.)  Environ. Sci. Technol. 1: 251-261.
Farahmand, H and F. Nazari .2015. Environmental and Anthropogenic Pressures on Geophytes of Iran and the Possible Protection Strategies: A Review. Hortic. Sci. Technol. 2: 111-132.
Gonzalez-Benito, M. E., M. J. Albert, J. M.Irionda, F. Varela and F. Perez- Garca, 2004. Seed germination of four thyme species after conservation at low temperatures at several moisture contents. Seed Sci. Technol. 32: 247-254.
Hamidi, A., D. Roodi, V. Asgari, and S. Hajilooi. 2009. Study on applicability of controlled deterioration vigour test for evaluation of seed vigour and field performance of three oil-seed rape (Brassica napus L.) cultivars. Seed Plant. 24: 677-705. (In Persian, with English Abstract)
Hill, L. 2019. Fritillaria Icones. [Online]. Available at wwwwww.fritillariaicones.com.
Hwang, H.  J., E. H. Kim and Y. D. Cho. 2001. Isolation and properties of arginase from a shade plant, ginseng roots. Phytochem. 58: 1015–1024.
Kaur-Sawhney R., A. F. Tiburcio, and A.W. Galston. 2003. Polyamines inplants: An overview. Cell and Mol. Biol. 2: 1-12.
Khan, A.A. 1992. Preplant physiological seed conditioning. Hortic. Rev. 13:131-181.
King, J. E., and D. J. Gifford. 1997. Amino acid utilization in seeds of loblolly pine during germination and early seedling growth (I. arginine and arginase activity). Plant Physiol. 113: 1125–1135.
Kucera, B., M.A. Cohn and G. Leubner-Metzger. 2005. Plant hormone interactions during seed dormancy release and germination. Seed Sci. Res. 15:281-307.
Nasibi, F., K.M. Kalantari, and A. Barand. 2014. Effect of seed pre-treatment with L-arginine on improvement of seedling growth and alleviation of oxidative damage in canola plants subjected to salt stress. Iran J. Plant Physiol. 5: 1217-1224.
Nasiri, M., P. Babakhanloo, and H. Maddah-Arefi. 2003. First report on braking dormancy and seed germination on Diplotaenia damavandica Mozaffarian. Iranian Rangelands and Forests Plant Breed. and Genet. Res. 2: 258-274.
Nejadalimoradi, H. A., F.A. Nasibi, K. M. Kalantari and R. O Zanganeh. 2014. Effect of seed priming with L-arginine and sodium nitroprusside on some physiological parameters and antioxidant enzymes of sunflower plants exposed to salt stress. Agric Commun. 2014: 23-30.
Nicolas, C., G. Nicolas, and D. Rodriguez. 1996. Antagonistic effects on abscisic acid and gibberellic acid on the breaking of dormancy of Fagus sylvatica seeds. Physiol. Plant. 1996:  244-250.
Panwar, P., and S. D. Bhardwaj. 2005. Handbook of practical forestry. Agrobios (INDIA).
Payal, K., R. K. Maikhuri, K. S. Rao and L. S. Kandari. 2014. Effect of gibberellic acid- and water-based pre-soaking treatments under different temperatures and photoperiods on the seed germination of Allium stracheyi Baker: An endangered alpine species of Central Himalaya, India. Societa Botanica Italiana. 6: 148-155.
Ranal M.A. and D. G. Santana. 2006. How and why to measure the germination process? Revista Brasileir de Botanica. 29: 1-11.
Rouhi, H. R., K. Shakarami, R. Tavakkol Afshari. 2010. Seed treatments to overcome dormancy of waterlily tulip (Tulipa kaufmanniana Regel.). Australian Crop Sci. 4: 718-721.
Tehrani Sharifi, M., M. Advay and L. Shabani .2015. Fritillaria (Liliaceae) in Iran: distribution and Nomenclature. (In Persian, with English Abstract.)  Taxon and Biosy. 22: 70-49.
Tipirdamaz, R and A. N. Gömürgen. 2000. The effects of temperature and gibberellic acid on germination of Eranthis hyemalis (L.) Salisb. Seeds. Turk Jour. Botany.  24:, 143-146.
Todd, C. D., and D. J. Gifford. 2003. Loblolly pine arginase responds to arginine in vitro. Planta. 21: 610-615.
Yagi, M. I., and S. S. Abdulkareem. 2006.  Effects of exogenous arginine and uric acid on eruca sativa mill grown under saline conditions, J. Sci. Technol.7: 1-11.