Hassan Akhgari; Behzad Kaviani
Abstract
Priming has an important role on vigority of seed and plantlet. The first factor was two rice cultivars (Khazar and Hashemi) and the second one was ten priming treatment levels (T1-T10). Examination of seed vigority and seedling assessment was carried out according to the ISTA protocol. Results showed ...
Read More
Priming has an important role on vigority of seed and plantlet. The first factor was two rice cultivars (Khazar and Hashemi) and the second one was ten priming treatment levels (T1-T10). Examination of seed vigority and seedling assessment was carried out according to the ISTA protocol. Results showed that the root fresh weight (14.7 mg) and root number (5) was higher in Khazar than those of Hashemi. The required time (hour) for reach to 50% germination (R50) was 50 hours for Khazar and 41 hours for Hashemi, accordingly Hashemi was more uniformity in germination than Khazar. Priming treatment with KCl created the highest electrical conductivity. The minimum time duration to reach to 5, 10 and 50% of germination with 13, 16 and 32 hours, respectively was related to hydro-priming treatment. Also, the highest germination rate was related to hydro-priming. Minimum required time duration until reach to 95% germination with 66 hours was calculated in hydro-priming of Hashemi. Most plantlet establishment on 20th day after cultivation in field was related to without priming-treated seed. Hydro-priming treatment is recommended to user due to low cost and positive effectiveness on acceleration and uniformity of germination in Hashemi.
Sajad Alipour; Ali Tehranifar; Mahmoud Shoor; Leila Samiei; Homayoun Farahmand
Abstract
Fritillaria raddeana Regel is one of the Iranian-native bulbous plants well known for its medicinal and ornamental values. As regards germination and establishment are two key points in order to protect the natural populations of this species, it is important to study about propagation and domestication ...
Read More
Fritillaria raddeana Regel is one of the Iranian-native bulbous plants well known for its medicinal and ornamental values. As regards germination and establishment are two key points in order to protect the natural populations of this species, it is important to study about propagation and domestication of F.raddeana. An experiment was conducted to study the status of seed dormancy to break it in a randomized completely design including 5 priming treatment with 7 replicate in 2015- 2016. Priming treatments were arginine; in concentrations of 5 and 10 mM), gibberellic acid; concentration of 250 and 500 mg per liter, and control (without priming). All events throughout germination process seed was recorded graphically for an image. The results showed that seed priming with arginine and gibberellic acid improved germination properties. Among all treatments, arginine 5 mM has greatest impact on the rate and percentage of germination as far as it increased percentage and rate germination by 1.5 and 2.5 fold in companion with control, respectively. However, there is no significant different between arginine 10 mM and two concentration of gibberellic acid in germination results. The study on the growth of the embryo showed that F.raddeana seeds have after-ripening, so that embryo size is third of its actual size in natural habitat that gradually developed with receiving cold treatment.
hamid reza javanmard; hossein mokhtari karchegani
Abstract
In order to investigation the effects of different concentrations of leaf aqueous extracts of walnut on seed germination and seedling growth of barley, arugula and corn, a laboratory experiment were conducted in the Faculty of Agronomy, Islamic Azad University of Isfahan, in 2013. In this experiment, ...
Read More
In order to investigation the effects of different concentrations of leaf aqueous extracts of walnut on seed germination and seedling growth of barley, arugula and corn, a laboratory experiment were conducted in the Faculty of Agronomy, Islamic Azad University of Isfahan, in 2013. In this experiment, walnut leaf extract including control (distilled water), 25, 50, 75 and 100% were studied to determine juglone sensitivity in three species based on completely randomized design (CRD) with four replications. Chromatography analysis showed that a highest rate of juglone was observed on walnut leaves in August. Increasing concentration of leaf extract lead to continuously decrease in germination percentage and germination rate, root and shoot length and seedling dry weight. Germination percentage of corn and arugula seeds on primary growth stage showed resistant to walnut allelopathy. The lowest germination percentage was observed on barley in %100 leaf aqueous extracts of walnut. Corn and arugula were more sensitive to walnut extracts than barley seeds, however, root and shoot dry weight in barley had no significantly different between treatments. The aqueous extract of walnut did not have a significant effect on germination components of barley, corn and arugula, so that corn and arugula in the early stages of germination and barley in seedling growth showed strong resistance. The insensitivity of the studied species showed that the aqueous extract of Iranian walnut leaves is a strong substance in controlling weeds of these species towards sustainable agriculture.
vahid amiri monfared; Reza Tavakkol afshari; arash mamedi
Abstract
The prediction of seed viability is important for seed producers, and its prediction in storage depends on the understanding of the quantitative relationships between seed loss, seed moisture and storage temperature. In order to study the effect of temperature, seed and time on the deterioration of the ...
Read More
The prediction of seed viability is important for seed producers, and its prediction in storage depends on the understanding of the quantitative relationships between seed loss, seed moisture and storage temperature. In order to study the effect of temperature, seed and time on the deterioration of the anise seed, and also quantitative characterization of this effect and determining the coefficients of life, this study was carried out in a randomized complete block design in a factorial experiment in the Agricultural Laboratory of Tehran University. The temperature was measured at 4 levels (5, 15, 25 and 35 o C), soil moisture contents in 4 levels (5, 9, 13 and 17%) and six times (one, two, three, four, five and six months) as the factors were this test. The results of analysis of variance showed that the effect of temperature on seed moisture content was significant for germination indices at 1% level. With increasing temperature and humidity, germination declined over time. Life coefficients after six months of storage showed that survival curves can be plotted with a single source. Also, the results showed that by increasing the moisture content at any temperature, especially at higher temperatures, the life expectancy decreases with increasing moisture content. Using the life-time equation, the coefficients KE=4/21, CW =1-66, CH =0 038/0 and CQ =0,00039 were calculated.
A. Dehshiri
Abstract
Coriander seed (Coriandrum sativum L.) has essential oil, lipid and protein, and the lack attention to seed storage condition causes physical and physiological damage and ultimately decreasesits quality and deterioration so a factorial experiment in a completely randomized design was carried out and ...
Read More
Coriander seed (Coriandrum sativum L.) has essential oil, lipid and protein, and the lack attention to seed storage condition causes physical and physiological damage and ultimately decreasesits quality and deterioration so a factorial experiment in a completely randomized design was carried out and six levels of time storage (30, 60, 90,120,150 and 180 days), five level of seed moisture (5, 7, 9, 11 and 13 %) and four level of temperature (10,15,20 and 25 oc ) were studied. The results showed that the moisture content of 13% had severe harmful effects on seed germination at different temperatures compared to other humidity. In 5% moisture, an increase in temperature from 10 to 25 oc had no significant effect on germination indices especially seed germination percent and it was above 90 in all temperatures. The results of different seed packaging material showed that between aluminum, fabric, paper and cellophane, aluminum has less impact on the germination indices. The conclustion that can storage coriander seeds in the moisture content of 5% and aluminum packaging at temperature bellow 25 for long-term without a significant reduction in its maintenance.
Hossein Reza Rouhi; َAli Moradi; Maryam Saman; Yasin Mohammadi; Alireza Shahbodaghlo
Abstract
In order to investigate the effect of sodium nitroprusside (NO donor) on improvement of aged pumpkin seeds under drought stress, a factorial experiment was carried out based on completely randomized design with three replications in Bu-Ali Sina University, Hamedan, Iran. Seed priming with different concentrations ...
Read More
In order to investigate the effect of sodium nitroprusside (NO donor) on improvement of aged pumpkin seeds under drought stress, a factorial experiment was carried out based on completely randomized design with three replications in Bu-Ali Sina University, Hamedan, Iran. Seed priming with different concentrations of sodium nitroprusside including 0 (hydropriming), 25, 50 and 75 µM in drought stress levels (0, -0.2, -0.46 and -0.6 MPa) was performed. Germination percentage, germination rate, seed vigor, seedling length, soluble carbohydrates, soluble proteins, memberane electrical conductivity, malondialdehyde content and antioxidant enzymes activity (catalase, ascorbate peroxidase and superoxide dismutase) were evaluated. The results showed that seed priming with different concentrations of sodium nitroprusside mitigated significant decrease of germination parameters of aged seeds under drought stress. At -0.6 MPa, seed priming with 75 µM of sodium nitroprusside increased germination rate, vigor index , soluble carbohydrates and proteins 25, 214.1, 90.8, 117.1%, as well as the activity of catalase, superoxide dismutase and ascorbate peroxidase increased by 34, 14.5, 41.4% in comparison to control, respectively. Therefore, seed priming with sodium nitroprusside specialy with 75 µM is suggested for aged pumpkin seeds by decreasing oxidative stress due to drought stress.
Nour Ali Sajedi; Bizhan ghazi nezami
Abstract
In order to seed germination induction and response of seedling growth of Primula vulgaris to different treatments of mechanical scarification and chemical pre-treatment, an experiment was carried out in completely randomized design with treatments of control, seed scarification (for 3 minute), warm ...
Read More
In order to seed germination induction and response of seedling growth of Primula vulgaris to different treatments of mechanical scarification and chemical pre-treatment, an experiment was carried out in completely randomized design with treatments of control, seed scarification (for 3 minute), warm water 70 and 90˚C, priming with potasium nitrate at the rate of 2 and 4%, gibberellic acid at the concentration of 250 and 500 mg/L with three replicates in 2013. The results showed that treatments of seed scarification, warm water 70 and 90˚C and gibberellic acid of 250 and 500 mg/L increased the seedling emergence percentage compared to the control. The maximum of the daily emergence percentage recorded from gibberellic acid of 250 mg/L. Seed treatment with potasium nitrate of 2 and 4% decreased the mean emergence time by 61.2 and 51.7% compared with the control, respectively. The highest emergence rate coefficient equal to 0.094 and 0.092 was achieved from seed treatment with potasium nitrate of 2 and 4% .The maximum seedling length (5.53 cm), seedling dry weight (0.029 g), seedling vigor weight index (1.15) was obtained at gibberellic acid of 250 mg/L treatment. Treatments of seed scarification, warm water 70 and 90˚C, gibberellic acid at the concentration of 250 and 500 mg/L increased seedling vigor weight index compared to the control, significantly. In general, seed pre-treatment of Primula vulgaris with gibberellic acid of 250 mg/L was improved germination and seedling growth indices.
atfeh hoseini; ali moradi; yaqoub behzadi
Abstract
To study the germination of seeds of anise harvested from the mother plant under different fertilizer treatments experiment completely randomized design with 17 treatments and 4 replications was conducted in the laboratory of seed technology of Yasouj university. Treatments were concluded: Pure vermicompost ...
Read More
To study the germination of seeds of anise harvested from the mother plant under different fertilizer treatments experiment completely randomized design with 17 treatments and 4 replications was conducted in the laboratory of seed technology of Yasouj university. Treatments were concluded: Pure vermicompost (0, 5, 10 ton. ha-1) and bio fertilizers Azotobacter (Barvar-1), biological phosphorus (Barvar-2) and mixed of Barvar-1and Barvar-2, chemical nitrogen, bio fertilizers of nano and mixing treatments of vermicompost 2.5, 5, 7.5 tons. ha-1 with biological fertilizers Barvar-1, 2 and mixing of Barvar-1, 2. Results showed that the maximum α-Amylase activity was obtained by applying 7.5 tons. ha-1 vermicompost with Barvar-1, 2 and urea. The minimum values α-Amylase activity was obtained from the control treatment. The maximum percentage (92%) and rate (2.07 seed per day) of germination was belonged to the urea treatment. The maximum Seedling Length vigor index was observed in integrated treatment using organic fertilizers and biological value (9.73), showing no significant difference with urea fertilizer (9.71). Finally these results showed that the use of vermicompost and bio-fertilizers, particularly of 10 tons per hectare with the combined application of phosphate solubilizing bacteria and nitrogen stabilizer Could be as a alternative treatment for increase the absorption of nutrients such as nitrogen and phosphorus through seed germination, leading to an improvement of the germination index in anise plant.
Mohammad Entesari; Behnam Kamkar; Farshid Ghaderifar; Masoud Ahmadzadeh
Abstract
To evalute the effect of Trichoderma fungus on potato productin in the presence of Rhizoctonia solani, a three replicated greenhouse experiment was conducted in a randomized complete block design. Treatments were three strains of Trichoderma (i.e. Trichoderma harzianum (TR), Trichoderma virens (Tr2), ...
Read More
To evalute the effect of Trichoderma fungus on potato productin in the presence of Rhizoctonia solani, a three replicated greenhouse experiment was conducted in a randomized complete block design. Treatments were three strains of Trichoderma (i.e. Trichoderma harzianum (TR), Trichoderma virens (Tr2), Trichoderma atroviride (Tr3)), Rhizoctoniasolani Ag3 fungus and Agria and Sante varieties of potato provided by the seed and plant certification and registration institute. Root and shoot dry weight, stolon dry weight and number, tubers number and fresh weight were measured. the results showed that Trichoderma tretmeant could have a significant effect on the traits in the presence of fungus (pathogen-infected condition). Also, the virulence was significant decreased as compared to the contorol. Tr1 - Agria variety combined treatment had mor significant effect on the measured traits than the other combined treatments, so that dry weight of shoot and stolons, fresh weight of tubers and the number of tubers was the highest in Tr1 tretmeant which was increased as compared to the control as 80, 52, 70 and 175 percentage, respectively. Also Tr1 Treatment increased Antioxidant enzyme activity and proline content, while decreased lipid peroxidation.
saeed amini; Mohammad Hassan assarah; Abbas Dehshiri; Shahla Hashemi Fesharaki
Abstract
The main goal of seed storage is to maintain its quality from harvesting to sowing time. Among all factors, storage temperature and seed moisture content are the most important factors affecting seed longevity. This experiment was conducted at University of Tehran, Department of Agronomy and Plant Breeding ...
Read More
The main goal of seed storage is to maintain its quality from harvesting to sowing time. Among all factors, storage temperature and seed moisture content are the most important factors affecting seed longevity. This experiment was conducted at University of Tehran, Department of Agronomy and Plant Breeding during 1394 to determine the Ellis and Roberts deterioration model of Satureja hortensis seeds and introduce constants of viability equation. Seed viability and initial moisture content was measured and after that seeds were adjusted to 5, 7, 9, 11 and 13% moisture content and sealed hermetically in Nano packets. Storage temperatures were 10, 15, 20 and 25˚C. The interval of sampling depended on the storage conditions. Seed viability constants were estimated to predict seed longevity in this species and relationship between sigma and moisture content and temperatures was determined. After probit analysis, survival curves were depicted in each condition. Results showed that seeds with 5% moisture content stored at 10°C had the highest germination percentage, but seeds which were stored at 13% moisture content and 20 and 25°C had the highest deterioration rate. Estimates of KE, CW, CH and CQ were 4.31, 1.83, 0.031 and 0.0004, respectively. Also, the results showed that seed longevity decrease with increased seed moisture and temperature.
mehdi ahmadyousefi; Behnam Kamkar; Javid Gherekhloo
Abstract
This experiment was conducted as split – plot factorial based on randomized complete block design with three replications to determine the changes in maize’s seed quality on the parent plant at Jiroft's Nemooneh Field in 1395. The main factor was four levels of fertilizers (NPK) respectively ...
Read More
This experiment was conducted as split – plot factorial based on randomized complete block design with three replications to determine the changes in maize’s seed quality on the parent plant at Jiroft's Nemooneh Field in 1395. The main factor was four levels of fertilizers (NPK) respectively representing the control, 30%, 70%, and 100% of recommended value) and biochar (respectively representing no biochar and biochar application), and two strains of Trichoderma (respectively representing Trichoderma atroviride and Trichodema harzianum) were assigned to sub plot. To assess the quality of maize seed (SC.704 hybrid) produced on the parent plant, seed vigor tests (cold test and accelerated ageing test) and germination test (normal seedling percentage, germination rate, seedling length and weight) were performed. The results showed that utilization of biochar, Trichoderma strains and fertilizer levels significantly affected normal seedling percentage, germination rate, and seedling length dry weight in standard germination, cold and accelerated ageing tests. The highest seed quality based on standard germination, cold and accelerated ageing tests was obtained via application of biochar, fungi Trichoderma (Trichodema harzianum) and 70% of the amount of fertilizer recommended for the region. According to the results obtained from standard germination and seed vigor tests (cold and accelerated ageing tests), using biochar, different fertilizer levels and fungus type leads to enhanced quality of seeds produced on maternal plant in comparison to control.
Ameneh Akbarzadeh Sharafi; Hamid Reza Eisvand; N. Akbari; D. Goodarzi
Abstract
In order to study effects of seed priming with Gibberellin (GA), Ascorbic acid (AsA) and Salicylic acid (SA) on mitigation of seed deterioration damage and improvement of seed and seedling qualities of three sunflower cultivars (Farrokh, Barzegar and Shamshiri), a pot experiment was done in 2016. This ...
Read More
In order to study effects of seed priming with Gibberellin (GA), Ascorbic acid (AsA) and Salicylic acid (SA) on mitigation of seed deterioration damage and improvement of seed and seedling qualities of three sunflower cultivars (Farrokh, Barzegar and Shamshiri), a pot experiment was done in 2016. This research was carried out as a factorial experiment based on RCBD with three replications. Seeds were exposed to accelerated aging then primed. Priming treatments were included: control, hydropriming, GA (50 and 75 ppm), AsA (100 and 150 ppm), SA (138 and 207 ppm), GA 50 ppm + AsA 100ppm, GA50 ppm + SA 138 ppm, AsA 100ppm + SA 138ppm, and GA 50ppm + AsA 100ppm + SA 138 ppm. Barzegar cultivar had better seedling emergence percentage and rate than the Shamshiri and Farrokh. Seedling emergence was improved by priming treatments; however, suitable treatments were different for the cultivars. Maximum seedling emergence percentage was obtained in Barzegar by GA 50ppm + SA 138ppm; however in Shamshiri and Farrokh by hydropriming. Priming improved seedling emergence rate of the three cultivars, but the response to priming was cultivar specific. Priming decreased seedling malondialdehyde (MDA) significantly in three cultivars. The lowest MDA content was obtained in Barzegar cultivar primed by AsA + SA.
Abstract
Carrot is a particularly difficult crop to manage in terms of weed control. For weed management of carrot, seed germination is a key process because it determines both the number of weeds that could potentially emerge and the timing of their appearance in the carrot. This study was done to evaluate two ...
Read More
Carrot is a particularly difficult crop to manage in terms of weed control. For weed management of carrot, seed germination is a key process because it determines both the number of weeds that could potentially emerge and the timing of their appearance in the carrot. This study was done to evaluate two nonlinear regression models (Intersected-lines and Dent-like) to describe response of germination rate to temperature in carrot (Daucus carota L.), common chickweed (Stellaria media (L.) Vill.), yellow foxtail (Setaria glauca (L.) P. Beauv.) and canada fleabane (Conyza canadensis (L.) Cronq.). This experiment was based on completely randomized design with 4 replications at Islamic Azad University, Science Research Branch, in 2015. The seeds were treated with different temperatures (2, 5, 10, 15, 20, 25, 30, 35, 40 and 45oC). The analysis of variance showed that temperature had a significant effect on all seed germination percentage and germination rates. Intersected-lines model was superior in carrot and Dent-like model was superior for common chickweed, yellow foxtail and canada fleabane. Base, optimum and maximum temperatures were predicted with appropriate model. Base, optimum and maximum temperatures were for carrot 1.67, 22.84, 43.16; common chickweed 3.58, 18.82-19.67, 42.75; yellow foxtail 14.17, 33.75-34.92, 44.86 and canada fleabane 13.74, 31.73-31.94, 44.21ºC, respectively. This results showed that carrot germinated earliest among the studied species, because it had the lowest base temperature, so sooner planting it was caused sooner carrot establishment and less weed competition.
Mahmood Attarzadeh; Hamidreza Balouchi; Mohsen Movahhedi Dehnavi; amin salehi; Majid Rajaie
Abstract
Reduction in germination and growth can be seen in seeds obtained from mother plants which have experienced water stress. From the other point of view utilization of biological fertilizers under environmental stress, the condition can produce seeds with higher germination capacity. This research was ...
Read More
Reduction in germination and growth can be seen in seeds obtained from mother plants which have experienced water stress. From the other point of view utilization of biological fertilizers under environmental stress, the condition can produce seeds with higher germination capacity. This research was conducted as split plot based on a randomized complete block design with three replications in 2016. The main factor included of irrigation regimes at three levels included in soil irrigation after 25, 50 and 75% of soil moisture depletion and a sub-factor of phosphorus supply in six levels included of 100% phosphorus requirement from the source of triple superphosphate, 50% phosphorus + arbuscular mycorrhizal fungus, Mycorrhiza arbuscular fungus, 50% phosphorus + Pseudomonas fluorescens, Pseudomonas fluorescens and a control test without phosphorus fertilizer. Results showed that in 75% of soil moisture depletion, maximum amounts of germination, seed vigor, root length, stem length and seedling dry weight were obtained from 50% of phosphorus + arbuscular mycorrhiza. At irrigation level of 50 and 75% moisture depletion, the application of 50% phosphorus + arbuscular mycorrhiza made an increase in germination rate and uniformity. The electrical conductivity of Echinacea purpurea seeds showed increasing trend with intensification in drought stress. The results of this study showed that in present study Pseudomonas fluorescens had lower improvement effects on percent and rate of germination and the adjustment of drought adverse effects in compared with arbuscular mycorrhiza.
Javad Tabatabaeian; Azam Kadkhodaie
Abstract
Mountain celery with scientific name of Kelussia odoratissima Mozaff is one of native medicinal plants of Iran that is an endangered species due to illegal removal. Present study was carried out to find the suitable treatment for stimulating germination of seeds this plant. A factorial experiment based ...
Read More
Mountain celery with scientific name of Kelussia odoratissima Mozaff is one of native medicinal plants of Iran that is an endangered species due to illegal removal. Present study was carried out to find the suitable treatment for stimulating germination of seeds this plant. A factorial experiment based on a completely randomized design with four replications was used. Stratification (4 ̊C for 0, 4, and 8 weeks), 500 ppm solutions of Gibberellin (GA), Benzyl adenine (BA), Naphthalene acetic acid (NAA), Indole butyric acid (IBA) and combinations of these hormones as follows: (GA+IBA), (GA+ NAA), (GA+BA), (IBA+NAA), (IBA+BA), (NAA+BA), (GA+ IBA+NAA), (GA+ IBA+BA), (GA+NAA+BA), (IBA+NAA+BA), (GA+ IBA +NAA+BA) were the treatments. The results showed that Stratification or hormonal treatments alone increased germination parameters, however, 8 weeks stratification and GA were the most effective treatments. The highest germination percent and duration were obtained in GA, BA, GA+IBA+IBA and IBA after eight weeks of stratification at 4 ̊C due to treatments interaction suggesting that seeds of this species has physiological dormancy. There was no synergic effect between hormonal treatments. The results showed that mountain celery had physiological dormancy and treatments with the greatest degree of success in breaking dormancy were GA, BA, GA+IBA+BA, BA and IBA under 8 weeks stratification.
Samin Lotfi; Mokhtar Ghobadi; Saeid Jalali Honarmand; mohammad Eghbal Ghobadi
Abstract
Percentage and rate of seed germination in laboratory are usually different from field, especially under rainfed conditions. Therefore, to assessment of standard germination test to predict seedling emergence and establishment of lentil, two separate experiments were conducted under laboratory and farm ...
Read More
Percentage and rate of seed germination in laboratory are usually different from field, especially under rainfed conditions. Therefore, to assessment of standard germination test to predict seedling emergence and establishment of lentil, two separate experiments were conducted under laboratory and farm conditions. Eight lentil seed lots (includes four cultivars Kimia, Bilesavar, Qazvin and Local with different storage periods) were evaluated by the standard germination test and the rainfed farm and some indices related to seedling emergence (includes seedling emergence rate, mean time to emergence, mean daily emergence and final emergence percentage) were measured. The results of the laboratory and the farm tests showed that the seed lots were different significantly in terms of seed and seedling vigor characteristics. The correlation coefficients illustrated that the final germination percentage, the normal seedling percentage and the seed storage usage in laboratory test had positive correlations with the seedling emergence percentage in the farm experiment. The mean daily germination and the germination rate in the laboratory test had the positive correlations with the seedling emergence rate in the farm test. So it seems that, the above mentioned traits in standard germination test can use to predict seedling emergence of lentil in rainfed farm.
zeinab taghizadeh; hossein sabouri; Hossein Hosseini, Moghaddam; Fallahi Hossein Ali; Mahnaz Katouzi
Abstract
Seed germination is a manifest characteristic of the standard germination test of a cultivated variety. In order to identify genomic regions associated with seed germination, 103 family of barley in F3 and their parents Badia and Kavir were cultivated in 2015-2016, under normal and drought stress conditions. ...
Read More
Seed germination is a manifest characteristic of the standard germination test of a cultivated variety. In order to identify genomic regions associated with seed germination, 103 family of barley in F3 and their parents Badia and Kavir were cultivated in 2015-2016, under normal and drought stress conditions. Different traits including Plumule length, Radicle number, Cleoptil length, Radicle Dry weight, Plumule Dry weight and germination speed were measured. In this study, 28 pairs of microsatellite primers were used to determine the parental polymorphism. QTL analysis using composite interval mapping (CIM) for each trait in each environment for all studied traits. In general, twenty seven QTLs were detected for all traits. Total phenotypic variance explained by these QTLs varied from 8.6 to 37.3 percent. The highest LOD value for QTL controlling Radicle number (LOD=10.45) was obtained on chromosome 4 qNR-4a -15 in drought stress The results revealed that QTLs of Radicle number had desirable performance in all two environments. However, detected stable and cluster QTLs may be used in marker assisted selection (MAS) programs.
Hamidreza Naseri; Seyed Alireza Hosseini; Nateq Lashkari Sanami
Abstract
Water deficiency is one of the important problems in arid and semi-arid regions. Thus, in germination and growing stages selection of resistant medicinal plant species is very important. In this investigation in order to seed dormancy breakage of Dorema ammoniacum seeds, wet cooling treatment was assessed ...
Read More
Water deficiency is one of the important problems in arid and semi-arid regions. Thus, in germination and growing stages selection of resistant medicinal plant species is very important. In this investigation in order to seed dormancy breakage of Dorema ammoniacum seeds, wet cooling treatment was assessed first. After selection of the best cooling treatment (5 °C), treated seeds were placed in germinating medium with drought stress; at a period of 20 days at 15 and 20 °C. Drought stress trial was conducted at 5 levels as factorial layout based on a completely randomized design including control, -4, -8, -12, and -16 bar treatments. PEG was used to make drought stress. Results showed germination rate and percentage, radicle length, and stem length were significantly (p < 0/01) decreased by increasing drought stress. The best range for germination in conditions of without stress was to -12 bar water potential. It appears that stem length was more vulnerable than other characters. In addition, 20 °C was the most appropriate temperature for germination under drought treatment.
Ahmad Afkari
Abstract
In order to investigate the effects of seed inoculation with arbuscular mycorrhizal fungi on some chemical characteristics and seed germination of corn (Zea mays L.) under drought stress conditoins, a factorial experiment based on randomized complete design with three replications was conducted at the ...
Read More
In order to investigate the effects of seed inoculation with arbuscular mycorrhizal fungi on some chemical characteristics and seed germination of corn (Zea mays L.) under drought stress conditoins, a factorial experiment based on randomized complete design with three replications was conducted at the physiology laboratory of Islamic Azad University Branch Ardabili, during 2015. Studied factors were: drought stress in four levels (Zero, -4, -8 and -12 bar) and seed inoculation with mycorrhizal fungus in four levels (no inoculation, seed inoculation with Funneliformis mosseae, Rhizoglomus fasciculatum and Claroideoglomu etanicatum. The results showed that the effect of drought stress and seeds inoculation with mycorrhizal fungi on germination components, seed protein content and antioxidant enzymes was significant at 1% probability Level. Seed inoculation with Rhizoglomus fasciculatum showed higher antioxidant enzymes activity and higher seed protein content compared with inoculation with Funneliformis mosseae, Claroideoglomus etanicatum and no inoculation. In drought stress conditions, plants will reduce oxidative stress leads to increased activity of antioxidant enzymes has been. The results of the mean comparison of data showed that with increasing drought stress germigation percen and germination rate index decreased and the means of germination time, increased. With respect to the results of this study, it seems that mycorrhiza fangi by an improvement in water and nutrient absorption, improve plant growth in drought stress condition. Thus, in order to increasing of germination components and chemical characteristics, it can be suggested that seeds inoculation of corn was applied with Rhizoglomus fasciculatum
M.A. Askari Sarcheshmeh; Z. Moradi Shakoorian; M. Delshad; R. Tavakkol Afshari
Abstract
In order to study the effect of priming on onion seed germination of Zangan Cultivar in different temperature conditions, this study was carried out with 6 levels of temperature of 5 to 30 ° C and three seed pretreatment, include control, hydro-priming and halo-priming with 0.5% potassium nitrate ...
Read More
In order to study the effect of priming on onion seed germination of Zangan Cultivar in different temperature conditions, this study was carried out with 6 levels of temperature of 5 to 30 ° C and three seed pretreatment, include control, hydro-priming and halo-priming with 0.5% potassium nitrate with three replicate. The results showed that priming treatment with potassium nitrate (0.5%) could be effective on germination components. Primed seeds with potassium nitrate (0.5%) showed more germination at all temperatures. Potassium nitrate (0.5%) treatment also affected the germination reaction to temperature. These seeds showed 33.6% more germination than the other two treatments at 5°c. The results showed that germination percentage at 20°c in all treatments reaches its maximum and then remained relatively constant. In evaluating the segmented model for predicting cardinal temperatures, seed germination at base, optimum and ceiling temperatures was 1.7, 27.38, 46.09°c(in potassium nitrate (0.5%) treatment), 1, 25, 43°c (in hydro-priming treatment) and 1.9, 27.6, 46.12°c (in control treatment). For predicting time of germination at different constant temperatures used Thermal-time that constant coefficient of Thermal-time was 1872.79(°Ch).