Document Type : Original Article

Authors

1 Department of Desert Management, International Desert Research Center, University of Tehran

2 Department of Range and Watershed Management, Faculty of Natural Resources, University of Hormozgan

3 Department of Range Management, Faculty of Natural Resources, Sari Agricultural Sciences and Natural Resources University

Abstract

Water deficiency is one of the important problems in arid and semi-arid regions. Thus, in germination and growing stages selection of resistant medicinal plant species is very important. In this investigation in order to seed dormancy breakage of Dorema ammoniacum seeds, wet cooling treatment was assessed first. After selection of the best cooling treatment (5 °C), treated seeds were placed in germinating medium with drought stress; at a period of 20 days at 15 and 20 °C. Drought stress trial was conducted at 5 levels as factorial layout based on a completely randomized design including control, -4, -8, -12, and -16 bar treatments. PEG was used to make drought stress. Results showed germination rate and percentage, radicle length, and stem length were significantly (p < 0/01) decreased by increasing drought stress. The best range for germination in conditions of without stress was to -12 bar water potential. It appears that stem length was more vulnerable than other characters. In addition, 20 °C was the most appropriate temperature for germination under drought treatment.

Keywords

Abdi, H., M.R. Bihamta, E. Azizov, and R. Chogan. 2015. Investigation of drought stress levels of PEG 6000 on seed germination components and its relationship with drought tolerance indices in promising lines and cultivars of bread weheat (Triticum aestivum L.). Iranian J. Field Crops Res. 12(4): 582-596 (In Persian, with English Abstract)
Abdul-Baki, A.A., and J.D. Anderson, 1973. Relationship between decarboxylation of glutamic acid and vigor in soybean seed. Crop Sci.13: 227–232.
Alijanpour, B., P. Babakhanlou, F. Ajir, and R. Habibi. 2006. Determination of the optimal cooling time and planting depth for Dorema ammoniacum D. Don.  Iranian J. Med. Aromat. Plants Res. 21(4): 517- 534 (In Persian, with English Abstract)
Amoaghaei, R. 2007. The effect of GA3 and moist-chilling on seed dormancy breaking of Ferula ovina Bois. JCCP. 11(40): 471-482(In Persian with English Abstract)
Askarian, M. 2004. The effects of salinity and dryness on germination and seedling establishment of Kochia prostrate and Elymus junceus. Pajouhesh-va.Sazandegi. 64:71-77. (In Persian with English Abstract)
Ashraf, M., and P. J. C. Harris, 2005. Abiotic Stresses: Plant Resistance through Breeding and Molecular Approaches. The Haworth Press, New York.
Baghdadi, E., A.A. Jafari, M.A. Alizadeh, and A.H. Gorji. 2013. Effect of drought stress and cold treatment on germination and seedling growth of Poa pratensis and Poa trivialis under germination and greenhouse condition. Iranian J. Rangeland Desert Res. 20(4): 706-718 (In Persian with English Abstract)
Blum, A. 2005. Drought resistance, water-use efficiency, and yield potential-are they compatible, dissonant, or mutually exclusive. Australian J.  Agric. Res. 56: 1159-1168.
De, R., and R.K.  Kar, 1995. Seed germination and seedling growth of Mung bean (Vigna radiata) under water stress induced by PEG-6000. Seed Sci. Technol. 23(2): 301–308.
Geravandi, M., E. Farshadfar, and D. Kahrizi. 2010. Evaluation of drought tolerance in bread wheat advanced genotypes in field and laboratory conditions. Iranian J. Seed Plant Impro. 26-1 (2): 233-252 (In Persian with English Abstract)
Ghasemi arian, A.R., J. Izadi, M.R. Afkham Alshoara, and R. Ejlali. 2009. Germination improvement in seeds of gum ammoniac Dorema ammoniacum. Iranian J. Rangeland Desert Res. 15(4): 455- 463(In Persian with English Abstract)
Hermy, M., O. Honnay, L. Firbank, C. Grashof-Bokdam, and J. E. Lawesson, 1999. An ecological comparison between ancient and other forest plant species of Europe, and the implications for forest conservation. Biol Conserv. 91(1): 9-22.
Hunter, E.A., C.A. Glasbey, and R.E.L. Naylor, 1984. The analysis of data from germination tests. Journal of Agricultural Science. Cambridge 102: 207-213.
Hussain, S.S. 2006. Molecular breeding for abiotic stress tolerance. Drought perspective. P Pakistan Acad Sci. 43(3):189-210.
Irvani, N., M. Solouki, M. Omidi, A. Saidi, and A.R. Zare, 2012. Seed germination and dormancy breaking in Dorema ammoniacum D., an endangered medicinal plant. Trakia J. Sci. 10(1): 9-15.
Jafari, M. 1996. Relationship between salinity and K. proceeding of second national congress of desertification and different methods of combat desertification, N (175). Iranian Research Institute of Forest and Rangelands Publishers. Iran.
Khan, M.A., and I.A. Ungar, 2001. Seed germination of Triglochin maritima as influenced by salinity and dormancy relieving compounds.Biol. Plant. 44(2): 301-303.
Marchner, H. 1995. Mineral Nutrition of Higher Plants. Second ed. Academic Press. United States.
Matthews, S., and M. Khajeh-Hosseini, 2007. Length of the lag period of germination and metabolic repair explain vigor differences in seed lots of maize (Zea mays). Seed Sci. and Technol. 35(1): 200-212.
Moazen, M, and R. Najafi. 2013. Introduction and evaluation of the Therapeutic effects of Aloe Vera Proceedings of the First National Conference on Medicinal Plants and Sustainable Agriculture. 10 Oct 2013. Hamedan. Iran.
MurilloAmador, B., R. LopezAguilar, C. Kaya, J. LarrinagaMayoral, and A. FloresHernandez, 2002. Comparative effects of NaCl and polyethylene glycol on germination, emergence and seedling growth of cowpea. J. Agron. Crop Sci. 188(4): 235-247.
Naghizadeh, M, and R. Kabiry. 2017. Effect of salicylic acid on some physiological characteristics of corn (Zea mays L.) under dry stress. Environ. Stresses. Crop. Sci. 9(4): 315-327. (In Persian)
Ramzani-Gasak, M., M. Taghvaee, M. Masudi, A. Riahi, and N. Behbahani. 2008. Evaluation of drought and salinity effects on Capparis sninosal L. germination and growth. J. Rangeland 4: 411- 420. (In Persian)
Saeidi, M., A. Ahmadi, K. Postini, and M.R. Jahansooz. 2007. Evaluation of germination traits of different genotypes of wheat in osmotic stress situation and their correlations with speed of emergence and drought tolerance in farm situation. J. Water Soil Sci. 11(1): 281-294.
Safavi, y. 2010. Effects of drought stress on germination characteristics and seeding growth of Agropyron tauri and Agropyron repens under germinator and greenhouse conditions (In Persian). Msc. Thesis. Islamic Azad Univ. Borujerd, Iran. (In Persian, with English Abstract)
Salehi Shajani, P., M. Izadpanah, L. Fallah, M. Ramezani Yeganeh, L. Rasoulzadeh, A. Kavandi, and F. Sardabi. 2015. Comparison of the Effect of Drought Stress on Osmotic, Peroxidase, Polyphenol Oxidase and Pigment Adjustment in Different Seed Species of Anthemis tinctoria and Tripleurospermum servanes of the Iranian Natural Resources Gene Bank. J. Plant Res. 28(1): 126-139. (In Persian, with English Abstract)
Seefeldt, S.S., K.K. Kidwell, and J.E. Waller. 2002. Base growth temperatures, germination rates and growth response of contemporary spring wheat (Triticum aestivum L.) cultivars from the US Pacific Northwest. Field Crops Res. 75(1): 47–52.
Seyedi, M., J. Hamzei, A. Bourbour, V. Dadrasi, and F. Sadeghi. 2013. Effect of hydro-priming on germination properties and seedling growth of the safflower (Carthamus tinctorius L.) under drought stress. J. Agron. Sci. 5(8): 63-76. (In Persian, with English Abstract)
Susiluoto, S., and F. Berninger, 2007. Interactions between morphological and physiological drought responses in Eucalyptus microtheca. Silva Fenn. 41(2): 221-233.
Trautwein, E.A., D. Reickhoff, and H.F. Erbershobler. 1997. The cholesterol-lowering effect of psyllium a source dietary fiber. Ernhurung Umschau. 44: 214-216.
Weisz, P.R., R.F. Denison, and T.R. Sinclair. 1985. Response to drought stress of nitrogen fixation (acetylene reduction) rates by field-grown Soybean. Plant Physiol. 78(3): 525-530.
Zehtabian, G.R., H. Azarnivand, and M.M. Sharifi Kashan. 2002. Effects of drought and salinity stress on (Agropyron intermedium, Avena barbata and Panicum antidotale). J. Nat. Resour. 54(4): 409-421. (In Persian, with English Abstract)