Response of germination and electrical conductivity of seeds produced by Echinacea purpurea's mother plants under the influence of biological fertilizers and drought stress

Document Type : Original Article

Authors

1 Agronomy and Plant Breeding Department, Yasouj University, Yasouj Iran

2 Agronomy and Plant Breeding Department, Yasouj University, Yasouj, Iran

3 Agronomy and Plant Breeding Department, Yasouj University

4 Soil and Water Research Department, Fars Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran

Abstract

Reduction in germination and growth can be seen in seeds obtained from mother plants which have experienced water stress. From the other point of view utilization of biological fertilizers under environmental stress, the condition can produce seeds with higher germination capacity. This research was conducted as split plot based on a randomized complete block design with three replications in 2016. The main factor included of irrigation regimes at three levels included in soil irrigation after 25, 50 and 75% of soil moisture depletion and a sub-factor of phosphorus supply in six levels included of 100% phosphorus requirement from the source of triple superphosphate, 50% phosphorus + arbuscular mycorrhizal fungus, Mycorrhiza arbuscular fungus, 50% phosphorus + Pseudomonas fluorescens, Pseudomonas fluorescens and a control test without phosphorus fertilizer. Results showed that in 75% of soil moisture depletion, maximum amounts of germination, seed vigor, root length, stem length and seedling dry weight were obtained from 50% of phosphorus + arbuscular mycorrhiza. At irrigation level of 50 and 75% moisture depletion, the application of 50% phosphorus + arbuscular mycorrhiza made an increase in germination rate and uniformity. The electrical conductivity of Echinacea purpurea seeds showed increasing trend with intensification in drought stress. The results of this study showed that in present study Pseudomonas fluorescens had lower improvement effects on percent and rate of germination and the adjustment of drought adverse effects in compared with arbuscular mycorrhiza.

Keywords

References

Abdul Baki, A.A., and J.D. Anderson. 1973. Vigor determination in soybean seed by multiple criteria. J. Crop Sci. 13: 630-633.
Amiri, M. B., P. Rezvani Moghaddam, H.R. Ehyai, J. Fallahi, and M. Aghhavani shajari. 2011. Effect of osmotic and salinity stresses on germination and seedling growth indices of artichoke (Cynara scoolymus) and purple coneflower (Echinacea purpurea). Environ. Stresses Crop Sci. 3(2): 165-176. (In Persian)
Ansari, K., A. Salehi, M. Movahedi Dehnavi, and S. Heydari. 2016. Effect of different seed priming on germination characteristics and some antioxidant enzymes activity of Echinacea purpurea . Iranian J. Seed Sci. Res. 3(3): 1-10. (In Persian)
Atarod, H., H. Irannejad, A.H. Shirani Rad, R. Amiri, and Gh. Akbari. 2012. Assessment of drought stress and planting date effects applied on original plant, on its seed electrical conductivity rate. Iranian J. Field Crops Res. 9(2): 242-247. (In Persian)
Bauma, C., W. El-Tohamy, and N. Gruda. 2015. Increasing the productivity and product quality of vegetable crops using arbuscular mycorrhizal fungi: A review. Sci. Hortic. 187: 131-141.
Berti, M., R. Wilckens, S. Fischer, and F. Hevia. 2002. Effect of Harvest Season, Nitrogen, Phosphorus and Potassium on Root Yield, Echinacoside and Alkylamides in Echinacea angustifolia L. in Chile. Acta Hortic. 576: 303-310.
Birt, D.F., M.P. Widrlechner, C.A. LaLone, L.K, Wu, J.H. Bae, A.K.S. Solco, G.A. Kraus, P.A. Murphy, E.S. Wurtele, Q.A. Leng, S.C. Hebert, W.J. Maur, and J.P. Price. 2008. Echinacea in infection. Am. J. Clin. Nutr. 87 (2): 488S-492S.
Burd, G.I., D.G. Dixon, and B.R. Glick. 2000. Plant growth promoting bacteria that decrease heavy metal toxicity in plants. Can. J. Microbiol. 46: 237–245.
Cakmakçi, R., F. Dönmez, A. Aydın, and F. Şahin. 2006. Growth promotion of plants by plant growth-promoting rhizobacteria under greenhouse and two different field soil conditions. Soil Biol. Biochem. 38: 1482-1487.
Dodd, G.L., and L.A. Donovan. 1999. Water potential and ionic effects on germination and seedling growth of two cold desert shrubs. Am. J. Bot. 86: 1146-1153.
Elouaer, M.A., and C. Hannachi. 2012. Seed priming to improve germination and seedling growth of safflower (Carthamus tinctorius) under salt stress. Euras. J. Biosci. 6: 76-84.
Farooq, M., A.Wahid, N. Kobayashi, D. Fujita, and SMA. Basra. 2009. Plant drought stress: effects, mechanisms and management. Agron. Sustainable Dev.  29(1): 185-212.
Ghassemi-Golezani, K., and S. Ghassemi. 2013. Effects of water supply on seed development and quality of chickpea cultivars. Plant Breed. Seed Sci. 67: 37-44.
Gholami ganjeh, S., A. Salehi, and A. Moradi. 2015. Effects of maternal plant nutrition on the absorption of some nutritional elements and germination characteristics of Cumin (Cuminum cyminum L.). Iranian J. Seed Sci. Technol. 4(1): 109-118. (In Persian)
Ghorchiani, M., GH. Akbari, H.A. Alikhani, M. Zarei, and I. Allahdadi. 2013. Effect of Arbuscular mycorrhizal fungi and Pseudomonas fluorescens on phosphorus fertilizer use efficiency, mycorrhizal dependence and maize yield under water deficit conditions. J. Water Soil Sci. 17(63): 123-136. (In Persian)
Hadi, H., J. Daneshian, A. Asgharzadeh, A. Hamidi, P. Jonoubi, F. Ghooshchi, and M. Nasri. 2009. Effect of free and symbiotic nitrogen-fixing bacterial co-inoculation on seed and seedling of soybean seeds produced under deficit water condition. J. Agroecol. 1(1): 53-64. (In Persian)
Hadi, H., J. Daneshian, A. Hamidi, A. Asgharzade and, R. Zarghami. 2010. Effect of rhizobacteria on seedling characteristics of seeds produced under deficit irrigation. Agron. J. (Pajouhesh and Sazandegi). 86: 42-50. (In Persian)
Hafeez, F.Y., M.E. Safdar, A.U. Chaudry, and K.A. Malik. 2004. Rhizobial inoculation improves seedling emergence, nutrient uptake and growth of cotton. Aust. J. Exp. Agric. 44: 617-622.
Hamidi, A., J. Daneshian, and A. Asgharzadeh. 2016. A review of drought stress on mother plant effect on soybean seed germination and vigour improvement by some beneficial soil microorganisms treatment assessment. Iranian J. Seed Sci. Res. 3(2): 109-124. (In Persian)
Han, H.S., and K.D. Lee. 2006. Effect of co-inoculation with phosphate and potassium solubilizing bacteria on mineral uptake and growth of pepper and cucumber. Plant Soil Environ. 52: 130-136.
Hobbs, C.B. 1994. Echinacea, a literature review. Herbalgram. 30: 33-47.
ISTA. 2003. Handbook for seedling evaluation (3rd.ed). International Seed Testing Association. Zurich, Switzerland.
Kafi, M., and A. Mahdavi-Damghani. 2000. Mechanism of Tolerance to Environmental Stress in Sowings Plant. The Ferdowsi University of Mashhad. (In Persian)
Larson, L. A. 1968. The effect of soaking pea seed with or without seed coats has on seedling growth. Plant Physiol. 43: 255-259.
Lazcano, C., F.H. Barrios-Masias, and L.E. Jackson. 2014. Arbuscular mycorrhizal effects on plant water relations and soil greenhouse gas emissions under changing moisture regimes. Soil Biol. Biochem. 74: 184-192.
Lifshitz, R., J.W. Kloepper, M. Kozlowski, C. Simonson, J. Carlson, E.M. Tipping, and I. Zaleska. 1987. Growth promotion of canola (rapeseed) seedling by a strain of Pseudomonas putida under gnotobiotic conditions. Can. J. Microbiol. 33: 390-395.
Makkizadeh Tafti, M., R. Farhoudi, H.A. Naghdi badi, and A. Mehdizadeh. 2006. Assigning the best treatment for increasing germination of three medicinal plants seeds: Rubia tinctourum L., Echinacea angustifolia D.C. and Myrtus communis L. Iranian J. Med. Aromat. Plants. 22 (2): 105-116. (In Persian)
Noumavo, P.A., E. Kochoni, Y.O. Didagbe, A. Adjanohoun, M. Allagbe, R. Sikirou, E. W. Gachomo, S.O. Kotchoni, and L. Baba-Moussa. 2013. Effect of different plant growth promoting rhizobacteria on maize seed germination and seedling development. Am. J. Plant Sci. 4: 1013-1021.
Omidbeigi, R. 2002. Study of cultivation and adaptability of purple coneflower (Echinaceae purpurea) in the north of Tehran. Water Soil Sci. (J. Sci. Technol. Agric. Nat. Res.). 6(2): 231-240. (In Persian)
Penrose, D.M., and B.R. Glick. 2003. Methods for isolating and characterizing ACC deaminase‐containing plant growth‐promoting rhizobacteria. Physiol. Plant. 118: 10-15.
Salar, M., H.R. Mobasser, and A. Ghanbari-Malidarreh. 2013. Effects of nitrogen and potassium rates of mother plant on seed N and K content, germination and seedling growth of rice seeds. Adv. Environ. Biol. 7(1): 147-152.
Schenck, N.C., and K. Perez. 1990. Manual for the identification of VA mycorrhizal fungi. Synergistic Publishing Gainesville, Florida, USA.
Sharma, A.K., and A.K. Sharma. 2002. Biofertilizers for Sustainable Agriculture. Agrobios, India.
Smith, S.E., and D.J. Read. 2008. Mycorrhizal Symbiosis. Third Ed. Academic Press, London.
Soltani, A., S. Galashi, E. Zeinali, and N. Latifi. 2001. Germination, seed reserve utilization and seedling growth of chickpea as affected by salinity and seed size. Seed Sci. Technol. 30(1): 51-60.
Tajik, M., I. Alahdadi, J. Daneshian, H. Iran nezhad, A. Hamidi, and H. Jabbari. 2008. Consequence of application some of biofertilizers on improvement of the soybean Glycine max (L.) Merr. Seed vigour produced under water deficit condition. Agric. Res. 7(4): 13-27. (In Persian)
Tari, I., J. Csiszár, G. Szalai, F. Horváth, A. Pécsváradi, G. Kiss, Á. Szepesi, M. Szabó, and L. Erdei, 2002. Acclimation of tomato plants to salinity stress after a salicylic acid pretreatment. Acta Biol. 46(3-4): 55-56.
Vurukonda, S.S., S. Vardharajula, M. Shrivastava, and A. Skz. 2016. Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiol. Res. 184: 13-24.

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