Mehdi Razmkhah; Ali Moradi; Hojatollah Latifmanesh; Hamidreza Balouchi
Abstract
Camelina (Camelina sativa) is a one-year oil plant, one of the most important problems of its cultivation is the interaction of seed germination and seed establishment with drought stress. Use of herbal treatments such as rye plant extract can speed up them, especially in drought-stress conditions. Therefore, ...
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Camelina (Camelina sativa) is a one-year oil plant, one of the most important problems of its cultivation is the interaction of seed germination and seed establishment with drought stress. Use of herbal treatments such as rye plant extract can speed up them, especially in drought-stress conditions. Therefore, this research was conducted to investigate the effect of priming and priming time on germination and some biochemical traits of Camelina under drought stress in the Agriculture and Genetics Laboratory of Yasouj University from 2021 to 2022. The experiment was conducted as a factorial in a completely randomized design with 4 replications. The first factor was priming with Danko variety of rye plant root extract at 3 levels (zero, 40 and 60%), The second factor was priming times at three levels (zero, 6 and 12 hours) and the third factor included drought stress at three levels (zero, -3 and -6 times). Based on the obtained results, it was observed that drought stress affected the biochemical indicators and germination of Camelina and pre-treatment with rye extract at a concentration of 60% along with prime in 12 hours with the improvement of some biochemical indicators and germination were able to increase the tolerance of Camelina seedlings under drought stress by affecting the non-enzymatic antioxidant defense system of the plant. The results of the experiment show that using Danko variety of rye root extract with a concentration of 60% in 12 hours improves physical characteristics of the seed, helping germination in optimal and drought stress conditions.
Parimah Shokouhi Nasab; Hamidreza Balouchi; َAli Moradi; Mohsen Movahhedi Dehnavi
Abstract
This experiment was conducted in order to investigate the effect of seed coating with biochar and activated carbon on some quinoa seed germination indicators, and it was implemented as a factorial in the form of a completely randomized design with four replications in 2019, in Yasouj University's Faculty ...
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This experiment was conducted in order to investigate the effect of seed coating with biochar and activated carbon on some quinoa seed germination indicators, and it was implemented as a factorial in the form of a completely randomized design with four replications in 2019, in Yasouj University's Faculty of Agriculture. The first factor is seed coating in four levels (no coating, coating with activated carbon, coating with biochar and coating with activated carbon + biochar) and the second factor includes salinity stress in four levels (zero, 75, 150 and 225 mM sodium chloride). The results of the interaction of salinity stress and seed coating showed that the highest content of soluble sugar (28.578 mg/g seed FW) and malondialdehyde (2.97 µmol/g seed FW) was obtained by coating with activated carbon in 150 mM salinity stress. Also, the highest amount of seed hydrogen peroxide (0.18 μmol/g seed FW) and proline (10.49 μmol/g seed FW) at the level of 225 mM sodium chloride was obtained by covering the seeds with biochar and active carbon, respectively. Covering seeds with activated carbon and biochar led to an increase in the length of root and shoot under salt stress conditions. However, under salinity stress conditions, root weight and length vigor index of seed improved more by coating seeds with activated carbon. It can be stated that seed coating can greatly reduce the harmful effects of osmotic stress on germination and biochemical traits in quinoa seedlings and improve seedling growth.
Hamidreza Balouchi; Afsaneh Bekhradyani Nasab; Mohsen Movahhedi Dehnavi; Ali Sorooshzadeh
Abstract
Saffron is propagated exclusively by corm because of sterility. Therefore, choosing the suitable corm for cultivation is one of the important factors in saffron production and the final yield depends on the size of the corm. The research was conducted to investigate the effects of benzyl aminopurine ...
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Saffron is propagated exclusively by corm because of sterility. Therefore, choosing the suitable corm for cultivation is one of the important factors in saffron production and the final yield depends on the size of the corm. The research was conducted to investigate the effects of benzyl aminopurine hormone priming, application of bio-fertilizers and maternal corm weight on the germination indices of saffron cormlets. Experimental treatments consisted of large (7.1-10 g) and small (4-7 g) corms, priming with benzyl aminopurine at concentrations of 0, 250, and 500 mg/l and biofertilizer levels (no fertilizer, Mycorrhiza (Funneliformis mossea) and fertilizer, Phosphate Barvar2). The results showed that maternal corms with 7.1 to 10 g compared to 4 to 7 g increased seedling emergence rate by 14% and the seedling vigor length index by 6.3%. Hormonal priming of maternal corms with a concentration of 500 mg/l increased dry weight leaf of main and lateral shoots (34%), dry weight of the main and total shoots (37% ), and also improved emergence rate (56%) and the number of the contractile root of cormlet (77%) with biofertilizer application. The interaction of hormonal priming (250 mg/l) and the use of maternal corms with 7.1 to 10 g increased the weight index of seedling vigor by 72%. Biofertilizer treatment increased 25% of the total dry weight of the shoots. In general, the application of larger corms and the application of biofertilizer and hormonal priming improved cormlet and saffron seedling vigor.
Navid Abbasi; Hamidreza Balouchi; Alireza Yadavi; Amin Salehi
Abstract
In order to investigate the effect of water deficit stress and sources of nitrogen fertilizer on germination quality and vigor of wheat seeds of Chamran cultivar, an experiment was conducted in Agricultural Research Center of Fars province. In this experiment, wheat seeds were first treated at three ...
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In order to investigate the effect of water deficit stress and sources of nitrogen fertilizer on germination quality and vigor of wheat seeds of Chamran cultivar, an experiment was conducted in Agricultural Research Center of Fars province. In this experiment, wheat seeds were first treated at three levels of water deficit stress (complete irrigation, water deficit stress in milky and rough stage) and four levels of nitrogen fertilizer sources (urea fertilizer 100% plant requirement, urea fertilizer 25% Along with nitroxin and nitrogen, 50% urea fertilizer with nitroxin and nitrogen, 75% urea fertilizer with nitroxin and nitrogen were produced. Then, the quality and vigor of the seeds were tested in two standard experiments of germination and accelerated aging in a factorial design with four replications. The results showed that the effect of experimental treatments on all measured traits except germination percentage was significant. Water deficit stress and sources of nitroxin and nitrogen fertilizer with 25% urea at the beginning of decline led to an increase in protein concentration (12.39%), but the lowest amount of starch (20.85 %) under the interaction of nitroxin and nitrogen fertilizer with 75% urea in 48 hours Deterioration was observed. Water deficit stress also increased the concentration of soluble sugars and decreased the grain alpha-amylase enzymes. Overall, the results of this study showed that the use of combined treatments of urea fertilizer and biofertilizers in stress conditions to feed the mother plant led to the production of seeds with higher vigor and more resistance to decay.
Sajad Argha; Hamidreza Balouchi; Ali Moradi; Alireza Yadavi
Abstract
This research was carried out to evaluate the storage of wheat seed which produced under different conditions of water deficit and application of chemical nitrogen and biological fertilizers as the first factor was water deficit in milky stage, drought stress at dough stage and without stress (control), ...
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This research was carried out to evaluate the storage of wheat seed which produced under different conditions of water deficit and application of chemical nitrogen and biological fertilizers as the first factor was water deficit in milky stage, drought stress at dough stage and without stress (control), and nitrogen fertilizer resources in Urea fertilizer 100%, 75%, 50% and 25% of plant requirement with nitroxin and nitragin. The second factor was storage conditions in 7 and 14% seed moisture within 20 and 4 °C storage, and the third factor was the storage period at 50, 100, 150 and 200 days. The results showed that the content of soluble protein and seed vigor index in seeds that received nitroxin and nitragin fertilizers with 25% urea, by increasing the water deficit, from 1.208 to 0.581 mg/g fresh weight and from 1.945 to 0.376 increased relative to 100% urea, respectively. The amount of catalase activity in seeds with drought stress and biofertilizer was higher and its activity increased at higher storage temperatures. In general, if the irrigation of the maternal plant is discontinued at the seed dough stage and the plant is fed with integrated of chemical and biological fertilizers, then the seed produced had more storage capability.
Ali Hoseinipoor; Alireza Yadavi; Hamidreza Balouchi; َAli Moradi
Abstract
Germination is one of the most sensitive stages of plant life, which is affected by various environmental factors such as temperature and humidity. Because seed standard germination test alone cannot accurately describe seedling emergence in field conditions, seed strain tests such as accelerated aging ...
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Germination is one of the most sensitive stages of plant life, which is affected by various environmental factors such as temperature and humidity. Because seed standard germination test alone cannot accurately describe seedling emergence in field conditions, seed strain tests such as accelerated aging test (seed deterioration) are used. In this research, germination and biochemical indices of linseed (Linum usitatissimum Var. Norman) in moisture potential of 0, -0.2, -0.4, -0.6, -0.8, -1.2 and -1.2 MPa under accelerated aging conditions at temperatures 10, 15, 20, 25, 30 and 35°c were evaluated. The results showed that the percentage and rate of germination and seedling vigor index in accelerated aging and non-accelerated aging respectively were increased by increasing temperature up to 20 and 25 ° C, , then decreasing to 35 ° C. And with decreasing potential water, in terms of accelerated aging, showed its lowest level at any temperature. The amount of soluble protein, catalase activity and proline content of seeds increased with decreasing water potential and accelerated aging effects at each temperature. Also, with increasing temperature, protein content and catalase enzyme activity of Linum usitatissimum seed are increased; proline content of the seed decreased.The results of this study showed that Linum usitatissimumis resistant to high and low temperatures in the early stages of germination under favorable temperature conditions of plants resistant to dehydration and under stress-free conditions.
atfeh hoseini; َAli Moradi; H.R. Balouchi
Abstract
In order to investigate seed bio-priming effects on some germination index of Pimpinella anisum L. under drought stress, this research was done in genetic lab at Agriculture department of Yasouj University. The experiment was factorial with two factor based on CRD design in 4 replications and in each ...
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In order to investigate seed bio-priming effects on some germination index of Pimpinella anisum L. under drought stress, this research was done in genetic lab at Agriculture department of Yasouj University. The experiment was factorial with two factor based on CRD design in 4 replications and in each Petri dishe 30 seeds were planted. First factor was seed bio-priming with nine level (on bio-priming, 4 Bactria strain (P.fluorescent), fluorescent (CHA0), 1) P.F (, 2) P.F (, 16) B (and 52 (B)) and 4 strain of T.harzianum (T29, T32, T43, T36 and T39) and second factor include osmotic potential of drought stress in 3 levels (0, -3 and -6 bar). The evaluated traits in this study included germination percentage, germination rate, seedling length, dry weight, seedling vigour index (weight and length) and alometric coefficient. Results of test showed that increasing drought stress, decreased seed germination indexes, and bio-priming couldn’t improve these indexes than non-priming seeds, therefore in each of drought stress potential, T36 has the maximum germination percentage (83.88), germination rate (4.7), seedling length vigor index (10), seedling dry weight (8.07), and seedling weight vigor index (15.25) and not primed seeds have the lowest amount of this traits. According to the results, it can be stated that the application of bio-priming treatment can moderately reduce the adverse effects of drought stress in the germination stage of pimpinella anisum L.
Samira Parsaie; Mohsen Movahhedi Dehnavi; H.R. Balouchi; Mahmood Attarzadeh
Abstract
Drought stress on maternal plant can negatively affect germination characteristics of produced seeds, and micronutrients application in this circumstance may mitigate drought impacts and leads to seeds with higher vigor. This research was conducted as two experiments in 2015 in seed laboratory of Yasouj ...
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Drought stress on maternal plant can negatively affect germination characteristics of produced seeds, and micronutrients application in this circumstance may mitigate drought impacts and leads to seeds with higher vigor. This research was conducted as two experiments in 2015 in seed laboratory of Yasouj University. At first experiment the seeds obtained from a former experiment included of three irrigation levels (after 75, 110 and 145 mm evaporation from class A pan) and four foliar applications included of water, zinc sulfate 3000 ppm, boric acid 2000 ppm and mixture of zinc sulfate and boric acid, were evaluated in germination experiments in 30°c. Second experiment was a factorial based on RCD with three replications. First factor was drought stress, by PEG 6000, in three levels (0, -0.2 and -0.4 Mpa), and second factor in eight levels included of seed soaking in water, ZnSo4, H3Bo3 and ZnSo4 + H3Bo3 and seed with high content of Zn , B and Zn+ B and a dry seed as control. First experiment results showed that with delaying in irrigation time, foliar application of micronutrients, compared to water foliar application, significantly increased germination traits. In second experiment, although seed soaking in nutrients, significantly decreased germination indices, but seed with high zinc and boron contents positively affected on germination vigor and antioxidant activities especially under drought stress condition.
Mahmood Attarzadeh; Hamidreza Balouchi; Mohsen Movahhedi Dehnavi; amin salehi; Majid Rajaie
Abstract
Reduction in germination and growth can be seen in seeds obtained from mother plants which have experienced water stress. From the other point of view utilization of biological fertilizers under environmental stress, the condition can produce seeds with higher germination capacity. This research was ...
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Reduction in germination and growth can be seen in seeds obtained from mother plants which have experienced water stress. From the other point of view utilization of biological fertilizers under environmental stress, the condition can produce seeds with higher germination capacity. This research was conducted as split plot based on a randomized complete block design with three replications in 2016. The main factor included of irrigation regimes at three levels included in soil irrigation after 25, 50 and 75% of soil moisture depletion and a sub-factor of phosphorus supply in six levels included of 100% phosphorus requirement from the source of triple superphosphate, 50% phosphorus + arbuscular mycorrhizal fungus, Mycorrhiza arbuscular fungus, 50% phosphorus + Pseudomonas fluorescens, Pseudomonas fluorescens and a control test without phosphorus fertilizer. Results showed that in 75% of soil moisture depletion, maximum amounts of germination, seed vigor, root length, stem length and seedling dry weight were obtained from 50% of phosphorus + arbuscular mycorrhiza. At irrigation level of 50 and 75% moisture depletion, the application of 50% phosphorus + arbuscular mycorrhiza made an increase in germination rate and uniformity. The electrical conductivity of Echinacea purpurea seeds showed increasing trend with intensification in drought stress. The results of this study showed that in present study Pseudomonas fluorescens had lower improvement effects on percent and rate of germination and the adjustment of drought adverse effects in compared with arbuscular mycorrhiza.
R. Afrosheh; Hamidreza Balouchi; M. Movahedi Dehnavi; M.H. Gharineh
Abstract
The seeds of oilseed plants are susceptible to deterioration and the seed power in oilseed plants decreases faster over the time. In order to evaluate the effect of salicylic acid and seed aging on germination characteristics and biochemical changes of Carthamus tinctorius L. cv. Soffeh seeds,an experiment ...
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The seeds of oilseed plants are susceptible to deterioration and the seed power in oilseed plants decreases faster over the time. In order to evaluate the effect of salicylic acid and seed aging on germination characteristics and biochemical changes of Carthamus tinctorius L. cv. Soffeh seeds,an experiment was conducted as a factorial base on completely randomized design with fourand three replications for germination and biochemical characteristics respectively. The first factor was priming by salicylic acid include seven levels in 0 (distilled water), 50 and 100 ppm for 24 and 48 h in 15°Cand without priming (control)and secondary factor was combinations four levels of aging (0, 2, 4 and 6 days). Results showed that the interaction of priming and aging effects on germination rate, normal seedling percentage, abnormal seedling percentage, seedling length, length of seed vigor index, seedling dry weight, weight of seed vigor index and EC were significant, but on germination percentage, total protein, catalase and ascorbate peroxidase only affected by the main effects.Results showed that germination characteristics with increasing in the aging duration were reduced significantly and using priming germination characteristics were increased but protein, catalase and ascorbate peroxidase decline along with increasing seed aging. In the study, using priming treatment salicylic acid 50 ppm for 48 h had higher germination characteristics and antioxidant activity and soluble proteins in comparison with untreated or control of safflower seeds.
R. Ostadian Bidgoly; H.R. Balouchi; E. Soltani; A. Moradi
Abstract
There is a little information about the effects of temperature and water potential on safflower. In order to study the effects of temperature and water potential on seed germination characteristics of safflower (Carthamus tinctorius L.), an experiment as combined analysis was conducted base on a completely ...
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There is a little information about the effects of temperature and water potential on safflower. In order to study the effects of temperature and water potential on seed germination characteristics of safflower (Carthamus tinctorius L.), an experiment as combined analysis was conducted base on a completely randomized design with 4 replications by 25 seeds in laboratory of seed technology at Yasouj University in 2015. The factors included temperature (5, 10, 15, 20, 25, 30, 35, 40 and 45 oC) and water potential (0, -0.2, -0.4, -0.6, -0.8, -1, -1.2, -1.4 and -1.6 MPa). The percentage and rate of germination, root length, shoot length, ratio of root length to shoot length and seedling vigor index were measured in this experiment. The results of analysis of variance showed that main effects of temperature and water potential and interactions were significant on all indicators of germination at 1% probability level. Also, by decreased water potential, germination percentage and germination rate reduced. At 5 oC and at zero potential germination percentage was 98% while the potential of -1.6 MPa germination percentage dropped to zero. At 35 oC and at zero potential germination percentage was 95% while the using potential of -1.6 MPa germination percentage decreased to 1%. By increasing the temperature up to 20oC increasing germination percentage and germination rate detected and afterwards reduced.
S. Baladi; H.R. Balouchi; A. Moradi; M. Movahhedi Dehnavi
Volume 5, Issue 1 , March 2015, , Pages 107-122
Abstract
Storage conditions, especially high moisture storage and high temperature environments, greatly affect the quality of oil seeds. In order to investigate the effect of temperature, seed moisture content and storage time on the deterioration of flax seed oil a factorial experiment was conducted based on ...
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Storage conditions, especially high moisture storage and high temperature environments, greatly affect the quality of oil seeds. In order to investigate the effect of temperature, seed moisture content and storage time on the deterioration of flax seed oil a factorial experiment was conducted based on a completely randomized design with four replications in the seed lab of Yasouj University. The factors included the temperature at four levels (15, 25, 35 and 45 °C), moisture content in 5 levels (initial moisture content, 5, 9, 13 and 17%) and six storage times (30, 60, 90, 120, 150 and 180 days). The results showed that germination and normal seedlings percentage, germination rate and vigor index reduced with increasing moisture content and during storage at each temperature level and with a combination of high temperature and moisture content this reduction was more severe. The lowest level of deterioration was in 5 °C with 5% moisture content that after 180 days storage, viability was reduced from 93 to 90 percent. Over time and with increasing moisture content, electrical conductivity also increased, and this increase was greater at high temperatures and seed lots with high electrical conductivity had low viability
