Elahe Nikouee; Maryam Mollashahi; Alireza Moshki; Homan Ravanbaksh
Abstract
Due to the fact that Elaeagnus angustifolia seed has a hard shell, the present study was conducted to investigate the types of appropriate treatments for germination of Elaeagnus angustifolia seed. The experiment was done in Semnan. Seed scarification performed in a completely randomized design with ...
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Due to the fact that Elaeagnus angustifolia seed has a hard shell, the present study was conducted to investigate the types of appropriate treatments for germination of Elaeagnus angustifolia seed. The experiment was done in Semnan. Seed scarification performed in a completely randomized design with three replications (12 seed per replication). Experimental treatments to break dormancy of Elaeagnus angustifolia seed included control, 60% sodium hydroxide for 20 and 30 minutes, 98% sulfuric acid for 20 and 30 minutes, 1% oxygenated water for 10 and 20 minutes, physical scraping, 90°C boiling water for 15 minutes and ice water(5 days at a temperature of zero degrees and then 24 hours at a temperature of 25 degrees for 3 days). The results showed that there was a significant difference at the level of 1 % between the germination treatments of Elaeagnus angustifolia seed including of germination percent, germination rate, and mean time of germination seed vitality index, and seed vegetative traits such as root length, stem length, number of leaflets and seed germination indices. The highest germination percentage was 88.67% in 98% sulfuric acid treatment for 20 minutes. Also, the highest seed vigor index (96%), mean root length(48 mm), mean stem length(60 mm) and average number of leaflets (5.7 number) were observed in this treatment. Totally, the results showed that in all parameters examined that Sulfuric acid treatment of 98% for 20 years obtained the highest values, but two treatments of ice water and boiling water prevented the germination of Oleaster seeds.
fatemeh shamsodin; alireza moshki; homan ravanbakhsh; maryam mollashahi
Abstract
This study aimed to investigate the effects of different treatments on seed germination and survival of seeds in two regions around Semnan, Sokan (as the understory of pine and black locust plantation) and Soofiabad (as a desert shrub land). The amount of germination of these seeds from two region was ...
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This study aimed to investigate the effects of different treatments on seed germination and survival of seeds in two regions around Semnan, Sokan (as the understory of pine and black locust plantation) and Soofiabad (as a desert shrub land). The amount of germination of these seeds from two region was estimated by 22 treatments through mechanical (scarification by sand paper) and chemical treatments (sulfuric acid, nitric acid, sodium hydroxide), each treatment in three period of time (10, 20 and 30 minute) and control treatment under greenhouse condition. Furthermore, some parameters such as seed germination speed, height and diameter of seedlings were studied by applying 22 different treatments. The results showed that the highest germination and growth of seedlings were observed under physical scarifying treatment and under Sulfuric acid treatment after 10 minutes. Amount of germination in mechanical treatment (scarification by sand paper) in Sokan seed (71 percent) was more than Sofi abad (57 percent), significantly. Increasing the time of sulfuric acid treatment (from 10 to 30 minute) decreased the germination of seeds (maximum germination in Sokan region in 10 minute treatment was 53.4 and in Sofiabad region was 28.3 percent). The germination rate in Sokan forest ecosystem under scarifying treatment and sulfuric acid (10 minutes) was significantly higher (53.4) than those in desert ecosystem Sufi-Abad (28.7). Moreover, the results of electric conductivity test also showed that the Sufi-Abad had lower quality of seeds. As electric conductivity in Sokan and Sofiabad seed was 3.36 and 6.84, respectively.
M. Mollashahi; A.R. Moshki; H. Ravanbakhsh
Abstract
Basswood(Tilia begonifolia) and Blacklocust (Robinia pseudoacacia) are two forest trees with seed dormancy due to very hard seed coat. Seeds of two species were collected from Ghorogh nursery in Gorgan province to remove seed coat (breaking seed dormancy) and simplify germniation process and examine ...
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Basswood(Tilia begonifolia) and Blacklocust (Robinia pseudoacacia) are two forest trees with seed dormancy due to very hard seed coat. Seeds of two species were collected from Ghorogh nursery in Gorgan province to remove seed coat (breaking seed dormancy) and simplify germniation process and examine the effects of scarification on their germination and viability in 2014.The tetrazolium test was done to measure the seed vitatlity.Then the seed germination rate was tested using hot water (100°C for 1 min. ), H2O2 (1 % for 10 min.), H2SO4 (30 % for 10 min.) and control treatments. The experiment was done in the laboratory of the Faculty of Desert Study (Semnan university). The results of tetrazolium test showed that Tilia begonifolia and Robiniapseudoacacia have 57.25 and 78.30 percent of viability, respectively. The highest rates of seed germination of Tilia begonifolia and Robinia pseudoacacia were observed in H2O2 (%38) and hot water (%66.69) treatments, respectively. As the seeds of Robinia pseudoacacia have higher seed germination than Tilia begonifolia despite of having hard seed coat, it can be concluded that other factors except of hard seed coat can affect seed germination of Tilia begonifolia. Basswood(Tilia begonifolia) and Blacklocust (Robinia pseudoacacia) are two forest trees with seed dormancy due to very hard seed coat. Seeds of two species were collected from Ghorogh nursery in Gorgan province to remove seed coat (breaking seed dormancy) and simplify germniation process and examine the effects of scarification on their germination and viability in 2014.The tetrazolium test was done to measure the seed vitatlity.Then the seed germination rate was tested using hot water (100°C for 1 min. ), H2O2 (1 % for 10 min.), H2SO4 (30 % for 10 min.) and control treatments. The experiment was done in the laboratory of the Faculty of Desert Study (Semnan university). The results of tetrazolium test showed that Tilia begonifolia and Robiniapseudoacacia have 57.25 and 78.30 percent of viability, respectively. The highest rates of seed germination of Tilia begonifolia and Robinia pseudoacacia were observed in H2O2 (%38) and hot water (%66.69) treatments, respectively. As the seeds of Robinia pseudoacacia have higher seed germination than Tilia begonifolia despite of having hard seed coat, it can be concluded that other factors except of hard seed coat can affect seed germination of Tilia begonifolia.
