Investigation of viability rate and the effects of different breaking treatments of physical dormancy on seed germination of two tree species (Basswood and Black locust)

Document Type : Original Article

Authors

1 Assistance Professor/ Faculty of desert studies, Semnan University, Semnan, Iran.

2 Assistance Professor/Ffaculty of desert studies, Semnan University, Semnan, Iran

Abstract

Basswood(Tilia begonifolia) and Blacklocust (Robinia pseudoacacia) are two forest trees with seed dormancy due to very hard seed coat. Seeds of two species were collected from Ghorogh nursery in Gorgan province to remove seed coat (breaking seed dormancy) and simplify germniation process and examine the effects of scarification on their germination and viability in 2014.The tetrazolium test was done to measure the seed vitatlity.Then the seed germination rate was tested using hot water (100°C for 1 min. ), H2O2 (1 % for 10 min.), H2SO4 (30 % for 10 min.) and control treatments. The experiment was done in the laboratory of the Faculty of Desert Study (Semnan university). The results of tetrazolium test showed that Tilia begonifolia and Robiniapseudoacacia have 57.25 and 78.30 percent of viability, respectively. The highest rates of seed germination of Tilia begonifolia and Robinia pseudoacacia were observed in H2O2 (%38) and hot water (%66.69) treatments, respectively. As the seeds of Robinia pseudoacacia have higher seed germination than Tilia begonifolia despite of having hard seed coat, it can be concluded that other factors except of hard seed coat can affect seed germination of Tilia begonifolia
Basswood(Tilia begonifolia) and Blacklocust (Robinia pseudoacacia) are two forest trees with seed dormancy due to very hard seed coat. Seeds of two species were collected from Ghorogh nursery in Gorgan province to remove seed coat (breaking seed dormancy) and simplify germniation process and examine the effects of scarification on their germination and viability in 2014.The tetrazolium test was done to measure the seed vitatlity.Then the seed germination rate was tested using hot water (100°C for 1 min. ), H2O2 (1 % for 10 min.), H2SO4 (30 % for 10 min.) and control treatments. The experiment was done in the laboratory of the Faculty of Desert Study (Semnan university). The results of tetrazolium test showed that Tilia begonifolia and Robiniapseudoacacia have 57.25 and 78.30 percent of viability, respectively. The highest rates of seed germination of Tilia begonifolia and Robinia pseudoacacia were observed in H2O2 (%38) and hot water (%66.69) treatments, respectively. As the seeds of Robinia pseudoacacia have higher seed germination than Tilia begonifolia despite of having hard seed coat, it can be concluded that other factors except of hard seed coat can affect seed germination of Tilia begonifolia

Keywords


Agboola,D.A., and M.O. Adedire .1998. Response of treated dormant seeds of three species to germination promoters.Nig. J. Bot.11: 103 – 109
Akinola, J.O., A, Larbi., G.O, Farinu and A.A. Odunsi .2000. Seed treatment methods and duration effects on germination of wild sunflower, Expl. Agric. 36:63-69.
Asgari Mehrabadi, M., M., Noori, F, Amini and F. Beigi .2012. Gemination analysis, Colorophyll content and growth of Robinia pseudoacacia and its reaction to peteroleum pollution, (In Persian, with English Abstract.) Iranian J. plant Biol. 3(7): 41-54.
Basbag, M., A, Aydin and D. Ayzit. 2010. The Effect of Different Temperatures and Durations on the Dormancy Breaking of Black Locust (Robinia pseudoacacia L.) and Honey Locust (Gleditsia triacanthos L.) Seeds, Not. Sci. Biol. 2 (4) :125-128.
Ebrahimi, A and S.V. Eslami. 2013. Effect of different treatment on breaking seed dormancy of alcolmia africana L. and Cuscuta monogynaVahl, (In Persian, with English Abstract.) J. Plant protection (Agric. Sci. Technol.). 26(2):191-198.
Forestry office. 2003. North forest species Tarif, Iran Forest and Rangeland Organization Publishing, 1:114p.
Heit C.E.1967. Propagation from seed: 7. Successful propagation of six hard seeded group species. American Nurseryman 125(12): 10-12.
ISTA. 1999. International rules for seed testing. Seed Science and Technology 27(Supplement).
Khadduri N.Y, J.T Harrington, L.S, Rosner and D.R, Dreesen. 2002. Percussion as an Alternative Scarification for New Mexico Locust and Black Locust Seeds, Forest Service, Rocky Mountain Research Station, Proc. RMRS-P-24. 370 p.
Khaleghi, E., A ,Dehghan., and N, Moalemi. 2010. Effect of H2SO4 and hot water on seed germination indexes of Tamarindus indica and Acacia sp, (In Persian, with English Abstract.) Iranian J. Hortic. Sci. 4(3):71-77(In Persian).
Kobmoo, B., O, Chichansumat and Pukitayacamee,P. 1990. A preliminary study on pretreatment of seed Legominose species. The Embryo. 3:6-10.
Lotfi, Sh., M ,Rahimi zadeh and E. Soltani. 2013. Determination of best method for seed dormancy breaking in Acroptilon repens, (In Persian, with English Abstract.) Iranian J. Seed sci.Technol. 2(4); 24-32(In Persian).
Mollashahi, M., S.M ,Hosseini., D, Bayat., B , Naseri., A, Rezaee and L. Vatani. 2009. Effect of seed time collection on seed vigor and vitality of Tilia begonifolia, (In Persian with English Abstract) Iranian J. Forest and poplar Res. 16(3): 478-485.
Moshki, A and N.P. Lamersdorf. 2011. Growth and Nutrient Status of Introduced Black Locust (Robinia pseudoacacia L.) Afforestation in Arid and Semi Arid Areas of Iran. Res. (In Persian, with English Abstract.) J. Environ. Sci. 5(3): 259-268.
Nasiri, M. 2009. Determination of best method for seed dormancy and germination of Acer monspessulanum, (In Persian with English Abstract)Iranian J. Rangelands Forests Plant Breed. Genet. Res. 16(1);94-105.
Olson David, F. 1974. Robinia L., locust. In Seeds of woody plants in the United States. p. 728-731. C. S. Schopmeyer, tech. coord. U.S. Department of Agriculture, Agriculture Handbook 450. Washington, DC.
883 p.
Ortega Baes Po., L. M de Viana and S.Suhring. 2002. Germination in Prosopis ferox seeds: effects of mechanical, chemical and biological Scarificators. Journal of Arid Environ. 50:185-189.
Pukittayacamee, P. 1991. Seed pretretment. Proceeding of the training course on plantig stock production technology.ASEAN-Canada Forest tee Seed Center Project , Muak – lek ,Saraburi, Thailand.
Sabongari, S. 2001. Effect of soaking duration on germination and seedling establishment of selected varieties of tomato (Lycopersicumesculentum). M.Sc. Thesis, Department of Biological Science.Usmunu Danfodiyo Univ., Sokoto, Nigeria.
Singh, D.p., M.S Hooda and F.T, Bonner. 1991. An evaluation of scarification method for seeds of two legominous trees, New Forests 5: 139-145.
Spaeth J.N. 1934. A Physiological study of dormancy in seed. Cornell Univ. Agric. Experiment Station Memoir, 169: 1-71.
Tavili, A., M Abasi Khaleki, and M. Moameri. 2013. Effect of different treatment on breaking seed dormancy and germination and some charecteries of Astragalus gossypinus, (In Persian with English Abstract)Iranian J. Seed Sci. Technol. 1(1): 64-72.
Thornwaite, C.W. 1931. The climates of North America according to a new classification. Geographical Rev. 21:633-655.
Yousef zadeh, H., M ,Tabari., A, Hossein zadeh karegar., M ,Asadi., A ,Sattarian and H. Zare. 2001. Morphological variety of Tilia spp in Hircanian Forest. (In Persian, with English Abstract.) Taxon. Biosystematics J. 2(3): 11-24.