Haniyeh Saadat; Mohammad Sedghi
Abstract
In order to investigate the effect of hydropriming and hormone priming on some physiological and biochemical characteristics in chickpea under salinity stress a factorial experiment was conducted based on completely randomized design at the University of Mohaghegh Ardabili in 2023 with 3 replications. ...
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In order to investigate the effect of hydropriming and hormone priming on some physiological and biochemical characteristics in chickpea under salinity stress a factorial experiment was conducted based on completely randomized design at the University of Mohaghegh Ardabili in 2023 with 3 replications. treatments included four salinity levels (0, 50, 75 and 100 mM) and four levels of priming (control, hydropriming, priming with gibberellin (50 ppm) and salicylic acid (100 ppm)). The results showed that salinity stress reduced germination percentage (GP), germination rate (GR), radicle and Seedling length (RL and SL) But, seed priming improved these traits. Salinity increased the allometric coefficient (AC), So that the highest was observed at (0.860) salinity of 100 Mm. The highest mean daily germination (MDG), plumule length (PL) and total seed protein content were observed in priming with gibberellin and control (without salinity). The maximum DGS (0.144) and MDA (0.0137 mmol/g FW) were related to the treatment with without priming and salinity of 100 mM. The amount of soluble sugars in gibberellin treatment and salinity of 100 mM compared to the control showed an increase about 56%. Also, the use of gibberellin increased amount of proline by about 40% compared to the control (without priming). In general, seed hydropriming, salicylic acid, especially gibberellin
Haniyeh Saadat; Mohammad Sedghi
Abstract
In order to investigate The effect of priming and aging on the growth indicators and activity of antioxidant enzymes in hybrid maize single cross 704 a factorial experiment was conducted based on a completely randomized design at the University of Mohaghegh Ardabili in 2021 with 3 replications. Treatments ...
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In order to investigate The effect of priming and aging on the growth indicators and activity of antioxidant enzymes in hybrid maize single cross 704 a factorial experiment was conducted based on a completely randomized design at the University of Mohaghegh Ardabili in 2021 with 3 replications. Treatments were aging (control, 7 and 14 days) and priming (control, hydro-priming, priming by gibberellin (20 mg / l) and salicylic acid (100 mg / l)). The results showed that aging decreased growth indicators including Radicle Length (RL), Plumule Length (PL), Seedling Length (SL), Radicle Dry Weight (RDW), Plumule Dry Weight (PDW), Radicle Fresh Weight (RFW), Plumule Fresh Weight (PFW) Seed Length Vigor Index (SLVI) and Seed Weight Vigor Index (SLWI), but types of priming, especially priming with gibberellin improved these traits. As compared to the control, the catalase, superoxide dismutase, and glutathione reductase activities increased respectively by 73%, 63%, and 21% after gibberellin treatment and 14 days of aging. The highest amount of peroxidase enzyme activity (1.797 units mg-1 protein) was related to gibberellin priming. In general, gibberellin pretreatment increased fragile corn seeds' growth indicators and antioxidant enzymes activities, and it can be used to enhance.aging compared to the control showed an increase respectively about 73%, 63% and 21%. The highest amount of peroxidase enzyme activity (1.797 units mg-1 protein) was related to gibberellin priming. In general, using gibberellin pretreatment strengthened weak corn seeds growth indicators and antioxidant enzymes and the treatment can be used to increase weak seed vigor.
Sayyed Saeed Moosavi; Payman Nosrati; Mohammad Reza Abdollahi
Abstract
Datura is an important medicinal plant for production of various alkaloids. Seed dormancy and the problem of germination is the most important barrier to produce this plant. In order to solve the germination problem in this important medicinal plant, the present study was carried out as a factorial experiment ...
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Datura is an important medicinal plant for production of various alkaloids. Seed dormancy and the problem of germination is the most important barrier to produce this plant. In order to solve the germination problem in this important medicinal plant, the present study was carried out as a factorial experiment with three factors based on completely randomized design with three replications. The first factor consisted of two datura ecotypes (Hamadan and Esfahan), the second factor included three levels of physical treatments of breaking dormancy (no scratching; scratch with sandpaper and wet bathing of bain marie bath for 7 days at 60 °C; scratch with sandpaper and dry chilling for 7 days at 4 °C), and the third factor included five levels of chemical treatments of breaking dormancy (no application of chemical treatments [putting the seeds on distil water from the beginning of the experiment to the end of the experiment]; NaOH 20% for 90 minutes; NaOH 20% for 90 minutes + gibberellin 500 ppm for 48 hours; NaOH 20% for 90 minutes + potassium nitrate 300 ppm [putting the seeds in potassium nitrate solution from the beginning of the experiment to the end of the experiment]; NaOH 20% for 90 minutes + gibberellin 500 ppm for 48 hours + potassium nitrate 300 ppm). Interaction of ecotypes and seed dormancy breaking treatments was significant for most traits except; number of leaves per plant, root fresh weight, shoot length and stem fresh weight. The results showed that the chemical treatment of NaOH 20% + gibberellin 500 ppm, was the best dormancy braking treatment for the fresh harvested seeds of datura. Therefore, using this treatment is recommended to break seed dormancy more than 70 percent, without significant damage on the seeds. In general, it was found that the chemical treatments were more effective than physical treatment on breaking the dormancy of datura seed and the main cause of breaking seed dormancy and stimulation of vegetative growth of seedlings obtained was the combination of NaOH with gibberellin.
Tayyebeh Saadat; Mohammad Sedghi; Abdolghayyoum Gholipouri; Raouf Seyed Sharifi; Roghayyeh Sheikhbaglou
Abstract
To examine the effect of priming and deterioration on the activity of antioxidant enzymes, mobility of seed reserves in French bean, a factorial experiment was performed in a completely randomized design with three replications. Treatments included deterioration (control without deterioration, 88% and ...
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To examine the effect of priming and deterioration on the activity of antioxidant enzymes, mobility of seed reserves in French bean, a factorial experiment was performed in a completely randomized design with three replications. Treatments included deterioration (control without deterioration, 88% and 78% of germination of control) and priming (control, hydro-priming, priming by gibberellin and salicylic acid). The results showed that deterioration reduced germination percentage. Priming reduced the effect of deterioration and improved germination percentage. By increasing deterioration, seed reserves use efficiency (SRUE), seed reserves remobilization efficiency (SRRE) and fraction of used seed reserves (FUSR) were reduced, but respiration index (SR) and residual seed dry weight (RSDW) were increased. SRUE reduction was about 30% compared to the control treatment of deterioration and seed respiration index in gibberellin pre-treatment was higher about 24% compared to the control treatment without priming. The total seed protein content in gibberellin pretreatment and without deterioration was increased about 32 percent. The peroxidase activity in gibberellin treatment and deterioration 88% compared to the control showed an increase about 57%. The most superoxide dismutase activity (SOD, 175.2 unit mg-1 protein) was observed in priming with salicylic acid and deterioration 88%. The maximum amount of malondialdehyde (MDA, 1.46 mmol g-1 FW) was related to the treatment with no priming and deterioration 78%. In general, using gibberellin pretreatment strengthened weak bean seeds physiologically and the treatment can be used to increase weak seed vigor.
M. Nemati Khoei; A. Abbasi Surki; S. Fallah
Abstract
Optimizing the best methods to enhancement of seed germination and early seedling growth of Hypericum perforatum L. an experiment was conducted in Shahrekord University in two stages. In the first stage, a factorial experiment was done in a randomized complete block design with four replications in seed ...
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Optimizing the best methods to enhancement of seed germination and early seedling growth of Hypericum perforatum L. an experiment was conducted in Shahrekord University in two stages. In the first stage, a factorial experiment was done in a randomized complete block design with four replications in seed science and Technology lab, Faculty of Agriculture. Treatments consisted osmopriming with PEG in five levels (0, -3, -6, -9 and -12 bar) as first factor and application of GA in four levels (0, 500, 1000 and 1500 ppm) as second factor. results showed that PEG -12 bar + GA 1000 ppm with an average of 91.5% ranked the highest percentage of germination which had no significant difference with PEG -9 bar + GA 1500 ppm and PEG -9 bar + GA 1000 ppm respectively with the germination percentage average 88 and 88.5%, while the control seed with an average (53.5%) had the lowest percentage of germination. As well as combination treatments PEG -9 bar + GA 1000 ppm increased seedling length, seedling dry weight and seed vigor and increased them respectively as by as 1.19, 1.3, 1.96 and 2.14 times than control. The second experiment was conducted in a randomized complete block design with three replications in the research greenhouses conditions, and treatments consisted of the best treatments of first experiment and control. In greenhouse conditions osmopriming PEG -12 bar and application of GA 1000 ppm with an average of 82% ranked the highest percentage of emergence which had better performance than the control with an average of 41.33 %. Finally to enhance seed performance of medicinal plant Hypericum perforatum L., it could be recommended osmopriming with PEG -12 bar and application of GA 1000 ppm or PEG -9 bar + GA 1000 ppm.
