Document Type : Original Article

Authors

1 M.Sc. Graduated of seed Science and Technology of Shahrekord University

2 Faculty member of Shahrekord University, Shahrekord, Iran.

3 Faculty members of Shahrekord University Shahrekord, Iran.

Abstract

Optimizing the best methods to enhancement of seed germination and early seedling growth of Hypericum perforatum L. an experiment was conducted in Shahrekord University in two stages. In the first stage, a factorial experiment was done in a randomized complete block design with four replications in seed science and Technology lab, Faculty of Agriculture. Treatments consisted osmopriming with PEG in five levels (0, -3, -6, -9 and -12 bar) as first factor and application of GA in four levels (0, 500, 1000 and 1500 ppm) as second factor. results showed that PEG -12 bar + GA 1000 ppm with an average of 91.5% ranked the highest percentage of germination which had no significant difference with PEG -9 bar + GA 1500 ppm and PEG -9 bar + GA 1000 ppm respectively with the germination percentage average 88 and 88.5%, while the control seed with an average (53.5%) had the lowest percentage of germination. As well as combination treatments PEG -9 bar + GA 1000 ppm increased seedling length, seedling dry weight and seed vigor and increased them respectively as by as 1.19, 1.3, 1.96 and 2.14 times than control. The second experiment was conducted in a randomized complete block design with three replications in the research greenhouses conditions, and treatments consisted of the best treatments of first experiment and control. In greenhouse conditions osmopriming PEG -12 bar and application of  GA 1000 ppm with an average of 82% ranked the highest percentage of emergence which had better performance than the control with an average of 41.33 %.  Finally to enhance seed performance of medicinal plant Hypericum perforatum L., it could be recommended osmopriming with PEG -12 bar and application of GA 1000 ppm or PEG -9 bar + GA  1000 ppm.

Keywords

Aisvand, H., A. Sharafi and A. Esmaili. 2013. The effect of hydro and osmopriming at different temperatures on germination and seedling growth of Satureja khuzistanica J. under water stress. Ir. J. Medic. Aroma Plant. Res. 29(2): 343-357. (In Persian)
Akers, S.W., and K. E. Holley., 1986. A system for priming seeds using aerated polyethylene glycol or salt solutions. Hort. Sci. 21: 529-531.
Azarnivand, H., M. Abbasi and A. Enayati. 2009. Evaluation and determination of the best hydropriming and osmopriming treatment on germination characteristics of Agropyron elangatum. Ir. J. Nat. Res. 62(4): 431-444. (In Persian)
Cirak, C., K. Kevseroglu., and A.K. Ayan. 2004‌. The Effects of light and some Presoaking treatments on germination rate of Hypericum perforatum L. Seeds. PK. J. Biol. Sci. 7(2): 182-186‌.
Dahal, P., K. I. Bradrord, and R. A. Jones. 1990. Effect of priming and endosperm integrity on seed germination rate of tomato Genotypes. 11. Germination at reduced water potential. J. Exp. Bot. 41: 1441-1453.
Delouche, J. C., and W. P. Coldwell. 1960. Seed vigor and vigor tests. Proc. Assoc. Offic. Seed Anal. 50: 124-129.
Egli, D.B. and D.M. TeKrony. 1995. Soybean seed germination, vigor and field emergence. Seed Sci. Technol. 23, 595-607.
Ghandian Zanjan, M., and D. Eradatmand Asli. 2012. A study of seed germination and early seeding growth of wheat genotypes affected by different seed pyridoxine-priming duration. Ann. Biol. Res. 3(12): 5687-5691.
Gharineh, M. H., A. Bakhshandeh and K. Ghasemi Golozani. 2004. Effect of drought stress and different harvesting stages on seed vigor and germination of wheat cultivars in Ahvaz climatic conditions. J. Agric. Sci. 27(1): 65-76. (In Persian)
Glisic, S., S. Popadic, and D. Skala 2006. St. John’s Wort (Hypericum perforatum L.) -Supercritical extraction, antimicrobial and antidepressant activity of extract and their components. Chem. Ind. J. 60: 61 – 72.
Haigh, A.M., and E. W.R. Barlow. 1987. Germination and priming of tomato, carrot, onion, and sorghum seeds in a range of osmotica. J. Am.  Soc. Hortic. Sci. 112: 202-208.
International Seed Testing Association. 1999. International Rules for Seed Testing. Seed Sci. Techol. 27, Supplement, 333pp.
International Seed Testing Association. 2011. International Rules for Seed Testing. International Seed Testing Association, Bassersdorf, Switzerland.
Jabbari, R., M. Amini-Dehaghi, F. Ganji-Arjenaki., and K. Agahi. 2011. Duration and methods of priming may affect the germination of cumin seeds (Cuminum cyminum L.). J. Agric. 4(4): 23-30. (In Persian, With English Abstract)
Jahani, M., G. Azizi., L. Alimardani and A. Siahmarguei. 2012. Study effective methods on germination and breaking dormancy in some species of medicinal plants. The second Seed Science and Technology Conference. 4 to 5 January. Islamic Azad University of Mashhad. (In Persian)
Joosen R.V.L. Jan Kodde, L.A.J. Willems, Wilco Ligterink. H.W. Linus, and H.W.M. Hilhorst. 2010. GERMINATOR: A software package for high-throughput scoring and curve fitting of Arabidopsis seed germination. Plant J. 62: 148-159.
Abdul-Baki, A. A., and Anderson, J. D. 1973. Vigour determination in soybean by multiple criteria. Crop Sci. 13:630-633.
Karaji Bani, M., 2012. St. Hypericum perforatum L.J. Agric. Anim. Husb. Barzegar. 1069: 18-19. (In Persian)
Makizadeh Tafti, M., R. Farhoodi and M. Rasti far. 2012. The effect of Osmopriming on germination of Melissa officinalis L. Ir. J. Medic. Aroma Plant. Res. 2(4): 573-586. (In Persian, With English Abstract)
Mello, A. M., N. A. Streck., E. E. Blankenship and E. T. Paparozzi. 2009. Gibberellic Acid Promotes Seed Germination in Penstemon digitalis cv. Husker Red. Hortic. Sci. 44(3):870–873.
Mirshekari, B. 2014. The Effect of hormonal and physical treatments on improving the seed germination parameters and seedling vigor of wheat. Ir. J. Seed Sci. Tech. 3(2): 163-171. (In Persian)
Musavi, S. R., J. Ahmadi and F. Sefidkan. 2012. Optimization of seed breaking dormancy to increase seed germination Hypericum perforatum L. The Twelfth Congress of Agronomy and plant breeding in Iran. 14 to 16 September. Islamic Azad Univ. Karaj. (In Persian)
Nadjafi, F., M. Bannayan., L. Tabrizi and M. Rastgoo. 2006. Seed germination and dormancy breaking techniques for Ferula gummosa and Teucrium polium. J. Arid Environ. 64: 542-547.
Neamatollahi, E., M. Bannayan, A. Ghanbari, M. Haydari and A. Ahmadian. 2009. Does hydro and osmo- priming improve fennel (Foeniculum vulgare) seeds germination and seedlings growth? Not. Bot. Hort. Agrobot. Cluj 37 (2): 190-194.
Radusienea, J., A. Judzentieneb and G. Bernotieneb, 2005. Essential oil composition and variability of Hypericum perforatum L. growing in Lithuania. Biochem Syst Ecol. 55: 113 – 24.
Rahmanpur, A., A. Majda and F. Chalbian. 2007. The effect of hormonal and mechanical treatments on breaking dormancy of seeds of medicinal plants Eremurus olgae Regel. Ir. J. Medic. Aroma Plant. Res. 23: 111-120. (In Persian, With English Abstract)
Riyazi, A., F. Sharifzadeh, and A.Ahmadi, 2007. Effects of osmopriming on seeds germination of forage millet. Res. Dev. Agric. Hortic. 77: 72-83. (In Persian, With English Abstract)
Roohi, A., M. Tajbakhsh., E. Barnosi., M. Saeedi., and P. Nikzad. 2011. Investigation of different pre-treatments effects on seed germination and seedling traits of various chickpea (Cicer arietinum L.) cultivars. Agron. J. (Pajouhesh & Sazandegi). 90: 1-8. (In Persian, With English Abstract)
Sajadi, S. S., D. Eradatmand Asli., and H. Ardalani. 2013. The effect kinetin on seed germination parameters Hypericum perforatum L. National Conference of applied research in science and engineering. 24-26 April. Takestan Islamic Azad Univ. (In Persian)
Shafe, M., M. Behdani and M. Jami Al Ahmad. 2013. The effect of Osmopriming on germination and seedling establishment of Hyoscyamus niger L. Ir. J. Medic. Aroma Plant. Res. 28(4): 646-656. (In Persian, With English Abstract)
Shahsavand, K., R. Tavakol Afshar and M. chaiahi. 2009. The effect of Osmopriming on germination characteristics of four plant species seed under drought stress. J. Range. 3(1): 479-490. (In Persian)
Taiz, L., and E. Zieger. 2006. Plant Physiology. 3rd end.  Eng. Sunderland. Sinauer Assoc.