The effect of priming and deterioration on the activity of antioxidant enzymes and the mobility of seed reserves in French bean (Phaseolus vulgaris L.) cv. Sadri

Document Type : Original Article

Authors

1 University of Mohaghegh Ardabili, Faculty of Agriculture and Natural Resources, Department of Agronomy and Plant Breeding

2 Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili

Abstract

To examine the effect of priming and deterioration on the activity of antioxidant enzymes, mobility of seed reserves in French bean, a factorial experiment was performed in a completely randomized design with three replications. Treatments included deterioration (control without deterioration, 88% and 78% of germination of control) and priming (control, hydro-priming, priming by gibberellin and salicylic acid). The results showed that deterioration reduced germination percentage. Priming reduced the effect of deterioration and improved germination percentage. By increasing deterioration, seed reserves use efficiency (SRUE), seed reserves remobilization efficiency (SRRE) and fraction of used seed reserves (FUSR) were reduced, but respiration index (SR) and residual seed dry weight (RSDW) were increased. SRUE reduction was about 30% compared to the control treatment of deterioration and seed respiration index in gibberellin pre-treatment was higher about 24% compared to the control treatment without priming. The total seed protein content in gibberellin pretreatment and without deterioration was increased about 32 percent. The peroxidase activity in gibberellin treatment and deterioration 88% compared to the control showed an increase about 57%. The most superoxide dismutase activity (SOD, 175.2 unit mg-1 protein) was observed in priming with salicylic acid and deterioration 88%. The maximum amount of malondialdehyde (MDA, 1.46 mmol g-1 FW) was related to the treatment with no priming and deterioration 78%. In general, using gibberellin pretreatment strengthened weak bean seeds physiologically and the treatment can be used to increase weak seed vigor.

Keywords

References

Aebi, H. 1984. Catalase in vitro. Methods Enzymol. 105: 121-126.
Alizadeh, A. 1997. Water, Soil and Plants Relations. Astan Quds Razavi Press.
Anonymous. 2008. Food Outlook, Global Market Analysis [Online]. Available at http://www.fao.food outlook.com
Ansari, O., R. Tavakkol Afshari, F. Sharif-Zadeh, and A. Shayanfar. 2013. The role of priming on seed reserve utilization and germination of mountan rye (Secale montanum) seeds under salinity stress. (In Persian, with English Abstract.) Iranian J. Field Crop Sci.44(2): 181-189.
Ashraf, M., H. R. Athar, P. J. C. Harris, and T. R. Kwon. 2008. Some prospective strategies for improving crop salt tolerance. Adv. Agron. 97: 45-92.
Bailly, C. 2004. Active oxygen species and antioxidants in seed biology. Seed Sci. Res. 14: 93- 107.
Bailly, C., A. Benamer, F. Cornineau, and D. Come. 2000. Antioxidant systems in sunflower (Helianthus annuus L.) seeds as affected by priming. Seed Sci. Res. 10: 35-42.
Berlett, B. S., and E. R. Stadtman. 1997. Protein oxidation in aging, disease, and oxidative stress. J. Biol. Chem. 272: 20313-20316.
Bray, C. M., P. A. Davision, M. Ashraf, and R. M. Taylor. 1989. Biochemical changes during osmopriming of leek seed. Ann. Bot. 63: 185-193.
Bruggink, G.T., J. J. J. Ooms, and P. Van der Toorn, 1999. Induction of longevity in primed seeds. Seed Sci Res. 9: 49- 53.
Burguieres, E., P. McCu, Y. Kwon, and K. Shetty. 2007. Effect of vitamin C and folic acid on seed vigour response and phenolic-linked antioxidation activity. Bioresour. Technol.98: 1393-1404.
Chen, K., and R. Arora. 2013. Priming memory invokes seed stress-tolerance. Environ. Exp. Bot. 94: 33-45.
Cruz de Carvalho, M. H. 2008. Drought stress and reactive oxygen species. Plant Signal Behav. 3: 156-165.
Davison, P. A., R. M. Taylor, and C. M. Bray. 1991. Changes in ribosomal RNA integrity in leek (Allium porrum L.) seeds during osmopriming and drying-back treatments. Seed Sci. Res. 1(1): 37-44.
Duman, I. 2006. Effect of seed priming with PEG and K3PO4 on germination and seedling growth in lettuce. Pakistan J. Biol. Sci.9(5): 923-928.
Ellias, S. G., A. L. Garary, and S. Haning. 2006. Seed quality testing of native species.Native Plant J. 7: 15-19.
Ellis, R. H., and E. H. Roberts. 1980. Towards a rational basis for testing seed quality. p. 605-635. In P.D. Hebblethwaite (ed.). Seed Production. Butterworth’s, London.
El-Shintinawy, F., and M. N. El-Shourbagy, 2001. Alleviation of changes in protein metabolism in NaCl -stressed wheat seedlings by thiamine. Biol. Plant. 44: 541-545.
El-Tayeb, M. A. 2005. Response of barley gains to the interactive effect of salinity and salicylic acid. Plant Growth Regul. 45: 215-225.
Espin, G. B., P. D. Vivancos, D. Job, M. Belghazi, C. Job, and J. A. Hernandez. 2011. Understanding the role of H2O2 during pea seed germination: a combined proteomic and hormone profiling approach. Plant Cell Environ. 34: 107-1919.
Farooq, M., S. M. A. Basra, E. A. Warraich, and A. Khaliq, 2006. Optimization of hydropriming techniques for rice seed invigoration. Seed Sci. Technol. 34: 529-534.
Giannopolitis, C. N., and S. K. Ries, 1977. Suoeroxide dismutase. I. Occurrence in higher plants. J. Plant Physiol. 59: 309-314.
Hampton, J. G. 2003. Methods of viability and vigour testing: a critical and appraisal. p. 81-118. In A. S. Basra (ed.) Seed Quality: Basic Mechanisms and Agricultural Implications, CBS Publishers and Distributers, New Delhi, India.
Harris, D., J. K. R. Kumar, and J. Kumar. 2001. On- farm seed priming. Agric. Res. 44: 3-14.
Kaewnaree, P., S. Vichitphan, P. Klanrit, B. Siri, and k. Vichitphan, 2011. Effect of accelerated Aging Process on Seed Quality and Biochemical Chnages in Sweet Pepper (Capsicum annuum L.). Seed Biotechnol. 10(2): 175- 182.
Kalpana, R., and M. K. V. Rao, 1995. On the ageing mechanismin pigeon pea (Cajanus cajan (L.) Mill sp.) seeds. Seed Sci. Technol. 23: 1-9.
Kant, S., S. Pahuja, and R. K. Pannu. 2006. Effect of seed priming on growth and phenology of wheat under late-sown conditions. Tropical Sci. 44: 9-15.
Kaur, S., A. K. Gupta, and N. Kaur. 2005. Seed priming increase crop yield possibly by modulating enzymes of sucrose metabolism in chickpea. J. Agron. Crop Sci. 191: 81-87.
KrishnaChaitanya, K. S., S. Keshavkant, and S. C. Naithani. 2000. Changes in total protease activity in dehydrating recalcitrant sal (Shorea robusta) seeds. Silva Fen. 34: 71-77.
Lee, S. S., and J. H. Kim. 2000. Morphological change, sugar content, α-amylase activity of rice seed various priming conditions. Korean J. Crop Sci. 44: 138-142.
Leprince, O., N. M. Atherton, R. Deltour, and G. A. F. Hendry. 1994. The involvement of respiration in free radical processes during loss of desiccation tolerance in germination Zea mays L. An electron paramagnetic resonance study. Plant Physiol. 104: 1333-1339.
MacAdam, J. W., R. Nelson, and E. Sharp. 1992. Peroxidase activity in the leaf elongation zone of tall fescue. Plant Physiol. 99: 872-878.
McCue, P., and K. Shetty. 2002. A biochemical analysis of mungbean (Vignar adiata) response tomicrobial polysaccharides and potential phenolicenhancing effects for nutraceuticalappli-cations. Food Bio. 16: 57- 79.
MacDonald, M. B. 1999. Seed deterioration: physiology, repaired and assessment. Seed Sci. Technol. 27: 177-237.
McDonald, M.B. 2000. Seed priming. p. 287-325.  In M. Black and J. D Bewley (ed) Seed technology and its biological basis. Sheffield Academic Press, UK.
Magbanua, Z. V., C. M. D. Moraes, T. D. Brooks, W. P. Williams, and D. S. Luthe. 2007. Is Catalase Activity One of the Factors Associated with Maize Resistance to Aspergillus flavus. Mol. Plant-Microbe Interact. 20(6): 697–706.
Mauch, F., B. Mauch-Mani, C. Gaille, B. Kull, D. Haas, and C. Reimmann. 2001. Manipulation of salicylate content in Arabidopsis thaliana by the expression of an engineered bacterial salicylate synthase. J. Plant. 25(1): 67–77.
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7: 405-410.
Mittler, R., S. Vanderauwera, M. Gollery, and F. Van Breusegem. 2004. Reactive oxygen gene network of plants. Trends Plant Sci. 9: 490-498.
Mohammadi, M., H. Fahimi, and A. Mogar. 2009. Effects of salicylic acid and gibberellic acid on seed germination rate in lentils. (In Persian, with English Abstract.) J. Bio 4:33-44.
Mohanty, N. 2003. Photosynthetic characteristics and enzymatic antioxidant capacity of flag leaf and the grain yield in two cultivars of Triticum aestivum (L). exposed to warmer growth conditions. J. Plant Physiol. 160:71-74.
Moosavi, A., R. Tavakkol-Afshari, F. Sharif-Zadeh, and A. Aynehband. 2009. Effect of seed priming on germination characteristics, polyphenol oxidase, and peroxidase activities of four amaranth cultivars. J. Food Agri. Environ. 7: 353-358.
Patil, M. N. 2010. Biofertilizer effect on growth protein and carbohydrate content in stevia rebaudianavar bertoni. Sci. Technol. 2(10): 42-44.
Ray, M. B., S. Halder, and K. Gupta, 1990. Differential responses of early and late cultivars of rice seeds under accelerated ageing. Seed Sci. Technol. 18: 823-831.
Rouzrokh, M., and K. Ghasemi Golezani. 1998. The effect of aging on seed emergence and yield and yield components of two chickpea cultivars under full irrigation and deficit irrigation. M. Sc. Thesis. Univ. of Tabriz, Iran.
Sairam, R. K., K. V. Rao, and G. C. Srivastava. 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyteconcentration. Plant Sci. 163: 1037-1046.
Savage, W. E. F., K. C. Dent, and L. J. Clark. 2004. Soak condition and temperature following sowing influence the response of maize (Zea Mays L.). Field Crops Res. 90: 361-374.
Sedghi, M., S. Khomari, and B. Amanpour-Balaneji. 2011. Effect of seed vigor and hormone priming on glyoxylate cycle enzymes activity in Persian silk tree (Albiziajulibrissin Durazz.). World App. Sci. J. 13(3): 541-544.
Sedghi, M., A. Nemati, B. Amanpour-Balaneji, and A. Gholipouri. 2010a. Influence of different priming materials on germination and seedling establishment of milk thistle (Silybum marianum) under salinity stress. Word App. Sci. J. 11(5): 604-609.
Sedghi, M., A. Nemati, and B. Esmaielpour. 2010. Effect of seed priming on germination and seedling growth of two medicinal plants under salinity. Emir. J. Food Agric. 22 (2): 130-139.
Slaymarker, D. H., D. A. Navarre, D. Clark, O. D. Pozo, G. B. Martin, and D. F. Klessig. 2002. The Tobacco salicylic acid-banding protein 3 (SABP3) is the chloroplast carbonic anhydras, which exhibition antioxidant activity and plays a role in the hypersensitive defense response. PNAS. 99 (18):11640-11645.
Soltani, E., B. Kamkar, S. Galeshi, and F. Akram Ghaderi. 2008. The effect of seed deterioration on seed reserves depletion and heterotrophic seedling growth of wheat. (In Persian, with English Abstract.) J. Agric. Sci. Natur Resorce 15(1):13-17.
Van Schoonhoven, A., and O. Voysest (eds.) 1991. Common Beans: Research for Crop Improvement. Wallingford: CAB International, in association with CIAT. pp. 980.
Varier, A., A. Kuriakose, and M. Dadlani. 2010. The subcellular basis of seed priming. Current Sci. 99(4): 450-456.
Vitoria, A. P., P. J. Lea, and R. A. Azevedo. 2001. Antioxidant enzymes responses to cadmium in radish tissues. Phytochem. 57: 701-710.
Wahid, A., A. Noreen, S. M. A. Basra, S. Gelani, and M. Farooq. 2008. Priming-induced metabolic changes sunflower (Helianthus annuus) achenes improve germination and seedling growth. Bot. stud. 49: 343-350.
Wang, F., R. Wang, W. Jing, and W. Zhang. 2012. Quantitative dissection of lipid degradation in rice seeds during accelerated aging. Plant Growth Regul. 66: 49-58.
Iranian Journal of Seed Science and Technology
Volume 8, Issue 2 - Serial Number 16
December 2019
Pages 19-32

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