Effect of seed priming and cold treatment to improve seed germination characteristics and vigor of three population of Sahandi medicinal herb of savory (Satureja sahendica

Document Type : Original Article

Abstract

In order to evaluate the priming effects of gibberllic acid and potassium nitrate on germination and seedling growth characteristics of three populations Satureja sahendica،, an experiment was conducted. The treatments including plant populations (Majarition, Zeninjab and Osko), gibberllic acid (500, 250 ppm) and potasium nitrate (1%, 2%) and cold treatment in three replications arranged in factorial was carried out in greenhouse and laboratory conditions in Natural  Gene Bank  Group of  Research Institute of Rangeland and Forestry in 2011- 2012. After seedling growth, traits including: percentage and speed of germination, root and shoot length ,  ratio of root length by shoot length, vigor index, normal seedlings, seedling length, fresh and dry weight and ratio of dry weight to fresh weight were measured in laboratory condition. Also in greenhouse, traits including: percentage and speed of emergence, root and shoot length ,  ratio of root length by shoot length, normal seedlings, seedling length, fresh and dry weight and ratio of dry weight to fresh weight were measured. Analysis of variance showed that there were significanc differences between populations,  treatments and interaction of populations with treatments( 5% ) for all traits  in both experimental conditions except  for seed emergence percentage and shoot length in greenhouse condition. Comparing seed germination characteristics of three population showed that population of Mjarishen  selected from East Azarbijan had higher vigor characteristics like seed germination (97%), speed of germination (15 sprout/day) and vigor index(41) than other populations in laboratory condition. In greenhouse condition, percentage and speed of emergence of Osko population as 17%  and  1 sprout/day, respectively, were higher than other two populations.  Effect of  treatment on the populations showed  that priming of the seeds with gibberllic acid were more effective for vigor traits like(germination speed, emergence speed and vigor index)  than the other treatments in both experimental conditions.

Keywords


Abdul-baki, A.A., Anderson, J.D., 1975. Vigour determination in soybean seed by multiple criteria. Crop
Sci. 13:630-633.
Afzal, L., Basra, M. A., Faroog, SH. M., Nawaz, A. 2006. Alleviation of salinity stress in spring wheat by
hormonal priming with ABA, salicylic acid and ascorbic acid . Int . J. Agric . & Biol. 1:23-28
Akram Ghaderi, F. Kamkar, B. and Soltani, A. 1997. Seed technology Science, Mashhad university
jehad, first press P 512.
Aliarab, H., Alizadeh, M.A.. Nasiri, M and and Tabaii, R. 2012. The study of seed and seedling
enhancement of germination and vigour some population of Satureja mutica with overcoming of dormancy
by using of physical and physiological tratment 12th Agronomy and plant breeding Congress, Iran , Karaj
Islamic Azad university 4-6 Sep , 2012.
Aliarab, H., Alizadeh, M.A.. Nasiri, M and and Tabaii, R. 2012. Evaluation of seed and seedling
enhancement of germination and vigour potential of some population of Satureja bachtiarica with physical
and chemical treatment . 12th Agronomy and plant breeding Congress, Iran , Karaj Islamic Azad university
4-6 Sep , 2012.
Alizadeh, M.A. Aliarab, H. Nasiri,M and Jafari A.A 2012. Evaluation of germination and seedling vigor
of some populations Satureja sahendica, the use of physical and chemical treatments. 12th Agronomy and
plant breeding Congress, Iran , Karaj Islamic Azad university 4-6 Sep , 2012.
Armin, M., Asgharipour, M ., Razavi – Omrani, M. 2010. The Effect of Seed Priming on Germination and
Seedling Growth of Watermelon (Citrullus Lanatus) Advances in Environ Biol . 4 (3) : 501 – 505.
Ashraf, M., Foolad, M. R. 2005. Presowing seed treatment, a shotgun approach to improve germination ,
plant growth and crop Yield under saline and non-saline conditions . Advances in Agronomy . 88 : 223-27.
Casenave, E. C., Toselli, M.E. 2007. Hydropriming as a Pre-treatment for cotton germination under thermal
and water stress conditions . Seed Sci & Technol. 35 : 88-98.
Ehiaei, H.R. and Khageh Hoseini,M 2011. Evaluation of medicinal plant seed germination and dormancy
in thirty seed population , Journal of Agricultural Research of Iran. Vol 9(4): PP: 651-658.
Elkoca, E., Haliloglo, K., Esitken, A. and Ercisli, S. 2007. Hydro- and osmopriming improve chickpea
germination . Soil and Plant Science. 57: 193-200.
Faker Baher, Z., Rezaei, M.B., Mirza, M and Abbaszadeh, B. 2001. Quantitative and qualitative study of
oils during drought stress , Medicinal and Aromatic Plant, Volume (11), pp: 12-22.
Farooq, M., S.M.A. Basra., K. Hafeez., S.A. Asad. and N. Ahmad. 2005. Use of Commercial fertilizers as
osmotic for rice priming. J. Agr. Sci. 12:172- 175
Ghassemi-Golezani, K., Sheikhzadeh-Mosaddegh, P. Valizadeh, M., 2008. Effects of hydropriming
duration and limited irrigation on field Performance of chickpea. Res. J .Seed Sci,1(1) :34-40.
Ghayyad, M., Kurbysa, M., Napolsy, Gh. 2010. Effect of Endocarp Removal, Gibberelline, Stratification
and Sulfuric Acid on Germination of Mahaleb (Prunus mahaleb L.) Seeds. American-Eurasian J. Agric. &
Environ. Sci., 9 (2): 163-168.
Ghiyasi, M., Pouryousef Miyandoab, M., Tajbakhsh, M. 2008. In fluence of osmopriming treatment on
emerengency and Yield of maize (Zea mays L .) Research . J . Biol .Sci .3 (12): 1452 – 1455.
Girolamo, G, D., Barbanti, L. 2012. Treatment conditions and biochemical processes.
Harris, D., Raghuwenshi, B. S., Gangwar, J. S., Singh, S. C., Joshi, K. B., Rashid, A., Hollington, P. A.
2001. Participatory evaluation by farmers of on-farm seed priming in wheat in India Nepal and Pakistan.
Exp. Agric . 37 : 403-415.
Hosseini, A., Koocheki, A. 2007. The effect of different priming treatments on germination percent and
mean germination time of four varieties of sugar beet . J. Agron . Res. 5 (1) : 69-76.
influencing seed priming effectiveness. Italian. J. Agro. V 7: e 25.
Jamzad, Z., 2009. Thymes and savory of Iran, part two, research Institute range land and forestry, Tehran,
Pp 119.
 
Kaur, S., Gupta, A., kuar, N. 2004. Gibberellin A3 chickpea (Cicer arietinum L.) seedlings by enharfing
amylase activity and mobilization of starch cotyledons .Plant Growth Regul . 26: 85-90.
Kaya, M. D., Okcu, G., Atak, M., Kolsarici, Y. 2006. Seed treatments to overcome salt and drought stress
during germination in sunflower (Helianthus annus L.). Eur. J. Agronomy. 24: 291-295.
Maguire, J. D. 1962 . Speed of germination in selection and evaluation for seedling vigour. Crop Sci . 2 :
176-177.
Mehra, R., Raaj, R. 2002. Mood fluctuations, Projection bias, and volatility of equity Prices. J. Economic.
Dynamics and Control, 26 (5): 869-887.
Pill, w.G., Necker, A. D. 2001. The effects of seed treatments on germination and stablishment of Kentucky
bluegrass (Poa pratensis L.) Seed Sci .Technol. 29: 65-72.
Sarmadniya, Gh., 1996. Seed tecnolegy Science. Mashhad university jehad, first press. P267.
Shafiei, M. 2012.E ffect of temperature and drought stress on germination and seedling growth of
populations from three species of Satureja sahendica ، S.bachtiarica and S.khuzistanica species in
laboratory and greenhouse .M.Sc. Thesis. Islamic Azad University Boroujerd Branch. Tvakol Afsharyi, R.,
Badry S. and . Abbasi, A. 2010. Effects of gibberellin and abscisic acid on germination, induction of acid
and alkaline phosphatase enzyme activity Rkvdv seed embryos of wheat (Triticum aestivum) cultivars
RL4137. Iranian Journal of Crop Science. Vol 41 (4). PP: 781-789.
Zargary, A., 1997. Plant Medicin. 6th Edn., Theran University Press, Tehran, Iran. . (In Persian)