Accelerated agingeffect on lipid peroxidation and antioxidant enzymes activity of two soybean cultivars

Document Type : Original Article

Abstract

In order to identify the accelerated aging effects on enzyme and lipid peroxidation in soybean an experiment was conducted based on completely randomized design by factorial arrangement with five levels of seed aging (3, 6, 9, 12, days to aging) and without aging, and two cultivars (Katol and Sahar) with three replications in seed testing Lab of Agricultural and Natural Research Center of Golestan. Accelerated aging was achieved by incubating the seed in a closed plastic box at 40 degree centigrade and close to 100% relative humidity for up to 12 days. Seed viability, electrical conductivity, lipid peroxidation, superoxidedismutase, catalase, peroxidase, polyphenol oxidase, Ascorbate peroxidase activity were measured in aged and non-aged seed. The result indicated that antioxidant enzyme activities except peroxidase were reduced and it causedhigher malondialdehyde (MDA) and electrolyte leakage (EC). All of this effect caused lower seed vigor during aging. There was significant correlation between EC with percentage of seed germination and showed EC could be considered as a rapid method for evaluation of aged seed. Results showed that Katol cultivar was more tolerant than Sahar cultivar to seed aging.

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References

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