Document Type : Original Article

Abstract

In order to identify the accelerated aging effects on enzyme and lipid peroxidation in soybean an experiment was conducted based on completely randomized design by factorial arrangement with five levels of seed aging (3, 6, 9, 12, days to aging) and without aging, and two cultivars (Katol and Sahar) with three replications in seed testing Lab of Agricultural and Natural Research Center of Golestan. Accelerated aging was achieved by incubating the seed in a closed plastic box at 40 degree centigrade and close to 100% relative humidity for up to 12 days. Seed viability, electrical conductivity, lipid peroxidation, superoxidedismutase, catalase, peroxidase, polyphenol oxidase, Ascorbate peroxidase activity were measured in aged and non-aged seed. The result indicated that antioxidant enzyme activities except peroxidase were reduced and it causedhigher malondialdehyde (MDA) and electrolyte leakage (EC). All of this effect caused lower seed vigor during aging. There was significant correlation between EC with percentage of seed germination and showed EC could be considered as a rapid method for evaluation of aged seed. Results showed that Katol cultivar was more tolerant than Sahar cultivar to seed aging.

Keywords

Alscher, R.G., N. Erturk, and Heath, L.S. 2002. Role of superoxide dismutases (SODs) in controlling
oxidative stress in plants. J. Exp. Bot. 53: 1331-1341.
  Arora, A., R. Sairam, Srivastava, G. 2002. Oxidative stress and antioxidative system in plants. Current
Science 82: 1227-1238. Association, I.S.T., 2010. The germination test. International Rules for Seed Testing. Bassersdorf:
International Seed Testing Association, 1-46. Bailly, C., 2004. Active oxygen species and antioxidants in seed biology. Seed Sci. Res. 14:93-107. Bailly, C., A. Benamar, F. Corbineau, CÙme, D. 2000. Antioxidant systems in sunflower (Helianthus
annuus L.) seeds as affected by priming. Seed Sci. Res. 10: 35-42. Basra, S., N. Ahmad, M. Khan, N. Iqbal, M. Cheema, 2003. Assessment of cottonseed deterioration
during accelerated ageing. Seed Sci. and Technol. 31: 531-540. Beauchamp, C.O., S.L. Gonias, D.P. Menapace, S.V. Pizzo, 1983. A new procedure for the synthesis of
polyethylene glycol-protein adducts; effects on function, receptor recognition, and clearance of superoxide
dismutase, lactoferrin, and alpha 2-macroglobulin. Anal. Biochem. 131: 25-33. Cho, U. H., Seo, N. H. 2005. Oxidative stress in (Arabidopsis thaliana) exposed to cadmium is due to
hydrogen peroxide accumulation. Plant Sci. 168: 113-120. Goel, A., A.K. Goel, I.S. Sheoran, 2003. Changes in oxidative stress enzymes during artificial ageing in
cotton (Gossypium hirsutum L.) seeds. J. Plant Physiol. 160: 1093-1100. Goel, A., and Sheoran, I. 2003. Lipid peroxidation and peroxide-scavenging enzymes in cotton seeds under
natural ageing. Biol. plantarum 46: 429-434. Heath, R.L., Packer, L. 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of
fatty acid peroxidation. Arch. Biochem. Biophys. 125: 189-198. Kar, M., D. Mishra, 1976. Catalase, peroxidase, and polyphenoloxidase activities during rice leaf
senescence. Plant Physiol. 57: 315-319. Kocsy, G., R. Laurie, G. Szalai, V. Szilagyi, L. Simon‐Sarkadi, G. Galiba, De Ronde, J.A. 2005. Genetic
manipulation of proline levels affects antioxidants in soybean subjected to simultaneous drought and heat
stresses. Physiol. Plantarum 124: 227-235. Koroi, S., 1989. Gel electrophoresis tissue and spectrophotometrscho unter uchungen zomeinfiuss der
temperature auf struktur der amylase and peroxidase isoenzyme'. Physiol. Rev. 20: 15-23. Lehner, A., N. Mamadou, P. Poels, D. CÙme, Bailly, C. Corbineau, F. 2008. Changes in soluble
carbohydrates, lipid peroxidation and antioxidant enzyme activities in the embryo during ageing in wheat
grains. J. Cereal Sci. 47: 555-565. Maehly, A., Chance, B. 1954. Catalases and peroxidases. Methods of biochemical analysis 1: 357-424. McDonald, M., 1999. Seed deterioration: physiology, repair and assessment. Seed Sci. & Technol. 27: 177- 237. Murthy, U.N., P.P. Kumar, Sun, W.Q. 2003. Mechanisms of seed ageing under different storage conditions
for Vigna radiata (L.) Wilczek: lipid peroxidation, sugar hydrolysis, Maillard reactions and their relationship
to glass state transition. J. Exp. Bot. 54: 1057-1067. Nakano, Y., Asada, K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach
chloroplasts. Plant and Cell Physiol. 22: 867-880. Noctor, G., Foyer, C.H. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annu. Rev. Plant Biol. 49: 249-279. Priestley, D.A., 1986. Seed aging: implications for seed storage and persistence in the soil. Comstock
Associates. Simontacchi, M., A. Caro, C.G. Fraga, Puntarulo, S. 1993.
Oxidative stress affects [alpha]-tocopherol
content in soybean embryonic axes upon imbibition and following germination. Plant physiol. 103: 949-953. Stewart, R.R., Bewley, J.D. 1980.
Lipid peroxidation associated with accelerated aging of soybean axes. Plant physiol. 65: 245-248. Sung, J., 1996. Lipid peroxidation and peroxide-scavenging in soybean seeds during ageing. Plant Physiol. 97: 85ñ89. Sung, J., T. Jeng, 2006. Lipid peroxidation and peroxide‐scavenging enzymes associated with accelerated
aging of peanut seed. Physiol. Plantarum 91: 51-55. Verma, S., U. Verma, Tomer, R. 2003. Studies on seed quality parameters in deteriorating seeds in Brassica
(Brassica campestris). Seed Sci. Technol. 31: 389-396.