The effect of accelerated aging on seed germination traits produced from treated mother plant in three bean cultivars

Document Type : Original Article

Authors

1 PhD Student, Department of Agronomy, Faculty of Agriculture, Zabol University, Iran

2 Associate Professor, Department of Agronomy, Faculty of Agriculture, Zabol University, Iran.

3 Associate Professor, Department of Agronomy, Faculty of Agriculture, Shiraz University, Iran.

Abstract

Seed senescence is one of the major problems in the production of crops. In order to evaluate the effect of accelerated aging on germination and other seed germination components produced from the treated mother plant in three bean cultivars, a factorial experiment was conducted in a randomized complete block design with three replications at the research laboratory of Faculty of Agriculture, Shiraz University. The factors were including pinto bean varieties at 3 levels includes E9, E10, and Khomain, accelerated aging at two levels includes accelerated aging and control, and the third factor was seeds produced from treated mother plant seed in the farm at 7 levels includes: polyethylene glycol (PEG) -5 bar at 6 hours, , potassium chloride (KCl) -20 mM at 6 hours, calcium chloride (CaCl2) - 15 mM at 3 hours, sodium chloride (NaCl) -20 mM at 6 hours, salicylic acid (C7H6O3) 500 mg per liter at 12 hours, distilled water at 12 hours and without pretreatment (Control). The percentage and rate of germination, seedling dry weight, weight and length vigor index and 9th day germination energy were measured. The result showed that the interaction between accelerated aging, pretreatment and variety on germination traits including germination percentage, seedling dry weight, weight and length vigor index and germination energy of the 9th day at 1 % level, interaction between accelerated aging and pretreatment on germination rate at 1 % level and interaction between pretreatment and variety on germination rate and coefficients of allometric at 5 % level were significant. Seed germination traits at accelerated ageing treatment was lower than control. The maximum germination percentage and germination energy of the 9th day was for sodium chloride at 6 hours and E10 variety, maximum seedling dry weight, germination rate and weight and length vigor index in calcium chloride (CaCl2) at 3 hours treatment and E10variety were detected. The result showed that according to the variety and selection of appropriate pretreatment of mother plant, it is possible to improve storage and have seeds with higher power and less deterioration.
Seed senescence is one of the major problems in the production of crops. In order to evaluate the effect of accelerated aging on germination and other seed germination components produced from the treated mother plant in three bean cultivars, a factorial experiment was conducted in a randomized complete block design with three replications at the research laboratory of Faculty of Agriculture, Shiraz University. The factors were including pinto bean varieties at 3 levels includes E9, E10, and Khomain, accelerated aging at two levels includes accelerated aging and control, and the third factor was seeds produced from treated mother plant seed in the farm at 7 levels includes: polyethylene glycol (PEG) -5 bar at 6 hours, , potassium chloride (KCl) -20 mM at 6 hours, calcium chloride (CaCl2) - 15 mM at 3 hours, sodium chloride (NaCl) -20 mM at 6 hours, salicylic acid (C7H6O3) 500 mg per liter at 12 hours, distilled water at 12 hours and without pretreatment (Control). The percentage and rate of germination, seedling dry weight, weight and length vigor index and 9th day germination energy were measured. The result showed that the interaction between accelerated aging, pretreatment and variety on germination traits including germination percentage, seedling dry weight, weight and length vigor index and germination energy of the 9th day at 1 % level, interaction between accelerated aging and pretreatment on germination rate at 1 % level and interaction between pretreatment and variety on germination rate and coefficients of allometric at 5 % level were significant. Seed germination traits at accelerated ageing treatment was lower than control. The maximum germination percentage and germination energy of the 9th day was for sodium chloride at 6 hours and E10 variety, maximum seedling dry weight, germination rate and weight and length vigor index in calcium chloride (CaCl2) at 3 hours treatment and E10variety were detected. The result showed that according to the variety and selection of appropriate pretreatment of mother plant, it is possible to improve storage and have seeds with higher power and less deterioration.

Keywords


Abdul – Baki, A.A., and J.D. Anderson. 1973.Vigor determination in soybean by multiple criteria. Crop Sci. 13: 630-633.
Agarwal, R. L. 2003. Seed technology. Publication Company Limited New Delhi, India. 550pp.
Basra, S.M.A., N. Ahmad, M.M. Khan, N. Iqbal, and M.A Cheema. 2003. Assessment of cotton seed deterioration during accelerate. Seed. Sci. Technol. 31: 531-540.
Bayat, M., and B. Rabiee. 2006. The effect of cold stress and aging on the components germination and seedling growth of five Rapeseed, (In Persian with English Abstract). J. Agric. Sci. Nat. Res .2(7): 46-57
Copeland, L.O., and M.B. Mcdonald. 1985. The Chemistry of seeds. In Principles of seed science and technology, 2nd Ed., Macmillan Publ. Company, Macmillan. INS, New York.
Dehghan Shoar, M., A. Hamidi, and S. Mobaser. 2005. Handbook of Vigor Test Methods (3rd ed). (In Persian). Publ. Agric.Educ. 193p.
Demir Kaya, M., G. Okçu, M. Atak, Y. Çikili, and Ö. Kolsarici. 2006. Seed treatment to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Eur. J. Agron., 24: 291-295.
Farooq, M., A., Wahida, N., Ahmadand Asad, S. 2010. Comparative efficacy surface drying and re-drying seed priming in rice. Change in emergence, seedling growth and associated metabolic events. Paddy Water Environ. 8: 15-22.
Ghasemi-Golezani K., P. Sheikhzadeh-Mosaddegh, and M. Valizadeh. 2008. Effects of hydro-priming duration and limited irrigation on field performance of chickpea. Res. J. Seed. Sci., 1(1): 34-40.
Hamidi, A., D. Dori, V. Askari, and S. Hajiloee. 2009. Evaluation of applicability controlled fatigue test to assess the relationship between seed vigor and growth of three varieties of canola field. Seed. Plant Prod. J. 24(4): 677-705(In Persian).
Hampton, J.G. 2003. Methods of Viability and Vigour Testing: A critical and appraisal. CBS Publ. Distributers, New Delhi, India. pp. 81-118.
Hoseini, H., and M. Nasiri Mahalati. 2006. Effects of pretreatment on germination of seeds of lentil genotypes. (In Persian). J. Agric. Res. 4(1): 35-47
International Seed Testing Association (ISTA). 1979. International Seed Testing Association. The germination test. Seed. Sci. Technol. 4: 23-28
International Seed Testing Association (ISTA). 1985. International Seed Testing Association. International Rules، for Seed Testing، Seed Sci. Technol.13: 299-513
International Seed Testing Association (ISTA) 2008. Hand book for Seedling evaluation (3rd. ed). Inter Seed Testing Asoc (ISTA), Zurich, Switzerland
International Seed Testing Association (ISTA). 2010. International Seed Testing Association. Rules Proposals for the International Rules for Seed Testing 2010 Edition OM approved 2009-06-18.doc Approved by OM 2009-06-18 Seed Sci. Technol., 47 pp
Int. Seed Testing Asoc. (ISTA). 2012. International Rules for Seed Testing. Pub. The Int. Seed Testing Asoc. Zurich, Switzerland.
Jahanbakhshi, A., A. Ebdali, M. Sharafizadeh, and B. Habibi Khaneyani. 2012. Osmo-priming effects on germination and yield of the vetch under exhaustion. (In Persian) J. Plant Physiol., Islamic Azad Univ. Ahvaz Agron. 4(16):19-32
Khajeh-Hosseini, M., A.A. Powell, and I.J. Bingham. 2003. The interaction between salinity stress and seed vigour during germination of soybean seeds. Seed. Sci. Technol. 31: 715-725.
Khazaee, H., A. Nezami, M. Dashti, and H. Mehrabadi. 2010. Triticale seed priming effect on seed germination characteristics in terms of salinity. (In Persian). J. Modern Agric. 4(4): 303-318
Khoshkharam, M., M. Shahrajabeyan, A. Soleimani, and G. Fathi. 2010. Burnout effect on seed germination and seedling growth components 2 safflower varieties. (In Persian). Fifth Natl. Conf. New Ideas. Agric. Islamic Azad Univ. 1-3
Machado Neto, N.B., C.C. Custodio, and M. Takaki. 2001. Evaluation of naturally and artificially aged seeds of Phaseolus vulgaris L., Seed. Sci. Technol. 29: 137-149.
Maguire, J. D. 1962. Speed of germination in selection and evaluation for seedling vigor. Crop Sci. 2: 176-177.
Martínez, F., A. Pacheco, C. Aguilar, G. Pardo, and E. Ortiz. 2014. Effects of magnetic field irradiation on broccoli seed with accelerated aging. Acta. Agrophys., 2014, 21(1), 63-73
Matthews, S., and A.A. Powell. 1987. Controlled deterioration test.p 49-56. In Handbook of vigor test methods. F. Fiala (ed), ISTA, Zurich, 2nd edition.
McDonald, M.B. 2001. Seed determination: physiology, repair and assessment. Seed Sci. Technol. 27: 177-237.
Mohajeri, F. Ramroudi,M. Taghvaei, M., and Galavi, M. 2015a. Critical concentration of calcium chloride and potassium chloride to pretreatment seed. The Third National Con Iran Environ. Agric. Res. (In Persian).
Mohajeri, F. Taghvaei, M. Ramroudi, M., and Galavi, M. 2015b. The Evaluation of the Responses of Pinto Bean (Phaseolus vulgaris L.) Seedling Emergence and Growth to Different Seed Priming. J. Agric. Sci. 7(7): 235-242
Mohammadi, H., A. Soltani, S. Sadeghpoor, A. Zeinali, and J. Najafi. 2006. The impact on growth and chlorophyll fluorescence in soybean seed deterioration, (In Persian). J. Agric. Sci. Nat. Res., 5: 125-128
Musa, A.M., J. Johansen, J. Kumar, and D. Harris. 1999. Response of chickpea to seed priming in the high Barind Tract of Bangladesh. Int. Chickpea Pigeon pea. Newsl. 6:20-22.
Penalosa. A., and M. Eira. 1993. Hydration dehydration treatment on tomato seeds (Lycopersicon esculentum Mill). Seed Sci. Technol. 21:309-316.
Powell, A.A. 1998. Seed improvement by selection and invigoration. Sci. Agric. Piracicaba. 55: 126-133.
Sadeghi, M., M. Esfahani, and A. Jolodar. 2007. Exhaustion effects on seed germination characteristics of rapeseed, (In Persian). Natl. Conf. Soil, Water, Plants. Agric. Mechanization. Islamic Azad Univ. Dezful. 15-18
Soltani, A., B. Kamkar, S. Ghaleshi, and F. Ghaderi. 2009. Deterioration effect on seed germination of wheat in response to environmental stresses. (In Persian). J. Crop. Prod. 2(2): 43-58
Zomrodi, M., M. Khosravi, M. Khajeh-Hoseini, M. Gheshm, and S. Anvarkhah. 2012. Effect of Priming and Aging on seed germination and vigor artificial seed (Lycopersicum esculentum L.) Tomatoes. (In Persian). J. Appl. Res. Ecophysiol. Plants. 1(1): 97-114.