Effects of Eucalyptus camaldulesis aquatic leaf extract on Sorghum halapense seed germination, antioxidants enzyme and α-amylase enzyme activities

Document Type : Original Article

Authors

1 Associate Professor/ Department of Weed Science, Shoushtar branch, Islamic Azad University, Shoushtar, Iran.

2 Post-Graduated/ Department of Weed Science, Shoushtar branch, Islamic Azad University, Shoushtar, Iran.

Abstract

In order to evaluate the allelopatic potential of Eucalyptus camaldulesis aquatic extract on antioxidant enzyme activities, cell membrane damage and α-amylase enzyme activity of S. halapense, thisexperiments was conducted in Islamic Azad University, Shoushtar branch at 2012. The experiment was laid out according to a Completely Randomized Design with five replications and six treatments were various concentration of E. camaldulesis aquatic extract (0, 10, 20, 30, 40, 50 and 60%). The results indicated E. camaldulesis aquatic extract application exhibited gradual rise inhibitory effect on seed germination, seedling fresh weight, antioxidants enzymes activities, and α-amylase enzyme activity. Contrary to the previous traits, elevated malondialdehyde concentration and seedling fatty acid in S. halapense seedlings were detected. The minimum α-amylase enzyme activity (3.1 and 3.5  nmol prot -1 min-2 ), seedling fresh weight (0.1 and 0.8 mg) and seed germination (54% and 42 %) showed in 40% and 50% Eucalyptus camaldulesis aquatic extract.  The highest fatty acid (23.6% and 23.1%) was noted at 40% and 50% Eucalyptus camaldulesis aquatic extract. In conclusion, E. camaldulesis aquatic extract decreased seedling growth, α-amylase enzyme activity and cell membrane stability of S. halapense seedling. 

Keywords

References

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