The effect of nano-particle silicon dioxide (SiO2) on improving soybean seed germination under Accelerated aging conditions

Document Type : Original Article

Authors

1 Master student/ Department of Seed and Technology/ Faculty of Agriculture, Shahed University, Tehran, Iran.

2 Assistant Professor/ Agricultural College and Medicinal Plant Research Center, Shahed University, Tehran, Iran.

Abstract

The experiment was conducted to examine the effects of nano-particle silicon dioxide to improve germination of deteriorated soybean seeds (Williams). The experiment was done as factorial in a completely randomized design with three replications in 2015 in Seed Technology Laboratory in the University of Shahed. The first factor involves different levels of accelerated aging including control and accelerated aging for 24 and 48 hours at 41 ° C in high humidity conditions and different concentrations of nano-silica as the second factor including zero (control), 40 and 60 ppm, respectively. The traits such as germination percentage, germination rate, the average duration of germination, the number of normal seedlings, seedling fresh weight, root to shoot ratio, relative water content of root, seedling length and chlorophyll content were measured. The results showed that the effect of nano-silica pre-treated soybean seed germination was significant on all traits. But the interaction of nano-silica priming and the deteriorated seeds of soybean was significant only in some germination characteristics. Pre-treatment of 60 ppm nano-particle silica increased 20% of deteriorated seeds germination rate and reduced the average time of germination. Pre-treatment of 40 ppm increased about 7-10% of the number of normal seedlings, and also treatment of silica nanoparticles improved seed germination with compensate amount of chlorophyll in deteriorated seeds.

Keywords


Ajouri, A., A. Haben, and M. Becker. 2004. Seed priming enhances germination and seedling growth of barley under conditions of P and Zn deficiency. Plant Nutr. Soil Sci.
Arnon, A. N. 1967. Method of extraction of chlorophyll in the plants. Agron. J. 23:112-121.
Bars, S. M. A., N. Ahmad, M. Khan, M. M., N., and M. A. Cheema. 2003. Assessment of cottonseed deterioration during accelerated ageing. Seed Sci. Technol. 3:531-540.
Bedi, S. R. Kaur, J.S. Sital, and J. Kaur. 2006. Artificial ageing of Brassica seeds of different maturity levels. Seed Sci. Technol. 34 (2): 287 – 296.
Dell’ Aquila, A., and M. Di Turi. 1996. The germination response to heat and salt stress in evaluating vigor loss in aged wheat seeds. Seed Sci. Technol. 24:309-319.
Ellis, R. H. and E. H. Roberts. 1981. The quantification of ageing and survival in orthodox seeds. SeedSci. Technol.9: 377-409.
Goel A, and I. Sheoran. 2003. Lipid peroxidation and peroxide-scavenging enzymes in cotton seeds under natural ageing. Bio. Plant.
Guan, Y. J., J. Hu., X. J. Wang., and C. X. Shao. 2009. Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. Zhejiang Univ-Sci. 10: 427-433.
Haghighi, M., Z. Afifipour., and M. Mozafarian. 2012. The effect of N-Si on tomato seedgermination under salinity levels. J. Biodi and Envi Sci. 6 (16), 87–90.
Haghighi, M., and M. Pessarakli. 2013. Influence of silicon and nano-silicon on salinity tolerance of cherrytomatoes (Solanum lycopersicum L.) at early growth stage. Scientia Horticul. 161: 111–117.
Harrington, J. F. (1973). Biochemical basis of seed longevity. Seed Sci. Technol. 1:453-461.
Holaday, A.S., S.W. Ritchie., and H.T. Neguyen. 1992. Effect of water deficit on gas exchange parameters and ribulose 15-bisphosphate carboxylase activation in wheat. Environ. Exp. Bot. 32:403-409.
Khajeh-Hosseini, M., A. A. Powell, And I. J. Bingham. 2003. The interaction between salinity stress and seed vigor during germination of soyabean seeds. Seed Sci. Technol. 31: 715- 725.
Krishnan, P., S.Nagarajan, M. Dadlani, and A. V. Moharir. 2003. Characterization of wheat (Triticum aestivum) and soybean (Glycine max) seeds under accelerated ageing Conditions by proton nuclear magnetic spectroscopy. Seed Sci. Technol. 31:541- 550.
Krishnan, P., S. Nagarajan, M. Dadlani, and A.V. Moharir. 2003. Characterization of wheat (triticum aestivum) and soybean (Glycine max) seeds under accelerated ageing Conditions by proton nuclear magnetic spectroscopy. Seed Sci. Technol. 31:541- 550.
Lee, C. W., S. Mahendra, K. Zodrow, D. Li, Y. Tsai, J. Braam and P. J. J. Alvarez. 2010. Developmental phytotoxicity of metal oxide nanoparticles to Arabodopsis thaliana. Environ. Toxicol. Chem. 29: 669-675.
Liopa-Tsakalidi, A., G. Kaspiris, G.Salahas, and P. Barouchas. 2012. Effect of salicylic acid (SA) and gibberellic acid (GA1) pre-soaking on seed germination of Stevia (Stevia rebaudiana) under salt stress. J. Med. Plants Res, 6: 416-423.
Mansour M. M. F. 1994. Changes in growth osmotic potential and cell permeability of wheat cultivars under salt stress. Biol. Plantarum, 36(3): 429-434.
Marshal, A. H., and D. N. Lewis. 2004. Influence of seed storage conditions on seedling emergence, seedling growth and dry matter production of temperate forage grasses. Seed Sci. Technol. 27:177- 237.
McDonald, M. B. 1999. Seed deterioration; physiology, repair and assessment. Seed Sci. Technol. 27: 177-180.
Mohammadi, H. 2013. The role of priming on seed reserve utilization and germination of barley (Hordeum vulgare L.) seeds under drought stress. Int. J. Agron. Plant Prod. 4 (10): 2543-2547.
Mukhopadhyay, M., M.Choudhuri, K. Sen, and B. Ghosh. 1983. Changes in polyamines and related enzymes with loss of viability in rice seeds. Phytochem. 22: 1547-1551.
Murthy, U.M.N., P.D. Kumar., and W.Q. Sun. 2003. Mechanisims of seed aging under different storable conditions for vigina vadiata (L.) wilczek: lipid peroxidation, sugar hydrolysis, Maillavd rections and their relationship to state transition. J. Exp. Bot. 54.384:1057-1067.
Nellist, M. E. and M. Hughes. 1973. Physical and biological processes in the drying of seed. Seed Sci. Technol.1:613-643.
Noor, E., F. M. Azhar., and A. L. Khan. 2001. Differences in responses of gossypium hirsutum L. varieties to NaCl salinity at seedling stage. International J. Agric. Biologic. 3(4): 345-347.
Omidi H., A., A. Sorushzadeh., and F. Ghezeli. 2005. Evaluation of priming pretreatments on germination rapeseed. Agric. Sci. Technol. 19(2): 1-10. (In Persian, with English Abstract).
Pagter, M., C. Bragato., M. Malagoli, and H. Brix. 2009. Osmotic and ionic effects of NaCl and Na2SO4 salinity on Phragmites australis. Aquat. Bot. 90: 43-51.
Pourkhaloee, A., M. Haghighi., M.J. Saharkhiz., H. Jouzi., and M.M. Doroodmand. 2011. Investigation on the effects of carbon nanotubes (CNTs) on seed germinationand seedling growth of salvia (Salvia microsiphon), pepper (Capsicum annum) and tall fescue (Festuca arundinacea). J. Seed Technol. 33 (2), 155–160.
Salvucci, M.E., and S.J. Crafts-Brandner. 2004. Inhibition of photosynthesis by heat stress: the activation state of Rubisco as a limiting factor in photosynthesis. Phys. Plant. 120:179-186.
Shi, Y., W. Yichao., J. F. Timothy., and G. Haijun. 2013. Silicon decreases chloride transport in rice (Oryza sativa L.) in saline conditions. J. Plant Phys. 170: 847- 853.
Susana, p., and B. Alberto. 1990. Effect of natural and accelerated aging on the hydro peroxide metabolism of soybean embryonic axes. Plant Sci. 68 (1990) 27-32.
Zhu, Z, G. Wei., J. Li., Q. Qian., and J. Yu. 2004. Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt-stressed cucumber (Cucumis sativus L.). Plant Sci. 167: 527-533.