Effect of different seed deterioration treatments and germination under different temperatures on the activity of antioxidant enzymes and lipid peroxidation in Pumpkin (Cucurbita pepo L.) seedlings

Document Type : Original Article

Authors

1 Former M.Sc. student of Seed Science and Technology/Dept. of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh ardabilli, Ardabil, Iran.

2 Prof./ Dept. of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabilli, Ardabil, Iran.

Abstract

 In order to investigate the effects of seed deterioration on the activity of antioxidant enzymes and lipid peroxidation in pumpkin seedlings a factorial experiment conducted based on completely randomized design with three replications. Treatments were three deterioration levels (control, 5 and 10 days deterioration at 45◦C and 95-100% relative humidity) and three germination temperatures (15, 25 and 35°C). Results showed that activity of superoxide dismutase and catalase were the highest at 5 days aging and 25°C, and activity of peroxidase was the highest at non-aging and 25°C. Also, the highest amount of Malondialdehyde (7.1 mmol gr-1FW) observed in the seeds with 10 days deterioration and 15°C. The highest respiration index (0.018 mg) achieved in 5 days deterioration and 35°C and the lowest value (0.009 mg) was related to 10 days deterioration and 35°C. It seems that reduction in seed vigor under deterioration and high temperatures causes to oxidative stress and lipid peroxidation and leads to increase in the activity of antioxidant enzymes in order to face the related damages. Considering results obtained from germination and antioxidant enzymes activity the best temperature for germination of aged seeds of pumpkin was 15◦C.

Keywords

References

Abi, H. 1984. Catalase in vitro. Method of Enzymology, 105:121-126.
Aruyi, H., M. Azizi Arani, and A. Emami. 2007. Effect of planting date on the grain yield and oil content in medicinal pumpkin. Final research report. No. 2. Department of Horticulture. Ferdowsi University of Mashhad.
Asada, K. 1994. Production and action of active oxygen species in photosynthetic tissues. In: Foyer CH and Mullineaux PM, eds. Causes of photooxidative stress and amelioration of defense systems in plants. London: CRC Press. 77-104.
Basra, S.M.A., N. Ahmad, M.M. Khan, N. Iqbal, and M.A. Cheema. 2003. Assessment of cotton seed deterioration during accelerated ageing. Seed Sci. Technol. 31: 531-540.
Bhattachrjee, S. 2005. Reactive oxygen species and oxidative burst: roles in stress, senescence and signal transduction in plant. Curr. Sci. 89: 1113-1121.
Bowler, C., M. Van Montagu, and D. Inzé. 1992. Superoxide dismutases and stress tolerance. Ann. Rev. Plant Physiol. Plant Mol. Biol. 43: 83-116.
Cakmak, T., O. Atici, G. Agar, and S. Sunar. 2010. Natural aging- related biochemical changes in alfalfa seeds stored for 42 years. Int. Res. J. Plant Sci. 1(1): 1-6.
Copeland, L.O., and M.B. McDonald. 1985. Seed vigor and vigor tests. In principles of Seed Science and Technology. Second Edition, McMilan Publishing Company, New York. U.S.A. pp. 121-144.
De Figueiredo, E., M.C. Albuquerque, and N.M. De Carvalho. 2003. Effect of the type of environmental stress on the emergence of sunflower (Helianthus annus L.), soybean (Glycine max L.) and maize (Zea mays L.) seeds with different levels of vigor. Seed Sci. Technol. 31:465-479.
Desikan, R., S.A.H. Mackerness, J.T. Hancock, and S.J. Neill. 2001. Regulation of the Arabidopsis transcriptome by oxidative stress. Plant Physiol. 127: 159-172.
Dornbos, D.L., Mullen, R. E., Shibles, R.M. 1989. Drought stress effects during seed filling on soybean seed germination and vigour. Crop Sci. 29: 476-480.
Ellis, R.H., and E.H. Roberts. 1980. Towards a rational basis for testing seed quality. In: P.D. Hebblethwaite (ed.) Seed Production. Bufferworths, London. pp. 605-635.
Ghasemi Golezani, K., V. Salehian, F. Rahimzadeh Khoie, and M. Moghaddam. 1996. Effect of seed vigor on seedling emergence and grain yield of wheat. J. Agric. Sci. Nat. Res. 3: 48-54. (In Persian, with English Abstract).
Ghassemi-Golezani, K., S. Khomari, B. Dalili, A. Hosseinzade-Mahootchy, and A. Chadordooz-Jeddi. 2010. Effects of seed aging on field performance of winter oilseed rape. J. Food Agric. Environ. Sci. 8: 175-178.
Giannopolitis, C.N., and S.K. Ries. 1977. Superoxide dismutase. I. Occurrence in higher plants. J. Plant Physiol. 59: 309-314.
Hasan, M. A., J.U. Ahmed, M.M. Hossein, and M.A. Ullah. 2004. Germination characters and seed reserve mobilization during germination of different wheat genotypes under variable temperature regimes. J. Nat. Sci. Found. Sri Lanka. 32:97-107.
International Seed Testing Association. International rules for seed testing (supplement). Seed Sci. Technol. 27: 1-333.
Jordan, G.L., and M.R. Haferkamp. 1989. Temperature responses and calculated heat units for germination of several range grasses and shrubs. J. Range Manage. 42:41-45.
Kaewnaree, P., S. Vichitphan, P. Klanrit, B. Siri, and K. Vichitphan. 2011. Effect of accelerated aging process on seed quality and biochemical changes in sweet pepper (Capsicum annuum L.). Seed Biotechnol. 10(2): 175-182.
Kato, M., and S. Shimizu. 1987. Chlorophyll metabolism in higher plants. VII. Chlorophyll degradation in senescing tobacco leaves: phenolic dependent peroxidative degradation. Can. J. Bot. 65: 729–735.
Kusvuran, S., S. Ellialtioglu, and Z. Polat. 2013. Antioxidative enzyme activity,lipid peroxidation, and proline accumulation in the callus tissues of salt and drought tolerant and sensitive pumpkin genotypes under chilling stress. Hortic. Environ. Biotechnol. 54(4): 319-325.
Magbanua, Z.V., C.M.D. Moraes, T.D. Brooks, W.P. Williams, and D.S. Luthe. 2007. Is catalase activity one of the factors associated with maize resistance to Aspergillus flavus. Mol. Plant Microbe Interact. 20(6): 697–706.
Marshal, A.H., and D.N. Lewis. 2004. Influence of seed storage conditions on seedling emergence, seedling growth and dry matter production of temperate forage grasses. Seed Sci. Technol. 9:493-501.
McCue, P., and K. Shetty. 2002. A biochemical analysis of Mungbean (Vigna radiata) response to microbial polysaccharides and potential phenolic-enhancing effects for nutraceutical applications. Food Biotechnol. 16:57–79.
McDonald, M.B. 1999. Seed deterioration: Physiology, repair and assessment. Seed Sci. Technol. 27: 177-237.
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7: 405-410.
Mittler, R., S. Vanderauwera, M. Gollery, and F. Van Breusegem. 2004. Reactive oxygen gene network of plants. Trends Plant Sci. 9(10): 490-498.
Murthy, U.M.N., Y. Liang, P.P. Kumar, and W.Q. Sun. 2002. Non-enzymatic protein modification by the Maillard reaction reduces the activities of scavenging enzymes in Vigna radiate. Physiol. Planta. 115: 213–220.
Omid Beygi, R. 1995. Principles of production and processing of medicinal plants. Fekr Ruz Publications. P: 283. (In Persian).
Perry, D. A. 1980. The concept of seed vigour and its relevance to seed production techniques. In: P. D. Hebblethwaite (ed.). Seed production. Butterworths, London, pp. 585-591.
Quiroga, M., C. Guerrero, M.A. Botella, A. Barcelo, I. Amaya, M.I. Medina, F.J. Alonso, S.M. De Forchetti, H. Tigier, and V. Valpuesta. 2000. A tomato peroxidase involved in the synthesis of lignin and suberin. Plant Physiol. 122: 1119-1127.
Ram, C., and L.E. Weisher. 1988. Effect of artificial aging on physiological biochemical parameters of seed quality in wheat. Seed Sci. Technol. 16:579-587.
Sairam, R.K., K.V. Rao, and G.C. Srivastava. 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Sci. 163:1037-1046.
Sevengor, S., F. Yasar, S. Kusvuran, and S. Ellialaltioglu. 2011. The effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidative enzymes of pumpkin seedling. Afr. J. Agric. Res. 6(21): 4920-4924.
Singh Gill, S., and N. Tuteja. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants: A Review. Plant Physiol. Biochem. 48: 909-930.
Soltani, E., B. Kamkar, S. Galeshi, and F. Akram Ghaderi. 2008. The effect of seed deterioration on seed reserves depletion and heterotrophic seedling growth of wheat. J. Agric. Sci. Nat. Resour. 15(1): 13-17. (In Persian, with English Abstract).
Sunkar, R. 2010. Plant stress tolerance (Methods and Protocols). Department of Biochemistry and Molecular Biology. 401p.

Files

Share

How to cite

Statistics