Effect of Salicylic Acid and Drought Stress on Germination and Activity of Antioxidant Enzymes of Barely

Document Type : Original Article

Author

Assistant Prof./ Seed and Plant Certification and Registration research Institute (SPCRI), Karaj, Alborz, Iran.

Abstract

In order to study effectpretreatment salicylic acid under drought stress on germination characteristics this research was conducted in May 1393. Barley seed varieties Zahak after soaking in solution [zero (water) 0.7 1.2 and 2.7 mM ) of salicylic acid (SA) for 24 hours under conditions of drought stress on the germination dishes. Containing 10 ml of a solution of polyethylene glycol (6000) with potentials [zero (control), -0.5 MPa, 1 MPa and 1.5 MPa] moved, and related traits were determined germination. The results showed that the maximum effect of salicylic acid on germination time, reduce stress levels were low. The highest germination percentage (93 %) and the effective yield of seed soaking with the minimum concentration of salicylic acid 0. 7 mM ) and low levels of stress were gained. Vigor and Seedling vigor index at high concentrations of salicylic acid and high levels of stress decreased. In this study, drought stress reduced the germination traits and salicylic acid reduces the adverse effect of drought stress on germination was related traits.

Keywords


Amor, N.B., K.B. Hammed, A. Debez, C. Grignon, and, C.  Abdely. 2005. Physiological and antioxidant responses of the perennial halophyte Crithmummmaritimum to salinity. Plant Sci. 168,889-899.
Ashraf, M. and M. R. Foolad. 2005.Pre–sowing seed treatment – A shotgun approach to Improve germination, growth and crop yield under saline andnon – saline conditions. Adv. Agron. 88: 223-265
Bradford, M. 1976. A rapid and sensitive method for the quantitation of protein utilizing the principle of protein- dye binding.Annu. Rev. Biochem. 72: 248 - 254.
Cakmad, I., and W. Horst. 1991. Effect of aluminum on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tip of soybean (Glycine max). Plant Physiol.83: 463- 468.
Cut, J.R., and D.F. Kelssig. 1992. Salicylic acid in plants: A changing perspective. Pharmacy Technol. 16: 25-34.
Ellis, R.H., and E.H. Roberts. 1981.The quantification of ageing and survival in orthodox seeds. Seed Sci. Technol. 9: 377-409.
Gallardo, K., J. Claudette, SPC. Groot, M. Puype, J. Vandekerckhove, and D. Job .2001.Proteomic analysis of Arabidopsis seed germination and priming. Plant Physiol. 126: 835-848
Deef, H.E. 2007. Influence of salicylic Acid on stress tolerance during seed germination of Triticumaestivum and Hordeumvulgare. Adv. Biol. Res.1: Pp : 40-48.
Hunter, E. A., C. A. Glasbey, and R. E. L. Naylor. 1984. The analysis of data from germination tests. J. Agric. Sci.102:207-213.
Maguire, J.D. 1962. The seed of germination – aid in selection and evaluation for seedling emergence and vigor. Crop Sci. 2: 176-177.
McDonald, M.B., and L.O. Copeland,2000.Seed production, principles and practices. Chapman and Hall, U.S.A. pages, 385
Scott, S.J., R.A. Jones, and W.A. Williams. 1984. Review of data analysis methods for seed germination. Crop Sci. 24: 1192-1199.
Senaratna, T, D. Touchell, E. Bunn and K. Dixon. 2000. Acetylsalicylic acid (Aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plant. Plant Growth Regul. 30, 157-161.