Study on the effect of priming on the activity of antioxidant enzymes in hybrid maize single cross 704 under drought stress

Document Type : Original Article

Authors

1 Former M.Sc. student of Seed Science and Technology/ Agronomy and Plant Breedign Dept., Univ. of Mohaghegh Ardabilli, Ardabil, Iran.

2 Associate Prof./ Dept. of Agronomy, Faculty of Agricultural Sciences & Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.

Abstract

In this research effect of priming types was investigated on the activity of antioxidant enzymes including Catalase (CAT), Ascorbate peroxidase (APX), Superoxide dismutase (SOD), Malone dialdehyde (MDA) and Gluthation reductase (Gred) in hybrid maize single cross 704 under drought stress. Treatments were drought at 4 levels (0, -3, -6 and -9 bar) and priming (Hormone, hydro, osmo, ascorbic and control). Germination percentage, seedling vigor and the activity of antioxidant enzymes were measured. Results showed that interaction of drought and priming was significant an all traits except of germination percentage. The highest seedling vigor (15.996) obtained from control condition. The highest CAT activity (35 μmol min-1 mg-1) observed in ascorbic acid priming at -9 bar drought stress. Also, the highest APX activity was related to hormone priming and -9 bar. While the lowest activity observed in control drought and osmo-priming. The highest amount of MDA achieved in -9 bar and control priming. The highest SOD activity observed in -9 bar. The highest Gred activity obtained by ascorbic acid priming at -9 bar.

Keywords


Abi, H. 1984. Catalase in vitro. Method of Enzymology, 105:121-126.
Abdul-baki, A.A., and J.D. Anderson. 1973. Vigor determination in soybean seed by multiplication. Crop Sci. 3: 630-633.
Akram Ghaderi, F., B. Kamkar, and A. Soltani. 2008. Seed Science and Technology. Publications University of Mashhad. (In Persian).
Ananeiva, D.H., C. Rusterucci, B.F. Holt, R.A. Dietrich, J.E. Parker, and J.L. Dangl. 2002. Runaway cell death, but not basal disease resistance, in Isd1 is SA- and NIM1/NPR1-dependent. Plant J. 29: 381–391.
Anonymous. 2010. International rules for seed testing. International seed testing association (ISTA).
Ariano, S., D. Bartolomeo, X. Cristos, and M. Andras. 2005. Antioxidant defenses in Olive trees during drought stress: changes in activity of some antioxidant enzymes. Functional Plant Biol. 32: 45-53.
Ashraf, M, and M.R. Foolad. 2005. Pre-sowing seed treatment a shotgun approach to improve germination growth and crop yield under saline and none-saline conditions. Adv. Agron. 88: 223-271. (In Persian, with English Abstract).
Ataie Sheikh, A. 2004. Evaluation of drought stress on some physiological characteristics and activity of antioxidant enzymes  in different varieties of pea. M.Sc. Thesis. Islamic Azad university. Karaj branch.
Bailly, C. 2004. Active oxygen species and antioxidants in seed biology. Seed Sci. Res.14:93-107.
Basra, S.M.A., I.A. Pannu, and I. Afzal. 2003. Evaluation of seedling vigour of hydro and matriprime wheat ( Triticum aestivum L.) seeds. Int. J. Agric. Biol. 5:121- 125.
Bohnert, H.J, and R.G. Jensen. 1996. Strategies for engineering water stress tolerance in plants.Trends in Biotechnol. 14: 89-97.
Bowler, C.,  M. Van Montagu, and D.  Inzé. 1992. Superoxide dismutases and stress tolerance. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43: 83-116.
Burguieres, E., P. McCu, Y. Kwon, and K. Shetty. 2007. Effect of vitamin C and folic acid on seed vigour response and phenolic-linked antioxidation activity. Biores. Technol. 98: 1393-1404.
Chiu, K.Y.,  C.L., Chen, and J.M.  Sung. 2002. Effect of priming temperature on storability of primed sh-2 sweet corn seed. Crop Sci. 42.1996-2003.
Cruzde Carvalho, M.H. 2008. Drought stress and reactive oxygen species. Plant Signal. Behav. 3: 156-165.
Dadnia, M.R. 2005. The effect of water shortage on physiological and agronomical characteristics of Sunflower varieties. PhD Thesis. Islamic Azad university. Ahvaz branch.
De Figueiredo, E., M.C., Albuquerque, and N.M. de Carvalho. 2003. Effect of the type of environmental stress on the emergence of sunflower (Helianthus annus L.), soybean (Glycine max L.) and maize (Zea mays L.) seeds with different levels of vigor. Seed Sci. Technol. 31:465-479.
Demir Kaya, M., O. Gamze, M. Atak, Y. Çikili. and  O. Kolsarici. 2006. Seed treatment to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Eur. J. Agron. 24: 291-295.
Dolatabadian, A., S.A.M., ModarresSanavy, and M. Sharifi. 2009. Effect of Water Deficit Stress and Foliar Application of Ascorbic acid on Antioxidants Enzymes Activity and Some Biochemical's Changes in Leaves of Grain Corn (Zea maize L.). 22(3): 407-422[M1] .
Esterbauer, H., and D. Grill. 1978. Seasonal variation of glutathione and glutathione reductase in needles of Piceaabies. Plant Physiol. 61:119–121.
Ghasemi-Golezani, K., A.A., Aliloo, M. Valizadeh, and M. oghaddam. 2008. Effects of Hydro and Osmo-Priming on Seed Germination and Field Emergence of Lentil (Lens culinarisMedik.). Notulae Botanicae Horti. Agrobot. 36(1): 29-33.
Ghorbani Ghojdi, H, and A.R. Ladan-Moghaddam. 2005. Introduction to oxidative stresses and plant strains. Davavin Tehran. 128 p.
Giannopolitis, C.N, and S.K. Ries. 1977. Superoxide dismutase. I. Occurrence in higher plants. J. Plant Physiol. 59: 309-314.
Habibi, D., S. Orojnia, D. Taleghani, A.R. Pazoki, and M. Davodifard. 2012. Assessment of changes in antioxidant enzymes and the performance of different genotypes of sugar beet under drought stress. J Agron. Plant Breed. 8(4): 63- 82.
Hadad, R, and M. Salek Jalali. 2009. Protein changes and antioxidant enzymes activity on barley inbred lines under water shortage. Plant Prod. Technol. 9(2): 1- 10.
International Seed Testing Association. 2008. International rules for seed testing (supplement). Seed Sci. Technol. 27: 1-333.
Ito, H., H. Nobutusgu, and A. Ohbayashi. 1991. Purification and characterization of rice peroxidase. Agric. Biol. Chem. 55(10): 2445-2454.
Jabari, F., A. Ahmadi, K. Poustini, and H. Alizadeh. 2006. Evaluation of some antioxidant enzymeeffects on chlorophyll and cell membrane in drought susceptible and tolerant wheat varieties. Iranian J. Agric. Sci. 37: 307-316.
Jiang, Y., and B.  Huang. 2001. Drought and heat stress injury to two cool-season turfgrasses in relation to antioxidant metabolism and lipid peroxidation. Crop Sci. 41: 436–442.
Jose, M, and G.  Mates eristinaperez. 1999. Antioxidant Enzymes and Human Disease. Chem. Biochem. 32(8): 595 – 603.
Jung, S. 2004. Variation in antioxidant metabolism of young and mature leaves of Arabidopsis thaliana subjected to drought. Crop Sci. 166: 459-466.
Kafi, M., A. Zand, B. Kamkar, H.R. SHarifi, and M. GHoldani. 2005. Plant Physiology. Mashhad University Jehad.
Kazemi Arbat H. 2007. Private agronomy. Center Publication University. 315 p. (In Persian).
Kidd, F, and C. West. 1918. Physiological predetermination: The influence of the physiological condition of the seed upon the course of subsequent growth and upon the yield. I. The effect of the soaking seeds in water. Ann. Appl. Biol. 5: 1-10.
Kusvuran, S., S. Ellialtioglu, and Z. Polat. 2013. Antioxidative Enzyme Activity,Lipid Peroxidation, and Proline Accumulation in the Callus Tissues of Salt and Drought Tolerant and Sensitive Pumpkin Genotypes under Chilling Stress. Hortic. Environ. Biotechnol. 54(4): 319-325.
Liang, Y.C. 1999. Effects of silicon on enzyme activity and sodium, potassium and calcium concentration in barley under salt stress. Plant Soil. 209: 217-224.
Manochehrifar, P., H.  Lari Yazdi, and B. Zagi. 2013. Effect of drought stress and salicylic acid on some indicators of germination in two varieties of corn. J. Plant Ecol. 9(1-34): 3- 20.
Maria, C., R. Esther, M. González, F.R. Minchin, K. Judith, W.C. Arrese-Igor, J. Ramos, and M. Becana. 2002. Effects of water stress on antioxidant enzymes of leaves and nodules of transgenic alfalfa overexpressing superoxide dismutases. Physiologia Plantarum. 115: 531–540.
McCue, P, and K. Shetty. 2002. A biochemical analysis of Mungbean (Vignaradiata) response tomicrobial polysaccharides and potential phenolic-enhancing effects for nutraceutical applications. Food Biotechnol.16:57–79.
McDonald, M.B. 1999. Seed deterioration: Physiology, repair and assessment. Seed Sci. Technol. 27: 177-237.
McDonald, M.B. 2000. Seed priming. (eds. M. Black and J. D. Bewley). Sheffield Academic press.
Michel, B.E, and  M.R. Kaufmann. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiol. 51: 914-916.
Mittler, R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends in Plant Sci. 7: 405-410.
Moaveni, P., and M. CHangizi. 2007. Principles of crop physiology in dry and saline conditions. Scientific Publications Islamic Azad University Arak.
Moosavi, A., R. Tavakkol-Afshari, F. Sharif-Zadeh, and A. Aynehband. 2009. Effect of seed priming on germination characteristics, polyphenol oxidase, and peroxidase activities of four amaranth cultivars. J. Food Agric. Environ.7: 353-358.
Movahhedi Dehnavi, M., S.A. Modarres Sanavi, A. Soroshzade, and M. Jalali. 2003.  Changes in proline, Soluble sugars, SPAD and Chlorophyll fluorescence in   winter Safflower cultivars under drought stress and Spraying zinc and manganese. Biaban J. 9(1): 93- 110.
Murugu, F.S., P. Nyamugafata, C. Chiduza, L.J. Clark, and W.R. Whalley. 2003.Effects of seed priming, aggregate size and matric potential on emergence of cotton (Gossypiumhirsutum L.) and maize (Zea mays L.).Soil Tillage Res.74:161-168.
Nakano, Y, and K. Asada. 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxides in Spanish chloroplasts. Plant Cell Physiol. 22: 867–880.
Noctor, G, and C. Foyer. 1998.Ascorbate and glutathione: keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49: 249–279.
Ohe, M., M. Rapolu, T. Mieda, Y. Miyagawa, Y. Yabuta, K. Yoshimura, and S. Shigeoka. 2005. Decline in leaf photooxiadtive-stress tolerance with age in tobacco. Plant Sci. 168: 1487-1493.
Pérez-Amador, M., M.L. Abler, E.J. de Rocher, D.M. Thompson, A. Van Hoof, N.D. Lebrasseur, A. Lers, and P.J. Green. 2000. Identification of BFN1, a bifunctional nuclease induced during leaf and stem senescence in Arabidopsis. Plant Physiol. 122: 169-179.
Rohi, A., M. Tajbakhsh, E. Bernose, M.R. Saidi, and P. Nikzad. 2010. Investigation of different pretreatments effects on seed germination and seedling traits of various chickpea (Cicer arietinum L.) cultivars. Agron J. (Pajouhesh and Sazandegi). 90: 1-8.
Sairam, R.K., P.S. Desmuk, and D.S., Shukla. 1997. Tolerance of drought and temperature stress inrelation to increase antioxidant enzyme activity in wheat. J. Agron. Crop Sci. [M2] 178:171-178.
Sairam, R.K., K.V. Rao, and G.C. Srivastava. 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Sci. 163:1037-1046.
Salin, M.L. 1991. Chloroplast and mitochondrial mechanism for protection against oxygen toxicity. Free Radical Res. Commun. 12: 851-858.
Sedghi, M., R. Seyedsharifi, A. Pirzad, and B., Amanpour-Balaneji. 2012. Phytohormonal Regulation of Antioxidant systems in Petals of Drought Stressed Pot Marigold (Calendula officinalis L.). J. Agric. Sci. Technol. 14 (4) :869-878.
Sevengor, S., F. Yasar, S. Kusvuran, and S. Ellialaltioglu. 2011. The effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidative enzymes of pumpkin seedling. Afr. J. Agric. Res. 6(21): 4920-4924.
Soltani, A., E. Zeinali, S. galashi, and N. Latifi. 2001. Genetic variation for and interrelationships among seed vigor traits in wheat from the Caspian Sea Coast of Iran. Seed Sci. Technol. 29: 653-662.
Sung, J.M. 1996. Lipid peroxidation and peroxide-scavenging in soybean seeds during aging. Physiologia Plantarum. 97: 85-89.
Vazan, S., Z. Ranji, M. Hoshdar Tehrani, A. GHalavand, and M. Saneie Shariat Panahi. 2002. Drought stress effect on abscisic acid accumulation and stomatal conductivity of sugar beet. Iranian J. Crop Sci. 4(3): 176-182.
Windauer, L., A. Altuna, and R. Benech-Arnold. 2007. Hydrotime analysis of Lesqueralla fendleri seed germination response to priming treatments. Ind. Crop Prod. 25(1): 70-74.
Zhang, J.X, and M.B. Kirkham. 1994. Droughtstress- induced changes in activities of superoxide dismutase, catalase, and peroxidase in wheat species. Plant Cell Physiol. 35: 785–791.