Effect of water and Poly Amines treatment on barley (Hordeum vulgare L.) c.v Usef seedling vigour and chilling tolerance at harvesting in different seed moisture content

Document Type : Original Article

Authors

1 Ph.D., graduated in Seed Science and Technology, University of Mohaghegh Ardabili

2 University of Mohaghegh Ardabili, Faculty of Agriculture and Natural Resources, Department of Agronomy and Plant Breeding

3 Assoc. Prof./ Seed and Plant Certification and Registration Institute (SPCRI), Karaj-IRAN

4 Assoc. Prof./ Dept. of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.

Abstract

This study was conducted to investigate the  role  of water and polyamines (PAs) as priming  agents on  improving seed vigour and chilling tolerance with  different  moisture  content (Mc)(14.0,16.0,18.0%) under chilling conditions in barley (Hordeum vulgare L.) c.v Usef. Experiments were conducted at the seed and Plant certification and Registration Research Institute in 2013 -2014. The seeds were hand harvested at three initial moisture contents including 18, 16, and 14%  wet weight basis .The seed samples then were sealed in polythene bags and stored in conditioned storage and equilibrium for 8 months. After that for seed priming, seeds were soaked in 20mg/L-1 (w/v) aerated solution of spermine (Spm), Puterscine (Put), and Spermidine (Spd) and distilled water (W) for 16h at 20±2C°. After each treatment, Seeds dried to original moisture level in forced air and sealed in polythene bags, stored in a conditioned storage again until one month. Experimental units were arranged as factorial in a completely randomized design with four replications after treat. The results indicated that Mc18% improved the germination percentage (GP) and seedling vigour compared to Mc 16 and 14%. The electrolyte leakage decreased in seeds harvested by Mc 18% when compared with Mc16 and Mc 14.0%. Also seed treatments significantly increased the seed viability. Likewise, in chilling stress condition maximum proline content and areal dry weight were obtained from Put treat, whereas the lowest electrolyte leakage was recorded for Put treat. Meanwhile, interaction between seed moisture content ×PAs treatment in cold condition significantly affected on plumule length and radicle dry weight, statistically maximum plumule length and radicle dry weight were recorded from Put ×18.0% mc. Also seed priming with water had positive effect on seedling growth after chilling stress.  Generally, the effectiveness of PAs on improving chilling tolerance and increasing seedling vigor was more pronounced in Put treatment along with sample with 18.0% mc.

Keywords

References

Alcázar, R., J.L. García-Martínez, J.C. Cuevas, A.F. Tiburico, and T.Altabella. 2005. Overexpression of ADC2in Arabidopsis induces dwarfism and late flowering through GA deficiency. Plant .J. 43:425-436.
Alexieva, V.1994a. Chemical structure-plant growth regulation activity of some naturally occurring and synthetic aliphatic amines.Compt.Rend.Acad.Bulg.Sci. 47:79-82.
Alexieva, V.1994b. Effect of exogenous Putrescine and its synthetic structural analogues on leaf senescence.Compt. Rend.Acad. Bulg. Sci. 47:57-60.
Bailly, C. 2004. Active oxygen species and antioxidants in seed biology. Seed Sci. Res. 14:93-107.
Bailly, C., C. Audigier, F. Ladonne, M.H. Wagner, F. Coste, F. Corbineau, and D. Côme. 2001. Changesin oligosaccharide content and antioxidant enzyme activities in developing bean seeds as related to acquisition of drying tolerance and seed quality. Journal of Experimental Botany. 52: 701-708.
Bates, L. S., R. P. Waldren, and I. D.Teare. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil .39: 205-207
Basra, A.S., B. Singh, and C.P. Malik, 1994. Priming-in duced changes in polyamine levels in relation to vigor of aged onion seeds.Bot. Bull. Academia Sinica. 35:19-23.
Cao, D.D., J. Hu, C.H. Gao, Y.J.Guan, S. Zhang, and J.F. Xiao. 2008. Chilling tolerance of maize (Zea mays L.) can be improved by seed soaking in Putrescine.Seed Sci. Technol. 36:191-197.
Chinnusamy, V., J. Zhu, and J.K. Zhu. 2007. Cold stress regulation of gene expression in plants. Trends Plant Sci. 12:444-451.
Delouche, J.C.1980. Environment effects on seed development and seed quality. Hort Sci. 15:775-780.
Ellis, R.H. and E.H. Roberts.1981. An investigation into the possible effects of ripeness and repeated threshing on barley seed longevity under six different storage environments. Ann. Bot. 48:93-96.
FAO. FAOSTAT. 2013. Food and Agriculture Organization of the United Nations. Available from: http://faostat.fao.org/faostat, 2013.
Farooq, M., M. Shahzad, A.Basra, H. Rehman, and M. Hussain. 2008. Seed Priming with Polyamines Improves the Germination and Early Seedling Growth in Fine Rice. Journal of New Seeds.  9: 145-155
Farooq, M., T. Aziz, and H.U. Rehman. 2011. Evaluation surface drying and re-drying for wheat seed priming with polyamines: effects on emergence,early seedling growth and starch metabolism. Acta Physiol Plant. 33:1707-1713.
Groppa, M.D., and M.P Benavides. 2008. Polyamines and abiotic stress: recent advance. Amino Acids. 34:35-45.
Guye, M.G., L.Vigh, and J.M.Wilson.1986. Polyamines titer in relation to chilling sensitivity in Phaseolus sp.J. Exp. Bot. 37:1036-1043.
Ha, H.C., Sirisoma, N.S. Kuppusamy, P. Zweller, J.L. Woster, P.M. Casero, and R.A.Jr.1998. The natural polyamine spermine functions as a free radical scavenger. Proc. Natl. Acad. Sci. USA, 95:11140-11145.
Harrington, J.F. 1972. Seed storage and longevity. Pp.145-245. In T.T. Kozlowski.(ed). Seed biology.Vol.III. New York, Academic Press.
Hosseini, N.M. 2006. Cereals Production, 1rd Edition. Nakshe meher, Tehran. (In Persian).
ISTA. 2013. ISTA Handbook on Seedling Evaluation, 3rd Ed. Int. Seed Testing Association, Zurich, Switzerland.
Kasinathan, V. and Wingler, A. 2004.Effect of reduced arginine decarboxylase activity on salt tolerance and on polyamine formation during salt stress in Arabidopsis thaliana. Physiol. Plantarum 121:101-107.
Janská, A., P. Maršík, S. Zelenková, and J. Ovesná. 2010. Cold stress and acclimation-what is important for metabolic adjustment. Plant Biol. 12:395-405.
Kim, T.E., S.K. Kim, T.J. Han, J.S. Lee, and S.C. Chang. 2002. ABA and polyamines act independently in primary leaves of cold–stressed tomato (Lycopersicon esculentum L.). Physiologia Plantarum.115:370-376.
Kocsy, G., M. Pal, A. Soltész, G. Szalai, Á. Boldizsár, and V. Kovács. 2011. (a) Low temperature and oxidative stress in cereals. Acta Agronomic Hungarica. 59: 169-189
Kocsy, G., L. SimonSarkadi, Z. Kovács, Á. Boldizsár, C. Sovány, K. Kirch, and aG. Galiba. 2011. (b) Regulation of free amino acid and polyamine levels during cold acclimation in wheat. Acta Biologica Szegediensis. 55:91-93.
Kuzenetsov, V.V., and N.I. Shevyakova. 2007. Polyamines and Stress Tolerance of Plants. Plant Stress. 1:50-71.
Lee, S.H., A.P. Singh, G.C. Chung, Y.S.Kim, and I.B. Kong. 2002. Chilling root temperature causes rapid ultra structural changes in cortical cells of cucumber (Cucumis sativus L.) root tips.J. Exp. Bot. 53:2225-2237.
Lee, T.M., H.S. Lur, and C. Chu. 1997. Role of abscisic acid in chilling tolerance of rice (Oryza sativa L.) seedling.II. Moudulation of free polyamine levels. Plant Sci. 126:1-10.
Leprince, O., F.J.M. Harren, J. Buitink, M. Alberda, and F.A. Hoekstra. 2000. Metabolic dysfunction and unabated respiration precede the loss of membrane integrity during dehydration of germination radicles. Plant Physiol.122:597-608.
Liu, N., Y.B. Gao, C.X. Jia, and A.Z. Ren. 2000. Changes in POD Activity,free proline content and cytomembrane permeability of Loium multiflorum leaves under different levels of osmotic stress. Plant Physiol. Commun. 36:11-14.
Monroy, A.F., A. Dryanova, B. Malette, D.H. Oren, F.M. Ridha, W. Liu, J.Danyluk, L.W. Ubayasena, K. Kane, G.J. Scoles, F. Sarhan, and P.J. Gulick. 2007. Regulatory gene candidates and gene expression analysis of cold acclimation in winter and spring wheat. Plant Mol. Biol. 64:409-423.
Nadeau, P., S. Delaney,and L. Chouinard. 1987. Effects of cold hardening on the regulation of polyamine levels in wheat (Tritcum aestivum L.) and alfalfa (Medicago sativa L.). Plant Physiol. 84:73-77.
Nayyar, H. 2005. Putrescine increases floral retention,pod set and seed yield in cold stressed chickpea. J. Agron. Crop Sci. 191:340-345.
Németh, M., T. Janda, E. Horváth, E. Páldi, and G. Szalai. 2002. Exogenous salicylic acid increases polyamine content but may decrease drought tolerance in maize. Plant Sci. 162:569-574.
Oufir, M., S. Legay, N. Nicot, K.Van Moer, L. Hoffmann, J. Renaut, J.F. Hausman, and D. Evers. 2008. Gene expression in potato during cold exposure: Changes in carbohydrate and polyamine metabolisms. Plant Sci. 175:839-852.
Öztürk, L., and Y. Demir. 2003. Effects of Putrescine and ethephon on some oxidative stress enzyme activities and proline content in salt stressed spinach leaves. Plant Growth Regul. 40:89-95.
Parvanova, D., S. Ivanov, T. Konstantinova, E. Karanov, A. Atanassov, T. Tsvetkov, V. Alexieva, and D. Djilianov. 2004. Transgenic tobacco plants accumulating osmolytes show reduced oxidative damage under freezing stress, Plant Physiol. Biochem. 42:57-63.
Pillai, M.A., and T. Akiyama.2004. Differential expression of an S-adenosyl-L-methionone decarboxylase gene involved in polyamine biosynthesis under low temperature stress in japonica and indica rice genotypes. Mol. Genetics and Genomics. 271:141-149.
Powell, A.A., S. Matthews, and M. De A. Oliveira.1984. Seed quality in grain legumes. Advances in Appl. Biol.10:217-285.
Prasad, T.K., M.D. Anderson, B.A. Martin, and C.R. Stewart. 1994. Evidence for chilling-induced oxidative stress in maize seedling and a regulatory role for hydrogen peroxide. Plant Cell, 6:65-74.
Rai,V.K. 2002. Role of amino acids in plant responses to stresses. Biol. Plant. 45:481-487.
Rácz, I., M. Kovács, D. Lásztity, O.Veisz, G.Szalai, and E. Páldi. 1996. Effect of short-term and long-term low temperature stress on polyamine biosynthesis in wheat genotypes with varying degrees of frost tolerance.J.Plant Physiol. 148:368-373.
Rajjou, L., M. Duval, K. Gallardo, J. Catusse, J. Bally, C. Job, and D. Job. 2012. Seed Germination and Vigor. Annu. Rev. plant Biol. 63:507-33.
Sãulescu,N.N.,andH.J.Braun.2001.Coldtolerance.pp.111_123.In:Reynolds,M.P.,OrtizMonasterio,J.I.,McNab,A(eds.),Application of physiology in wheat Breeding. CIMIT, Mexico D.F., Mexico.
Stefl, M., I. Trcka, and P.Vratny. 1978. Proline biosynthesis in winter plants due to exposure to low temperatures. Biol. Plant. (Prague) 20:119-128.
Szalai, G., T. Janda, T. Bartók, and E. Páldi. 1997. Role of light in changes in free amino acid and polyamine contents at chilling temperature in maize (Zea mays).Physiol. Plant. 101:434-438.
Szalai, G., M. Pap, and T. Janda, 2009. Light-induced frost tolerance differs in winter and spring wheat plants. J. plant Physiol. 166:1826-1831.
Tekrony, D.M., and D.B. Egli 1997. Accumulation of seed vigour during development and maturation. In: Ellis RH, Black M, Murdoch AJ, Hong TD, eds. Basic and applied aspects of seed biology. Dordrecht: Kluwer Academic Publishers.30:369-384.
Todorova, D., I. Sergiev, and V.Alexieva. 2012. Application of natural and synthetic polyamines as growth regulators to improve the freezing tolerance of winter wheat (Triticum aestivum L.) Acta Agronomica Hungarica. 60: 1-10.
Verma, S. and S.N. Mishra. 2005. Putrescine alleviation of growth in salt stressed Brassica juncea by inducing antioxidative defense System. J. Plant Physiol. 162:669-77.
Xiong, L., K.S. Schumaker, and J.K. Zhu. 2002. Cell signaling during cold, drought, and salt stress. Plant Cell. 14: S165-S183.
Xu, S., J.Hu, Y. Li, W. Ma, Y. Zheng, and S. Zhu. 2011. Chilling tolerance in Nicotiana tabacum induced by seed priming with Putrescine.Plant Growth Regul., 63: 279-290.
Walters, C., D. Ballesteros, and V.A.Vertucci. 2010. Structural mechanics of seed deterioration: standing the test of time. Plant Sci.179:565-73.
Zhang, W., B. Jiang, W. Li, H. Song, Y.Yu, and J. Chen. 2009. Polyamines enhance chilling tolerance of cucumber (Cucumis sativus L.) through modulating antioxidative system. Sci. Hortic. 122:200-208.
Iranian Journal of Seed Science and Technology
Volume 6, Issue 2 - Serial Number 12
Autumn and Winter of 2017-18
November 2017
Pages 213-228

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