Effect of bio-priming on germination and growth of Sugar Beet cultivars (Beta vulgaris L.)

Document Type : Original Article

Authors

1 MSc. Of seed science and technology, Shahrekord Univ., Shahrekord, Iran.

2 Faculty member/ Shahrekord University, Shahrekord, Iran.

3 Faculty member/Shahrekord University, Shahrekord, Iran.

4 Faculty member/ College of Agriculture and Natural Resources,Tehran University, Karaj, Iran.

Abstract

Bio-priming and application of bio-fertilizer has special importance in crop production and sustainable soil fertility. Growth and quality improvement with environment-friendly microorganism have been proved in crops. In order to evaluation of bio-priming possibility in seed improvement of sugar beet cultivars, effects of two bacteria species including Pseudomonas fluorescens and Pseudomonas putida on seed germination and growth indices were evaluated. Experiment was directed as factorial in randomized complete block design with four replication, there in experimental factors were bacteria species including Pseudomonas fluorescens, Pseudomonas putida and no bacteria as control for first factor, and beet cultivars including Pars, Torbat and line 31782 as second factor. Germination percentage affected with cultivar, bio-priming and their interaction, but germination rate was only affected by bio-priming. The highest germination percentage and was related to Pseudomonas putida and Pars cultivar. Application of bacteria increased germination rate versus control. Other mean comparison showed that Pseudomonas fluorescens increased root length, shoot length and seedling length and Pars and Torbat cultivars has more root and shoot length rather than Line 31782. Bio-prime with Pseudomonas fluorescens and Pseudomonas putida enhanced Vigor Index I, but Vigor Index II was only affected by cultivar. Pars and Torbat cultivar had higher germination traits and Vigor Index I and II to Line 31782 and showed more reactions to treatments. Generally, Bio-primingtreatments affected sugar beet cultivars versus control. Both bacterial strain act identically but Pseudomonas putida had more effects on cv. Pars.

Keywords


Ahmad, F., I. Ahmad, and M.S. Khan. 2005. Indole acetic acid production by the indigenous isolates of Azotobacter and Pseudomonas fluorescent in the presence and absence of tryptophan. Turkish J. Biol. 29:29–34.
Ahmad, F., I. Ahmad, and M.S. Khan. 2006. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol. Res. 36:1-9, 2006.
Akazawa, T., and I. Hara-Mishimura. 1985. Topographic aspects of biosynthesis, extracellular section and intracellular storage of proteins in plant cells. Annu. Rev. Phytopathol. 70: 441-472.
Baset Mia, M.A., Z.H. Shams Uddin, and Z. Wahab. 2010. Marziah M. Effect of plant growth promoting rhizobacterial (PGPR) inoculation on growth and nitrogen incorporation of tissue-cultured musa plantlets under nitrogen-free hydroponics condition. Aust. J. Crop Sci. 4(2):85–90,
Bashan, Y., G. Holguin, and L.E. de-Bashan. 2004. Azospirillum- plant relationships: physiological, molecular, agricultural, and environmental advances. Can. J. Microbial. 50: 521–577.
Bennett, A., and J. Whipps.2008. Beneficial microorganism survival on seed, roots and in rhizosphere soil following application to seed during drum priming. Biol. Control. 44:349–361.
Cakmakci, R., M. Erat, U.G. Erdoman, and M.F. Donmez. 2007. The influence of PGPR on growth parameters, antioxidant and pentose phosphate oxidative cycle enzymes in wheat and spinach plants. J. Plant Nutr. Soil Sci. 170: 288-295.
Cattelan, A.J., P.G. Hartel, and J.J. Fuhrmann. 1999. Screening for plant growth-promoting rhizobacteria to promote early soybean growth. Soil Sci. Soc. Am. J. 63: 1670–1680.
De Freitas, J.R., and J.J. Germida. 1992. Growth promotion of winter wheat by fluorescent pseudomonads under growth chamber conditions. Soil Biol. Biochem. 24: 1127-1135.
Egamberdiyeva, D. 2007. The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils. Appl. Soil Ecol. 36: 184-189.
Ehteshami, S.M.R., P. Tusi, Z. Amindeldar, and K Khavazi. 2010. Effect of seed inoculation with promoting growth bacteria in germination and growth of Brassica napus L. under different level of salinity. 1th National Oilseed Plants Conference. (In Persian with English Abstracts)
Frommel, M.I., J. Nowak, and G. Lazarovits. 1993. Treatment of potato tubers with a growth promoting Pseudomonas sp.: Plant growth responses and bacterium distribution in the rhizosphere. Plant Soil. 150 (1): 51- 60.
Gholami, A., S. Shahsavani, and S. Nezarat. 2009. The Effect of plant growth promoting rhizobacteria (PGPR) on germination, seedling growth and yield of maize. Proceedings of Word Academy of Science. Int. J. Agr. Biol. Eng. 37: 2070-3740.
Glick, B.R., L. Changping, G. Sibdas, and E.B. Dumbroff. 1997. Early development of canola seedlings in the presence of the plant growth promoting rhizobacterium Pseudomonas putida GR12-2. Soil Biol. Biochem. 29: 1233-1239.
Govindasamy, V., M. Senthilkumar, K. Gaikwad, and K. Annapurna. 2008. Isolation and characterization of ACC deaminase gene from two plant growth-promoting rhizobacteria. Curr. Microbiol. 57(4):312–317.
Haas, D., and C. Keel. 2003. Regulation of antibiotic production in root-colonizing Pseudomonas spp. and relevance for biological control of plant disease. Annu. Rev. Phytopathol. 41: 117–153.
Ikic, I., M. Maric evic, S. Tomasovic, J. Gunjaca, Z.S. Atovic, and H.S. Arcevic. 2012. The effect of germination temperature on seed dormancy in Croatian-grown winter wheat. Euphytica. 188:25-34.
International Seed Testing Association. 2011. International Rules for Seed Testing. International Seed Testing Association, Bassersdorf, Switzerland.
Karsa, K. K., and B. Abebie. 2012. Influence of seed priming on seed germination and vigor traits of Vicia villosa ssp. dasycarpa (Ten.). Afr. J. Agric. Res. 21:3202-3208.
Mia, M.A. B., Z.H. Shams Uddin, W. Zakaria, and M. Mariah. 2009. The effect of rhizobacterial inoculation on growth and nutrient accumulation of tissue-cultured banana plantlets under low N-fertilizer regime. Afr. J. Biotechnol. 8: 5855-5866.
Minorsky, P.V., 2008. On the inside. Plant Physiol. 146:323–324.
Moeinzadeh,A., F. Sharif-Zadeh, M. Ahmadzadeh,and F. Heidari Tajabadi. 2010. Biopriming of sunflower (Helianthus annus L.) seed with Pseudomonas fluorescens for improvement of seed invigoration and seedling growth. Aust. J. Crop Sci. 4(7): 564-570.
Nadjafi, F.2002. Effect of irrigation intervals and plant density on quantity and quality of Isubgol (Plantago ovate Forsk). M.Sc.Thesis: 45-52.
Noumavo, P.A., E. Kochoni, Y.O. Didagbe, A. Adjanohoun, M. Allagbe, M. Sikirou, E.W. Gachomo, S.O. Kotchoni, and L. Baba-Moussa. 2013. Effect of different plant growth promoting rhizobacteria on maize seed germination and seedling development. AJPS. 4: 1013-1021.
Pal, S.S. 1998. Interaction of an acid tolerant strain of phosphate solubilizing bacteria with a few acid tolerant crops. Plant Soil. 198: 169-177.
Patten, C.L., and B.R. Glick. 2002. The role of bacterial indole acetic acid in the development of the host plant root system. Appl. Environ. Microb. 68: 3795–3801.
Peirdashtie, H., H. hosseini, H. Firuzie, and P. Moradi. 2013. Study of some seed germination parameters of phaseolus vuigaris (green beans) under pretreatment with PGPR. 12th Agronomy and Breeding Congress of Iran. 14 to 16 septemer. Islamic Azad University of Karaj. (In Persian with English Abstracts)
Ribaudo, C., E. Krumpholz, F. Cassan, R. Bottini, M. Cantore, and A. Cura. 2006. Azospirillum sp. promotes root hair development in tomato plants through a mechanism that involves ethylene. J. Plant Growth Regul. 24:175–185.
Sahin, F., R. Cakmakci, and F. Kantar. 2004. Sugar beet and barley yields in relation to inoculation with N2- fixing and phosphate solubilizing bacteria. Plant Soil. 265: 123-129.
Salantur, A., A. Ozturk, and S. Akten. 2006. Growth and yield response of spring wheat (Triticum aestivum L.) to inoculation with rhizobacteria. Plant Soil Environ.52(3):111–118.
Sgroy, V., F. Cassan, O. Masciarelli, and M.F. Del Papa. 2009. Lagares A, Luna V. Isolation and characterization of endophytic plant growth-promoting (PGPB) or stress homeostasis-regulating (PSHB) bacteria associated to the halophyte Prosopis strombulifera. Appl. Microbiol. Biotechnol.85:371–381.
Shaukat, K., S. Affrasayab, and S. Hasnain. 2006. Growth responses of Helianthus annus to plant growth promoting rhizobacteria used as a biofertilizer. J. Agric. Res. 1 (6): 573-581
Werner, T., V. Motyka, V. Laucou, R. Smets, H.V. Onckelen, and T. Schmulling. 2003. Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity. Plant Cell. 15:2532–2550.
Wu, S.C., Z.H. Cao, Z.G. Li, K.C. Cheung, and M.H. Wong. 2005. Effects of biofertilizer containing 
N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma. 125: 155–166.
Yadav, J., J.P. Verma, and K.N. Tiwari. 2010. Effect of Plant Growth Promoting Rhizobacteria on Seed Germination and Plant Growth Chickpea (Cicer arietinum L.) under in Vitro Conditions. Biol. Forum Int. J. 2: 15-18.