Studying interaction of Moist-Chilling and gibberellic acid on germination of Fritillaria imperialis

Document Type : Original Article

Authors

1 MSc. Of seed science and technology/Shahrekord Univ., Shahrekord, Iran.

2 Faculty member/ Shahrekord University, Shahrekord, Iran.

Abstract

There are several limitations in reproduction of Fritilaria imperilais using tissue tissue culture, lamination and bulb division. In this study, we designed a factorial experiment in a randomized complete block for evaluating the effects of several treatments such as cold duration (0, 4 and 8 weeks), concentrations of GA3 (0, 250 and 500 ppm) and application time in three steps (before, during and after stratification). The results showed that the seeds were highly germinated using 8 weeks stratification compares to others. Moreover, 500ppm gibberllin before stratifications significantly increased percentage of germination, Vigor Index Ι, length of radical, plumule, seedling and seedling dry weight, however significantly decreased E50. The results also showed that increasing the GA3 from 250 to 500ppm had significantly positive effect on all the traits. This study indicates that 8 weeks stratification along with 500ppm is more likely to increase the germination index of Fritilaria imperilais.

Keywords

References

Amooaghaie, R., 2007. The Effect of Moist-Chilling and GA3 on seed dormancy breaking of Ferula ovina Boiss. JWSS. 11(40): 471-481. (In Persian, with English abstract).
Baskin, C.C., J.M. Baskin, and E.W. Chester, 1999. Seed dormancy in the wetland winter annual Ptilimnium nuttalli (Apiaceae). The Society of Wetland Scientists, 19(2): 359-364.
Bentsink, L., and M. Koornneef. 2002. Seed dormancy and germination. The Arabidopsis Book 1-18.
Bewley J.D. 1997. Seed Germination and Dormancy. Plant Cell. 9:1055–1066.
Bradford, K.J., and H. Nonogaki. 2007. Seed Development, Dormancy and Germination. Wiley-Blackwell. Oxford.
Copeland, L.O., and M.B. Mcdonald. 2001. Seed Science and Technology. Springer Science+Business Media, Llc, New York.
Dashti, F., H. Ghahremani- Majd, and M. Esna-Ashari. 2012. Overcoming seed dormancy of mooseer (Allium hirtifolium) through cold stratification, gibberellic acid, and acid scarification. J. Forestry Res. 23(4):707-710.
De Hertogh, A. 1990. Basic criteria for selecting flower bulbs for North American markets Gardens, Outdoor cut flowers, forced cut flowers and potted plant. North Carolina Horticoltuyal Research series Number 85, Raliegh.
De Hertogh, A., and M. Le Nard. 1993. The Physiology of flower bulbs. Elsevier, the University of Michigan.
Dhoran V.S., and S.P. Gudadhe. 2012. Effect of plant growth regulators on seed germination and seedling vigour in Asparagus sprengeri Regelin. Int. Res. J. Biol.1 (7):6-10.
Draghici, C., and I.V. Abrudan. 2010. Dormancy Breaking Of Acer and Fraxinus Seeds -A Brief Review. Bull. Transilvania Univ. Brasov, 3(52): 29-32.
Ebrahimi R., 1M. Hassandokht, Z. Zamani1, A. Kashi1, I. Roldan-Ruiz, and E.V. Bockstaele. 2014. Seed Morphogenesis and Effect of Pretreatments on Seed Germination of Persian Shallot (Allium hirtifolium Boiss.), an Endangered Medicinal plant.  Hortic. Environ. Biotechnol. 55(1):19-26.
El-Dengawy, E.F.A. 2005. Promotion of seed germination and subsequent seedling growth of loquat (Eriobotrya japonica, Lindl) by moist-chilling and GA3 applications. Sci. Hortic, 105: 331–342.
Gao, S.L., D.N. Zhu, Z.H. Cai, Y. Jiang, and D.R. Xu. 1999. Organ culture of a precious Chinese medicinal plant – Fritillaria unibracteata. Plant Cell Tiss. Org. 59: 197–201.
Kabar, K., 1998. Comparative Effects of Kinetin, Benzyladenine, and Gibberellic Acid on Abscisic Acid Inhibited Seed Germination and Seedling Growth of Red Pine and Arbor Vitae.  Turkish J. Bot. 22: 1-6.
Karimi, H. 2009.Atlas of medicinal plants. Abnous Publisher, Tehran. (In Persian).
Kleynhans, R., and J.J. Spies. 2011. Requirements for the development and breeding of new flower bulb crops. Philos. T. Genetics, 1: 80-101.
Lin, G., P. Li,  S.L. Li, and S.W. Chan. 2001. Chromatographic analysis of Fritillaria isosteroidal alkaloids, the active ingredients of Beimu, the antitussive traditional Chinese medicinal herb. J. Chromatoger. A. 935: 321-328.
Mancuso, E., G. Bedini, and L. Peruzzi. 2012. Morphology, germination, and storage behaviour in seeds of Tuscan populations of Fritillaria montana (Liliaceae), a rare perennial geophyte in Italy. Turkish J. Bot.36: 161-166.
Meamr-Moshrefi, M. 1998. Effect of plant growth regulators and environmental condition on growth, development, propagation of bulb and scenesence of flower of Fritillria (Fritillaria imperialis). Ph.D. Thesis. Univ. of Tarbiat Modares, Tehran.(In Persian).
Mohammadi-Dehcheshmeh, M., A. Khalighi, R. Naderi, M. Sardari, and E. Ebrahimie. 2008. Petal: a reliable explant for direct bulblet regeneration of endangered wild populations of Fritillaria imperialis L. Acta Physiol. Plant. 30: 395-399.
Nabaei, M., P. Roshandel, and A. Mohammadkhani. 2011. Effective techniques to break seed dormancy and stimulate seed germination in (Rheum ribes L.). Iranian J. Med. Aromat. Plant. 27(2): 212-223.
Nalawade, S.M., A.P. Sagare, Ch.Y. Lee, Ch.L. Kao, and H.Sh. Tsay. 2003. Studies on tissue culture of Chinese medicinal plant resources in Taiwan and their sustainable utilization. Bot. Bull. Acad. Sin, 44: 79-98.
Rahimi, M., M.H. Daneshvarm, M. Heidari, and F. Yari. 2013. In vitro micropropagation of Fritillaria imperialis L. through induction of indirect organogenesis. Int. J. Agron. Plant Prod. 4: 418-424.
Rahnama-Ghahfarokhi, A., and R. Tavakol-afshari. 2007. Methods for dormancy breaking and germination of Galbanum seeds (Ferula gummosa). Asian J. Plant Sci. 6(4): 611-616.
Rahman, A.U., M.N. Akhtar, M. Choudhary. Y. I.q. Tsuda, B. Sener, A. Khalid, and M. Parvez. 2002. New steroidal alkaloids from Fritillaria imperialis and their cholinesterase inhibiting activities. Chem. Pharm. Bull. 50: 1013-1016.
Ronsted, N.S., H. Law Thornton, M.F. Fay, and M.W. Chase. 2005. Molecular phylogenetic evidence for the monophyly of Fritillaria and Lilium (Liliaceae, Liliales) and the infrageneric classification of Fritillaria. Mol. Phylogenet. Evol. 35: 509-527.
Rood, S.B., R.L. Buzzell, D.J. Major, and R.P. Pharis. 1990. Gibberellins and Heterosis in Maize: Quantitative Relationships. Crop Sci. 30:281-286.
Rouhi, H.R., K. Shakarami, and R. Tavakkol Afshari. 2010. Seed treatments to overcome dormancy of waterlily tulip (Tulipa kaufmanniana Regel.). Aust. J. Crop Sci. 4(9): 718-721.
Vandelook, F., N. Bolle, and J.A. Van Assche. 2007. Seed Dormancy and Germination of the European Chaerophyllum temulum (Apiaceae), a Member of a Trans-Atlantic Genus. Ann. Bot, 100: 233–239.
Wang, sh., W.Gaoa, H. Chena, and P. Xiaob. 2004. New starches from Fritillaria species medicinal plants. Carbohydr. Polym. 61(1): 111–114.
 
Iranian Journal of Seed Science and Technology
Volume 6, Issue 2 - Serial Number 12
Autumn and Winter of 2017-18
November 2017
Pages 257-266

Files

Share

How to cite

Statistics