Document Type : Original Article

Abstract

One of the major factors for the low productivity of seeds is poor seed germination and early seedling mortality due to seed-borne fungi. Several grain-borne fungi, including species of Alternaria, Aspergillus, Fusarium and Penicillium, have been implicated as seedling pathogens of cereals. Experiments were conducted at the seed and Plant certification and Registration Research Institute. Experimental units were arranged factorial in a completely randomized design with two factors (cultivar and storage temperature). The result indicated that interaction between storage temperature × cultivar had significantly affected on seed-borne fungi after three months storage. The seed-borne fungi infestation sach as Asporgilau flavus and Penicillium decreased in all seeds that stored at 20-30C° (alternative temperature), also the Fusarium species infestation was reduced in Usef, Nosrat, and Productive Cultivars seed under storage at 20-30C°, whereas Alternaria alternate infestation was increased in all seeds that stored at 20-30 C° . Meanwhile, normal seedling percentage and seedling vigor were significantly improved by storage seeds at 20-30 C° that it was more pronounced in Sahra and Productive cultivars. Final germination percentage and normal seedling percentage increased, when seed-borne fungi infestation such as Aspergillus flavus,Penicillium sppand Fusarium spp decreased in seed samples. Generally, according to cultivars that examined in this experiment, seed stored at 20-30 C°(alternative temperature) could have maintained their vigour high, it is new founding that could help to control seed-borne fungi (Asporgilau flavus, Penicilliumsppand Fusarium spp) in storage condition also prevent of seed deterioration.

Keywords

Abdul-Baki, A.A. and Anderson, J.D.1973. Vigor Determination in Soybean Seed by Multiple Criteria. Crop Sci., 13:630-633.
Abramson,D., R.N. Sinha, and J.T. Mills.1983. Mycotoxin and odor formation in barley stored at 16 and 20% moisture in manitoba. Cereal Chem.5:350-355.1983.
Anonymous. 2011. International rules for seed testing edition. International Seed testing Association (ISTA).
Conrath, U., C.M.J. Pieterse, and B. Mauch-MANI. 2002. Priming in plant pathogen in teractions.Trends Plant Sci. 7: 210-216.
Hasan, M.M., S.P. Chowdhary, S.Alam, B. Hossain, and M.S. Alam. 2005. Antifungal effects of plant extracts on seed-borne fungi of wheat seed regarding seed germination, Seedling Health and Vigaur Index, Pak. J. Biol. Sci.8:1284-1289.
Hassan, H.A.H. 1999. Phytotoxicity of pathogenic fungi and their mycotoxins to cereal seedling viability. Mycopathologia. 148:149-155.
Hudec, K. 2007. Infuse of harvest date and geographical location on kernel symptoms, fungal infestation and embryo viability of malting barley. Int. J. Food Microbiol.113:125-132.
Jorgensen, J. 1970. Changes in the fungus flora of barley seed stored with a high moisture content. Tidsskrift for planteavl. 74:425-432.
Karunakaran, C., W.E. Muir, D.S. Jayas, N.D.G. White, and D. Abramson. 2001. Safe storage time of high moisture wheat. J. Stored Prod. Res. 37: 303-312.
Karunakaran, C. 1993. Modelling safe storage time of high (17 and 19%) moisture content wheat. FGS [Online]. Electronic Theses & Dissertations (public). Available at Http://hdl.handle.net/1993/1575.
Mathre, D.E. 1997. Compendium of Barley Disease, Second edition. APS Press, St.Paul.ISBN:0-89054-180-9.
Kosiak, B. M. Torp, E. Skjerve, and B. Andersen. 2004. Alternaria and Fusarium in Norwegian grains of reduced quality-a matched pair sample study. Int J Food Microbiol.93: 51-62.
Mathur, S.B. and, O. Kongsdal. 2003. Common laboratory seed Health testing methods for detecting fungi. International Seed Testing Association (ISTA).
Pathak, N. and, R.K. Zaidi. 2013. Studies on seed-borne fungi of wheat in seed health testing programme. Arch. Phytopathol. Plant Protect. 46: 389-401.
Reuss, R., J. Cassells, and R. Nischwitz. 2002. Storage conditions affect the quality of malting barley.CSIRO Stored Grain Research Laboratory,Canberra, ACT 2601, Australia.
Saric,M., M. Skrinjar, G. Dimic, N. Filipovic, and J. Rasic. 1997. Changes in hygienic and technological wheat quality caused by mould infection. Acta Alimentaria. 26:255-269.
Scott, S.J., R.A. Jones, and W.A. Williams. 1984. Review of data analysis methods for seed germination. Crop Sci. 24:1192-1198.
Zare, L. 2012. Certified barley seed contamination with seedborne diseases. J. Appl. Sci. Eng. Technol. 2(S): 530-534.