The effect of gibberellic acid and potassium nitrate on seed dormancy of fourteen medicinal plant species ( Lamiaceae)

Document Type : Original Article

Authors

1 student

2 Supervisor of Faculty of Agriculture, Ferdowsi University of Mashhad.

3 Professor of Department of Crop Science, Faculty of Agriculture, Ferdowsi University of Mashhad

4 Department of Natural Resources, Kerman Agricultural and Natural Resources Research Center, AREEO, Kerman, Iran

Abstract

To study the effect of gibberellic acid and potassium nitrate treatments on germination and seed dormancy of 14 medicinal plant species from the Lamiaceae, that were collected in the spring and summer of 1392 from their natural habitates in Kerman province, two separate laboratory experiments were conducted at 20-24 centigrade degree, based on completely randomized design with 4 replications of 25 seeds. In the first experiment, seed germination was considered in H2O. The results indicated that the highest germination percentages were observed in Ocimum basilicum (%96) and Salvia macrosiphon (%95) while the lowest germination were obtained in Marrubium crassidens (%5) and Nepeta bracteata (%0). In the second experiment, the effect of seed dormancy breaking treatments such as gibberellic acid (GA3) with 3 concentrations (250, 500 and 1000 ppm) and potassium nitrate with 2 concentrations (500 and 1000 ppm) were practiced on the germination of 10 plant species with low germination (below %80) in H2O. Dormancy breaking treatments were effective on 9 species (i.e. Marrubium crassidens and Ocimum santum) out of 10 species. In most studied species, dormancy breaking treatments increased germination percentage and Mean Germination Time were reduced. Therefore, based on their reactions to the treatments, it was found that these 9 species have physiological dormancy.

Keywords


Amoaghaei, R. 2007. The effect of GA3 and moist chilling on seed dormancy breaking of Ferula ovina Boiss. Scie. Tech. Agric. Nat. Res. 11 (40 (b)): 471-481. (In Persian).
Atul, S., and N.R. Shiresh Sharma. 2000. Standardized cultivation method for viola species: an AIDS curing agent. J. Trop. Med. Plants. 1:109-114.
Baskin, C.C., and J. M., Baskin.1998. Types of Seed Dormancy. Academic Press, Harcourt Brace and Company.
Bewley, J. D., and M. Black. 1994. Seeds: Physiology of Development and Germination, (2nd Ed.) Plenum Press, New York.
Bodeker, G., k. k. S. Baht, J. Burley, and P. Vantomm. 1997. Medicinal Plants for forest conservation and health care. Non-wood Forest Products 11: FAO, Rome.
Chakraborty, D. K. Bhattacharya, A. Bandyopadhyay, and K. Gupta. 2003. Studies on the germination behavior of Basilicum polystachyon- an ethnobotanically important medicinal plant. J. Med. Aromat. Plants 25: 58-62.
Chuanren, D., W. Bochu, L. Wanqian, C. Jing, L. Jie, and Z. Huan. 2004. Effect of chemical and physical factors to improve the germination rate of Echinacea angustifolia seeds. Colloids Surf. B: Biointerfaces. 37:101-105.
Copland, L. O., and M. B. Mc Donald. 1995. Principles of Seed Sience and Technology. A. Ghaderi, A. Kamkar, and A. Soltani (Translators), 2011. Jahad publications (Mashhad) (In Persian).
Ehiaei, H. A., and M. Khajeh-Hosseini. 2012. Evaluation of germination characteristics and dormancy of thirty species of medicinal plants. Iranian J. Field Crops Res. 9 (4): 651-658. (In Persian).
Ghadamyari, Sh., J. Mozafari, N. Sokhandan Bashir, L. Mosavi, and F. Rakhshanderoo. 2011. Synergistic effects of mechanical and chemical treatments on seed germination of Jimson weed (Datura stramonium L.). Iranian J. Biol. 24 (6): 809-817. (In Persian, with English Abstract).
Ghasemi Pirbalooti, A., A. R. Golparvar, M. Riahi Dehkordi, and A. Navid. 2005. Investigation of the effects of different treatments, on breaking dormancy and germination of medicinal plant seed of Thymus daenesis Celak. Iranian J. Medic. Aromat. Plants. 21 (3): 371-379. (In Persian, with English Abstract).
Giba, Z., D., Grubisic, S. Todorovic, L. Sajc, D. Stojakovic, and R. Konjevic. 1998. Effects of Nitrate-Oxide releasing compounds on phytochrome-controlled germination of empress tree seeds. Plant Growth Regul. 26(2): 175-181.
Gupta, V. 2003. Seed germination and dormancy breaking techniques for indigenous medicinal and aromatic plants. J. Med. Aromat. Plants. 25: 402-407.
Harnischfeger, G. 2000. Proposed guidelines of commercial collection of medicinal plant material. J. Herbs Spices Med. Plants. 7(1): 43-50.
Hosseinpour Gazviniy, A.A., M.A. Alizadeh, A.A. Jafari, and A.R.Valadabadi. 2011. Effect of scarification, cold and after-ripening treatments on seed dormancy breaking in four species of Satureja by standard germination test. Iranian J. Med. Aromat. Plants. 28 (1): 48-58. (In Persian, with English Abstract).
International Seed Testing Association. 1979. The germination test. Seed Sci. Technol. 4: 23-28.
Khajeh-Hosseini, M., A. Lomhololt, and S. Matthews. 2009. Mean germination in the laboratory estimates the relative vigor and field performance of commercial seeds lots of maize (Zea mays L.). Seed Sci.Technol. 37: 446-456.
Khajeh-Hosseini, M., and T.  Mostashar-Shahidi. 2016. Role of mucilage in germination of fourteen species of medicinal plants. Seed Sci. Technol. 44(2): 435-440.
Koocheki, A., and G. Azizi. 2005. Effect of different treatments on breaking dormancy of Teucrium polium. Iranian J. Field Crops Res. 3(1): 81-87. (In Persian).
Makkizadeh Tafti, M., R. Farhoudi, H.A. Naghdibadi, and A. Mehdizadeh. 2006. Assigning the best treatment for increasing germination of three medicinal plants seeds: Rubia tinctourum L., Echinaceae angustifolia D. C. and Myrtus communis L. Iranian J. Med. Aromat. Plants. 22 (2): 105-116.
Makkizadeh Tafti, M., A. Farhoudi, M.A. Rastifar, and K. Sadat Asilan. 2012. Methods of breaking dormancy in Caper (Capparis spinosa L.). Iranian J.  Range Desert Res. 18 (4): 569-577. (In Persian, with English Abstract).
Nabaei, M., P. Roshandel, and A. Mohammad Khani. 2011. Effective techniques to break seed dormancy and stimulate seed germination in Rheum ribes L. Iranian J. Med. Aromat. Plants. 27 (2): 212-223. (In Persian, with English Abstract).
Nadjafi, F., A. Koocheki, P. Rezvani Moghaddam, and M. Rastgoo. 2006. Evaluation of seed germination characteristics in Nepeta binaludensis, a highly endangered medicinal plant of Iran. Iranian J. Field Crops. 4 (2): 1-8. (In Persian).
Lambert, J., J. Sirvastava, and N. Vietmeyer. 1997. Medicinal plants. Rescuing a global heritage.Washington DC, World Bank Technical Paper. No. 355.
Lange, D. 1998. Europ’s Medicinal and Aromatic Plants: Their Use, Trade and Conservation. Traffic Europe/ International, Cambridge UK.
Nikolaeva, M. G. 1977. Factors controlling, the seed dormancy patterns. Pp 51-74. In: A.A. Khan (Ed). The Physiology and Biochemistry of Seed development, Dormancy and Germination. Elsevier/North-Holland, Amsterdam.
Sari, A. O., M. R., Morales, and J. E., Simon. 1999. Echinaceae angustifolia: An Emerging Medicinal Food. pp: 490-493. In: J. Janick (Ed.). Perspectives on new crops and new uses.ASHS Press, Alexandria, V. A.
Shariati, M., T. Asemaneh, and M. Modares Hashemi. 2002. Investigation the effects of different treatments on Dormancy breaking of Achilea millefollum seeds. Q. Pajouhesh Va-Sazandegi. 56 and 58: 2-8. (In Persian).
Tieu, A., K.W. Dixon, K.A. Meney, K., Sivasithamparam, and R.L. Barrett. 2001. Spatial and developmental variation in seed dormancy characteristics in the fire-responsive species Anigozanthos manglessi (Haemodoraceae) from Western Australia. Ann.  Bot. 88: 19-26.
Uniyal, R. C., M. R., Uniyal, and P. Jain. 2002. Cultivation of medicinal plants in India: a reference book. New Delhi, India, TRAFFIC India & WWF India.
WHO, IUCN and WWF. 1993. Guidelines on the Conservation of Medicinal Plants. Geneva, Switzerland.