Document Type : Original Article

Authors

• Mehdi Kakaei ¹
• Mohsen Mansouri ²

¹ Assistant Prifessor-PNU

² Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Abstract

In this study of Nigella sativa seeds Cryopreservation by vitrification. was studied. This experiment with three replications with two factors, both soluble dewatering (Pvs2 and Pvs3) times and dewatering of seeds before freezing process during the 7 period (20, 40, 60, 80, 100, 120 and 140 minutes) with factorial arrangement in a completely randomized design (CRD) was analyzed. Results showed that treatment time for root length, shoot length was significant at the level of 1%, but the percentage of germination (percent survival) was non-significant. The solution for all traits (germination, root and shoot length) at 1 percent difference was significant. The interaction between the treatment solution for the length of root and shoot length at 1 percent and germination percentage was significant at the 5% level. Overall, analysis of variance of the solution and dewatering time in the process of vitrification and freeze protection Nigella sativa is effective in assessment indicators. Overall, Analysis of variance showed that the soluble kind and the dewatering process and vitrification Cryopreservation of Nigella sativa on effective evaluation indicators. Also, seed treatment with solution PVS3 and during intervals 20 and 40 minutes, was the highest amount of root length, shoot length and germination. The results of this study can be used in medicinal plant germplasm conservation and the importance of effective treatment.

Keywords

• Nigella sativa
• Cryopreservation
• Canserve solution
• vitrification