Document Type : Original Article
Authors
Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil , Iran.
Abstract
In order to study the effects of plant growth promoting rhizobacteria (PGPR) and salinity stress on seed germination parameters, K and Na content of rye (Secale cereal L.) seedling , two factorial experiments based on CRD and RCB design were conducted with three replications at laboratory and greenhouse respectively at faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabil in 2016. Experimental factors were included soil salinity in four levels (no-salt as control, salinity 25, 50 and 75 mM as NaCl) and seed inoculation with plant growth promoting rhizobacteria in four levels (no inoculation as control, inoculation with Pseudomonas, Azosprilium, both application of Pseudomonas and Azosprilium). The results showed that maximum germination components (such as ridicule and plumule length, radicule and plumule dry weight, germination percentage, uniformity of germination) were obtained at co-application of Pseudomonas and Azosprilium under non-saline condition and minimum of these parameters were obtained at 75 Mm NaCl and no inoculation. K/Na ratio in root and shoot were decreased with increasing salinity level. It was vise versa in seed inoculation with plant growth promoting rhizobacteria. Maximum ratio of Na/K was obtained at the highest level salinity and no inoculation seed with PGPR and minimum of it was obtained in both inoculation with Pseudomonas and Azosprilium and the least level of salinity. It seems that application of PGPR can be used as a proper method for increasing K /Na ratio, germination components and seedling growth of rye under salinity stress.
Keywords
Alamgir, A., K.K. Kutube, and T. Paul. 1997. Use of mathematical growth curves in the analaysis of growth and nutrient distribution pattern in wheat growth under salinity stress. Agron. J. 21:37-46.39.
Araghi Shahri, S.M., G.A. Dianati Tilaki, B. Behtari, and M.A Alizadeh. 2015. Growth responses of Secale cereale and S.ceremont to priming treatments under salinity stress. J. Rangeland Sci. 5(3): 202-211. (In Persian, with English Abstract)
Ashraf, M. 2004. Some important physiological selection criteria for salt tolerance in plant. Flora. 199:361-376.
Ashraf, M, and T. McNielly.2004. Salinity tolerance in Brassica oil seeds. Crit. Rev. Plant. 34: 34-45.
Ashraf, M, and M.R. Foolad, 2005. Pre-sowing seed treatment shotgun approach to improve germination, plant growth, and crop yield under saline and non-saline conditions. Adv. Agron. 88: 223–271.
Bacilio, M., H. Rodriguez, M. Mereno, and J.P. Hernandez. 2004. Mitigation of salt stress in wheat seedling by Azospirillum lipferum. Soil Biol. 40: 188-193.
Baset, M.A., Z.H. Shamsuddin, and M. Maziah.2010. Use of plant growth promoting bacteria in banana. A new insight sustainable banana production. Int. J. Agric. Biol.12:459-467.
Bashan, Y., Y.H. Ivanony, and A. Saad. 1989. Non specific response in plant growth, yield and root colonization of non-cereal crop plant to inoculation with Azospirilum brasilense. Can. J. Bot. 67:1317-1324
Benlloch, M., M.A. Ojeda, J. Ramos, and A. Rodriguesnanavarro. 1994. Salt sensitivity and low discrimiation between potassiumand and sodium in bean plants. Plant and Soil. 166: 117-123.
Bharathi, R., R. Vivekananthan, S. Harish, A. Ramanathan, and R. Samiyappan. 2004. Rhizobacteria-based bio-formulations for the management of fruit rot infection in hillies. Crop Prot. 23: 835–843.
Bhatt, R.M, and N.K.Srinavasa Rao.1987. Seed germination and seedling growth responses of tomato cultivars to imposed water stress. J. Hort. Sci. 62: 221-225.
Cakmakı, R., F. Kantar, and F. Fiahin. 2001. Effect of N2-fixing bacterial inoculations on yield of sugar beet and barley. J.Plant Nutr. Soil Sci. 164: 527-31.
Cakmakci, R., M. Erat, U.G. Erdoman, and M.F. Donmez. 2007. The influence of PGPR on growth parameters, antioxidant and pentose phosphate oxidative cycle enzymes in wheat and spinach plants. J. Plant Nutr. Soil Sci. 170: 288-295.
Chen, Z., I. Newman, M. Zhuo, N. Mendham, G. Zhang, and S. Shabala. 2005. Screening plant for salt tolerance by measuring K+ flux: a case study for barely. Plant, Cell and Environ. 28: 1230-1246.
De, R, and R.K. Kar. 1995. Seed germination and seedling growth of mung bean (Vigna radiata) under water stress induced by PEG 6000. Seed Sci. Technol. 23: 301-308.
Dobbelaere, S., J.Vanderleyden, and Y.YacovOkon. 2003. Plant growth-promoting effects of diazotrophs in the rhizosphere. Crit. Rev. Plant Sci. 22: 107-149.
El-Keblawy, A, and A. Al-Rawai. 2005. Effects of salinity, temperature and light on germination of invasive Prosopis juliflora. J. Arid Environ. 61: 555-565.
Garcia, A., C.A. Rizzo, J.UD-Din, S.L. Bartos, T.J. Flowers, and A.R. Yeo. 1997. Sodium and potassium transpott to the xylem are inherited independent lyinrice, and the mechanisms of sodium: potassium selectivity differs between rice and wheat. Plant Cell and Enviroment. 20: 1167-1174.
Gholami, A., S.Shahsavani, and S. Nezarat. 2009. The Effect of plant growth promoting rhizobacteria (PGPR) on germination, seedling growth and yield of maize. Proceedings of Word Academy of Science. Engineering and Technology. 37: 2070-3740. (In Persian)
Gilick, B.E., D.Penrose, and M. Wenbo. 2001. Bacterial promotion of plant growth. Biotechnol. J. Adv. 19: 135-138.
Glick, B.R. 1998. A model for the lowering of plant ethylene concentration by PGPR. J. Theor. Biol. 190: 63-68.
Hamdia, M.A, and H.M. El-Komy.1997. Effects of salinity, gibberelic acid and Azosbirillum inoculation on growth and nitrogen uptake of Zea mays. Plant Biol. 40:109-120.
Hernandez, A.N., A. Hernandez, and M. Heydrich. 1995. Selection of rhizobacteria for use in maize cultivation. J. Trop. Sci. 6: 5-8.
Kapulnik, Y., S. Sarig, A. Nur, Y. Okon, and Y. Henis.1982. The effect of Azospirillum inoculation on growth and yield of corn. J. Bot. 31: 247-255.
Karlidag, H., A. Esitken, E.Yildirim, M. Figen-Donmez, and M. Turan. 2011. Effects of plant growth promoting bacteria on yield, growth, leaf water content, membrane permeability and ionic composition of strawberry under saline conditions. J. Plant Nutr. 23:157-174.
Khan, M.R., N.C. Talukdar, and D. Thakuria. 2003. Detection of Azospirillum and PSB in rice rhizosphere soil by protein and antibiotic resistance profile and their effect on grain yield of rice. Ind. J. Biotechnol. 2: 246-250.
Kheirizadeh Arough, Y., R. Seyed Sharifi, M. Sedghi, and M. Barmaki. 2016. Effect of zinc and bio fertilizers on antioxidant enzymes activity, chlorophyll content, soluble sugars and proline in Triticale under salinity condition. J. Not. Bot. Hort. Agro Cluj-Napoca. 44(1):116-124.
Lucy, M., E. Reed, and B.R.Glick. 2004. Applications of free living plant growth-promoting rhizobacteria. Soil Sci. 86:1-25.
Lynch, J, and A. Lauchli. 1988. Salinity affects intercellular calcium in corn root protoplasts. Plant Physiol. 87: 351-356.
Mayak, S., T. Tirosh, and B.R. Glick. 2004. Plant growth promoting bacteria confer resistance in tomato plants salt stress. Plant Physiol. Biochem. 42: 565-572.
Meyer, S.E, and R.L. Pendleton. 2000. Genetic regulation of seed dormancy in Purshia tridentata (Rosaceae). Ann. Bot. 85: 521-529.
Nadeem,S.M., Z.A. Zahir, M. Naveed,and M. Arshad. 2007. Preliminary investigations on inducing salt tolerance in maize through inoculation with rhizobacteria containing ACC deaminase activity. Can. J. Microbiol. 53 (10):1141-9.
Pal, S.S. 1998. Interaction of an acid tolerant strain of phosphate solubilizing bacteria with a few acid tolerant crops. Plant and Soil. 198: 169-177.
Prisco,J.T.,CR.Baptista,and J.L. Pinheiro Bastos. 1992. Effect of seed pre-treatment on seed germination under water stress conduction. Rev. Brasil. Bot. 15(1):31-35.
Rao, A.V, and B.V. Warla.1985. Salt tolerance of azospirillum brasilense. Acta Microbiol. 32: 221-224.
Sadeghian, S.Y, and N.Yavari. 2004. Effect of water –deficit stress on germination and early seedling growth in sugar beet. J. Agron. Crop Sci. 190(2):138-144. (In Persian, with English Abstract)
Sahin, F., R.Cakmakci, and F. Kantar. 2004. Sugar beet and barley yields in relation to inoculation with N2-fixing and phosphate solubilizing bacteria. Plant and Soil. 265: 123-129.
Sairam, R.K., K.Veerabharda, and G.C.Srivastsva. 2002. Differenalnresponce of wheat genotype to longh term salinity stress in relation to oxidative stress. Ontioxidant activity and osmolyte concentration. Plant Sci. 163:1037-104.
Salantur, A., A.Ozturk, and S. Akten. 2006. Growth and yield response of spring wheat (Triticum aestivum L.) to inoculation with rhizobacteria. Plant Soil. Environ. 52 (3):111–118.
Seyed Sharifi, R, and K.Khavazi. 2011. Effect of seed priming with plant growth promoting rhizobacteria (PGPR) on germination components and seedling growth of corn (Zea maize L.). Agroecol. J. 3(4): 560-570. (In Persian, with English Abstract)
Seyed Sharifi, R, and A. Namvar. 2016. Bio fertilizers in Agronomy. University of Mohaghegh Ardabili press, Ardabil. (In Persian)
Shaharoona, B.M., Z.Arshad, A.Zahir, and A. Khalid. 2006. Performance of Pseudomonas spp. containing ACC-deaminase for improving growth and yield of maize (Zea mays L.) in the presence of nitrogenous fertilizer. Soil Biol. Biochem. 38: 2971–2975.
Shaukat, K., S. Affrasayab, and S. Hasnain. 2006. Growth responses of Helianthus annus to plant growth promoting rhizobacteria used as a biofertilizer. J. Agric. Res. 1 (6): 573-581.
Soltai, A.S, E.Galeshi, Zenali, and N.Latif. 2001. Germination seed resevve utilization and growth of chickpea as affected by salinity and seed size. Seed Sci.Technol. 30: 51-60.
Soltani, A., M. Gholipoor, and E. Zeinali. 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environ. Exp. Bot. 55: 195-200.
Vessy, J.K. 2003. Plant growth promoting rhizobacteria as biofertilizer. Plant and Soil. 3:355-358.
Voigt, E.L., T.D. Almeida, R.M. Chagas, L.F.A. Ponte, R.A.Viégas, and J.A.G. Silveira, 2009. Source–sink regulation of cotyledonary reserve mobilization during cashew (Anacardium occidentale) seedling establishment under NaCl salinity. J. Plant Physiol. 166: 80–89.
Wagar, A., B.Shahroona, Z. Zahir, and M. Arshad. 2004. Inoculation with Acc deaminase containing rhizobacteria for improvming growth and yield of wheat. Pak. J. Agric. 41: 119-124.
Windauer, L., A. Altuna, and R. Benech-Arnold. 2007. Hydrotime analysis of Lesquerella fendleri. Crop Sci.32:256-262.
Wu, S. C., Z. H. Cao, Z.G. Li, K. C. Cheung, and M. H. Wong. 2005. Effects of bio fertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma. 125: 155-166.
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