Document Type : Original Article
Authors
1 , Faculty of Agriculture, the University of Shahrekord
2 Faculty of Basic Sciences, Shahrekord
3 Faculty of Agriculture, Shahrekord University
Abstract
In order to comparison the effects of growth promoting bacteria on germination of hamadani alfalfa seeds, a randomized complete block design was conducted with 4 replications and 8 levels of bacterial inoculation (include non-inoculation, application of Acinetrobacter calcoaceticus PTCC 1318, Bacillus megaterium PTCC 1250, Enterobacter aerogenes PTCC 1221 lonely and their pairwise and triple combination of them(. Another CRD factorial experiment was also conducted with 3 replications in greenhouse conditions. Mentioned bacterial treatments were as the first factor and injection of bacteria into the soil of pots as the second factor. The results showed that application of bacteria increased the vigor index of seedling, percentage and germination rate, but did not affect other alfalfa traits in the laboratory. Inoculation of seeds with bacteria in the pots had positive effects on alfalfa growth, percentage and rate of emergence, dry weight, root volume and length, chlorophyll a, b and total chlorophyll content. Injection of bacteria to the soil also affected some traits, which could be due to better placement and/or a larger population of bacteria in the rhizosphere. Application of bacteria lonely had better results than their combinations. It may be due to antagonistic effects of bacteria on each other, which may reduce positive effects.
Keywords
Abdul-baki, A.A., and J.D. Anderson. 1973. Vigor determination in soyabean by multiple criteria. Crop Sci. 10: 31-34.
Afrakhteh, S., E. Frahmandfar, A. Hamidi, and H.D. Ramandi. 2013. Evaluation of growth characteristics and seedling vigor in two cultivars of soybean dried under different temperature and fluidized bed dryer. Int. J. Agric. Crop Sci. 5(21): 2537-2544.
Ahmad, F., I. Ahmad, and M.S. Khan, 2008. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol. Res. 163:173-181.
Akhtar, M.J., S. Ullah, I. Ahmad, A. Rauf, S.M. Nadeem, M.Y. Khan, S. Hussain, and L. Bulgariu. 2017. Nickel phytoextraction through bacterial inoculation in Raphanus sativus. J. Res. Gate. 190: 234-242.
Alizadeh, A. 2010. Water, soil and plant relationships, Imam Reza University Press. Mashhad. pp 616. (In Persian)
Amooaghaie, R., and F. Nikandish. 2015. Effect of root inoculation of two alfalfa cultivars with strains of Bacillus and Sinorhizobium species on growth, chlorophyll content and cell membrane stability under salinity stress. J. Plant Res. 28(1): 140-152. (In Persian)
Arnon, D.I. 1949. copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiol. 24:1-15.
Ashrafuzzaman, M., F.A. Hossen, M.R. Ismail, M.A. Hoque, M.Z. Islam, S.M. Shahidullah, and S. Meon. 2009. Efficiency of plant growth-promoting rhizobacteria (PGPR) for the enhancement of rice growth. Afr. J. Biotechnol. 8(7): 1247-1252.
Asker, D., T. Beppu, and K. Ueda. 2007. Unique diversity of carotenoid-producing bacteria isolated from Misasa, a radioactive site in Japan. Appl. Microbiol. Biotechnol. 77: 383-392.
Bharathi, R., R. Vivekananthan, S. Harish, A. Ramanathan, and R. Samiyappan. 2004. Rhizobacteria-based bio-formulations for the management of fruit rot infection in chillies. Crop Prot. 23: 835-843.
Burd, G.I., D.G. Dixon, and B.R. Glick. 1998. A plant growth-promoting bacterium that decreases nickel toxicity in seedlings. Appl. Environ. Microbiol. 64: 3663-3668.
Chakraborty, U., B.N. Chakraborty, A.P. Chakraborty, and P.L. Dey. 2013. Water stress amelioration and plant growth promotion in wheat plants by osmotic stress tolerant bacteria. World J Microbiol Biotechnol. 29: 789-803.
Chinnaswamy, A., T.C. Pena, A. Stoll, D.P. Rojo, J. Bravo, A. Rincon, M.M Lucas, and J.J. Pueyo. 2018. A nodule endophytic Bacillus megaterium strain isolated from Medicago polymorpha enhances growth, promotes nodulation by Ensifer medicae and alleviates salt stress in alfalfa plants. Ann. Appl. Biol. 172(3): 295-308.
Choudhary, S.K., S.K. Gupta, M.K. Singh,and S. Sheraz Mahdi.2016. Role and its utilization of beneficial micro-organisms for sustainable crop production. International J Plant Growth Regul. 12(2): 370-378.
Egamberdiyeva, D. 2007. The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils. Appl. Soil. Ecol. 36: 184-189.
Flores, P.P., E.V. Cantero, J.A. Hernandez, R.P. Flores, J.L. Bucio, P.G. Juarez, and L.M. Rodriguez. 2017. Bacillus methylotrophicus M4-96 isolated from maize (Zea mays) rhizoplane increases growth and auxin content in Arabidopsis thaliana via emission of volatiles. J. Protoplasma. 254: 2201-2213.
Glick, B.R. 2012. Plant growth-promoting bacteria: mechanisms and applications. Hindawi Publishing Corporation Scientifica. 1-15.
Goswami, D., J.N. Thakker, and P.C. Dhandhukia. 2016. Portraying mechanics of plant growth promoting rhizobacteria (PGPR). Cogent. Food Agric. 2: 1-19.
Grichko, V.P., and B.R. Glick. 2001. Amelioration of flooding stress by ACC deaminase-containing plant growth-promoting bacteria. Plant Physiol. Biochem. 39: 11-17.
Gulati, A., P. Vyas, P. Rahi, and R.C. Kasana. 2009. Plant Growth-Promoting and Rhizosphere-Competent Acinetobacter rhizosphaerae Strain BIHB 723 from the Cold Deserts of the Himalayas. Curr Microbiol. 58: 371-377.
Hafeez, F.Y., M.E. Safdar, A.U. Chaudhary, and K.A. Malik. 2004. Rhizobial inoculation improves seedling emergence nutrient uptake and growth of cotton. Austr. J. Exp. Agric. 44(6): 617-622.
Ibrahim, E.A. 2015. Seed priming to alleviate salinity stress in germinating seeds. J. Plant Physiol. 192: 38-46.
Indiragandhi, P., R. Anandham, M. Madhaiyan, and T.M. Sa. 2008. Characterization of Plant Growth–Promoting Traits of Bacteria Isolated from Larval Guts of Diamondback Moth Plutella xylostella (Lepidoptera: Plutellidae). Curr. Microbiol. 56: 327-333.
Kalsa, K.K., and B. Abebie. 2012. Influence of seed priming on seed germination and vigor traits of Vicia villosa ssp. dasycarpa (Ten.). Afr. J. Agric. Res. 7(21): 3202-3208.
Kang, S., A.L. Khan, M. Hamayun, Z.H. Shinwari, Y.H. Kim, G.J. Joo, and I.J. Lee. 2012. Acinetrobacter calcoaceticus ameliorated plant growth and influenced gibberellins and functional biochemical. Pak. J. Bot. 44(1): 365-372.
Kang, S.M., G.J. Joo, M. Hamayun, C.I. Na, D.H. Shin, H.Y. Kim. J.K. Hong, and I.J. Lee. 2009. Gibberellin production and phosphate solubilization by newly isolated strain of Acinetobacter calcoaceticus and its effect on plant growth. Biotechnol. Lett. 31: 277-281.
Kang, S.M., R. Radhakrishnan, Y.H. You, G.J. Joo, I.J. Lee, K.E. Lee, and J.H. Kim. 2014. Phosphate Solubilizing Bacillus megaterium mj1212 Regulates Endogenous Plant Carbohydrates and Amino Acids Contents to Promote Mustard Plant Growth. Indian J Microbiol. 54(4): 427-433.
Kim, M.J., R. Radhakrishnan, S.M. Kang, Y.H. You, E.J. Jeong, J.G. Kim, and I.J. Lee. 2017. Plant growth promoting effect of Bacillus amyloliquefaciens H-2-5 on crop plants and influence on physiological changes in soybean under soil salinity. Physiol Mol Biol Plants. 23(3): 571-580.
Kumar Jha, C., and M. Saraf. 2015. Plant growth promoting Rhizobacteria (PGPR). J. Agric. Res. Develop. 5(2): 108-119.
Kumar, K.V., SH. Srivastava, N. Singh, and H.M. Behl. 2009. Role of metal resistant plant growth promoting bacteria in ameliorating fly ash to the growth of Brassica juncea. J. Hazard. Mater. 170: 51-57.
Lichtenthaler H.K. 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods. Enzymol. 148:350-382.
Makkizadeh Tafti, M., R. Farhoudi, and M. Rastifar. 2012. Effect of osmopriming on seed germination of lemon bahm (Melissa officinalis L.) under salinity stresses. Iranian J. Med.d Aromatic Plants. 27(4): 573-586. (In Persian)
Meryem, S.SH., and A. Yasmin. 2013. Effect of lead resistant bacteria on the early growth of Vigna mungo L. (hepper) under lead stress. Pak. J. Bot. 45(S1): 481-485.
Mustafa, H.S.B., T. Mahmood, A.Ullah, A. Sharif, A.N. Bhatti, M. Nadeem, and R. Ali. 2017. Role of seed priming to enhance growth and development of crop plants against biotic and abiotic stresses. Bull. Biol. Allied. Sci. Res. 2: 1-11.
Nehra, V., B.S. Saharan, and M. Choudhary. 2016. Evaluation of Brevibacillus brevis as a potential plant growth promoting rhizobacteria for cotton (Gossypium hirsutum) Crop. SpringerPlus. 5: 2584-2588.
Panpan, Z., Y. Bo, X. Mengke, C. Xiaoying, X. Lingwei, and J. Jihong. 2017. Evaluation of the effect of plant growth promoting endophytic bacteria from pinellia ternate using an efficient organic silica hybrid monolithic column. J. Biobased. Mater. Bio. 11(4): 282-290.
Park, Y.G., B.G. Mun, S.M. Kang, A. Hussain, R. Shahzad, C.W. Seo, A.Y. Kim, S.U. Lee, K.Y. Oh, D.Y. Lee, I.J. Lee, and B.W. Yun. 2017. Bacillus aryabhattai SRB02 tolerates oxidative and nitrosative stress and promotes the growth of soybean by modulating the production of phytohormones. J. Pone. 1-28.
Pii, Y., T. Mimmo, N. Tomasi, R. Terzano, S. Cesco, C. Crecchio. 2015. Microbial interactions in the rhizosphere: beneficial influences of plant growth-promoting rhizobacteria on nutrient acquisition process. Biol Fertil Soils. 51: 403-415.
Rahimi, M., and A. Athari. 2008. Medical microbiology. Ayizh Publishing. pp 1050. (In Persian)
Rahimian Mashhadi, H., A. Bagheri Kazemabad, and A. Paryab. 1991. Effects of PEG and NaCl induced water potential at different temperatures on germination and seedling vigor of several wheat (Triticum spp.) populations. J. Agric. Sci. Technol. 5(1): 37-47.
Sadiq, H.M., G.Z. Jahangir, I.A. Nasir, M. Iqtidar, and M. Iqbal. 2013. Isolation and characterization of phosphate solubilizing bacteria from rhizosphere soil. Biotechnol. Biotec. Eq. 27(6): 4248-4255.
Saleem, M., H.N. Asghar, Z.A. Zahir, and M. Shahid. 2018. Impact of lead tolerant plant growth promoting rhizobacteria on growth, physiology, antioxidant activities, yield and lead content in sunflower in lead contaminated soil. J. Chemosphere. 195: 606-614.
Sarkar, A., P.K. Ghosh, K. Pramanik, S. Mitra, T. Soren, S. Pandey, M.H. Mondel, and T.K. Maiti. 2018. A halotolerant Enterobacter sp. displaying ACC deaminase activity promotes rice seedling growth under salt stress. Res. Microbiol. 169: 20-32.
Sarsan, S. 2016. Effect of phosphate solubilizing bacteria Bacillus PSB24 on growth of tomato plants. Int.J.Curr.Microbiol.App.Sci. 5(7): 311-320.
Shah, S., J. Li, B.A. Moffatt, and B.R. Glick. 1998. Isolation and characterization of ACC deaminase genes from two different plant growth-promoting rhizobacteria. Can. J. Microbiol. 44(9): 833-843.
Shahzad, R., M. Waqas, A.L. Khan, S. Asaf, M.A. Khan, S. Kang, B. Yun, and I. Lee. 2016. Seed-borne endophytic Bacillus amyloliquefaciens RWL-1 produces gibberellins and regulates endogenous phytohormones of Oryza sativa. Plant Physiol. Biochem. 106: 236-243.
Sivakumar, T., S. Ambika, and K. Balakrishnan. 2017. Biopriming of rice seed with Phosphobacteria for enhanced germination and vigour. Springerplus. 54(3): 346-349.
Sivasakthi, S., G. Usharani, and P. Saranraj. 2013. Biocontrol potentiality of plant growth promoting bacteria (PGPR) – Pseudomonas fluorescens and Bacillus subtilis. Afr. J. Agric. Res. 9(16): 1265-1277.
Stajkovic, O., D. Delic, D. Josic, D. Kuzmanovic, N. Rasulic, and J.K. Vukcevic. 2011. Improvement of common bean growth by co-inoculation with Rhizobium and plant growth-promoting bacteria. Rom. Biotech. Lett. 16(1): 5919-5926.
Sun, Z., K. Liu, J. Zhang, Y. Zhang, K. Xu, D. Yu, J. Wang, L. Hu, L. Chen, and C. Li. 2017. IAA producing Bacillus altitudinis alleviates iron stress in Triticum aestivum L. seedling by both bioleaching of iron and up-regulation of genes encoding ferritins. Plant Soil. 419: 1-11.
Tamilarasi, S., K. Nanthakumar, K. Karthikeyan, and P. Lakshmanaperumalsamy. 2008. Diversity of root associated microorganisms of selected medicinal plants and influence of rhizomicroorganisms on the antimicrobial property of Coriandrum sativum. J. Environ. Biol. 29(1): 127-134.
Tsavklova, E., T.A. Cherdyntseva, S.Y. Klimova, A.I. Shestakov, S.G. Botina, and A.I. Netrusov. 2007. Orchid-associated bacteria produce indole-3-acetic acid, promote seed germination, and increase their microbial yield in response to exogenous auxin. Arch. Microbiol. 188: 655-664.
Van Loon, L.C. 2007. Plant responses to plant growth-promoting rhizobacteria. Eur. J. Plant Pathol. 119: 243-254.
Vejan, P., R. Abdullah, T. Khadiran, S. Ismail, and A.N. Boyce. 2016. Role of Plant Growth Promoting Rhizobacteria in Agricultural Sustainability. J. Molecules. 1-17.
Vessey, J.K. 2003. Plant growth promoting rhizobacteria as biofertilizers. Plant Soil. 255:571-586.
Wani, P.A., and M.S. Khan. 2010. Bacillus species enhance growth parameters of chickpea (Cicer arietinum L.) in chromium stressed soils. J. Food Chem. Toxicol. 48(11): 3262-3267.
Wu, S.C., Z.H. Cao, Z.G. Li, K.C. Cheung, and M.H. Wong. 2005. Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. J. Geoderma. 125: 155-166.
Zamin, F.R., D. Sachdev, N. KazemiPour, A. Engineer, K.R. Pardesi, S. Zinjarde, P.K. Dhakephalkar, and B.A. Chopade. 2011. Characterization of Plant-Growth-Promoting Traits of Acinetobacter Species Isolated from Rhizosphere of Pennisetum glaucum. J. Microbiol. Biotechnol. 21(6): 556-566.
Zhang, J., P. Wang, L. Fang, Q. Zhang, C. Yan, and J. Chen. 2017. Isolation and characterization of phosphate solubilizing bacteria from mushroom residues and their effect on tomato plant growth promotion. Pol. J. Microbiol. 66(1): 57-65.
Zhao, L., Y. Xu, and X. Lai. 2018. Antagonistic endophytic bacteria associated with nodules of soybean (Glycine max L.) and plant growth-promoting properties. Braz. J. Microbiol. 49: 269-278.
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