Iranian Journal of Seed Science and Technology

Current Issue

By Issue

By Author

By Subject

Author Index

Keyword Index

About Journal

Aims and Scope

Editorial Board

Publication Ethics

Indexing and Abstracting

Related Links

FAQ

Peer Review Process

Journal Metrics

News

Terms and Condition

Instruction

Guide to APA Style

Forms

Assessment of genetic purity of Iranian commercial maize hybrid seeds using DUS characteristics and microsatellite markers

Document Type : Original Article

Authors

1 faculty member

2 Faculty member

Abstract

Genetic purity is one of the most important qualitative criteria for success in hybrid seeds production. In order to avoid reduction in yield caused by using presence of seeds resulting from self-pollinating and unwanted crossing or seed mixing, development of a simple, rapid, and accurate method for genetic purity assessment is of great importance. Accordingly, the genetic purity of 6 important Iranian commercial maize hybrid cultivars (KSC703, KSC704, KSC705, KSC706, Karun and Mobin) was evaluated by GOT and molecular markers. The results of GOT showed that the lowest and highest genetic purity belonged to Mobin (67.9%) and KSC704 (92.9%) hybrids, respectively. Out of the 11 SSR markers, 6 primers were selected since they have produced clear, scorable and unambiguous polymorphic bands among the parents, so that they could identify all hybrids from self-pollinated seeds and off-types. Due to the high correlation between GOT and molecular marker results, Hence, it is proposed that these SSR markers can be used in efficient analysis of hybrid seed purity since this technique is simple to use, more accurate and not affected by environment when compared with GOT.

Keywords

References
Assareh, M.H. 2016. Analytical Report of Seed and Plant Certification and Registration Institute (SPCRI). Publication of Seed and Plant Certification and Registration Institute.
Blair, M.W., V. Hedetale, and S.R. McCouch. 2002. Fluorescent-labeled microsatellite plants useful for detecting allelic diversity in cultivated rice (Oryza sativa L.). Theor. Appl. Genet. 105(2-3): 449-457.
Bonow, S., E.V.R. Von Pinho, M.G.C. Vieira, and B. Vosman. 2009. Microsatellite markers in and around rice genes: Applications in variety identification and DUS testing. Crop Sci. 49: 880-886.
Choukan, R. 2012. Maize seed production. Publication of Agricultural Research and Education Organization of Tehran.
Daniel, I., J. Adetumbi, O. Oyelakin, S. Olakojo, M. Ajala, and S. Onagbesan. 2012. Application of SSR Markers for Genetic Purity Analysis of Parental Inbred Lines and Some Commercial Hybrid Maize (Zea mays L.). Am. J. Exp. Agric. 2: 597-606.
Dou, X.-Y., M. Yan, Y.-F. Xu, K. Hussain, Y.-Z. Liu, and F. Lin. 2012. Identification and purity testing of maize hybrids with one parent in common by ultrathin-layer isoelectric focusing of seed salt-soluble proteins. Turk J. Agric. For. 36: 267-273.
Food and Agricultore Organization of United Nations.2018. FAOSTAT. [Online] Awailable at http://www.fao.org/faostat.
Gunjaca J., I. Buhinicek, M. Jukic, H. Sarcevic, A. Vragolovic, Z. Kozic, A.Jambrovic, and I. Pejic. 2008. Discriminating maize inbred lines using molecular and DUS data. Euphytica. 161(1-2): 165-172.
Hipi, A., M. Surahman, and S. Ilyas. 2013. Seed Genetic Purity Assessment of Maize Hybrid Using Microsatellite Markers (SSR). Int. J. Appl. 3(5): 66-71.
Komori, T., and N. Nitta. 2004. A simple method to control the seed purity of japonica hybrid rice varieties using PCR-based markers. Plant Breed. 123: 549- 553.
Kwon, Y.S., J.M. Lee, G.B. Yi, S.I. Yi, K.M. Kim, E.H. Soh, K.M. Bae, E.K. Park, I.H. Song, and B.D. Kim. 2005. Use of SSR markers to complement tests of distinctiveness, uniformity, and stability (DUS) of pepper (Capsicum annuum L.) varieties. Mol. Cell. 19: 428-435.
Liu, L.W., X.L. Hou, Y.Q. Gong, Y.M. Zhang, K.R. Wang, and J.F. Zheng. 2004. Application of molecular marker in variety identification and purity testing in vegetable crops. Mol. Plant Breed. 2: 563–568.
Mao, C.X., S.S. Virmani, and I. Kumar. 1998. Technological innovations to lower the cost of hybrid rice seed production. Advances in hybrid rice technology. Int. Rice Res. Inst. (IRRI), Manila.
McDonald, M.B. 1998. Seed quality assessment. Seed Sci. Res. 8: 265-276.
Moorthy, K.K., P. Babu, M. Sreedhar, V.S.A.K. Sama, P.N. Kumar, S.M. Balachandran, and R.M. Sundaram. 2011. Identification of informative EST-SSR markers capable of distinguishing popular Indian rice varieties and their utilization in seed genetic purity assessments. Seed Sci. Technol. 39: 282–292.
Mulsanti, I.W. 2011. Identification and evaluation of genetic purity of hybrid rice seeds using microsatellite markers (Doctoral dissertation, Thesis).
Pendse, R., S. Malhotra, S. Pawar, and T. Krishna. 2001. Use of DNA markers for identifying inbreds and hybrid seeds in cotton (Gossypium hirsutum L.). Seed Sci. Technol. 29: 503–508
Rana, M., S. Singh, and K. Bhat. 2007. RAPD, STMS and ISSR markers for genetic diversity and hybrid seed purity testing in cotton. Seed Sci. Technol. 35: 709-721.
Rotondi, A., M. Magli, C. Ricciolini, and L. Baldoni. 2003. Morphological and molecular analyses for the characterization of a group of Italian olive cultivars. Euphytica. 132: 129-137.
Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgensen, and R.W.L. Allard. 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. 81(24): 8014-8018.
Salgado, K.C.P.D.C., D.G.G.C. Vieira, É.V.D.R. Von Pinho, C.T. Guimarães, R.G. Von Pinho, and L.V.D. Souza. 2006. Genetic purity certificate in seeds of hybrid maize using molecular markers. Rev. Brasileira de Sementes, 28(1): 169-175.
Silvanacristae, S.M., J.F.M. Moistsai, M.A. Valls, A. Marcos, and C.R. Lopes. 2005. Genetic characterization of Brazilian annual Arachis species from selections Arachis and heteranthae using RAPD markers. Genet. Resour. Crop. Ev. 52(8): 1079-1086.
Singh, R.K., R.K. Sharma, A.K. Singh, V.P. Singh, N.K. Singh, S.P. Tiwari, and T. Mohapatra. 2004. Suitability of mapped sequence tagged microsatellite site markers for establishing distinctness, uniformity and stability in aromatic rice. Euphytica. 135: 135-143.
Sundaram, R., B. Naveenkumar, S. Biradar, S. Balachandran, B. Mishra, M. IlyasAhmed, B. Viraktamath, M. Ramesha, and N. Sarma. 2008. Identification of informative SSR markers capable of distinguishing hybrid rice parental lines and their utilization in seed purity assessment. Euphytica. 163: 1–10
Taixing, Y., G. Lequn, Z. Zhong, and G. Mingguang. 1998. Comparison between varietal purity and zymogram purity in maize. Chinese Sci. Bull. 43: 930-935.
Tanksley S.D, and S.R. McCouch. 1997.  Seed banks and molecular maps: Unlocking Genet. potential from the wild. Sci. 277: 1063 –1066
Tommasini, L., J. Batley, G.M. Arnold, R.J. Cooke, P. Donini, D. Lee, J.R. Law, C. Lowe, C. Moule, M. Trick, and K.J. Edwards. 2003. The development of multiplex simple sequence repeat (SSR) markers to complement distinctness, uniformity and stability testing of rape (Brassica napus L.) varieties. Theor. Appl. Genet. 106: 1091-1101.
UPOV (International Union for the Protection of new Varieties of Plants). 2009. Guidelines for the conduct of tests for Distinctness, Uniformity and Stability In maize. TG/2/7. Geneva, Switzerland.
UPOV-BMT, 2002. BMT/36/10 Progress report of the 36th session of the technical committee, the technical working parties and working group on biochemical and molecular techniques and DNA-profiling in particular, Geneva.
Wu, M., X. Jia, L. Tian, and B. Lv. 2010. Rapid and reliable purity identification of F1 hybrids of Maize (Zea may L.) using SSR markers. Maize Genomics and Genet. 1: 1-4
Zadoks, J.C., T.T. Chang, and C.F. Konzak. 1974. A decimal code for the growth stages of cereals. Weed Res.14 (6): 415-421.
Iranian Journal of Seed Science and Technology
Volume 8, Issue 2 - Serial Number 16
December 2019
Pages 229-240
Files
Share
How to cite
Statistics