The effect Avena sativa L. seeds coating with amylose and agar on seeds germination and physiologic indexes in alkaline stress

Document Type : Original Article

Authors

1 biology department, Saveh branch, Islamic Azad university, Saveh,Iran

2 biology department, Saveh branch, Islamic azad university, Saveh, Iran

3 biology department, Saveh branch, Islamic Azad university, Saveh, Iran

Abstract

The aim of this study was the evolution of effects of seed coating with starch (amylose) and agar on germination and physiological indexes in alkaline stress. This factorial study was conducted in a randomized block design. Factors included seed coating at three levels (control, agar and starch coat) and alkaline stress (the mediums with pH values of 6-10 with sodium carbonate and sodium bicarbonate with values of 0-90 mMol). By sowing seeds for 7 days in petri dishes and 20 days in pots, seed potency, amylase activity, germination rate, growth, minerals and chlorophyll a and b were evaluated. Starch and agar reduced the effect of alkali stress. They increased seed potency (84%), amylase activity (53 mg / min), germination rate (11.32 seeds per day) and growth (23 cm) in medium with pH = 10 with a significant difference.also they prevented the decomposition of chlorophyll and reduces the absorption of minerals. Starch coating was more suitable for sowing seeds under normal conditions and alkaline stress, and agar coating was more suitable for maintaining seed vigor and seedling growth in higher amounts of alkaline.

Keywords


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