Effect of different priming methods and temperatures on seed dormancy breakage and germination of Pulicaria gnaphalodes (Vent.) Boiss

Document Type : Original Article

Authors

1 PhD student of medicinal plants, Department of Horticulture, Islamic Azad University Birjand Branch, Birjand, Iran.

2 Associated Professor, Department of Agriculture, Birjand Branch, Islamic Azad University, Birjand, Iran.

3 Assistant Professor of Horticulture, Islamic Azad University, Birjand Branch, Birjand, Iran.

Abstract

Seed germination is the first and most sensitive developmental stage in the plant life cycle and the key process in seedling emergence. On the other hand, one of the major barriers to optimum use of medicinal plants outside the natural habitat is limited level of seed germination and seed dormancy. Hence in order to find the best priming and temperature treatment to dormancy breaking and seed germination of medicinal plant seed Pulicaria gnaphalodes (Vent.) Boiss a factorial experiment based on the completely randomized design with four replications and two factors was conducted in Islamic Azad University, Birjand branch in 2017. The first factor of priming type is 11 levels included in potassium nitrate 2% treatment at two times of 24 and 48 hours, gibberellic acid (0, 250 and 500 mg/ l) at two times of 24 and 48 hours, and stratification (7, 14 and 21 days). The second factor was temperature at three levels (15, 20 and 25 °c). The results showed that highest germination rate and percent germination were related to stratification at 7 and 21 days at 25 °c and maximum seedling length, plumule length, radicle length, radicle/plumule length ratio, wet weight of seedling, dry weight of seedling, percent germination, germination rate, longitudinal index seed vigor, weight index seed vigor related to 21 days wet temperature and 15°c. Therefore, 21 days stratification and temperatures of 15 and 25 °c were the best priming and temperature treatments to dormancy breaking and seed germination of medicinal plant seed Pulicaria gnaphalodes.

Keywords

References

Adebisi, M.A., F.S. Okelola, M.O. Ajala, T.O. Kehinde, I.O. Daniel, and O.O. Ajani. 2013. Evaluation of variations in seed vigour characters of West African rice(Oryza sativa L.)genotypes using multivariate technique. J. Plant Sci. 4: 356-363.
Aghilian, S., M. Khajeh-Hosseini, and S. Anvarkhah. 2014. Evaluation of seed dormancy in forty medicinal plant species. J. Agric. Crop Sci. 7(10): 760-768.
Asaadi, A.M., and Gh.A. Heshmati. 2015. The effect of different treatments on breaking seeds dormancy and inducing germination of Thymus transcaucasicus Ronn. and Zataria moltiflora Boiss. J. Plant Res. (Iranian J. Biol.). 28 (1): 12-22. (In Persian, with English Abstract)
Asadi Aghbolaghi, M., and M. Sedghi. 2014. The effect of osmo and hormone priming on germination and seed reserve utilization of millet seeds under drought stress. J. Stress Physiol. Biochem. 10(1): 214-221.
Asghari, G., F. Zahabi, A. Eskandarian, H. Yousefi, and M. Asghari. 2014. Chemical composition and leishmanicidal activity of Pulicaria gnaphalodes essential oil. Res. J. Pharmacognosy. 1(4): 27-33.
Baghari, Z., A. Mahmoodzade, and M. Nojavan. 2000. The effect of different physical and chemical treatments on seed breakage and seed germination of species Amaranthus retroflexus, Melilotus Officinalis, Datura Stramonium. M.Sc.Dissertation, Univ. of Urmia, Iran. (In Persian, with English Abstract)
Batooli, H., A. Haghir Ebrahim Abadi, E. Karimi Khozani, and A. Mazoochi. 2017. The Survey of the essential oil composition of Pulicaria gnaphalodes (Vent.) Boiss from Brzok of Kashan. Eco-Phytochemi. J. Med. Plants. 5(17): 65-77. (In Persian, with English Abstract)
Bewley, J. D., and M. Black. 1994. Seeds: Physiology of development and germination. Elsevier Scientific Publication B.V., Amsterdam, Netherlands.
Di Girolamo, G., and L. Barbanti. 2012. Treatment conditions and biochemical processes influencing seed priming effectiveness. Ital. J. Agron. 7(25): 178-188.
Emek, Y. 2017. Effect of temperature on in vitro seed germination and mean germination time of endemic medicinal plant Nepeta viscida Boiss. (Lamiaceae). J. Biotechnol. Biosci. 5(5): 49-52.
Farhoudi, R., and M. Makizadeh Tafti. 2014. EvalutionKelussia odoratissma seed dormancy breaking under gibberellin acid and stratification. J. Seed Sci. Technol. 3(2): 241-249. (In Persian, with English Abstract)
Farhoudi, R., A. Modhej, and A. R. Jamshidi. 2015. Echinacea purpurea seed pretreatment to improve germination. J. Fish. Hydrobiol. 10(9): 58-61.
Ghahremani, A., E. Ganji Moghadam, M. Tatari, and S. Khosroyar. 2018. Effect of chilling and chemical treatment on seed dormancy of Withania somnifera. J. Seed Res. 8(1): 47-60. (In Persian, with English Abstract)
Greipsson, S. 2001. Effects of stratification and GA3 on seed germination of a sand stabilising grass Leymus arenarius used in reclamation.J. Seed Sci. Technol. 29: 1-10.
Hadi, N., M. Souri, and R. Omidbeigi. 2011. The effect of cold stratification and gibberellic acid pretreatments on seed germination of Archangelica, Tanacetum cinerariaefolium and Chelidonium majus. J. Hort. Sci. (Agric. Sci. Technol.). 25(4): 397-403. (In Persian, with English Abstract)
ISTA .1996. International rules for seed testing. Seed Sci. Technol. 13: 299-513.
Jabbari, R, M. Amini Dehaghi, F. Ganji Arjenaki1, and K. Agahi. 2011. How duration and methods of priming may affect the germination of cumin seeds (Cuminum cyminum L.). J. Agron. Sci. 4(4): 23-30. (In Persian, with English Abstract)
Karimian Farimam, Z., M. Azizi, and S. Noori. 2011. Seed germination and dormancy breaking techniques for Echinacea purpurea L. J. Biol. Environ. Sci. 5(13): 7-10.
Khaninejad, S., I.H. Arefi, and M. Kafi. 2012. Effect of priming on dormancy breaking and seedling establishment of caper (Capparis spinosa L.). Int. Conf. Appl. Life Sci., Turkey, 10-12 September: 365-370.
Khanna, P.K., A. Kumar, R. Chandra, and V. Verma. 2013. Germination behavior of seeds of Withania somnifera (L.) Dunal: a high value medicinal plant. Physiol. Mol. Biol. Plants. 19(3): 449–454.
Khatibzadeh, R., Azizi, M., and H. Arouei. 2013. The effect of surface disinfection treatments and cold stratification on seed germination (Levisticum officinale Koch.) invitro conditions. J. Hort. Sci. 27(2): 130-138. (In Persian, with English Abstract)
Khorramdel, S., A. Nezami, and A. Mollafilabi. 2013. Evaluation of germination characteristics for some Khorasan's Cumin (Cuminum cyminum L.) seed landraces under fall planting dates. Res. Crop Ecosyst. 1(1): 55-67. (In Persian, with English Abstract)
Makkizadeh, M., R. Farhoudi, H.A. Naghdi badi, and A. Mehdizadeh. 2006. Assigning the best treatment for increasing germination of three medicinal plants seeds: Rubia tinctourum L., Echinacea angustifolia D.C. and Myrtus communis L. J. Med. Aromatic Plants. 22(2): 105-116. (In Persian, with English Abstract)
Mellati Nokhandan, H., M. Kafi, and F. Najafi. 2013. Study of dormancy breaking methods and measurement of the germination index (Nepeta bracteata Benth.).M.Sc. Dissertation, Ferdowsi Univ. of Mashhad, Iran. (In Persian, with English Abstract)
Moravcova, L., P. Pysek, L. Krinke, J. Pergl, I. Perglova, and K. Thompson. 2007. Seed germination, dispersal and seed bank in Heracleum mantegazzianum. Pp 74-91. In P. Pysek, M.J.W. Cock, W. Nentwig, and H.P. Ravn (ed.) Ecology and management of giant hogweed (Heracleum mantegazzianum). CABI, Wallingford.
Mumivand, H., A.R. Rustaii, K. Jahanbin, and D. Dastan. 2010. Essential oil composition of Pulicaria dysenterica L. from Iran. J. Essential Oil Bearing Plants. 13(6): 717–720.
Nabaei, M., P. Roshandel, and A.R. Mohammadkhani. 2013. The effects of plant growth regulators on breaking seed dormancy in Silybum marianum L. J. Cell Tissue. 4(1): 45-54. (In Persian, with English Abstract)
Nabaei, M., P. Roshandel, and A.R. Mohammadkhani. 2014. Effect of chemical treatments, pre-moist chilling, hot and tap water on seed dormancy breaking inArctium lappa. J. Plant Res. (Iran. J. Biol.). 26(2): 217-225. (In Persian, with English Abstract)
Nadjafi, F., M. Bannayan, L.Tabrizi, and M. Rastgoo. 2006. Seed germination and dormancy breaking techniques for Ferula gummosa and Teucrium polium. J. Arid Environ. 64: 542–547.
Nasiri, M., H. Madah Aarefi, and H.R. Eisavand. 2004. Investigation of changes in seed potency and seed dormancy of some species in the natural resources gene bank. J. Range. For. Plants Breed. Genet. Res. 12(2): 163-182. (In Persian, with English Abstract)
Nicolas, C., G. Nicolas, and D. Rodriguez. 1996. Antagonistic effects on abscisic acid and gibberellic acid on the breaking of dormancy of Fagus sylvatica seeds. Physiol. Plant. 96: 244-250.
Rajabian, T., A. Saboora, B. Hassani, and H. Fallah Hosseini. 2007. Effects of GA3 and chilling on seed germination of Ferula assa-foetida, as a medicinal plant. J. Med. Aromatic Plants. 23(3): 39-404. (In Persian, with English Abstract)
Ravandeh, M., J. Valizadeh, M. Noroozifar, and M. Khorasani Motlagh. 2011. Screening of chemical composition of essential oil, mineral elements and antioxidant activity in Pulicaria Undulata L. from Iran. J. Med. Plant Res. 5(10): 2035-2040.
Razavi, S.M., and R. Hajiboland. 2009. Dormancy breaking and germination of Prangos ferulaceae seeds. J. Biosci. 3: 78-83.
Sajjadi Jaghargh, S.S., M.A. Alizadeh, and M. Kalagari. 2013. Assessment of seed emergence characteristics and seedlings vigor of three populations aromatic medicinal plant species of Anthemis pseudocotula Boiss by using of priming technique and pre-chilling. Bull. Environ. Pharmacol. Life Sci. 3(1): 143-150.
Salar Bashi, D., A. Ghani, and J. Asili. 2013. Essential oil composition of Pulicaria gnaphalodes (Vent.) Boiss. growing in Iran. J. Essential Oil-Bearing Plants. 16(2): 252–256.
Sharifi, M., and M. Pouresmael. 2003. Effects of some chemical substances on dormancy breaking
and induction of seed germination in Bunium persicum (Boiss.) fedtsch. J. Agric. Sci. Natur. Resour. 10(2): 33-41.
Sudharani, M., and A. Padmasri. 2014. Assessment of seed vigor tests for relative storability and field performance in cotton. J. Agric. Veter. Sci. 7 (8): 1-4.
Tipirdamaz, R., and A.N. Gomurgen. 2000. The effects of temperature and gibberellic acid on germination of Eranthis hyemalis seeds. Res. Note Turk Bot. 24: 143–145.
Toole, E.H., V.K. Toole, H.A. Borthwick, and S.B. Hendricks. 1995. Photocontrol of Lepidium sp. seed germination. Plant Physiol. 30(1): 15-21.
Windauer, L., A. Altuna, and R. Benech-Arnold. 2007. Hydrotime analysis of Lesquerella fendleri seed germination responses to priming treatments. Indust. Crop Product. 25(1): 70–74.
Yamauchi, Y., M. Ogawa, A. Kuwahara, A. Hanada, Y. Kamiya, and Sh. Yamaguchi. 2004. Activation of gibberellin biosynthesis and response pathways by low temperature during imbibition of Arabidopsis thaliana seeds. The Plant Cell. 16: 367–378.
Iranian Journal of Seed Science and Technology
Volume 10, Issue 1 - Serial Number 21
Spring of 2021
April 2021
Pages 59-70

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