Effect of biological pre-treatment of seed on germination and growth indices and pigments of flaxseed (Linum usitatissimum L.) under salinity stress

Document Type : Original Article

Authors

1 shahed university

2 Associate Professor ,Agricultural College and Medicinal Plant Research Center, Shahed University, Tehran

Abstract

To study the effect of biological treatment on germination and growth indices and pigments of flaxseed under salinity stress, a factorial experiment in a completely randomized design was conducted in three replications at the faculty of agriculture of Shahed University in 2018. Experimental treatments were salinity at four levels (zero, 2.5, 5 and 7.5 dS/m) and biological treatments at four levels (control, bacteria (Baccillus subtilis), fungi (Macrophomina phaseolina) and combination of bacteria and fungi). Effect of biological treatment was significant on germination percentage and the highest germination percentage (99 %) obtained at fungi treatment. Effect of salinity and biological treatment was significant on mean germination time and germination coefficient. The minimum mean germination time (3.79 days) obtained at combination of bacteria and fungi treatment that shows this treatment was most effective in compared with other biological treatments. With increasing of salinity, mean germination time increased. Salinity reduced radicle, plumule and seedling length. The means related to radicle length at the different levels of salinity at biological treatment of fungi was highest amount in compared with other biological treatments. Interaction effect of salinity and biological treatment was significant on chlorophyll a and total chlorophyll. With increasing of salinity, reduced pigments. Maximum amount of chlorophyll a (16.52 µm.ml1-) was obtained at the control treatment of biological treatment and zero salinity. The result of this research showed that using of microorganisms such as fungi and bacteria in salinity stress, can reduce the negative effects of salinity on germination and growth of this plant.

Keywords

References

Arnon, A. N. 1967. Method of extraction of chlorophyll in the plants. Agron. J. 23:112-121.
Ashraf, M., and M. R. Foolad. 2005. Pre-sowing seed treatment- A shotgun approach to improve germination growth and crop yield under saline and none-saline conditions. Adv. Agron. 88: 223-271.
Aghighi Shahverdi, M., M. S. Heydari, and A. Tobeh. 2011. The impact of the Azotobacter on germination indices of lentils. Proc. Conf. Sustainable Manage. Nat. Resour. Gorgan Univ. Agric Nat. Resour. Sci, Gorgan, Iran. (In Persian)
Ansari, O., H. R. Choghazardi, F. Sharifzadeh, and H. Nazarli. 2012. Seed reserve utilization and seedling growth of treated seeds of mountain ray (Seeca lemontamum) as affected by drought stress. Cercet. Agron. Mold. 2(150): 43-48.
Assadian, N. W, and S. Miamato. 1983. Salt effect on alfalfa seedling emergence. Agron. J. 79: 710-714.
Azarnivand, H, and M. Ghorbani. 2007. Effect of sodium on the germination of both species. Iranian J. Range. Desert. 4(3): 352-358. (In Persian)
Bacilio, M., M. Rodriguez., M. Morero., J. P. Hernandez, and Y. Bushan. 2001. Mitigation of salt stress in wheat seedling by agfp-taged Azosprillium lipoferum. Biol. Fert. Soil. 40:188-193.
Barassi, C. A., G. Ayrault., C. M. Creus., R. J. Sueldo, and M. T. Sobrero. 2006. Seed inoculation with Azospirillium mitigates NaCl effects on lettuce. Sci. Hortic. 109: 8-14.
Boomsma, C. R, and T. J. Vyn. 2008. Maize drought tolerance: Potential improvements through Arbuscular mycorrhizal symbiosis. Field Crops Res. 108: 14-31.
Bybordi, A, and J. Tabatabaei. 2009. Effect of salinity stress on germination and seedling properties in canola (Brassica napus L.). Not. Bot. Hortic. Agrobot. Cluj-Napoca. 37(2): 71-76.
Creus, C.M., M. Graziano., E.M. Casanovas., M.A. Pereyra., M. Simontacchi., S. Puntarulo., C.A. Barassi, and L. Lamattina. 2005. Nitric oxide is involved in the Azospirillum brasilense induced lateral root formation in tomato. Planta. 221(2): 297-303.
Cheng, Z., E. Park, and B.R. Glick. 2007. 1-Aminocyclopropane-1- carboxylate deaminase from Pseudomonas putida UW4 facilitates the growth of canola in the presence of salt. Can. J. Microbiol. 53: 7. 912-918.
Ehteshami, S. M. R., P. Tusi., Z. Amin Deldar, and K. Khavazi. 2010. The effect of seed inoculation with PGPR on germination and seedling growth of rapeseed (Brassica napus L.) at different levels of salinity. The first Conf. Oilseeds, Isfahan, Center of Excellence for oil seeds, Isfahan University. (In Persian)
Ellis, R. H, and E. H. Roberts. 1981. The quantification of ageing and survival in orthodox seeds. Seed Sci. Technol. 9: 377-409.
Fallahi, J., M. T. Ebadi, and R. Ghorbani. 2009. The effect of salinity and drought stresses on germination and seedling growth of clary. Environ. Stress Crop Sci. 1(1): 57-67. (In Persian, with English Abstract)
Feizi, E, and A. Moradi. 2017. Effect of priming on germination and some biochemical traits of Trigonella gracum seeds. The Second International Congress and 14th National Congress on Agriculture and Plant Breeding in Iran. 9-11 September. University of Guilan. Rasht. Iran. (In Persian)
Galeshi, S. 2015. Effect of environmental stresses on plants. Gorgan University of Agriculture Science and Natural Resource, Gorgan, Iran. (In Persian)
Grover, M., S. Z. Ali., V. Sandhya., A. Rasul, and B. Yenkateswarlu. 2010. Role of microorganisms in adaptation of agriculture crops to abiotic stress. World J. Microbiol. Biotechnol. 27: 1231-1240.
Habibi, H., H. Ataei. 2017. The effect of surfactant on flax oil and chicory seed germination indices under salinity stress. Iranian J. Seed Sci. Res. 3(4): 1-13. (In Persian)
Hilhorst, H. W. M, and P. E. Toorop. 1997. Review on dormancy, germinability and germination in crop and weed seeds. Adv. Agron. 61: 111-165.
Hussein, A. Z., I. Khan., M. Ashraf., M. H. Rashid, and M. S. Akhtar. 2004. Effect of salt stress on some growth attributes of sugarcane cultivars CP-77-400 and Coj-84. Int. J. Agric. Biol. 6(1): 188-191.
Irannejad, H., M. Poshtkuh., P. Piri, and Z. Javanmardi. 2007. Farm herbal cannabis oil, Flax oil and Castor. Tehran University Press. (In Persian)
ISTA, 2010. International rules for seed testing. Supp. Seed Sci. Technol. 21: 1-288.
Kuan, K.B., R. Othman., K. Abdul Rahim, and Z.H. Shamsuddin. 2016. Plant Growth-Promoting Rhizobacteria Inoculation to Enhance Vegetative Growth, Nitrogen Fixation and Nitrogen Remobilisation of Maize under Greenhouse Conditions. PLoS ONE, 11(3): 1-19.
Kaya, M. D., S. Day., Y. Cikili, and N. Arslan. 2011. Classification of some linseed (Linum usitatissimum L.) genotypes for salinity tolerance using germination, seedling growth and ion content. Chilean J. Agric. Res. 72(1):27 32.
Kholova, J., R. K. Sairam., R. C. Meena, and G. C. Srivastava. 2009. Response of maize genotypes to salinity stress in relation to osmolytes and metal ions contents, oxidative stress and antioxidant enzymes activity. Biol. Plant. 53: 249-256.
Mehboob, I., M. Naveed, and Z. Ahmad Zahir. 2009. Rhizobial Association with Non-Legumes: Mechanisms and Applications. Criti. Rev. Plant Sci. 28:432–456.
Makar, T. K., O. Tura, and Y. Ekmekcd. 2009. Effects of water deficit induced by PEG and NaCl on chickpea (Cicer arietinum L.) cultivars and lines at early seedling stages. Gazi University J. Sci. 22(1): 5-14.
Mastouri, F., T. Bjorkman, and G. E. Harman. 2010. Seed treatment with Trichoderma harzianum alleviates biotic, abiotic and physiological stresses in germination seeds and seedlings. Biol. Control. 100(11): 1213-1221.
Mayak, S., T. T. Bernard, and R. Glick. 2004. Plant growth promoting bacteria confer resistance in tomato plants to salt stress. Plant Physiol. Biochem. 42, 565-572.
Nadeem, S. M., Z. A. Zahir., M. Naveed., M. Arshad, and S. M. Shahzad. 2006. Variation in growth and ion uptake of maize due to inoculation with plant growth promoting rhizobacteria under salt stress. Soil. Environ. 25(3): 78-84.
Mokhtari, I., P. Abrishamchi, and A. Ganjeali. 2008. The effect of calcium on amelioration of injuries salt stress on seed germination of tomato (Lycopersicon esculantom L.). J. Sci. Ind. Agric. 22(1): 89-100.
Moradi, A., and R. Piri. 2018. Enhancement of salinity stress tolerance in Cumin (Cuminum cyminum L.) as affected by plant growth promoting rhizobacteria during germination stage. J. Plant Proc. Func. 6(22): 47-53.
Munns, R. 2002. Comparative physiology of salt and water stress. Plant Cell. Environ. 28: 239-250.
Nasri, N., S. Maatallah., I. Saidi, and M. Lachaal. 2017. Influence of salinity on germination, seedling growth, ion content and acid phosphatase activities of Linum usitatissimum L. J. Anim. Plant Sci. 27(2): 517-521.
Nematolahi, Z., and G. H. Saidi. 2010. Evaluation of drought tolerance are some of genotypes of Flax oile (Linum usitatissimum L.). J. Water Agric. 25(1): 57-65. (In Persian)
Oksu, G., M. D. Kaya, and M. Atak. 2005. Effects of salt and drought stresses on germination and seedling growth of pea (Pisum sativum L.).Turkish J. Agric. For. 29(4): 237-242.
Omidbeigi, R. 2005. Production and processing of medicinal plant. Astane Ghods-e Razavi Publications, Mashhad. (In Persian)
Palacios, O.A., F.J. Choix., Y. Bashan, and L.E. Bashan. 2016.  Influence of tryptophan and indole-3-acetic acid on starch accumulation in the synthetic mutualistic Chlorella sorokiniana–Azospirillum brasilense system under heterotrophic conditions. Res. Microbiol. 167(5):367-379.
Patade, V. Y., K. Maya, and A. Zakwan. 2011. Seed priming mediated germination improvement and tolerance to subsequent to cold and salt stress in capsicum. Res. J. Seed Sci. 4(3): 125-136.
Qasim, M., M. Ashraf., M. Y. Ashraf., S. U. Rehman, and E. S. Rha. 2003. Salt–induced changes in two canola cultivars differing in salt tolerance. Biol. Plant. 46(4): 692-632.
Sahin, U., Ekinci, M., Kiziloglu, F.M., Yildirim, E., Turan, M., Kotan, R., Ors, S., 2015. Ameliorative Effects of Plant Growth Promoting Bacteria on Water-yield Relationships, Growth, and Nutrient Uptake of Lettuce Plants under Different Irrigation Levels. Hortic. Sci. 50(9): 1379-1386.
Sebei K., A. Debez., W. Herchi., S. Boukhchina, and H. Kallel. 2007. Germination kinetics and seed reserve mobilization in two flax (Linum usitatissimum L.) cultivars under moderate salt stress. J. Plant Biol. 50: 447–454.
Scotl, S. J., R. A. Jones, and W. A. Williams. 1984. Review of data analysis methods for seed germination. Crop Sci. 24(6):1192-1199.
Stephanie, E.B., V. P. Svoboda, A. T. Paul, and W. V. I. Marc. 2005. Controlled drought affects morphology and anatomy of Salvia solendens. Soc. Hortic. Sci. 130(5): 775-781.
Valdiani, A.R., A. Hassanzadeh, and M. Tajbakhsh. 2005. Study on the effects of salt stress in germination and embryo growth stages of the four prolific and new cultivars of winter rapeseed (Brassica napus L.). Pajouhesh and Sazandegi. 66: 23-32. (In Persian)
Verbon, E.H, and L.M. Liberman. 2016. Beneficial microbes affect endogenous mechanisms controlling root development. Trends. Plant Sci. 21(3): 218-229.
Wagar, A., B. Shahroona., Z. Z. Zahir, and M. Arshad. 2004. Inoculation with ACC deaminase containing rhizobacteria for improving growth and yield of wheat. Pakistan J. Agric. 41: 119-124.
Zahir, Z.A., U. Ghani, M. Naveed., S.M. Nadeem, and H.N. Asghar. 2009. Comparative effectiveness of Pseudomonas and Serratia sp. Containing ACCdeaminase for improving growth and yield of wheat (Triticum aestivum L.) under salt-stressed conditions. Arch. Microbiol. 191: 5. 415-424.
Zand, A., H. Aroiee., M. R. Chaichi, and S. H. Nemati. 2017. Effects of bio-fertilizers on some physiological characteristics, essential oil percentage and yield of spearmint (Mentha spicata L.) under deficit irrigation. Iranian J.  Med. Aromat Plants, 33(1): 112-125.
Zhao, G. Q., B. L. Ma, and C. Z.  Ren. 2007. Growth, gas exchange, chlorophyll fluorescence and ion content of naked oat in response to salinity. Crop Sci. 41: 123-131.
Iranian Journal of Seed Science and Technology
Volume 10, Issue 3 - Serial Number 23
Autumn of 2021
October 2021
Pages 133-144

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